LOCUS       sequence-396818-        9617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human IGF1R receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-IGF1R-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        620..823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     774..794
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     868..887
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        868..886
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5120..5161
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5171..5188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5211..5228)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5217..5441
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5487..5915
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5574..5593)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5784..5805
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5924..5944)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5929..6258
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6109..6244
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6325..7119
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6379..6398)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6989..7008
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7295..7416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7332..7351)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7386..7405
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7465..7481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7465..7481)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7478..7500)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7489..7505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7513..7519,7520..7537,7538..7543))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7558..7579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7696..7713)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7867..8455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7947..7966)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8626..9417,9418..9486))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9249..9268
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9487..9591)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
       61 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
      121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      841 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
      901 ctagttaagc ttggtaccga gctcggatcc agtacccttc accatgaagt ctggctccgg
      961 aggagggtcc ccgacctcgc tgtgggggct cctgtttctc tccgccgcgc tctcgctctg
     1021 gccgacgagt ggagaaatct gcgggccagg catcgacatc cgcaacgact atcagcagct
     1081 gaagcgcctg gagaactgca cggtgatcga gggctacctc cacatcctgc tcatctccaa
     1141 ggccgaggac taccgcagct accgcttccc caagctcacg gtcattaccg agtacttgct
     1201 gctgttccga gtggctggcc tcgagagcct cggagacctc ttccccaacc tcacggtcat
     1261 ccgcggctgg aaactcttct acaactacgc cctggtcatc ttcgagatga ccaatctcaa
     1321 ggatattggg ctttacaacc tgaggaacat tactcggggg gccatcagga ttgagaaaaa
     1381 tgctgacctc tgttacctct ccactgtgga ctggtccctg atcctggatg cggtgtccaa
     1441 taactacatt gtggggaata agcccccaaa ggaatgtggg gacctgtgtc cagggaccat
     1501 ggaggagaag ccgatgtgtg agaagaccac catcaacaat gagtacaact accgctgctg
     1561 gaccacaaac cgctgccaga aaatgtgccc aagcacgtgt gggaagcggg cgtgcaccga
     1621 gaacaatgag tgctgccacc ccgagtgcct gggcagctgc agcgcgcctg acaacgacac
     1681 ggcctgtgta gcttgccgcc actactacta tgccggtgtc tgtgtgcctg cctgcccgcc
     1741 caacacctac aggtttgagg gctggcgctg tgtggaccgt gacttctgcg ccaacatcct
     1801 cagcgccgag agcagcgact ccgaggggtt tgtgatccac gacggcgagt gcatgcagga
     1861 gtgcccctcg ggcttcatcc gcaacggcag ccagagcatg tactgcatcc cttgtgaagg
     1921 tccttgcccg aaggtctgtg aggaagaaaa gaaaacaaag accattgatt ctgttacttc
     1981 tgctcagatg ctccaaggat gcaccatctt caagggcaat ttgctcatta acatccgacg
     2041 ggggaataac attgcttcag agctggagaa cttcatgggg ctcatcgagg tggtgacggg
     2101 ctacgtgaag atccgccatt ctcatgcctt ggtctccttg tccttcctaa aaaaccttcg
     2161 cctcatccta ggagaggagc agctagaagg gaattactcc ttctacgtcc tcgacaacca
     2221 gaacttgcag caactgtggg actgggacca ccgcaacctg accatcaaag cagggaaaat
     2281 gtactttgct ttcaatccca aattatgtgt ttccgaaatt taccgcatgg aggaagtgac
     2341 ggggactaaa gggcgccaaa gcaaagggga cataaacacc aggaacaacg gggagagagc
     2401 ctcctgtgaa agtgacgtcc tgcatttcac ctccaccacc acgtcgaaga atcgcatcat
     2461 cataacctgg caccggtacc ggccccctga ctacagggat ctcatcagct tcaccgttta
     2521 ctacaaggaa gcacccttta agaatgtcac agagtatgat gggcaggatg cctgcggctc
     2581 caacagctgg aacatggtgg acgtggacct cccgcccaac aaggacgtgg agcccggcat
     2641 cttactacat gggctgaagc cctggactca gtacgccgtt tacgtcaagg ctgtgaccct
     2701 caccatggtg gagaacgacc atatccgtgg ggccaagagt gagatcttgt acattcgcac
     2761 caatgcttca gttccttcca ttcccttgga cgttctttca gcatcgaact cctcttctca
     2821 gttaatcgtg aagtggaacc ctccctctct gcccaacggc aacctgagtt actacattgt
     2881 gcgctggcag cggcagcctc aggacggcta cctttaccgg cacaattact gctccaaaga
     2941 caaaatcccc atcaggaagt atgccgacgg caccatcgac attgaggagg tcacagagaa
     3001 ccccaagact gaggtgtgtg gtggggagaa agggccttgc tgcgcctgcc ccaaaactga
     3061 agccgagaag caggccgaga aggaggaggc tgaataccgc aaagtctttg agaatttcct
     3121 gcacaactcc atcttcgtgc ccagacctga aaggaagcgg agagatgtca tgcaagtggc
     3181 caacaccacc atgtccagcc gaagcaggaa caccacggcc gcagacacct acaacatcac
     3241 cgacccggaa gagctggaga cagagtaccc tttctttgag agcagagtgg ataacaagga
     3301 gagaactgtc atttctaacc ttcggccttt cacattgtac cgcatcgata tccacagctg
     3361 caaccacgag gctgagaagc tgggctgcag cgcctccaac ttcgtctttg caaggactat
     3421 gcccgcagaa ggagcagatg acattcctgg gccagtgacc tgggagccaa ggcctgaaaa
     3481 ctccatcttt ttaaagtggc cggaacctga gaatcccaat ggattgattc taatgtatga
     3541 aataaaatac ggatcacaag ttgaggatca gcgagaatgt gtgtccagac aggaatacag
     3601 gaagtatgga ggggccaagc taaaccggct aaacccgggg aactacacag cccggattca
     3661 ggccacatct ctctctggga atgggtcgtg gacagatcct gtgttcttct atgtccaggc
     3721 caaaacagga tatgaaaact tcatccatct gatcatcgct ctgcccgtcg ctgtcctgtt
     3781 gatcgtggga gggttggtga ttatgctgta cgtcttccat agaaagagaa ataacagcag
     3841 gctggggaat ggagtgctgt atgcctctgt gaacccggag tacttcagcg ctgctgatgt
     3901 gtacgttcct gatgagtggg aggtggctcg ggagaagatc accatgagcc gggaacttgg
     3961 gcaggggtcg tttgggatgg tctatgaagg agttgccaag ggtgtggtga aagatgaacc
     4021 tgaaaccaga gtggccatta aaacagtgaa cgaggccgca agcatgcgtg agaggattga
     4081 gtttctcaac gaagcttctg tgatgaagga gttcaattgt caccatgtgg tgcgattgct
     4141 gggtgtggtg tcccaaggcc agccaacact ggtcatcatg gaactgatga cacggggcga
     4201 tctcaaaagt tatctccggt ctctgaggcc agaaatggag aataatccag tcctagcacc
     4261 tccaagcctg agcaagatga ttcagatggc cggagagatt gcagacggca tggcatacct
     4321 caacgccaat aagttcgtcc acagagacct tgctgcccgg aattgcatgg tagccgaaga
     4381 tttcacagtc aaaatcggag attttggtat gacgcgagat atctatgaga cagactatta
     4441 ccggaaagga gggaaagggc tgctgcccgt gcgctggatg tctcctgagt ccctcaagga
     4501 tggagtcttc accacttact cggacgtctg gtccttcggg gtcgtcctct gggagatcgc
     4561 cacactggcc gagcagccct accagggctt gtccaacgag caagtccttc gcttcgtcat
     4621 ggagggcggc cttctggaca agccagacaa ctgtcctgac atgctgtttg aactgatgcg
     4681 catgtgctgg cagtataacc ccaagatgag gccttccttc ctggagatca tcagcagcat
     4741 caaagaggag atggagcctg gcttccggga ggtctccttc tactacagcg aggagaacaa
     4801 gctgcccgag ccggaggagc tggacctgga gccagagaac atggagagcg tccccctgga
     4861 cccctcggcc tcctcgtcct ccctgccact gcccgacaga cactcaggac acaaggccga
     4921 gaacggcccc ggccctgggg tgctggtcct ccgcgccagc ttcgacgaga gacagcctta
     4981 cgcccacatg aacgggggcc gcaagaacga gcgggccttg ccgctgcccc agtcttcgac
     5041 ctgcgcaaag ggtcaagaca attctgcaga tatccagcac agtggcggcc gctcgagtct
     5101 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     5161 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     5221 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5281 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5341 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5401 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5461 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5521 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5581 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5641 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5701 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5761 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5821 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5881 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5941 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6001 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6061 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6121 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6181 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6241 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     6301 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6361 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6421 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6481 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6541 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6601 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6661 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6721 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6781 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6841 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6901 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6961 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     7021 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     7081 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgcga
     7141 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     7201 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7261 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7321 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7381 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7441 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7501 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7561 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7621 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7681 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7741 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7801 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7861 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7921 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7981 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8041 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8101 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8161 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8221 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8281 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8341 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8401 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8461 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8521 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8581 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8641 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8701 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8761 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8821 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8881 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8941 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9001 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9061 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9121 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9181 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9241 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9301 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9361 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9421 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9481 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9541 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9601 ccgaaaagtg ccacctg
//
