LOCUS       sequence-396943-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for ASER neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH58 - [ASERp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1910..1926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1910..1926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1923..1945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2098..2686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2178..2197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2803..2827)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2927..3718,3719..3787))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3550..3569
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3788..3892)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3991..4013
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4005..4022
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4006..4022
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4646..4663,1..16))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cattatacga agttatgata tcaaaaatga ttacgccaag ctgtaagttt aaacatgatc
       61 ttactaacta actattctca tttaaatttt cagagcttaa aaatggctga aatcactcac
      121 aacgatggat acgctaatgc cttcattatt aagcaaattc aagtataata acaactataa
      181 ataagaccac caaattttga aatccggatt aaaatgttat ttttgaatag agaagtttga
      241 tgcagatacc aacaagatta aaacttcaaa cattaagcaa gaagccccca aatgcgtatg
      301 gtttcaaaga aaaaggtgca aaattgggaa gataatctcg tttaagaaaa gtttggaaat
      361 ctattaagac caacccccag aagtagtaga atttcaattt actatttatt gttttcgaaa
      421 aattacttat tctgatgaaa aagagaccaa aaatggggcc ctctaagggc gaagaattgt
      481 tcacgggagt agtcccaatc ttagtcgagc ttgacggagg tgagttttat tatcgatttt
      541 cgggattttc tacttgtaat taccttttgt ttttaataag ttgctttttt ccagatgtta
      601 atggccacaa gttttccgtg tctggagaag gagaaggcga cgctacctac ggtaagctga
      661 ccctgaagtt catttgcact actggcaagt taccagtacc atggccgacc ctggtgacaa
      721 cattttgcta cggggttcaa tgcttctcca gatacccagg taggttttca gtttcaattt
      781 ttgttgcaac tacctcaact ctatgttttc agatcatatg aaaagacacg acttttttaa
      841 atccgccatg ccagagggtt acgttcaaga acgtaccatt ttttttaagg acgatggtaa
      901 ctacaagaca cgtgcggagg ttaaatttga aggcgatact ctcgtgaacc gtattgagct
      961 caaggtaaat ttttcaagct tctaaattcc aaaataaacc attttaattt cttaattcca
     1021 gggaatcgat ttcaaggaag atggaaacat tttgggccac aagcttgaat ataattataa
     1081 tagtcataat gtctacatta tggccgacaa acaaaaaaac ggtattaagg ttaacttcaa
     1141 gattcgccac aatatcgaag atggatctgt ccaacttgct gaccactacc aacaaaatac
     1201 tccaattgga gatggaccgg ttttgcttcc agataaccac tacctttcca ctcagtctgc
     1261 cctctctaag gatccgaatg aaaaacgtga ccatatggtt cttttggagt tcgttaccgc
     1321 agcaggaatt actcacggaa tggatgaact gtacaagccc gggtgatggt ctctgagcta
     1381 tgcaaaatcc tttcaagcat tcccttcttc tctatcactc ttctttcttt ttgtcaaaaa
     1441 attctctcgc taatttattt gcttttttaa tgttattatt ttatgacttt ttatagtcac
     1501 tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat gtgattctga tatccaggaa
     1561 acggagaatc tgtgcggcaa tgtcttatca tttgaaacgc gccgtatcga tgctgacatc
     1621 atgcggctcc cgtgttagtc gagaagatgg gtacgggcct ttattcatcc aacactctac
     1681 ggcttcttca agagctagta gggcaccctg tagttggaaa gggattattt cgtatggcga
     1741 gcccataccg tctttcttgc ggaagcttaa cacgatagga agttggatag tttcgaacga
     1801 tggttccata cggaacgctg tctcaaggat gagtctgacg gagtgtaact cgaaggctag
     1861 gtggaggctc agtgatgata agtctgcgat ggtggatgca tgtgtcatgg tcatagctgt
     1921 ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc tattccgcgc tatccgacaa
     1981 tctccaagac attaggtgga gttcagttcg gcgtatggca tatgtcgctg gaaagaacat
     2041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2521 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2701 cttttctacg gggtctgacg ctctattcaa caaagccgcc gtcccgtcaa gtcagcgtaa
     2761 atgggtaggg ggcttcaaat cgtcctcgtg ataccaattc ggagcctgct tttttgtaca
     2821 aacttgttga taatggcaat tcaaggatct tcacctagat ccttttaaat taaaaatgaa
     2881 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2941 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3001 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3061 taccgcgaga gccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3121 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3181 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3241 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3301 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3361 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3421 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3481 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3541 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3601 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3661 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3721 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3781 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3841 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3901 cccgaaaagt gccagatacc tgaaacaaaa cccatcgtac ggccaaggaa gtctccaata
     3961 actgtgatcc accacaagcg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     4021 gtcatgcata atccgcacgc atctggaata aggaagtgcc attccgcctg acctaagtgc
     4081 ttttcgagat cgccaaaccg gagaacgatc aggagaatga tgagtcgcca caggagtcgg
     4141 caagatacgt gcgttataga tttgctccaa actttttgaa actcaaaacg gtcggagcaa
     4201 ctgtggaatt caaagtagga gacatcccaa tccatcattt ccgaatgatc gaacgtcact
     4261 tcttcaaaga tcgccttctg aagtgttttg actttgaatt cggattctgt attccgaatt
     4321 cacgaaacaa ctgtgaacat atctatgagt tccctcaact ctctcaacaa ctcagtgagt
     4381 cattattcta aaaagtacaa ttcaaaagac taatctcttt ttcagtggac gacatgatca
     4441 acaatccaaa cgagactcgt tctgacagct tctatttcgt cgataacaaa ctcgtcatgc
     4501 acaacaaagc cgactactca tatgatgcat aaatcttcat tgttgagttt atcttgttga
     4561 tttttgaata aattatcaac tctttacttt ttaatgggtt atgaaataaa taaacattga
     4621 aaactgataa acaacgttca tctctataac ttcgtatagc ata
//
