LOCUS       sequence-396941-        4263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF655 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF655
FEATURES             Location/Qualifiers
     source          1..4263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1574..1673)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1690..1709)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1691..1709)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1714..1730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1714..1730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2161..2952
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3045..3633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3534..3553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3787..3804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3963..3990)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4082..4168)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4217..4239
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4231..4248
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4232..4248
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggaggaaat accagcccag
      121 gaagcagcag ggtcaccaag ggtccagttt cagtctttgg agacccagtc tgagtgtctg
      181 tccccagagc ctcagtttgt gcaggacacc gacatggaac agggactcac tggggatgga
      241 gagaccagag aagataacaa gctgttgatt cctaagcaga aaatttcgga agaagtgcat
      301 tcatacaaag tgagagtagg aagactcaaa cacgatatta cccaagttcc tgagactaga
      361 gaagtgtata agtctgagga cagattagaa agacttcagg aaattctaag gaaatttctg
      421 tacctggaga gagagtttag gcaaataaca atcagcaagg aaaccttcac cagtgagaag
      481 aacaatgaat gtcatgaacc cgaaaaaagc ttcagtctgg actctactat tgatgcagat
      541 cagagagttc ttagaataca gaataccgat gacaatgata agtatgacat gagcttcaac
      601 cagaattcag cctctggtaa acatgaacac ttaaatctaa cagaggattt tcagagtagt
      661 gaatgtaagg aaagcttaat ggatctctcc caccttaata aatgggagag catccctaac
      721 actgagaaat cctataaatg tgatgtatgt gggaaaattt tccatcagag ctcagccctt
      781 actagacatc agagaatcca tactagagag aagccctaca aatgtaaaga atgtgaaaag
      841 tctttcagtc agagctcaag tcttagtcga cataaaagaa tacacactag agaaaaacct
      901 tacaaatgtg aagcatctga taaatcctgt gaagcgtctg ataaatcctg tagtccaagc
      961 tcaggcataa ttcagcataa gaaaattcac accagagcca aatcttacaa atgtagcagt
     1021 tgtgaaagag tcttcagtcg tagtgtccac cttactcaac atcagaaaat tcacaaagag
     1081 atgccctgta agtgtactgt atgtggcagt gacttctgcc atacttcata cctacttgaa
     1141 catcagaggg tccatcatga agagaaagcc tatgagtatg atgaatatgg gttggcctat
     1201 attaaacaac aaggaattca tttcagagaa aagccctata cgtgtagtga atgtggaaaa
     1261 gacttcagat tgaattcaca tcttattcag catcaaagaa ttcacacagg agagaaagca
     1321 catgaatgta atgaatgtgg aaaagctttc agtcaaacct catgccttat tcagcatcac
     1381 aaaatgcata ggaaagagaa atcgtatgaa tgtaatgagt atgagggcag tttcagtcat
     1441 agctcagatc ttatcctgca acaagaagtc ctcaccagac agaaagcctt tgattgtgat
     1501 gtatgggaaa agaactccag tcagagagca catctagttc aacatcagag cattcatacc
     1561 aaagagaact catgcccaac tttcttgtac aaagttggca ttataagaaa gcattgctta
     1621 tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca
     1681 gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     1741 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc
     1801 ctctagacca gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga
     1861 cgcacaccgt ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt
     1921 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     1981 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     2041 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     2101 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt
     2161 atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     2221 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     2281 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     2341 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     2401 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     2461 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     2521 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     2581 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     2641 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     2701 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     2761 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     2821 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     2881 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     2941 gtcggcaaat aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg
     3001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     3721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3781 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     3841 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     3901 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     3961 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     4021 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     4081 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4141 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     4201 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     4261 ccc
//
