LOCUS       sequence-396881-        8056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Myc-beta-arrestin2-LOV-Turbo in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8056)
  AUTHORS   Lee SY, Cheah JS, Zhao B, Xu C, Roh H, Kim CK, Cho KF, Udeshi ND, 
            Carr SA, Ting AY
  TITLE     Engineered allostery in light-regulated LOV-Turbo enables precise 
            spatiotemporal control of proximity labeling in living cells.
  JOURNAL   Nat Methods. 2023 May 15. doi: 10.1038/s41592-023-01880-5.
  PUBMED    37188954
REFERENCE   2  (bases 1 to 8056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 May 15. doi: 10.1038/s41592-023-01880-5."
COMMENT     Sequence Label: Myc-beta-arrestin2-LOV-Turbo_pCDNA3
FEATURES             Location/Qualifiers
     source          1..8056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             906..935
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(3609..3627)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3610..3627)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3647..3664)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3653..3877
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3923..4351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4010..4029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4220..4241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4360..4380)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4365..4694
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4545..4680
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4607..4626
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4761..5555
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4815..4834)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5425..5444
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5729..5850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5766..5785)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5820..5839
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5899..5915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5899..5915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5912..5934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5923..5939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5947..5953,5954..5971,5972..5977))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5992..6013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6130..6147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6301..6889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6381..6400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7060..7851,7852..7920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7683..7702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7921..8025)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gcatggaaca aaagcttatt tctgaagagg acttgggctc gggcgggagt accggtggta
      961 gtgggagcgg tggtgaaaaa cccgggacca gggtcttcaa gaagtcgagc cctaactgca
     1021 agctcaccgt gtacttgggc aagcgtgact ttgtggatca cttggacaaa gtggatcctg
     1081 tcgatggtgt ggtgcttgtg gatcctgact acttgaagga ccggaaagtg tttgtgaccc
     1141 tcacctgtgc cttccgctat ggccgagaag acctggatgt actgggcctg tctttccgca
     1201 aagatctgtt catcgccacc taccaggcct tcccccccat gcccaaccca cctcggcccc
     1261 ccacccgcct acaggaccga ctgctgaaga agttgggcca gcacgcccac cccttttttt
     1321 tcacaatacc ccagaatttg ccttgctccg tcacactgca gccaggaccg gaggacacag
     1381 ggaaggcctg tggagtagac tttgagattc gagccttctg tgccaaatct atagaagaaa
     1441 aaagccacaa aaggaactcc gtgcggctta tcatcagaaa ggtacagttt gctcctgaga
     1501 cacccggccc ccagccatca gctgaaacca cacgccactt cctcatgtct gaccggaggt
     1561 ccctgcacct agaggcttcc ctggacaaag agctgtacta ccatggggaa cccctcaatg
     1621 tcaacgtcca cgtcaccaac aattctgcca aaaccgtcaa gaagatcaga gtgtctgtga
     1681 gacagtatgc cgacatttgc ctcttcagca ccgcgcagta caagtgtcct gtggctcagc
     1741 ttgaacaaga tgaccaggtg tctcccagtt ccacattctg caaggtgtac accataaccc
     1801 cgctgctcag tgacaaccga gagaagcgtg gccttgccct tgatgggcaa ctcaagcacg
     1861 aagacaccaa cctggcttcc agcaccattg tgaaggaggg agccaacaag gaggtgctgg
     1921 gaatcctagt atcctacagg gtcaaggtga agctggtggt gtctcgaggc ggggatgtct
     1981 ccgtggagct acctttcgtc ctaatgcacc ccaagcccca cgaccacatc acccttcccc
     2041 gaccccagtc agccccccgg gaaatagaca tccctgtgga taccaacctc attgaattcg
     2101 ataccaacta tgccacagac gacgacatcg tgtttgagga ctttgcgagg cttcggctga
     2161 aggggatgaa ggatgacgac tgtgatgacc agttctgcgg tggaagtgga tcaggcagcg
     2221 gtggatctaa agacaacaca gtgcctctga agctgatcgc cctcctggcc aatggagagt
     2281 tccacagcgg cgagcagctg ggcgaaaccc tgggcatgag cagagccgct atcaacaagc
     2341 acatccagac ccttcgcgac tggggcgtcg acgtgttcag cgtgcccggc aagggctaca
     2401 gcctgcctga gcctatcccc ctgctgaacg ccaagcagat tcagggccag ctggaattta
     2461 gagccaccac cctggaaaga atcgagaagt cttttgtgat caccgacccc agactgcctg
     2521 ataatcctat catcttcgtg tctgatagct tcctgcagct caccgagtat tctcgggaag
     2581 agatcctcgg aagaaattgc agattcctgc agggcccaga gacagataga gccacagtgc
     2641 ggaagatcag agatgccatc gacaaccaga cagaggtgac cgtgcagctg atcaactaca
     2701 ccaagtccgg caaaaagttc tggaacgttt tccatctcca acctatgaga gactacaagg
     2761 gcgacgtgca gtacttcatc ggagttcagc tggacggcac cgagagactg cacggcgccg
     2821 cagagcggga agctgtgtgc ctggtcaaga agaccgcctt ccagatcgcc gtggctgtgc
     2881 tgcccgtggt ggacagcaca aaccagtacc tgctggacag aatcggcgag ctgaagtccg
     2941 gcgacgcttg tatcgccgaa taccagcagg ccggcagagg cagcagaggc cggaaatggt
     3001 tcagcccctt cggcgccaac ctgtacctga gcatgttttg gcggctgaaa agaggccctg
     3061 ccgccacagg cctgggcccc gtgataggaa tcgtgatggc cgaggctctg aggaagctgg
     3121 gtgtggacaa agtgcgggtg aagtggccta acgacctgta cctgcaggac agaaagctgg
     3181 ccggcatcct ggtggaactg gctggcatca ccggcgacgc cgctcagatt gtgatcggcg
     3241 ctggcatcaa tgtggccatg agacgcgtgg aagagagcgt ggtcaaccag ggctggatca
     3301 cactgcaaga ggccggtgtg aacctggata ggaacaccct ggccgccacc ctgatcagag
     3361 agctgagagc cgctctggaa ttgtttgagc aggagggcct ggccccttac ctgccacggt
     3421 gggagaaact ggataacttc atcaacaggc ctgtgaagct gatcatcggc gataaggaaa
     3481 tcctgggaat cagccggggc atcgacaagc agggagccct gctgctggaa caagatggag
     3541 tgattaagcc ttggatgggc ggagaaatca gcctgcggtc cgccgagaag taatctagag
     3601 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
     3661 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     3721 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     3781 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     3841 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     3901 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     3961 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4021 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4081 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4141 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4201 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4261 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4321 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     4381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4441 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4501 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4561 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4621 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4681 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     4741 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4801 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4861 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4921 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     4981 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     5041 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     5101 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     5161 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     5221 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     5281 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     5341 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     5401 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5461 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5521 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5581 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     5641 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5701 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     5761 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5821 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     5881 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5941 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6001 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6061 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6541 caagctgggc tgtgtgcaca aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7201 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7621 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7681 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7741 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7801 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7861 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7921 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8041 agtgccacct gacgtc
//
