LOCUS       sequence-396913-       18608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18608)
  AUTHORS   Qian F, Boletta A, Bhunia AK, Xu H, Liu L, Ahrabi AK, Watnick TJ, 
            Zhou F, Germino GG
  TITLE     Cleavage of polycystin-1 requires the receptor for egg jelly domain 
            and is disrupted by human autosomal-dominant polycystic kidney 
            disease 1-associated mutations.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Dec 24. 99(26):16981-6.
  PUBMED    12482949
REFERENCE   2  (bases 1 to 18608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Dec 24. 99(26):16981-6."
COMMENT     Sequence Label: GFP-WT (AF41)
FEATURES             Location/Qualifiers
     source          1..18608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        828..1207
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1208..1411
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1408..1432
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1547..1679
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1662..1681
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1724..1742
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          1773..2345
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     2353..2372
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(2691..2693,2694..2696,2697..3407)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2691..2693,2694..2696,2697..3407)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2736..2757)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2997..3016)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3344..3365
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             16248..16271
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    16331..16452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(16342..16361)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     16396..16415
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      16686..17141
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16773..16792)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16983..17004
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(17208..17227)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17327..17349
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(17387..17405)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        17473..17577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(17578..17646,17647..18438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(17796..17815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgg ctcgacagat
      661 cgatctggcc aaaaaggccg gaccggatct tcaatattgg ccattagcca tattattcat
      721 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
      781 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttggc attgattatt
      841 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      901 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      961 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1021 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1081 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1141 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1201 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
     1261 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1321 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
     1381 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcactagaag
     1441 ctttattgcg gtagtttatc acagttaaat tgctaacgca gtcagtgctt ctgacacaac
     1501 agtctcgaac ttaagctgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt
     1561 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt
     1621 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg
     1681 tgtccactcc cagttcaatt acagctctta aggctagagt acttaatacg actcactata
     1741 ggctagtctg gatcgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt
     1801 tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt
     1861 ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc
     1921 actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt
     1981 tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt
     2041 cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt
     2101 gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt agaatatttc
     2161 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg
     2221 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa
     2281 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc
     2341 tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg
     2401 cggccgcgtc gacagcgtcc gagcgggcgg ccgagctccc ggagcggcct ggccccgagc
     2461 cccgagcggg cgtcgctcag cagcaggtcg cggccgcagc cccatccagc cccgcgcccg
     2521 ccatgccgtc cgcgggcccc gcctgagctg cggcctccgc gcgcgggcgg gcctggggac
     2581 ggcggggcca tgcgcgcgct gccctaacga tgccgcccgc cgcgcccgcc cgcctggcgc
     2641 tggccctggg cctgggcctg tggctcgggg cgctggcggg gggccccggc atggtgagca
     2701 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     2761 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     2821 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     2881 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     2941 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3001 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3061 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3121 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     3181 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3241 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     3301 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3361 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtac tcagatcccg
     3421 ggcgcggctg cgggccctgc gagcccccct gcctctgcgg cccagcgccc ggcgccgcct
     3481 gccgcgtcaa ctgctcgggc cgcgggctgc ggacgctcgg tcccgcgctg cgcatccccg
     3541 cggacgccac agcgctagac gtctcccaca acctgctccg ggcgctggac gttgggctcc
     3601 tggcgaacct ctcggcgctg gcagagctgg atataagcaa caacaagatt tctacgttag
     3661 aagaaggaat atttgctaat ttatttaatt taagtgaaat aaacctgagt gggaacccgt
     3721 ttgagtgtga ctgtggcctg gcgtggctgc cgcgatgggc ggaggagcag caggtgcggg
     3781 tggtgcagcc cgaggcagcc acgtgtgctg ggcctggctc cctggctggc cagcctctgc
     3841 ttggcatccc cttgctggac agtggctgtg gtgaggagta tgtcgcctgc ctccctgaca
     3901 acagctcagg caccgtggca gcagtgtcct tttcagctgc ccacgaaggc ctgcttcagc
     3961 cagaggcctg cagcgccttc tgcttctcca ccggccaggg cctcgcagcc ctctcggagc
     4021 agggctggtg cctgtgtggg gcggcccagc cctccagtgc ctcctttgcc tgcctgtccc
     4081 tctgctccgg ccccccgcca cctcctgccc ccacctgtag gggccccacc ctcctccagc
     4141 acgtcttccc tgcctcccca ggggccaccc tggtggggcc ccacggacct ctggcctctg
     4201 gccagctagc agccttccac atcgctgccc cgctccctgt cactgccaca cgctgggact
     4261 tcggagacgg ctccgccgag gtggatgccg ctgggccggc tgcctcgcat cgctatgtgc
     4321 tgcctgggcg ctatcacgtg acggccgtgc tggccctggg ggccggctca gccctgctgg
     4381 ggacagacgt gcaggtggaa gcggcacctg ccgccctgga gctcgtgtgc ccgtcctcgg
     4441 tgcagagtga cgagagcctt gacctcagca tccagaaccg cggtggttca ggcctggagg
     4501 ccgcctacag catcgtggcc ctgggcgagg agccggcccg agcggtgcac ccgctctgcc
     4561 cctcggacac ggagatcttc cctggcaacg ggcactgcta ccgcctggtg gtggagaagg
     4621 cggcctggct gcaggcgcag gagcagtgtc aggcctgggc cggggccgcc ctggcaatgg
     4681 tggacagtcc cgccgtgcag cgcttcctgg tctctcgggt caccaggagc ctagacgtgt
     4741 ggatcggctt ctcgactgtg cagggggtgg aggtgggccc agcgccgcag ggcgaggcct
     4801 tcagcctgga gagctgccag aactggctgc ccggggagcc acacccagcc acagccgagc
     4861 actgcgtccg gctcgggccc accgggtggt gtaacaccga cctgtgctca gcgccgcaca
     4921 gctacgtctg cgagctgcag cccggaggcc cagtgcagga tgccgagaac ctcctcgtgg
     4981 gagcgcccag tggggacctg cagggacccc tgacgcctct ggcacagcag gacggcctct
     5041 cagccccgca cgagcccgtg gaggtcatgg tattcccggg cctgcgtctg agccgtgaag
     5101 ccttcctcac cacggccgaa tttgggaccc aggagctccg gcggcccgcc cagctgcggc
     5161 tgcaggtgta ccggctcctc agcacagcag ggaccccgga gaacggcagc gagcctgaga
     5221 gcaggtcccc ggacaacagg acccagctgg cccccgcgtg catgccaggg ggacgctggt
     5281 gccctggagc caacatctgc ttgccgctgg acgcctcctg ccacccccag gcctgcgcca
     5341 atggctgcac gtcagggcca gggctacccg gggcccccta tgcgctatgg agagagttcc
     5401 tcttctccgt tcccgcgggg ccccccgcgc agtactcggt caccctccac ggccaggatg
     5461 tcctcatgct ccctggtgac ctcgttggct tgcagcacga cgctggccct ggcgccctcc
     5521 tgcactgctc gccggctccc ggccaccctg gtccccaggc cccgtacctc tccgccaacg
     5581 cctcgtcatg gctgccccac ttgccagccc agctggaggg cacttgggcc tgccctgcct
     5641 gtgccctgcg gctgcttgca gccacggaac agctcaccgt gctgctgggc ttgaggccca
     5701 accctggact gcggctgcct gggcgctatg aggtccgggc agaggtgggc aatggcgtgt
     5761 ccaggcacaa cctctcctgc agctttgacg tggtctcccc agtggctggg ctgcgggtca
     5821 tctaccctgc cccccgcgac ggccgcctct acgtgcccac caacggctca gccttggtgc
     5881 tccaggtgga ctctggtgcc aacgccacgg ccacggctcg ctggcctggg ggcagtgtca
     5941 gcgcccgctt tgagaatgtc tgccctgccc tggtggccac cttcgtgccc ggctgcccct
     6001 gggagaccaa cgataccctg ttctcagtgg tagcactgcc gtggctcagt gagggggagc
     6061 acgtggtgga cgtggtggtg gaaaacagcg ccagccgggc caacctcagc ctgcgggtga
     6121 cggcggagga gcccatctgt ggcctccgcg ccacgcccag ccccgaggcc cgtgtactgc
     6181 agggagtcct agtgaggtac agccccgtgg tggaggccgg ctcggacatg gtcttccggt
     6241 ggaccatcaa cgacaagcag tccctgacct tccagaacgt ggtcttcaat gtcatttatc
     6301 agagcgcggc ggtcttcaag ctctcactga cggcccccaa ccacgtgagc aacgtcaccg
     6361 tgaactacaa cgtaaccgtg gagcggatga acaggatgca gggtctgcag gtctccacag
     6421 tgccggccgt gctgtccccc aatgccacgc tggcactgac ggcgggcgtg ctggtggact
     6481 cggccgtgga ggtggccttc ctgtggacct ttggggatgg ggagcaggcc ctccaccagt
     6541 tccagcctcc gtacaacgag tccttcccgg ttccagaccc ctcggtggcc caggtgctgg
     6601 tggagcacaa tgtcatgcac acctacgctg ccccaggtga gtacctcctg accgtgctgg
     6661 catctaatgc cttcgagaac ctgacgcagc aggtgcctgt gagcgtgcgc gcctccctgc
     6721 cctccgtggc tgtgggtgtg agtgacggcg tcctggtggc cggccggccc gtcaccttct
     6781 acccgcaccc gctgccctcg cctgggggtg ttctttacac gtgggacttc ggggacggct
     6841 cccctgtcct gacccagagc cagccggctg ccaaccacac ctatgcctcg aggggcacct
     6901 accacgtgcg cctggaggtc aacaacacgg tgagcggtgc ggcggcccag gcggatgtgc
     6961 gcgtctttga ggagctccgc ggactcagcg tggacatgag cctggccgtg gagcagggcg
     7021 cccccgtggt ggtcagcgcc gcggtgcaga cgggcgacaa catcacgtgg accttcgaca
     7081 tgggggacgg caccgtgctg tcgggcccgg aggcaacagt ggagcatgtg tacctgcggg
     7141 cacagaactg cacagtgacc gtgggtgcgg ccagccccgc cggccacctg gcccggagcc
     7201 tgcacgtgct ggtcttcgtc ctggaggtgc tgcgcgttga acccgccgcc tgcatcccca
     7261 cgcagcctga cgcgcggctc acggcctacg tcaccgggaa cccggcccac tacctcttcg
     7321 actggacctt cggggatggc tcctccaaca cgaccgtgcg ggggtgcccg acggtgacac
     7381 acaacttcac gcggagcggc acgttccccc tggcgctggt gctgtccagc cgcgtgaaca
     7441 gggcgcatta cttcaccagc atctgcgtgg agccagaggt gggcaacgtc accctgcagc
     7501 cagagaggca gtttgtgcag ctcggggacg aggcctggct ggtggcatgt gcctggcccc
     7561 cgttccccta ccgctacacc tgggactttg gcaccgagga agccgccccc acccgtgcca
     7621 ggggccctga ggtgacgttc atctaccgag acccaggctc ctatcttgtg acagtcaccg
     7681 cgtccaacaa catctctgct gccaatgact cagccctggt ggaggtgcag gagcccgtgc
     7741 tggtcaccag catcaaggtc aatggctccc ttgggctgga gctgcagcag ccgtacctgt
     7801 tctctgctgt gggccgtggg cgccccgcca gctacctgtg ggatctgggg gacggtgggt
     7861 ggctcgaggg tccggaggtc acccacgctt acaacagcac aggtgacttc accgttaggg
     7921 tggccggctg gaatgaggtg agccgcagcg aggcctggct caatgtgacg gtgaagcggc
     7981 gcgtgcgggg gctcgtcgtc aatgcaagcc gcacggtggt gcccctgaat gggagcgtga
     8041 gcttcagcac gtcgctggag gccggcagtg atgtgcgcta ttcctgggtg ctctgtgacc
     8101 gctgcacgcc catccctggg ggtcctacca tctcttacac cttccgctcc gtgggcacct
     8161 tcaatatcat cgtcacggct gagaacgagg tgggctccgc ccaggacagc atcttcgtct
     8221 atgtcctgca gctcatagag gggctgcagg tggtgggcgg tggccgctac ttccccacca
     8281 accacacggt acagctgcag gccgtggtta gggatggcac caacgtctcc tacagctgga
     8341 ctgcctggag ggacaggggc ccggccctgg ccggcagcgg caaaggcttc tcgctcaccg
     8401 tgctcgaggc cggcacctac catgtgcagc tgcgggccac caacatgctg ggcagcgcct
     8461 gggccgactg caccatggac ttcgtggagc ctgtggggtg gctgatggtg gccgcctccc
     8521 cgaacccagc tgccgtcaac acaagcgtca ccctcagtgc cgagctggct ggtggcagtg
     8581 gtgtcgtata cacttggtcc ttggaggagg ggctgagctg ggagacctcc gagccattta
     8641 ccacccatag cttccccaca cccggcctgc acttggtcac catgacggca gggaacccgc
     8701 tgggctcagc caacgccacc gtggaagtgg atgtgcaggt gcctgtgagt ggcctcagca
     8761 tcagggccag cgagcccgga ggcagcttcg tggcggccgg gtcctctgtg cccttttggg
     8821 ggcagctggc cacgggcacc aatgtgagct ggtgctgggc tgtgcccggc ggcagcagca
     8881 agcgtggccc tcatgtcacc atggtcttcc cggatgctgg caccttctcc atccggctca
     8941 atgcctccaa cgcagtcagc tgggtctcag ccacgtacaa cctcacggcg gaggagccca
     9001 tcgtgggcct ggtgctgtgg gccagcagca aggtggtggc gcccgggcag ctggtccatt
     9061 ttcagatcct gctggctgcc ggctcagctg tcaccttccg cctgcaggtc ggcggggcca
     9121 accccgaggt gctccccggg ccccgtttct cccacagctt cccccgcgtc ggagaccacg
     9181 tggtgagcgt gcggggcaaa aaccacgtga gctgggccca ggcgcaggtg cgcatcgtgg
     9241 tgctggaggc cgtgagtggg ctgcaggtgc ccaactgctg cgagcctggc atcgccacgg
     9301 gcactgagag gaacttcaca gcccgcgtgc agcgcggctc tcgggtcgcc tacgcctggt
     9361 acttctcgct gcagaaggtc cagggcgact cgctggtcat cctgtcgggc cgcgacgtca
     9421 cctacacgcc cgtggccgcg gggctgttgg agatccaggt gcgcgccttc aacgccctgg
     9481 gcagtgagaa ccgcacgctg gtgctggagg ttcaggacgc cgtccagtat gtggccctgc
     9541 agagcggccc ctgcttcacc aaccgctcgg cgcagtttga ggccgccacc agccccagcc
     9601 cccggcgtgt ggcctaccac tgggactttg gggatgggtc gccagggcag gacacagatg
     9661 agcccagggc cgagcactcc tacctgaggc ctggggacta ccgcgtgcag gtgaacgcct
     9721 ccaacctggt gagcttcttc gtggcgcagg ccacggtgac cgtccaggtg ctggcctgcc
     9781 gggagccgga ggtggacgtg gtcctgcccc tgcaggtgct gatgcggcga tcacagcgca
     9841 actacttgga ggcccacgtt gacctgcgcg actgcgtcac ctaccagact gagtaccgct
     9901 gggaggtgta tcgcaccgcc agctgccagc ggccggggcg cccagcgcgt gtggccctgc
     9961 ccggcgtgga cgtgagccgg cctcggctgg tgctgccgcg gctggcgctg cctgtggggc
    10021 actactgctt tgtgtttgtc gtgtcatttg gggacacgcc actgacacag agcatccagg
    10081 ccaatgtgac ggtggccccc gagcgcctgg tgcccatcat tgagggtggc tcataccgcg
    10141 tgtggtcaga cacacgggac ctggtgctgg atgggagcga gtcctacgac cccaacctgg
    10201 aggacggcga ccagacgccg ctcagtttcc actgggcctg tgtggcttcg acacagaggg
    10261 aggctggcgg gtgtgcgctg aactttgggc cccgcgggag cagcacggtc accattccac
    10321 gggagcggct ggcggctggc gtggagtaca ccttcagcct gaccgtgtgg aaggccggcc
    10381 gcaaggagga ggccaccaac cagacggtgc tgatccggag tggccgggtg cccattgtgt
    10441 ccttggagtg tgtgtcctgc aaggcacagg ccgtgtacga agtgagccgc agctcctacg
    10501 tgtacttgga gggccgctgc ctcaattgca gcagcggctc caagcgaggg cggtgggctg
    10561 cacgtacgtt cagcaacaag acgctggtgc tggatgagac caccacatcc acgggcagtg
    10621 caggcatgcg actggtgctg cggcggggcg tgctgcggga cggcgaggga tacaccttca
    10681 cgctcacggt gctgggccgc tctggcgagg aggagggctg cgcctccatc cgcctgtccc
    10741 ccaaccgccc gccgctgggg ggctcttgcc gcctcttccc actgggcgct gtgcacgccc
    10801 tcaccaccaa ggtgcacttc gaatgcacgg gctggcatga cgcggaggat gctggcgccc
    10861 cgctggtgta cgccctgctg ctgcggcgct gtcgccaggg ccactgcgag gagttctgtg
    10921 tctacaaggg cagcctctcc agctacggag ccgtgctgcc cccgggtttc aggccacact
    10981 tcgaggtggg cctggccgtg gtggtgcagg accagctggg agccgctgtg gtcgccctca
    11041 acaggtcttt ggccatcacc ctcccagagc ccaacggcag cgcaacgggg ctcacagtct
    11101 ggctgcacgg gctcaccgct agtgtgctcc cagggctgct gcggcaggcc gatccccagc
    11161 acgtcatcga gtactcgttg gccctggtca ccgtgctgaa cgagtacgag cgggccctgg
    11221 acgtggcggc agagcccaag cacgagcggc agcaccgagc ccagatacgc aagaacatca
    11281 cggagactct ggtgtccctg agggtccaca ctgtggatga catccagcag atcgctgctg
    11341 cgctggccca gtgcatgggg cccagcaggg agctcgtatg ccgctcgtgc ctgaagcaga
    11401 cgctgcacaa gctggaggcc atgatgctca tcctgcaggc agagaccacc gcgggcaccg
    11461 tgacgcccac cgccatcgga gacagcatcc tcaacatcac aggagacctc atccacctgg
    11521 ccagctcgga cgtgcgggca ccacagccct cagagctggg agccgagtca ccatctcgga
    11581 tggtggcgtc ccaggcctac aacctgacct ctgccctcat gcgcatcctc atgcgctccc
    11641 gcgtgctcaa cgaggagccc ctgacgctgg cgggcgagga gatcgtggcc cagggcaagc
    11701 gctcggaccc gcggagcctg ctgtgctatg gcggcgcccc agggcctggc tgccacttct
    11761 ccatccccga ggctttcagc ggggccctgg ccaacctcag tgacgtggtg cagctcatct
    11821 ttctggtgga ctccaatccc tttccctttg gctatatcag caactacacc gtctccacca
    11881 aggtggcctc gatggcattc cagacacagg ccggcgccca gatccccatc gagcggctgg
    11941 cctcagagcg cgccatcacc gtgaaggtgc ccaacaactc ggactgggct gcccggggcc
    12001 accgcagctc cgccaactcc gccaactccg ttgtggtcca gccccaggcc tccgtcggtg
    12061 ctgtggtcac cctggacagc agcaaccctg cggccgggct gcatctgcag ctcaactata
    12121 cgctgctgga cggccactac ctgtctgagg aacctgagcc ctacctggca gtctacctac
    12181 actcggagcc ccggcccaat gagcacaact gctcggctag caggaggatc cgcccagagt
    12241 cactccaggg tgctgaccac cggccctaca ccttcttcat ttccccgggg agcagagacc
    12301 cagcggggag ttaccatctg aacctctcca gccacttccg ctggtcggcg ctgcaggtgt
    12361 ccgtgggcct gtacacgtcc ctgtgccagt acttcagcga ggaggacatg gtgtggcgga
    12421 cagaggggct gctgcccctg gaggagacct cgccccgcca ggccgtctgc ctcacccgcc
    12481 acctcaccgc cttcggcgcc agcctcttcg tgcccccaag ccatgtccgc tttgtgtttc
    12541 ctgagccgac agcggatgta aactacatcg tcatgctgac atgtgctgtg tgcctggtga
    12601 cctacatggt catggccgcc atcctgcaca agctggacca gttggatgcc agccggggcc
    12661 gcgccatccc tttctgtggg cagcggggcc gcttcaagta cgagatcctc gtcaagacag
    12721 gctggggccg gggctcaggt accacggccc acgtgggcat catgctgtat ggggtggaca
    12781 gccggagcgg ccaccggcac ctggacggcg acagagcctt ccaccgcaac agcctggaca
    12841 tcttccggat cgccaccccg cacagcctgg gtagcgtgtg gaagatccga gtgtggcacg
    12901 acaacaaagg gctcagccct gcctggttcc tgcagcacgt catcgtcagg gacctgcaga
    12961 cggcacgcag cgccttcttc ctggtcaatg actggctttc ggtggagacg gaggccaacg
    13021 ggggcctggt ggagaaggag gtgctggccg cgagcgacgc agcccttttg cgcttccggc
    13081 gcctgctggt ggctgagctg cagcgtggct tctttgacaa gcacatctgg ctctccatat
    13141 gggaccggcc gcctcgtagc cgtttcactc gcatccagag ggccacctgc tgcgttctcc
    13201 tcatctgcct cttcctgggc gccaacgccg tgtggtacgg ggctgttggc gactctgcct
    13261 acagcacggg gcatgtgtcc aggctgagcc cgctgagcgt cgacacagtc gctgttggcc
    13321 tggtgtccag cgtggttgtc tatcccgtct acctggccat cctttttctc ttccggatgt
    13381 cccggagcaa ggtggctggg agcccgagcc ccacacctgc cgggcagcag gtgctggaca
    13441 tcgacagctg cctggactcg tccgtgctgg acagctcctt cctcacgttc tcaggcctcc
    13501 acgctgaggc ctttgttgga cagatgaaga gtgacttgtt tctggatgat tctaagagtc
    13561 tggtgtgctg gccctccggc gagggaacgc tcagttggcc ggacctgctc agtgacccgt
    13621 ccattgtggg tagcaatctg cggcagctgg cacggggcca ggcgggccat gggctgggcc
    13681 cagaggagga cggcttctcc ctggccagcc cctactcgcc tgccaaatcc ttctcagcat
    13741 cagatgaaga cctgatccag caggtccttg ccgagggggt cagcagccca gcccctaccc
    13801 aagacaccca catggaaacg gacctgctca gcagcctgtc cagcactcct ggggagaaga
    13861 cagagacgct ggcgctgcag aggctggggg agctggggcc acccagccca ggcctgaact
    13921 gggaacagcc ccaggcagcg aggctgtcca ggacaggact ggtggagggt ctgcggaagc
    13981 gcctgctgcc ggcctggtgt gcctccctgg cccacgggct cagcctgctc ctggtggctg
    14041 tggctgtggc tgtctcaggg tgggtgggtg cgagcttccc cccgggcgtg agtgttgcgt
    14101 ggctcctgtc cagcagcgcc agcttcctgg cctcattcct cggctgggag ccactgaagg
    14161 tcttgctgga agccctgtac ttctcactgg tggccaagcg gctgcacccg gatgaagatg
    14221 acaccctggt agagagcccg gctgtgacgc ctgtgagcgc acgtgtgccc cgcgtacggc
    14281 caccccacgg ctttgcactc ttcctggcca aggaagaagc ccgcaaggtc aagaggctac
    14341 atggcatgct gcggagcctc ctggtgtaca tgctttttct gctggtgacc ctgctggcca
    14401 gctatgggga tgcctcatgc catgggcacg cctaccgtct gcaaagcgcc atcaagcagg
    14461 agctgcacag ccgggccttc ctggccatca cgcggtctga ggagctctgg ccatggatgg
    14521 cccacgtgct gctgccctac gtccacggga accagtccag cccagagctg gggcccccac
    14581 ggctgcggca ggtgcggctg caggaagcac tctacccaga ccctcccggc cccagggtcc
    14641 acacgtgctc ggccgcagga ggcttcagca ccagcgatta cgacgttggc tgggagagtc
    14701 ctcacaatgg ctcggggacg tgggcctatt cagcgccgga tctgctgggg gcatggtcct
    14761 ggggctcctg tgccgtgtat gacagcgggg gctacgtgca ggagctgggc ctgagcctgg
    14821 aggagagccg cgaccggctg cgcttcctgc agctgcacaa ctggctggac aacaggagcc
    14881 gcgctgtgtt cctggagctc acgcgctaca gcccggccgt ggggctgcac gccgccgtca
    14941 cgctgcgcct cgagttcccg gcggccggcc gcgccctggc cgccctcagc gtccgcccct
    15001 ttgcgctgcg ccgcctcagc gcgggcctct cgctgcctct gctcacctcg gtgtgcctgc
    15061 tgctgttcgc cgtgcacttc gccgtggccg aggcccgtac ttggcacagg gaagggcgct
    15121 ggcgcgtgct gcggctcgga gcctgggcgc ggtggctgct ggtggcgctg acggcggcca
    15181 cggcactggt acgcctcgcc cagctgggtg ccgctgaccg ccagtggacc cgtttcgtgc
    15241 gcggccgccc gcgccgcttc actagcttcg accaggtggc gcagctgagc tccgcagccc
    15301 gtggcctggc ggcctcgctg ctcttcctgc ttttggtcaa ggctgcccag cagctacgct
    15361 tcgtgcgcca gtggtccgtc tttggcaaga cattatgccg agctctgcca gagctcctgg
    15421 gggtcacctt gggcctggtg gtgctcgggg tagcctacgc ccagctggcc atcctgctcg
    15481 tgtcttcctg tgtggactcc ctctggagcg tggcccaggc cctgttggtg ctgtgccctg
    15541 ggactgggct ctctaccctg tgtcctgccg agtcctggca cctgtcaccc ctgctgtgtg
    15601 tggggctctg ggcactgcgg ctgtggggcg ccctacggct gggggctgtt attctccgct
    15661 ggcgctacca cgccttgcgt ggagagctgt accggccggc ctgggagccc caggactacg
    15721 agatggtgga gttgttcctg cgcaggctgc gcctctggat gggcctcagc aaggtcaagg
    15781 agttccgcca caaagtccgc tttgaaggga tggagccgct gccctctcgc tcctccaggg
    15841 gctccaaggt atccccggat gtgcccccac ccagcgctgg ctccgatgcc tcgcacccct
    15901 ccacctcctc cagccagctg gatgggctga gcgtgagcct gggccggctg gggacaaggt
    15961 gtgagcctga gccctcccgc ctccaagccg tgttcgaggc cctgctcacc cagtttgacc
    16021 gactcaacca ggccacagag gacgtctacc agctggagca gcagctgcac agcctgcaag
    16081 gccgcaggag cagccgggcg cccgccggat cttcccgtgg cccatccccg ggcctgcggc
    16141 cagcactgcc cagccgcctt gcccgggcca gtcggggtgt ggacctggcc actggcccca
    16201 gcaggacacc ccttcgggcc aagaacaagg tccaccccag cagcactgac tacaaggacg
    16261 acgatgacaa gtgatcaggt agcttatcga taccgtcgac ccgggcggcc gccgcttcga
    16321 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
    16381 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
    16441 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
    16501 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatcaa
    16561 ttgccttttt ttttaatttt tattttattt tatttttgag atggccagat ccgggctggc
    16621 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    16681 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    16741 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    16801 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    16861 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    16921 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    16981 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    17041 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    17101 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
    17161 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
    17221 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
    17281 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
    17341 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
    17401 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
    17461 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
    17521 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
    17581 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
    17641 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    17701 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    17761 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    17821 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    17881 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    17941 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    18001 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    18061 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    18121 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    18181 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    18241 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    18301 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    18361 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    18421 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    18481 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    18541 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    18601 ggatcttc
//
