LOCUS       sequence-396938-        4380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF680 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF680
FEATURES             Location/Qualifiers
     source          1..4380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1691..1790)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1807..1826)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1808..1826)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1831..1847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1831..1847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2278..3069
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3162..3750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3651..3670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3904..3921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4080..4107)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4199..4285)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4334..4356
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4348..4365
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4349..4365
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgccaggacc acctggaagc
      121 ctagaaatgg gaccactgac atttagggat gtggccatag aattctctct ggaggagtgg
      181 caatgcctgg acactgcaca acggaattta tataggaaag tgatgtttga gaactacaga
      241 aacctggtct tcctgggtat tgctgtctct aagcctcacc tgataacctg tttggagcaa
      301 ggaaaagagc cctggaatag gaagagacag gagatggtag ccaaaccccc agttatatat
      361 tctcatttca ctgaagacct ttggccagag catagcataa aagattcttt tcaaaaagtg
      421 atactgagag gatatggaaa atgtggacat gagaatttac aattaagaat aagttgtaaa
      481 agtgtggatg agtctaaggt gttcaaagaa ggttataatg aacttaacca atgtttgaga
      541 actacccaga gcaaaatatt tcaatgtgat aaatacgtga aagtctttca taaattttca
      601 aattcaaaca gtcataagaa aagaaatact ggaaagaagg ttttcaaatg taaagaatgt
      661 ggcaaatcat tttgcatgct ttcacatcta acacaacata taagaattca cactagagag
      721 aattcttaca aatgtgagga atgtggcaaa gttcttaact ggttctcaga gcttattaaa
      781 cataagggaa ttcatatggg agagaaaccc tacaaatgtg aggaatgtgg caaagccttt
      841 aaccaatcct caacccttat taaacataag aaaattcata ttgaagagaa acccttcaaa
      901 tgtgaagaat gtggcaaagc ctttagttta ttctcaatcc ttagtaaaca taagataatt
      961 catactggag acaaacctta caaatgtgat gaatgtcaca aagcctttaa ctggtttgca
     1021 acccttacta accataagag aattcatact ggagagaaac ccttcaaatg tgaagaatgt
     1081 ggcaaaggct ttaaccagtt ttcaaacctt actaaacata agaaaattca tactggagag
     1141 aaaccctaca aatgtgaaga atgtggcaaa gcttttaacc agtttgcaaa ccttactaga
     1201 cataagaaaa ttcatactgg agagaaatcc tacaaatgtg aagaatgcgg caaagctttt
     1261 atacagtcct caaaccttac tgaacatatg agaattcata ctggagagaa accctacaaa
     1321 tgtgaagaat gtggcaaagc ttttaatggg tgctccagcc ttactcgaca taagagaatt
     1381 cacactagag agaataccta caaatgtgaa gaatgtggca aaggctttac tttattttca
     1441 acccttacta accataaagt aattcatact ggagagaaat cctacaaatg tgatgaatgt
     1501 ggcaatgttt ttaactggcc tgcaactctt gctaatcata agagaattca tgctagagag
     1561 aaaccctaca aatgtgaaga atgtggcaaa gcttttaacc ggtcctcaca ccttactaga
     1621 cataagaaaa ttcatactgg tgagaaactc tacaaacctg aaaaatgcga cgataatttt
     1681 gataacacct acccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1741 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1801 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1861 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     1921 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     1981 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     2041 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     2101 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     2161 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     2221 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
     2281 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     2341 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     2401 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     2461 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     2521 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     2581 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     2641 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     2701 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     2761 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2821 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2881 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     2941 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     3001 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     3061 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     3121 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3181 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3241 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3301 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3361 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3421 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3481 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3541 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3601 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3661 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3721 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3781 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3841 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3901 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3961 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4021 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     4081 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     4141 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     4201 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4261 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     4321 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
