LOCUS       sequence-396952-       10972 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SIGLEC1(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..10972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6548..6592
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6593..6610
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6750..6767)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6756..6867
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6985..7005)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6990..7319
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7170..7305
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7232..7251
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7367..7414
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7433..8032
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7433..7452)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7929..7949
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8162..8283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8199..8218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8253..8272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8336..9127,9128..9196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8959..8978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9197..9288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9330..9349
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9355..9374
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9460..10048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9949..9968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10202..10219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10317..10334
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10318..10334
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10790..10806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg cctcttgggg agttagttct cctcaggatg tgcagggcgt
     1021 gaagggctct tgtctgctga tcccctgcat cttcagcttc cccgctgacg tggaagtgcc
     1081 tgatggaatc accgccatct ggtactacga ctacagcggc cagagacagg tggtgtctca
     1141 ctctgccgat cctaagctgg tggaagccag attcagaggc agaaccgagt tcatgggcaa
     1201 ccccgagcac agagtgtgca acctgctgct gaaagacctg cagcctgagg acagcggcag
     1261 ctacaacttc agattcgaga tcagcgaagt gaaccgttgg agcgacgtga agggaaccct
     1321 ggtcacagtg accgaggaac ccagagtgcc tacaatcgcc tctcctgtgg aactgctgga
     1381 aggcaccgag gtggacttca actgtagcac cccttacgtg tgcctgcaag agcaagttcg
     1441 gctgcagtgg cagggacaag atcctgccag aagcgtgacc ttcaacagcc agaagtttga
     1501 gccaacaggc gtgggccacc tggaaaccct gcatatggct atgtcttggc aggaccacgg
     1561 cagaatcctg agatgccagc tgagcgtggc caatcacaga gcccagagcg agatccatct
     1621 gcaagtgaag tatgccccta aaggcgtgaa gattctgctg agccccagcg gcagaaatat
     1681 cctgcctggc gagctggtca ccctgacctg tcaagtgaat agcagctacc ccgccgtgtc
     1741 cagcatcaag tggctgaagg atggcgtgcg gctgcagaca aaaactggcg ttctgcatct
     1801 gcctcaggcc gcttggagtg atgctggcgt gtacacctgt caggccgaga atggcgttgg
     1861 ctctctggtg tctcctccta tctctctgca catcttcatg gccgaggtcc aggtttcccc
     1921 tgccggacct atcctggaaa accagaccgt gacactcgtg tgcaacaccc ctaatgaggc
     1981 ccctagcgac ctgcggtaca gctggtataa gaaccacgtg ctgctcgagg acgcccactc
     2041 tcacacactg agactgcatc tggccaccag agccgatacc ggcttctact tctgcgaggt
     2101 gcagaatgtg cacggctctg agagatctgg ccctgtgtcc gtggtggtca atcaccctcc
     2161 actgacacct gtgctgaccg cctttctgga aacacaggcc ggactcgtgg gcatcctgca
     2221 ctgttctgtg gtgtctgaac ctctggccac actggtgctg tctcacggcg gacatattct
     2281 ggccagcaca agcggcgata gcgatcacag ccctagattc agcggcacca gcggacctaa
     2341 cagcctgcgg ctggaaatcc gcgacctgga agaaacagac tccggcgagt acaagtgcag
     2401 cgccaccaat tctctgggca acgccaccag cacactggac tttcatgcca atgccgccag
     2461 actgctgatt tctcctgctg ccgaagtggt ggaaggacag gctgtgacac tgtcctgtag
     2521 aagcggactg agccctacac ctgatgccag gtttagctgg tatctgaacg gcgccctgct
     2581 gcatgaaggc cctggatctt ctcttctgct gcctgccgct tctagcaccg atgccggctc
     2641 ttatcactgt agagccagag atggacacag cgcctctgga ccatcttctc cagctgtcct
     2701 gacagtgctg taccctccta gacagcccac cttcaccacc agactggatt tggatgctgc
     2761 tggtgctggc gctgggagaa gaggactgct gctgtgtaga gtggacagcg atcctcctgc
     2821 tagactgcag ctgctgcaca aggatagagt ggtggccaca tctctgccta gcggcggagg
     2881 atgttctacc tgtggcggct gtagccccag aatgaaggtt acaaaggccc ctaacctgct
     2941 gagggtcgag atccacaatc ctctgctcga agaggaaggc ctgtacctct gcgaagccag
     3001 caatgccctg ggcaatgcca gcacctctgc cacctttaat ggacaggcca ccgtgctggc
     3061 tatcgcccct agtcatacac tgcaagaggg cacagaggcc aacctgacct gcaatgtgtc
     3121 aagagaagcc gccggatctc ccgccaactt cagctggttt agaaacggcg tgctgtgggc
     3181 ccagggacct cttgaaacag tgacactgct gcccgtggcc agaacagatg ccgcactgta
     3241 tgcctgtcgg attctgacag aagctggcgc ccagctgtct acacctgttc tgctgtctgt
     3301 gctgtatcca cctgacagac ctaagctgtc tgccctgctg gatatgggac agggacacat
     3361 ggccctgttc atctgcaccg tggatagcag accactggct ctgcttgccc tgtttcatgg
     3421 cgaacatctg ctggccacaa gcctgggacc tcaggtgcca tctcatggca gatttcaggc
     3481 caaggccgag gccaatagcc tgaagctgga agtccgagaa ctcggcctgg gcgattctgg
     3541 cagctataga tgcgaggcca caaacgtgct gggcagcagc aacaccagcc tgttctttca
     3601 agtccgcgga gcctgggtgc aagtgtctcc atctcctgaa ctccaagagg gccaagccgt
     3661 cgtgctgtct tgtcaagtgc atacaggcgt gccagagggc accagctaca gatggtatag
     3721 agatggccag cctctgcaag agtccaccag cgctacactg agattcgccg ccatcacact
     3781 tacccaggct ggcgcatatc attgccaggc tcaagcccca ggctctgcca caacaagtct
     3841 ggctgctcct atcagcctgc acgtgtcata tgccccaaga cacgtgaccc tgaccacact
     3901 gatggataca ggacctggca gactgggcct gctcctgtgc agagttgatt ccgatcctcc
     3961 agcacagctg cgactgctcc atggcgatag actggttgcc tctactctgc aaggcgttgg
     4021 cggacctgaa ggcagctctc caagactgca tgtcgccgtg gctcccaaca ctctgagact
     4081 cgaaattcac ggggccatgc tggaagatga aggggtgtac atttgcgagg cctctaatac
     4141 cctgggacaa gcctctgcca gcgccgactt tgatgcacag gcagtgaatg tccaagtgtg
     4201 gcctggcgcc acagtcagag aaggccagct ggtcaatctg acatgcctcg tgtggacaac
     4261 acaccccgca cagctgacct acacatggta tcaggacgga cagcagagac tggacgccca
     4321 tagcatccct ctgcctaatg tgactgtgcg ggacgccacc tcttacagat gcggagttgg
     4381 acctcctgga cgggctccta gactgagcag acctatcact ctggacgtgc tgtacgcccc
     4441 tcggaacctg agactgacat acctgcttga gtctcatggc ggccaactgg ccctggtgct
     4501 ctgtactgtt gatagcaggc ctcctgctca gctcgctctg tctcatgctg ggagactgct
     4561 ggcctcttct accgccgctt ctgtgcctaa tactctgagg ctggaactga gaggccctca
     4621 gcctagagat gagggctttt acagctgcag cgctagatct ccactcggcc aggctaatac
     4681 ctctctcgag ctgagactgg aaggcgtcag agtgattctg gctcctgaag cagctgttcc
     4741 cgaaggcgcc cctattaccg tgacatgtgc tgatcctgcc gctcacgccc ctacactgta
     4801 tacctggtat cacaacggcc ggtggctgca agaaggacct gctgcctctc tgagcttcct
     4861 ggtggctaca agagcacacg ctggcgccta tagctgtcag gcacaagatg cccagggaac
     4921 cagatctagt agaccagccg ctctgcaggt tctgtacgct ccacaggatg ccgtgctgag
     4981 cagcttcaga gactccagag ccagatctat ggccgtgatc cagtgtaccg tggattctga
     5041 gcctcctgcc gaactggcac tgagccacga tggaaaagtg ctggcaacat ctagcggcgt
     5101 gcacagcctt gcctctggaa caggacatgt gcaggtcgcc agaaacgccc tgagacttca
     5161 ggtgcaagat gtgcctgccg gcgacgatac ctatgtgtgt acagcccaga atctgctggg
     5221 ctccatcagc acaatcggca gactgcaggt tgagggcgcc agagttgttg ccgaacctgg
     5281 acttgatgtg ccagaaggcg ccgctctgaa cctgtcttgt agactgcttg gaggccccgg
     5341 acctgtgggc aatagcacat tcgcctggtt ctggaacgac agaaggctgc acgctgagcc
     5401 tgtgcctaca ctggccttta cacatgtggc tagagcccag gcaggcatgt atcactgtct
     5461 ggctgaactg cctacaggcg ctgctgcttc tgcccctgtt atgctgagag tgctctaccc
     5521 accaaagact cccaccatga tggtgttcgt tgagcctgaa ggcgggctga gaggcatcct
     5581 ggattgcaga gtggactccg agccactggc aagcctgaca cttcatctgg gaagcagact
     5641 ggtggcatct agtcagcctc aaggcgctcc agccgaacct cacattcacg tcctggcctc
     5701 tccaaacgca ctgagagtgg atattgaggc cctgaggcct tccgaccagg gcgagtatat
     5761 ctgtagcgcc tccaatgtgc tgggaagcgc ctctaccagc acatactttg gcgtcagggc
     5821 actgcacaga ctgcaccagt ttcagcaagg cgcgccgggc ggcgacaaga cccacacctg
     5881 ccccccctgc ccagctccag aactgctggg agggccttcc gtgttcctgt tcccccccaa
     5941 gcccaaggac accctgatga tcagcaggac ccccgaggtg acctgcgtgg tggtggccgt
     6001 gtcccacgag gacccagagg tgaagttcaa ctggtacgtg gacggcgtgg aggtgcacaa
     6061 cgccaagacc aagcccagag aggagcagta caacagcacc tacagggtgg tgtccgtgct
     6121 gaccgtgctg caccaggact ggctgaacgg caaagaatac aagtgcaaag tctccaacaa
     6181 ggccctggct gccccaatcg aaaagacaat cagcaaggcc aagggccagc cacgggagcc
     6241 ccaggtgtac accctgcccc ccagccggga ggagatgacc aagaaccagg tgtccctgac
     6301 ctgtctggtg aagggcttct accccagcga tatcgccgtg gagtgggaga gcaacggcca
     6361 gcccgagaac aactacaaga ccaccccccc agtgctggac agcgacggca gcttcttcct
     6421 gtacagcaag ctgaccgtgg acaagtccag gtggcagcag ggcaacgtgt tcagctgcag
     6481 cgtgatgcac gaggccctgc acaaccacta cacccagaag tccctgagcc tgagccccgg
     6541 cggcagcggt cttaatgata ttttcgaggc tcagaagatt gagtggcatg aacaccacca
     6601 ccatcaccac taaggccggc ctgtagtcac tggcacaacg cgttacccgg taagccaatc
     6661 gggtatacac ggtcgtcata ctgcagacag ggttcttcta ctttgcaaga tagtcttgag
     6721 tagtaaaata aatagataga gaaaatcagc ctcgactgtg ccttctagtt gccagccatc
     6781 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6841 ttcctaataa aatgaggaaa ttgcatcaca acactcaacc ctatctcggt ctattctttt
     6901 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6961 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     7021 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     7081 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7141 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7201 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     7261 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7321 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     7381 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatgaccga
     7441 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     7501 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     7561 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     7621 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     7681 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     7741 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     7801 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     7861 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacatc
     7921 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     7981 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacacgtgct
     8041 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     8101 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     8161 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8221 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8281 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcattacc
     8341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagcg
     8461 ctgcgatgat accgcgagaa ccacgctcac cggctccgga tttatcagca ataaaccagc
     8521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8641 ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8701 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8761 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8821 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8881 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8941 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9001 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9061 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9121 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9181 aatgttgaat actcatattc ttcctttttc aatattattg aagcatttat cagggttatt
     9241 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggtcagtg
     9301 ttacaaccaa ttaaccaatt ctgaacatta tcgcgagccc atttatacct gaatatggct
     9361 cataacaccc cttgctcatg accaaaatcc cttaacgtga gttacgcgcg cgtcgttcca
     9421 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9481 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9541 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9601 tactgttctt ctagtgtagc cgtagttagc ccaccacttc aagaactctg tagcaccgcc
     9661 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9721 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9781 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9841 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9901 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9961 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10021 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10081 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    10141 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    10201 cagcgagtca gtgagcgagg aagcggaagg cgagagtagg gaactgccag gcatcaaact
    10261 aagcagaagg cccctgacgg atggcctttt tgcgtttcta caaactcttt ctgtgttgta
    10321 aaacgacggc cagtcttaag ctcgggcccc ctgggcggtt ctgataacga gtaatcgtta
    10381 atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa
    10441 agagcatttg tcagaatatt taagggcgcc tgtcactttg cttgatatat gagaattatt
    10501 taaccttata aatgagaaaa aagcaacgca ctttaaataa gatacgttgc tttttcgatt
    10561 gatgaacacc tataattaaa ctattcatct attatttatg attttttgta tatacaatat
    10621 ttctagtttg ttaaagagaa ttaagaaaat aaatctcgaa aataataaag ggaaaatcag
    10681 tttttgatat caaaattata catgtcaacg ataatacaaa atataataca aactataaga
    10741 tgttatcagt atttattatc atttagaata aattttgtgt cgcccttaat tgtgagcgga
    10801 taacaattac gagcttcatg cacagtggcg ttgactcaat attggccatt agccatatta
    10861 ttcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
    10921 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg tt
//
