LOCUS       sequence-396863-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for ASEL neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH62 - [ASELp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1903..1919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1903..1919)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1916..1938)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2091..2679)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2171..2190)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2796..2820)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2920..3711,3712..3780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3543..3562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3781..3885)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3984..4006
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3998..4015
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3999..4015
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4639..4663,1..9))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgaagttatg atatcaaaaa tgattacgcc aagctgtaag tttaaacatg atcttactaa
       61 ctaactattc tcatttaaat tttcagagct taaaaatggc tgaaatcact cacaacgatg
      121 gatacgctaa tgcctgaaat ccccaatgtt tcagaaatgc gcatagactg atccgattaa
      181 ttacgcaatt gctaccgtaa cctaccttgt gtcacgcttt ctcatgacca acagattaat
      241 caagtccaaa agaagctttt ttctcttccg tgctttttca cgtatcaact gtttcgggag
      301 gcggaaaaat ataaaaataa ggtttcacgg attaggcggc aggcgtagta aacttaagtt
      361 tctataaatc ccaccgcccc ttgtgcagat ataaaaagat ctgctgtctg ccagtttagc
      421 ataagaaaat aaaaagagac caaaaatggg gccctctaag ggcgaagaat tgttcacggg
      481 agtagtccca atcttagtcg agcttgacgg aggtgagttt tattatcgat tttcgggatt
      541 ttctacttgt aattaccttt tgtttttaat aagttgcttt tttccagatg ttaatggcca
      601 caagttttcc gtgtctggag aaggagaagg cgacgctacc tacggtaagc tgaccctgaa
      661 gttcatttgc actactggca agttaccagt accatggccg accctggtga caacattttg
      721 ctacggggtt caatgcttct ccagataccc aggtaggttt tcagtttcaa tttttgttgc
      781 aactacctca actctatgtt ttcagatcat atgaaaagac acgacttttt taaatccgcc
      841 atgccagagg gttacgttca agaacgtacc atttttttta aggacgatgg taactacaag
      901 acacgtgcgg aggttaaatt tgaaggcgat actctcgtga accgtattga gctcaaggta
      961 aatttttcaa gcttctaaat tccaaaataa accattttaa tttcttaatt ccagggaatc
     1021 gatttcaagg aagatggaaa cattttgggc cacaagcttg aatataatta taatagtcat
     1081 aatgtctaca ttatggccga caaacaaaaa aacggtatta aggttaactt caagattcgc
     1141 cacaatatcg aagatggatc tgtccaactt gctgaccact accaacaaaa tactccaatt
     1201 ggagatggac cggttttgct tccagataac cactaccttt ccactcagtc tgccctctct
     1261 aaggatccga atgaaaaacg tgaccatatg gttcttttgg agttcgttac cgcagcagga
     1321 attactcacg gaatggatga actgtacaag cccgggtgat ggtctctgag ctatgcaaaa
     1381 tcctttcaag cattcccttc ttctctatca ctcttctttc tttttgtcaa aaaattctct
     1441 cgctaattta tttgcttttt taatgttatt attttatgac tttttatagt cactgaaaag
     1501 tttgcatctg agtgaagtga atgctatcaa aatgtgattc tgatatccag gaaacggaga
     1561 atctgtgcgg caatgtctta tcatttgaaa cgcgccgtat cgatgctgac atcatgcggc
     1621 tcccgtgtta gtcgagaaga tgggtacggg cctttattca tccaacactc tacggcttct
     1681 tcaagagcta gtagggcacc ctgtagttgg aaagggatta tttcgtatgg cgagcccata
     1741 ccgtctttct tgcggaagct taacacgata ggaagttgga tagtttcgaa cgatggttcc
     1801 atacggaacg ctgtctcaag gatgagtctg acggagtgta actcgaaggc taggtggagg
     1861 ctcagtgatg ataagtctgc gatggtggat gcatgtgtca tggtcatagc tgtttcctgt
     1921 gtgaaattgt tatccgctca gagggcacaa tcctattccg cgctatccga caatctccaa
     1981 gacattaggt ggagttcagt tcggcgtatg gcatatgtcg ctggaaagaa catgtgagca
     2041 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2101 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2161 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2221 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2281 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2341 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2401 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2461 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2521 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2581 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2641 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2701 acggggtctg acgctctatt caacaaagcc gccgtcccgt caagtcagcg taaatgggta
     2761 gggggcttca aatcgtcctc gtgataccaa ttcggagcct gcttttttgt acaaacttgt
     2821 tgataatggc aattcaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2881 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2941 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3001 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3061 agagccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3121 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3181 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3241 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3301 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3361 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3421 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3481 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3541 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3601 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3661 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3721 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3781 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3841 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3901 agtgccagat acctgaaaca aaacccatcg tacggccaag gaagtctcca ataactgtga
     3961 tccaccacaa gcgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc
     4021 ataatccgca cgcatctgga ataaggaagt gccattccgc ctgacctaag tgcttttcga
     4081 gatcgccaaa ccggagaacg atcaggagaa tgatgagtcg ccacaggagt cggcaagata
     4141 cgtgcgttat agatttgctc caaacttttt gaaactcaaa acggtcggag caactgtgga
     4201 attcaaagta ggagacatcc caatccatca tttccgaatg atcgaacgtc acttcttcaa
     4261 agatcgcctt ctgaagtgtt ttgactttga attcggattc tgtattccga attcacgaaa
     4321 caactgtgaa catatctatg agttccctca actctctcaa caactcagtg agtcattatt
     4381 ctaaaaagta caattcaaaa gactaatctc tttttcagtg gacgacatga tcaacaatcc
     4441 aaacgagact cgttctgaca gcttctattt cgtcgataac aaactcgtca tgcacaacaa
     4501 agccgactac tcatatgatg cataaatctt cattgttgag tttatcttgt tgatttttga
     4561 ataaattatc aactctttac tttttaatgg gttatgaaat aaataaacat tgaaaactga
     4621 taaacaacgt tcatctctat aacttcgtat agcatacatt ata
//
