LOCUS       sequence-396925-        6257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmDCP1_327-372.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Tritschler F, Braun JE, Motz C, Igreja C, Haas G, Truffault V, 
            Izaurralde E, Weichenrieder O
  TITLE     DCP1 forms asymmetric trimers to assemble into active mRNA decapping
            complexes in metazoa.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6.
  PUBMED    19966221
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Dec 22. 106(51):21591-6."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H
FEATURES             Location/Qualifiers
     source          1..6257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     57..75
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        139..2648
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2668..2733
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2758..2784
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2950..3603
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             3646..3687
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3697..3714
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3737..3754)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3787..3921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3811..3830)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3865..3884
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4522..5110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4602..4621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5281..6072,6073..6141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5904..5923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6142..6246)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
       61 ggcgtatcac gaggcccttt cgtcttcaag aattaattct atattctaaa aacacaaatg
      121 atacttctaa aaaaaaatca tgaatggcat caactctgaa tcaaatcttt gcagatgcac
      181 ctacttctca tttccactgt cacatcattt ttccagatct cgctgcctgt tatgtggccc
      241 acaaaccaag acacgtttta tggccattaa agctggctga tcgtcgccaa acaccaaata
      301 cataatgaat atgtacacat tcgagaaaga agcgatcaaa gaagcgtctt cgggcggagt
      361 aggagaatgc ggaggagaag gagaacgagc tgatctagta tctctccaca atccaatgcc
      421 aactgaccaa ctggccatat tcggagcaat ttgaagccaa tttccatcgc ctggcgatcg
      481 ctccattctt ggctatatgt ttttcaccgt tacccggggc cattttcaaa gactcgtcgg
      541 caagataaga ttgtgtcact cgctgtctct cttcatttgt cgaagaatgc tgaggaattt
      601 cgcgatgacg tcggcgagta ttttgaagaa tgagaataat ttgtatttat acgaaaatca
      661 gttagtggaa ttttctacaa aaacatgtta tctatagata attttgttgc aaaatatgtt
      721 gactatgaca aagattgtat gtatatacct ttaatgtatt ctcattttct tatgtattta
      781 taatggcaat gatgatactg atgatatttt aagatgatgc cagaccaaaa ggcttgaatt
      841 tctgcgtctt ttgccgaacg cagtgcatgt gcaattgttg ttttttggaa tattcaattt
      901 tcggactgtc cgctttgatt tcagtttctt ggcttattca aaaagcaaag taaagccaaa
      961 aaagcgagat ggcaatacca aatgcggcaa aacggtagtg gaaggaaagg ggtgcggggc
     1021 agcggaagga agggtggggc ggggcgtggc ggggtctgtg gctgggcgcg acgtcaccga
     1081 cgttggagcc actcctttga ccatgtgtgc gtgtgtgtat tattcgtgtc tcgccactcg
     1141 ccggttgttt ttttcttttt atgctgcgct ctctctagcg ccatctcgct tacgcatgct
     1201 caacgcaccg catgttgccg tttcctttta tgcgtcattt tggctcgaaa taggcaatta
     1261 tttaaacaaa gattagtcaa cgaaaacgct aaaataaata agtctacaat atggttactt
     1321 attgccatgt gtgtgcagcc aacgatagca acaaaagcaa caacacaggt ggctttccct
     1381 ctttcacttt ttgtttgcaa gccgcgtgcg agcaagacgg cacgaccggc aaacgcaatt
     1441 acgctgacaa agagcagacg aagttttggc gaaaaacatc aaggcgcctg atacgaatgc
     1501 atttgcaata acaattgcga tatttaatat tgtttatgaa gctgtttgac ttcaaaacac
     1561 acaaaaaaaa aaataaaaca aattatttga aagagaatta ggaatcggac gcttatcgtt
     1621 agggtaacaa caagaaatgc ttactgagtc acagcctctg gaaaactgcc gcaagccaga
     1681 gagagagaga aaaagaggga gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc
     1741 tctcttcgtc tctgttgcgc aaacgcatag actgcactga aaaaatcgat tacctatttt
     1801 ttatgaatga atatttgcac tattactatt caaaactatt aagatagcaa tcacattcaa
     1861 tagccaaata ctataccacc tgagcgatgc aacgaaatga tcaatttgag caaaaatgct
     1921 gcatatttag gacggcatca ttatagaaat gcttcttgct gtgtactttt ctctcgtctg
     1981 gcagctgttt cgccgttatt gttaaaaccg gcttaagtta ggtgtgtttt ctacgactag
     2041 tgaatgccct actagaagat gtgtgttgca caaaatgtcc ctggaataac caatttgaag
     2101 tgcagatagc agtaaacgta agctaatatg aatattattt aactgtaatg ttttaatatc
     2161 gctggacatt actaataaac ccactataaa cacatgtaca tatgtatgtt ttggcataca
     2221 atgagtagtt ggggaaaaaa tgtgtaaaag caccgtgacc atcacagcat aaagataacc
     2281 agctgaagta tcgaatatga gtaaccccca aattgaatca catgccgcaa ctgataggac
     2341 ccatggaagt acactcttca tggcgatata caagacacac acaagcacga acacccagtt
     2401 gcggaggaaa ttctccgtaa atgaaaaccc aatcggcgaa caattcatac ccatatatgg
     2461 taaaagtttt gaacgcgact tgagagcgga gagcattgcg gctgataagg ttttagcgct
     2521 aagcgggctt tataaaacgg gctgcgggac cagttttcat atcactaccg tttgagttct
     2581 tgtgctgtgt ggatactcct cccgacacaa agccgctcca tcagccagca gtcgtctaat
     2641 ccagagaccc cggatcgggg taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc
     2701 gctgagaaac aagctcaatg gaaagctgca aacggtggat ctcctccaca tatggcttac
     2761 ccatacgatg ttccagatta cgctcctcca agcttgaatt ctatgccact gctgaactcg
     2821 acgcagttcg tccaggcctt cacgtacctt atccagaacg ataaggagtt tgccaacaag
     2881 ctgcacaagg cctacctgaa cggctgttcc aatctactgc tggactccag ctccacatat
     2941 caagaattca tgtcccctat actaggttat tggaaaatta agggccttgt gcaacccact
     3001 cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa
     3061 ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa tcttccttat
     3121 tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta tatagctgac
     3181 aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga
     3241 gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga ctttgaaact
     3301 ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga agatcgttta
     3361 tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat gttgtatgac
     3421 gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc aaaattagtt
     3481 tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa atccagcaag
     3541 tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga ccatcctcca
     3601 aaatcggtag gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     3661 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
     3721 taaacccgct gatcagcctc gactgtgcct tctaaggcct gagctcgctg atcagcctcg
     3781 atcgaggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     3841 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     3901 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     3961 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     4021 tatgatcagt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     4081 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     4141 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     4201 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     4261 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     4321 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     4381 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     4441 gcaggtagat gacgaccatc agggacagct tcaaggccag caaaaggcca ggaaccgtaa
     4501 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4561 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4621 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4681 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4741 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4801 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4861 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4921 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     4981 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5041 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5101 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5161 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5221 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5281 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5341 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5401 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5461 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5521 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5581 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5641 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5701 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5761 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5821 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5881 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     5941 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6001 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6061 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6121 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6181 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6241 tccgcgcaca tttcccc
//
