LOCUS       sequence-396889-       12234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of 3xFLAG-tagged GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Skinner OS, Blanco-Fernandez J, Goodman RP, Kawakami A, Shen H, 
            Kemeny LV, Joesch-Cohen L, Rees MG, Roth JA, Fisher DE, Mootha VK, 
            Jourdain AA
  TITLE     Salvage of ribose from uridine or RNA supports glycolysis in 
            nutrient-limited conditions.
  JOURNAL   Nat Metab. 2023 May;5(5):765-776. doi: 10.1038/s42255-023-00774-2. 
            Epub 2023 May 17.
  PUBMED    37198474
REFERENCE   2  (bases 1 to 12234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-023-00774-2"; journalName: "Nat Metab"; date: 
            "2023-05"; volume: "5"; issue: "5"; pages: "765-776"
COMMENT     Sequence Label: pWPI-GFP-3xFLAG
FEATURES             Location/Qualifiers
     source          1..12234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             join(3535..3537,3538..3540,3541..4251)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3535..3537,3538..3540,3541..4251)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3580..3601)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3841..3860)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4188..4209
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4252..4317
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4365..4932
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4383..4934
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4527..4544)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4754..4773
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4937..5731
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4991..5010)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5601..5620
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5905..6026
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6065..6653
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6118..6138)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6536..6547)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6797..6830)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6850..7030
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7055..7073)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7056..7073)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7363..7382)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7596..7615)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7715..7737
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7775..7793)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7861..7965
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7966..8034,8035..8826)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8184..8203)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8997..9585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9486..9505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9739..9756
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9826..9846)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9831..10160
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10011..10146
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11294..11359
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11489..11509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11934..12068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttcata tgtacagagg atccatggtg
     3541 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3601 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3661 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3721 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3781 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3841 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3901 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3961 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     4021 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     4081 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4141 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4201 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggattataaa
     4261 gatcatgatg gcgattataa agatcatgat attgattata aagatgatga tgataaataa
     4321 tagtgagcgg ccgcactagt cccgggacta cgggctgcag gaattccgnc cccccccccc
     4381 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     4441 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     4501 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     4561 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     4621 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     4681 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     4741 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     4801 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     4861 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     4921 acacgatgat aataccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4981 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5041 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5101 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5161 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5221 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5281 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5341 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5401 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5461 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5521 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5581 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5641 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5701 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     5761 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5821 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5881 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     5941 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6001 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc gagggaattc
     6061 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6121 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6181 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6241 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6301 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6361 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6421 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     6481 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6541 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6601 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggga
     6661 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6721 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatggga
     6781 tcaattcacc atgggaataa cttcgtatag catacattat acgaagttat gctgcttttt
     6841 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     6901 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     6961 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7021 atctctagca gcatctagaa ttaattccgt gtattctata gtgtcaccta aatcgtatgt
     7081 gtatgataca taaggttatg tattaattgt agccgcgttc taacgacaat atgtacaagc
     7141 ctaattgtgt agcatctggc ttactgaagc agaccctatc atctctctcg taaactgccg
     7201 tcagagtcgg tttggttgga cgaaccttct gagtttctgg taacgccgtc ccgcacccgg
     7261 aaatggtcag cgaaccaatc agcagggtca tcgctagcca gatcctctac gccggacgca
     7321 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     7381 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     7441 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     7501 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     7561 cgcataaggg agagcgtcga atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7621 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7681 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7741 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     7801 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     7861 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7921 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7981 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8041 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8101 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8161 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8221 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8281 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8341 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8401 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8461 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8521 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8581 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8641 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8701 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8761 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8821 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8881 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8941 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9001 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9061 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9121 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9181 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9241 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9301 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacaacctac
     9361 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9421 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9481 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9541 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9601 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9661 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9721 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     9781 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
     9841 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9901 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9961 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    10021 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    10081 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10141 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
    10201 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
    10261 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
    10321 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
    10381 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
    10441 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
    10501 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
    10561 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
    10621 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
    10681 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
    10741 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
    10801 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
    10861 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
    10921 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
    10981 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
    11041 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
    11101 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
    11161 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat
    11221 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
    11281 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
    11341 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
    11401 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
    11461 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
    11521 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
    11581 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
    11641 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
    11701 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
    11761 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
    11821 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
    11881 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    11941 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12001 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    12061 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    12121 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
    12181 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtag
//
