LOCUS       sequence-396821-        9404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human INSRR receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-INSRR-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..77)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        249..628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        629..832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     783..803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     877..896
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        877..895
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(2757..2768)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4916..4957
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4967..4984
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5007..5024)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5013..5237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5283..5711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5370..5389)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5580..5601
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5720..5740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5725..6054
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5905..6040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5967..5986
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6121..6915
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6175..6194)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6785..6804
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7091..7212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7128..7147)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7182..7201
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7261..7277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7261..7277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7274..7296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7285..7301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7309..7315,7316..7333,7334..7339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7354..7375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7492..7509)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7663..8251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7743..7762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8422..9213,9214..9282))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9045..9064
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9283..9387)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
       61 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
      121 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
      181 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
      241 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
      841 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      901 ccaagctggc tagttaagct tggtaccgag ctcggatcca gtacccttca ccatggcagt
      961 ccctagtctg tggccctggg gagcatgcct gcatgtgatc ttcctctcct tgggatttgg
     1021 cctggataca gtagaggtgt gccccagcct ggatattcgc tcagaggtgg cagagcttcg
     1081 tcagctggag aactgcagcg tggtggaggg ccacctgcag atcctgctca tgttcacagc
     1141 caccggggag gacttccgcg gcctcagctt ccctcgcctc acccaggtca ccgactacct
     1201 gctgctcttc cgtgtctacg gactggagag cctgcgcgac ctcttcccca acctagcagt
     1261 catccgcggg acgcgcctct tcctgggcta tgcactggtc atctttgaga tgccacatct
     1321 gcgtgacgtg gcactgcctg cacttggggc cgtgctgcgt ggggctgtgc gtgtggagaa
     1381 gaaccaggag ctctgccacc tctccaccat tgactgggga ctgctgcagc cagcacctgg
     1441 cgccaaccac atcgtgggca acaagctggg cgaggagtgt gctgacgtgt gccctggtgt
     1501 gctgggtgct gctggtgagc cctgtgccaa gaccaccttc agcgggcaca ctgactacag
     1561 atgctggacc tccagccact gccagagagt gtgcccctgc ccccatggga tggcttgcac
     1621 agcgaggggc gagtgctgcc acaccgaatg cctggggggc tgcagccagc cagaagaccc
     1681 tcgtgcctgt gtagcttgcc gccacctcta cttccagggt gcctgcctgt gggcctgccc
     1741 gccaggcacc taccagtatg agtcctggcg ctgtgtcaca gctgagcgct gtgccagcct
     1801 gcactctgtg cccggccgtg cctccacctt cggcatacac cagggcagtt gcctggccca
     1861 gtgcccttct ggcttcaccc gtaatagcag cagcatattc tgccacaagt gcgaggggct
     1921 gtgccctaaa gagtgcaagg taggcaccaa gaccatcgac tccatccagg cggcacagga
     1981 tcttgtgggc tgcacgcatg tggagggaag cctcatcctc aaccttcgcc agggctacaa
     2041 cctggagcca cagctgcagc acagcctggg gctggtagaa accattactg gcttcctcaa
     2101 aatcaagcac tcctttgccc tcgtgtccct gggctttttc aagaacctca aactaatccg
     2161 gggagacgcc atggtggatg ggaactacac tctctacgtg ctggacaacc agaacctaca
     2221 acagctaggg tcctgggtgg ccgcggggct caccattccc gtgggcaaga tctacttcgc
     2281 cttcaacccg cgcctctgct tggaacacat ctaccgactg gaggaggtga caggcacgcg
     2341 aggtcggcag aacaaggctg agatcaaccc ccgcaccaac ggagaccgcg ccgcctgcca
     2401 gactcgcacc ctgcgcttcg tgtccaacgt gacggaggca gaccgcatcc tgctacgctg
     2461 ggagcgctat gagccactgg aggcccgcga cctgctcagc ttcatcgtgt actacaagga
     2521 gtccccattc cagaacgcca cagagcacgt gggtccagat gcttgtggaa cccagagctg
     2581 gaacctgctg gatgtggagc tgcccctaag ccgcacccag gagccagggg tgaccctagc
     2641 ctccctcaag ccttggacac agtacgcagt gtttgtgcgg gccatcacgc taaccactga
     2701 ggaggacagc cctcatcaag gagcccagag tcccatcgtc tacctccgaa cgctgcctgc
     2761 agctcccacg gtgccccaag acgtcatctc cacgtccaac tcctcctccc acctcctggt
     2821 gcgctggaag ccaccgaccc agcgcaatgg gaacctcacc tactacctgg tgctgtggca
     2881 gcggctggca gaggacggcg acctctacct caatgactac tgccaccgcg gcttgcggct
     2941 gcccaccagc aacaacgatc cgcgcttcga cggcgaagac ggggatcctg aggccgagat
     3001 ggagtccgac tgctgccctt gccagcaccc acctcctggt caggttctgc ccccgctgga
     3061 ggcgcaagag gcctcgttcc agaagaagtt tgaaaacttt ctacacaacg cgatcaccat
     3121 ccccatatcc ccttggaagg tgacgtccat caacaagagc ccccaaaggg actcagggcg
     3181 gcaccgccgg gcagctgggc ccctccggct ggggggcaac agctcggatt tcgagatcca
     3241 ggaggacaag gtgccccgtg agcgagcggt gctgagcggc ctgcgccact tcacggaata
     3301 ccggatcgac atccatgcct gcaaccacgc ggcgcacacc gtgggctgca gtgccgccac
     3361 cttcgtcttt gcgcgcacca tgccccacag agaggctgat ggtattccag gaaaggtggc
     3421 ctgggaggcc tccagcaaga acagtgtcct tctgcgctgg ctcgagccac cagaccccaa
     3481 cggactcatc ctcaagtacg aaatcaagta ccgccgcttg ggagaggagg ccacagtgct
     3541 gtgtgtgtcc cgtcttcgat atgcgaagtt tgggggagtc cacctggccc tgctgccccc
     3601 tggaaactac tctgccaggg ttagggcaac ctcactggct ggcaatggct cttggacaga
     3661 cagtgttgcc ttctacatcc ttggtccaga ggaggaggat gctggggggc tgcatgtcct
     3721 cctcactgcc acccctgtgg ggctcacgct gctcatcgtt cttgctgccc ttggtttctt
     3781 ctacggcaag aagagaaaca gaaccctgta tgcttctgtg aatccagagt acttcagcgc
     3841 ctctgatatg tatgtccctg atgaatggga ggtgcctcgg gagcagatct cgataatccg
     3901 ggaactgggc cagggctctt ttgggatggt atatgagggg ctggcacgag gacttgaggc
     3961 tggagaggag tccacacccg tggccctgaa gacggtgaat gaactggcca gcccacggga
     4021 atgcattgag ttcctcaagg aagcttctgt catgaaagcc ttcaagtgtc accatgtggt
     4081 gcgtctcctg ggtgtggtat ctcagggcca gccaactctg gtcatcatgg agttaatgac
     4141 ccgtggggac ctcaagagcc atcttcgatc tttgcggcct gaggcagaga acaaccctgg
     4201 gctcccacag ccagcattgg gggaaatgat ccaaatggct ggtgagattg cagacggcat
     4261 ggcctacctt gctgccaaca agtttgtgca ccgagatcta gcagcccgca actgcatggt
     4321 gtcccaggac ttcaccgtca agatcgggga cttcgggatg actcgggacg tgtatgagac
     4381 agactattac cgcaagggtg ggaaggggct gctgcccgtg cgctggatgg cccccgagtc
     4441 cctcaaagat gggatcttca ccacccactc ggatgtctgg tcctttggcg tggtactctg
     4501 ggagattgtg accctggcag aacaacccta ccagggcctg tccaatgagc aggtgctgaa
     4561 gttcgtcatg gatggcgggg tcctggagga gctggagggc tgtccccttc agctgcagga
     4621 gctgatgagc cgctgctggc agccgaaccc acgcctgcgc ccatctttca cacacattct
     4681 ggacagcata caggaggagc tgcggccctc cttccgcctc ctctccttct actacagccc
     4741 ggaatgccgg ggggcccggg gctccctgcc taccaccgat gcagagcctg actcctcacc
     4801 cactccaaga gactgcagcc ctcaaaatgg gggtccaggg cacaagggtc aagacaattc
     4861 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
     4921 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca
     4981 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5041 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5101 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5161 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     5221 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     5281 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5341 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5401 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5461 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5521 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5581 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5641 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5701 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5761 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5821 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5881 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5941 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     6001 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     6061 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     6121 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6181 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6241 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6301 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6361 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6421 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6481 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6541 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6601 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6661 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6721 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6781 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6841 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6901 gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa
     6961 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7021 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7081 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7141 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7201 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7261 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7321 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7381 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7441 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7501 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7561 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8101 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8281 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8341 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8401 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8461 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8521 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8581 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8641 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8701 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8761 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8821 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8881 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8941 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9001 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9061 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9121 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9181 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9241 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9301 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9361 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaag
//
