LOCUS       sequence-396939-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for AVK neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH54 - [AVKp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1900..1916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1900..1916)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1913..1935)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2088..2676)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2168..2187)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2793..2817)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2917..3708,3709..3777))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3540..3559
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3778..3882)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3981..4003
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3995..4012
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3996..4012
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4636..4663,1..6))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agttatgata tcaaaaatga ttacgccaag ctgtaagttt aaacatgatc ttactaacta
       61 actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac aacgatggat
      121 acgctaatgc cgtgaatatt tttagactgg aataagttat attattttga ccattagtgt
      181 atttattgtt agaccctact gaggaatact tttcatattg cttttattga ttaaataacc
      241 tatttttaac tcttctcatc caaatcagtt gctgcctaaa caaaacaaat tgaacacaat
      301 aacttaaaca aatatttcta aactaataca attaccaaat tattctggtt tcataatttc
      361 catatgggac tcattgaatg ctacgctttt catcattttt acacatttct atccgaatac
      421 tatacgcaaa aagagaccaa aaatggggcc ctctaagggc gaagaattgt tcacgggagt
      481 agtcccaatc ttagtcgagc ttgacggagg tgagttttat tatcgatttt cgggattttc
      541 tacttgtaat taccttttgt ttttaataag ttgctttttt ccagatgtta atggccacaa
      601 gttttccgtg tctggagaag gagaaggcga cgctacctac ggtaagctga ccctgaagtt
      661 catttgcact actggcaagt taccagtacc atggccgacc ctggtgacaa cattttgcta
      721 cggggttcaa tgcttctcca gatacccagg taggttttca gtttcaattt ttgttgcaac
      781 tacctcaact ctatgttttc agatcatatg aaaagacacg acttttttaa atccgccatg
      841 ccagagggtt acgttcaaga acgtaccatt ttttttaagg acgatggtaa ctacaagaca
      901 cgtgcggagg ttaaatttga aggcgatact ctcgtgaacc gtattgagct caaggtaaat
      961 ttttcaagct tctaaattcc aaaataaacc attttaattt cttaattcca gggaatcgat
     1021 ttcaaggaag atggaaacat tttgggccac aagcttgaat ataattataa tagtcataat
     1081 gtctacatta tggccgacaa acaaaaaaac ggtattaagg ttaacttcaa gattcgccac
     1141 aatatcgaag atggatctgt ccaacttgct gaccactacc aacaaaatac tccaattgga
     1201 gatggaccgg ttttgcttcc agataaccac tacctttcca ctcagtctgc cctctctaag
     1261 gatccgaatg aaaaacgtga ccatatggtt cttttggagt tcgttaccgc agcaggaatt
     1321 actcacggaa tggatgaact gtacaagccc gggtgatggt ctctgagcta tgcaaaatcc
     1381 tttcaagcat tcccttcttc tctatcactc ttctttcttt ttgtcaaaaa attctctcgc
     1441 taatttattt gcttttttaa tgttattatt ttatgacttt ttatagtcac tgaaaagttt
     1501 gcatctgagt gaagtgaatg ctatcaaaat gtgattctga tatccaggaa acggagaatc
     1561 tgtgcggcaa tgtcttatca tttgaaacgc gccgtatcga tgctgacatc atgcggctcc
     1621 cgtgttagtc gagaagatgg gtacgggcct ttattcatcc aacactctac ggcttcttca
     1681 agagctagta gggcaccctg tagttggaaa gggattattt cgtatggcga gcccataccg
     1741 tctttcttgc ggaagcttaa cacgatagga agttggatag tttcgaacga tggttccata
     1801 cggaacgctg tctcaaggat gagtctgacg gagtgtaact cgaaggctag gtggaggctc
     1861 agtgatgata agtctgcgat ggtggatgca tgtgtcatgg tcatagctgt ttcctgtgtg
     1921 aaattgttat ccgctcagag ggcacaatcc tattccgcgc tatccgacaa tctccaagac
     1981 attaggtgga gttcagttcg gcgtatggca tatgtcgctg gaaagaacat gtgagcaaaa
     2041 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2101 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2161 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2221 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2281 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2341 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2401 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2461 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2521 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2581 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2641 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2701 gggtctgacg ctctattcaa caaagccgcc gtcccgtcaa gtcagcgtaa atgggtaggg
     2761 ggcttcaaat cgtcctcgtg ataccaattc ggagcctgct tttttgtaca aacttgttga
     2821 taatggcaat tcaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     2881 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     2941 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     3001 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3061 gccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3121 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3181 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3241 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3301 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3361 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3421 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3481 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3541 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3601 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3661 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3721 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3781 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3841 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     3901 gccagatacc tgaaacaaaa cccatcgtac ggccaaggaa gtctccaata actgtgatcc
     3961 accacaagcg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtcatgcata
     4021 atccgcacgc atctggaata aggaagtgcc attccgcctg acctaagtgc ttttcgagat
     4081 cgccaaaccg gagaacgatc aggagaatga tgagtcgcca caggagtcgg caagatacgt
     4141 gcgttataga tttgctccaa actttttgaa actcaaaacg gtcggagcaa ctgtggaatt
     4201 caaagtagga gacatcccaa tccatcattt ccgaatgatc gaacgtcact tcttcaaaga
     4261 tcgccttctg aagtgttttg actttgaatt cggattctgt attccgaatt cacgaaacaa
     4321 ctgtgaacat atctatgagt tccctcaact ctctcaacaa ctcagtgagt cattattcta
     4381 aaaagtacaa ttcaaaagac taatctcttt ttcagtggac gacatgatca acaatccaaa
     4441 cgagactcgt tctgacagct tctatttcgt cgataacaaa ctcgtcatgc acaacaaagc
     4501 cgactactca tatgatgcat aaatcttcat tgttgagttt atcttgttga tttttgaata
     4561 aattatcaac tctttacttt ttaatgggtt atgaaataaa taaacattga aaactgataa
     4621 acaacgttca tctctataac ttcgtatagc atacattata cga
//
