LOCUS       sequence-396942-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for ASER neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH57 - [ASERp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1905..1921)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1905..1921)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1918..1940)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2093..2681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2173..2192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2798..2822)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2922..3713,3714..3782))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3545..3564
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3783..3887)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3986..4008
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4000..4017
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4001..4017
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4641..4645,1..29))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttcgtatagc atacattata cgaagttatg atatcaaaaa tgattacgcc aagctgtaag
       61 tttaaacatg atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc
      121 tgaaatcact cacaacgatg gatacgctaa tgccttcatt attaagcaaa ttcaagtata
      181 ataacaacta taaataagac caccaaattt tgaaatccgg attaaaatgt tatttttgaa
      241 tagagaagtt tgatgcagat accaacaaga ttaaaacttc aaacattaag caagaagccc
      301 ccaaatgcgt atggtttcaa agaaaaaggt gcaaaattgg gaagataatc tcgtttaaga
      361 aaagtttgga aatctattaa gaccaacccc cagaagtagt agaatttcaa tttactattt
      421 attgttttcg aaaaattact tattctgatg aaaaagagac caaaaatggg gcccgtttca
      481 aagggagagg ccgttataaa agaatttatg cgtttcaagg tccacatgga gggatccatg
      541 aacggacacg agttcgagat cgagggagag ggagagggac gtccatacga gggaacccaa
      601 accgccaagg tgagttttat tatcgatttt cgggattttc tacttgtaat taccttttgt
      661 ttttaataag ttgctttttt ccagctcaag gtcaccaagg gaggaccact cccattctcc
      721 tgggacatcc tctccccaca attcatgtac ggatcccgtg ccttcaccaa gcacccagcc
      781 gacatcccag actactacaa gcaatccttc ccagagggat tcaagtggga gcgtgtcatg
      841 aacttcgagg acggaggagc cgtcaccgtc acccaaggta ggttttcagt ttcaattttt
      901 gttgcaacta cctcaactct atgttttcag acacctccct cgaggacgga accctcatct
      961 acaaggtcaa gctccgtgga accaacttcc caccagacgg accagtcatg caaaagaaga
     1021 ccatgggatg ggaggcctcc accgagcgac tctacccaga ggacggagtc ctcaagggag
     1081 acatcaaggt aaatttttca agcttctaaa ttccaaaata aaccatttta atttcttaat
     1141 tccagatggc cctccggctc aaggacggag gacgttacct cgccgacttc aagaccacct
     1201 acaaggccaa gaagccagtc caaatgccag gagcctacaa cgtcgaccgt aagctcgaca
     1261 tcacctccca caacgaggac tacaccgtcg tcgagcaata cgagcgttcc gagggacgtc
     1321 actccaccgg aggaatggac gagctctaca agcccgggtg atggtctctg agctatgcaa
     1381 aatcctttca agcattccct tcttctctat cactcttctt tctttttgtc aaaaaattct
     1441 ctcgctaatt tatttgcttt tttaatgtta ttattttatg actttttata gtcactgaaa
     1501 agtttgcatc tgagtgaagt gaatgctatc aaaatgtgat tctgatatcc aggaaacgga
     1561 gaatctgtgc ggcaatgtct tatcatttga aacgcgccgt atcgatgctg acatcatgcg
     1621 gctcccgtgt tagtcgagaa gatgggtacg ggcctttatt catccaacac tctacggctt
     1681 cttcaagagc tagtagggca ccctgtagtt ggaaagggat tatttcgtat ggcgagccca
     1741 taccgtcttt cttgcggaag cttaacacga taggaagttg gatagtttcg aacgatggtt
     1801 ccatacggaa cgctgtctca aggatgagtc tgacggagtg taactcgaag gctaggtgga
     1861 ggctcagtga tgataagtct gcgatggtgg atgcatgtgt catggtcata gctgtttcct
     1921 gtgtgaaatt gttatccgct cagagggcac aatcctattc cgcgctatcc gacaatctcc
     1981 aagacattag gtggagttca gttcggcgta tggcatatgt cgctggaaag aacatgtgag
     2041 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2101 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2161 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2221 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2281 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2341 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2401 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2461 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2521 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2581 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2641 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2701 ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag cgtaaatggg
     2761 tagggggctt caaatcgtcc tcgtgatacc aattcggagc ctgctttttt gtacaaactt
     2821 gttgataatg gcaattcaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2881 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2941 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3001 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3061 cgagagccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3121 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3181 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3241 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     3301 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     3361 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3421 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3481 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3541 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3601 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3661 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3721 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3781 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3841 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3901 aaagtgccag atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     3961 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     4021 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgaccta agtgcttttc
     4081 gagatcgcca aaccggagaa cgatcaggag aatgatgagt cgccacagga gtcggcaaga
     4141 tacgtgcgtt atagatttgc tccaaacttt ttgaaactca aaacggtcgg agcaactgtg
     4201 gaattcaaag taggagacat cccaatccat catttccgaa tgatcgaacg tcacttcttc
     4261 aaagatcgcc ttctgaagtg ttttgacttt gaattcggat tctgtattcc gaattcacga
     4321 aacaactgtg aacatatcta tgagttccct caactctctc aacaactcag tgagtcatta
     4381 ttctaaaaag tacaattcaa aagactaatc tctttttcag tggacgacat gatcaacaat
     4441 ccaaacgaga ctcgttctga cagcttctat ttcgtcgata acaaactcgt catgcacaac
     4501 aaagccgact actcatatga tgcataaatc ttcattgttg agtttatctt gttgattttt
     4561 gaataaatta tcaactcttt actttttaat gggttatgaa ataaataaac attgaaaact
     4621 gataaacaac gttcatctct ataac
//
