LOCUS       sequence-396864-        4855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cbr-unc-119 rescue gene for use as co-injection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   AlHarbi S, Frokjaer-Jensen C
  TITLE     Characterizing a standardized BioPart for PVQ-specific expression in
            C. elegans.
  JOURNAL   MicroPubl Biol. 2023 Jun 22;2023:10.17912/micropub.biology.000870. 
            doi: 10.17912/micropub.biology.000870. eCollection 2023.
  PUBMED    37426742
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2023-06-22"; volume: 
            "2023:10.17912/micropub.biology.000870. doi"; pages: " 
            10.17912/micropub.biology.000870. eCollection 2023"
COMMENT     Sequence Label: pCFJ108 - pENTR221 - [cbr-unc-119(+)]
FEATURES             Location/Qualifiers
     source          1..4855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    43..142
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2294..2305
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2454..2553)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2569..2586)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2569..2585)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2578..2600)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2649..2735
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2827..2854
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3013..3030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3184..3772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3264..3283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3919..4728)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4642..4661
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4720..4739
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     join(4841..4855,1..2)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4841..4855,1..2)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 accatgtaat acgactcact ataggggata tcagctggat ggcaaataat gattttattt
       61 tgactgatag tgacctgttc gttgcaacaa attgataagc aatgctttct tataatgcca
      121 actttgtaca agaaagctgg gttaatacga ctcactagtg ggcagatctt cgaatgcatc
      181 gcgcgcaccg tacgtctcga ggaattcctg caggatatct ggatccacga agcttcccat
      241 ggtgacgtca ccggttctag atacctaggt gagctctggt accctctagt caaggcctta
      301 agtgagtcgt attacgtagc ccgggcctag ttctagacat tctctaatga aaaaatcttt
      361 cagttgaaat tgaaaatgag ttaaagttgg agtttttatt gaaaacagat ttccgtgtga
      421 ttagtgtttt tagcgagtgt gacaggacag cgaaaaaata tagaaacaag gggggaactg
      481 aaaagcttag gaatgcattg aacatgagaa ggggaagggg aaggaacaaa ctagacagga
      541 attattggaa tttaatcaca tttggagttt tttttctatt cgacagaata attatccaga
      601 acatttttgt attaaatatt tatgcatcat atgagtagtc ggctttgttg tgcatgacga
      661 gtttgttatc gacgaaatag aagctgtcag aacgagtctc gtttggattg ttgatcatgt
      721 cgtccactga aaaagagatt agtcttttga attgtacttt ttagaataat gactcactga
      781 gttgttgaga gagttgaggg aactcataga tatgttcaca gttgtttcgt gaattcggaa
      841 tacagaatcc gaattcaaag tcaaaacact tcagaaggcg atctttgaag aagtgacgtt
      901 cgatcattcg gaaatgatgg attgggatgt ctcctacttt gaattccaca gttgctccga
      961 ccgttttgag tttcaaaaag tttggagcaa atctataacg cacgtatctt gccgactcct
     1021 gtggcgactc atcattctcc tgatcgttct ccggtttggc gatctcgaaa agcacttgct
     1081 cagtgtccag atcacggatt tggaacttgg tgaactcgat gttatagatg ttcgcagatg
     1141 gggagcataa gaatcctaaa tttatgtttt aaactgaaat ccaaagggag caagatacct
     1201 tgagtgattc ccggaagtgc taaaacgtcg ttcggagtga tttgagcttt cttcgcaagc
     1261 tccgattccg ttgtgattcc ttgttcggtg cttggtggtg gccgtggcat ctggaaatat
     1321 ggaaaagttc aacaaaaaga aaagagaaaa gaatgaaatc ggatatcaag agttagttga
     1381 gcggtttctc tagttttctg agtctcacct gcgacgggaa ggtcgccgag ccgggtggaa
     1441 tcgatcgttg ttgctcggct ttcatatcgg tttggttgga agcggctgaa aacggaaaga
     1501 agtggaagaa ggaaaagagt gtggtgtgac aggaaaatgg taattagagg gtgccaaata
     1561 accagctata ttttgttttt ttttgaaaac atttttaaaa agaaaaatac gataatgata
     1621 tcagatggat ttccggaaaa ctggtatgaa aaatttcaac ctttttgagt acatgtaatc
     1681 aaaatacact ttgtaaatta tcatttttat tgaaactcca ccatttttct atttataacg
     1741 ctaataattt gaaaaagaaa cctattgcga accgcggggt gaatcccaaa aacgaatgcg
     1801 ttttggtgga gtgattgatt cgaatcgaag aagaaaaaga agaagacgtg gaatagagag
     1861 ctcactctta accgagcagc acacaccgac agaaaaaaaa atgaaatgaa tgagggtctt
     1921 cttcttcttc ttcttcgaat gattgacaga aatgggaaaa agaggaagat tgagaaggga
     1981 aaaaggaagg agaaaagaag cagaagaaga cgtcagagag gagaggaacg agcggaaaag
     2041 cagcgggcgc aagtcataga agtagcagag ctggggagaa gaagacacta tccaagaaag
     2101 gaatgacgag agagtatgca aaggggtata gggtgcagac agaataggaa cagaataaca
     2161 gatgatgagc caagaagagt tgaaaagggc gatgaatttg tcatgtaact taatttgggt
     2221 caatttgagc atgatgaatt gaaatcatcc cttgttggga gttaataacc ggtttgttat
     2281 cagaaaccct gtaatagaag ggcgccctaa ctttgagcca attcatcccg gtttctgtca
     2341 aatatatcaa aaagtggtca actgacaaat tgtttttgat attataataa acattttatc
     2401 cgttaacaat tttcgaatac tttttacaag gacttggata aattggctca aagagcctgc
     2461 ttttttgtac aaagttggca ttataaaaaa gcattgctca tcaatttgtt gcaacgaaca
     2521 ggtcactatc agtcaaaata aaatcattat ttggggcccg agcttaagac tggccgtcgt
     2581 tttacaacgt cgtgactggg aaaacatcca tgctagcgtt aacgcgagag tagggaactg
     2641 ccaggcatca aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt
     2701 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     2761 tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     2821 ctaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttcctggct
     2881 agcggtacgc gtattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2941 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3001 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3061 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3781 tttgatcttt tctacggggt ctgacgctca gtggaacgac gcgtaactca cgttaaggga
     3841 ttttggtcat gagcttgcgc cgtcccgtca agtcagcgta atgctctgcc agtgttacaa
     3901 ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     3961 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     4021 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     4081 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     4141 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     4201 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     4261 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
     4321 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     4381 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gtttttccgg ggatcgcagt
     4441 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     4501 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     4561 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaagc gatagattgt
     4621 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     4681 gttggaattt aatcgcggcc tcgacgtttc ccgttgaata tggctcataa caccccttgt
     4741 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
     4801 aatgtaacat cagagatttt gagacacggg ccagagctgc caggaaacag ctatg
//
