LOCUS       sequence-396953-        6948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For SECRETS protocol to screen for gRNA activity and specificity: 
            bacterial expression Cas9 in the presence of anhydrotetracycline 
            (aTc)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Herring-Nicholas A, Dimig H, Roesing MR, Josephs EA
  TITLE     Selection of extended CRISPR RNAs with enhanced targeting and 
            specificity.
  JOURNAL   Commun Biol. 2024 Jan 12;7(1):86. doi: 10.1038/s42003-024-05776-8.
  PUBMED    38212640
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2024-01-12"; volume: "7(1):86. doi"; pages: " 
            10.1038/s42003-024-05776-8"
COMMENT     Sequence Label: pSECRETS-A
FEATURES             Location/Qualifiers
     source          1..6948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(131..151)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(161..187)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(161..187)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      190..199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(216..241,242..247,248..264,265..270,
                     271..289))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    246..264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    271..289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        366..470
                     /gene="bla"
                     /label=AmpR promoter
     CDS             471..1094
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(544..563)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        1124..1226
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1227..1886
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1293..1312)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1907..2001
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2115..2660
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2561..2580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2777..2794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2822..2849)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(2865..2936)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(2960..6948,1..118))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(join(2963..6948,1..118))
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 ggtctgtatt tcccagaacc ttgaactttt tagacggaac cttatattca tcagtgatca
       61 ccgcccatcc gacgctattt gtgccgatat ctaagcctat tgagtatttc ttatccatgg
      121 ttcggatccc tacctttctc ttcttttttg gaggcaattc ggcgtagtcg ggcacgtcgt
      181 aggggtacac catggtggca agcttgggtc tccctggtca gtgcgtcctg ctgatgtgct
      241 cagtatctct atcactgata gggatgtcaa tctctatcac tgatagggaa atttcgataa
      301 gccagtaagc agtgggttct ctagttagcc agaggacgtc aggtggcact tttcggggaa
      361 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      421 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgtctcgtt
      481 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
      541 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
      601 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
      661 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
      721 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
      781 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
      841 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
      901 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
      961 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     1021 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     1081 aaagtgggtc ttaagacgtc gatatctggc gaaaatgaga cgttgatcgg cacgtaagag
     1141 gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga
     1201 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     1261 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     1321 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     1381 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     1441 aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     1501 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     1561 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     1621 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     1681 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     1741 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     1801 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     1861 gcgatgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact cctgttgata
     1921 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     1981 gcgtttttta ttggtgagaa tccaagcact agtaacaact tatatcgtat ggggctgact
     2041 tcaggtgcta catttgaaga gataaattgc actgaaatct agaaatattt tatctgatta
     2101 ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa
     2161 aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc tttgaaccga
     2221 ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta gccttaaccg
     2281 gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccagtggtgc
     2341 ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2401 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact
     2461 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
     2521 gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc tggtatcttt
     2581 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
     2641 gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat
     2701 cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag
     2761 tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc ctaggtataa
     2821 acgcagaaag gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac
     2881 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
     2941 ctggagatcc ttactcgagt tagtcacctc ctagctgact caaatcaatg cgtgtttcat
     3001 aaagaccagt gatggattga tggataagag tggcatctaa aacttctttt gtagacgtat
     3061 atcgtttacg atcaattgtt gtatcaaaat atttaaaagc agcgggagct ccaagattcg
     3121 tcaacgtaaa taaatgaata atattttctg cttgttcacg tattggtttg tctctatgtt
     3181 tgttatatgc actaagaact ttatctaaat tggcatctgc taaaataaca cgcttagaaa
     3241 attcactgat ttgctcaata atctcatcta aataatgctt atgctgctcc acaaacaatt
     3301 gtttttgttc gttatcttct ggactaccct tcaacttttc ataatgacta gctaaatata
     3361 aaaaattcac atatttgctt ggcagagcca gctcatttcc tttttgtaat tctccggcac
     3421 tagccagcat ccgtttacga ccgttttcta actcaaaaag actatattta ggtagtttaa
     3481 tgattaagtc ttttttaact tccttatatc ctttagcttc taaaaagtca atcggatttt
     3541 tttcaaagga acttctttcc ataattgtga tccctagtaa ctctttaacg gattttaact
     3601 tcttcgattt ccctttttcc accttagcaa ccactaggac tgaataagct accgttggac
     3661 tatcaaaacc accatatttt tttggatccc agtctttttt acgagcaata agcttgtccg
     3721 aatttctttt tggtaaaatt gactccttgg agaatccgcc tgtctgtact tctgttttct
     3781 tgacaatatt gacttggggc atggacaata ctttgcgcac tgtggcaaaa tctcgccctt
     3841 tatcccagac aatttctcca gtttccccat tagtttcgat tagagggcgt ttgcgaatct
     3901 ctccatttgc aagtgtaatt tctgttttga agaagttcat gatattagag taaaagaaat
     3961 attttgcggt tgctttgcct atttcttgct cagacttagc aatcatttta cgaacatcat
     4021 aaactttata atcaccatag acaaactccg attcaagttt tggatatttc ttaatcaaag
     4081 cagttccaac gacggcattt agatacgcat catgggcatg atggtaattg ttaatctcac
     4141 gtactttata gaattggaaa tcttttcgga agtcagaaac taatttagat tttaaggtaa
     4201 tcactttaac ctctcgaata agtttatcat tttcatcgta tttagtattc atgcgactat
     4261 ccaaaatttg tgccacatgc ttagtgattt ggcgagtttc aaccaattgg cgtttgataa
     4321 aaccagcttt atcaagttca ctcaaacctc cacgttcagc tttcgttaaa ttatcaaact
     4381 tacgttgagt gattaacttg gcgtttagaa gttgtctcca atagtttttc atctttttga
     4441 ctacttcttc acttggaacg ttatccgatt taccacgatt tttatcagaa cgcgttaaga
     4501 ccttattgtc tattgaatcg tctttaagga aactttgtgg aacaatgtga tcgacatcat
     4561 aatcacttaa acgattaata tctaattctt ggtccacata catgtctctt ccattttgga
     4621 gataatagag atagagcttt tcattttgca attgagtatt ttcaacagga tgctctttaa
     4681 gaatctgact tcctaattct ttgatacctt cttcgattcg tttcatacgc tctcgcgaat
     4741 ttttctggcc cttttgagtt gtctgatttt cacgtgccat ttcaataacg atattttctg
     4801 gcttatgccg ccccattact ttgaccaatt catcaacaac ttttacagtc tgtaaaatac
     4861 cttttttaat agcagggcta ccagctaaat ttgcaatatg ttcatgtaaa ctatcgcctt
     4921 gtccagacac ttgtgctttt tgaatgtctt ctttaaatgt caaactatca tcatggatca
     4981 gctgcataaa attgcgattg gcaaaaccat ctgatttcaa aaaatctaat attgttttgc
     5041 cagattgctt atccctaata ccattaatca attttcgaga caaacgtccc caaccagtat
     5101 aacggcgacg tttaagctgt ttcatcacct tatcatcaaa gaggtgagca tatgttttaa
     5161 gtctttcctc aatcatctcc ctatcttcaa ataaggtcaa tgttaaaaca atatcctcta
     5221 agatatcttc attttcttca ttatccaaaa aatctttatc tttaataatt tttagcaaat
     5281 catggtaggt acctaatgaa gcattaaatc tatcttcaac tcctgaaatt tcaacactat
     5341 caaaacattc tatttttttg aaataatctt cttttaattg cttaacggtt acttttcgat
     5401 ttgttttgaa gagtaaatca acaatggctt tcttctgttc acctgaaaga aatgctggtt
     5461 ttcgcattcc ttcagtaaca tatttgacct ttgtcaattc gttataaacc gtaaaatact
     5521 cataaagcaa actatgtttt ggtagtactt tttcatttgg aagattttta tcaaagtttg
     5581 tcatgcgttc aataaatgat tgagctgaag cacctttatc gacaacttct tcaaaattcc
     5641 atggggtaat tgtttcttca gacttccgag tcatccatgc aaaacgacta ttgccacgcg
     5701 ccaatggacc aacataataa ggaattcgaa aagtcaagat tttttcaatc ttctcacgat
     5761 tgtcttttaa aaatggataa aagtcttctt gtcttctcaa aatagcatgc agctcaccca
     5821 agtgaatttg atggggaata gagccgttgt caaaggtccg ttgcttgcgc agcaaatctt
     5881 cacgatttag tttcaccaat aattcctcag taccatccat tttttctaaa attggtttga
     5941 taaatttata aaattcttct tggctagctc ccccatcaat ataacctgca tatccgtttt
     6001 ttgattgatc aaaaaagatt tctttatact tttctggaag ttgttgtcga actaaagctt
     6061 ttaaaagagt caagtcttga tgatgttcat cgtagcgttt aatcattgaa gctgataggg
     6121 gagccttagt tatttcagta tttactctta ggatatctga aagtaaaata gcatctgata
     6181 aattcttagc tgccaaaaac aaatcagcat attgatctcc aatttgcgcc aataaattat
     6241 ctaaatcatc atcgtaagta tcttttgaaa gctgtaattt agcatcttct gccaaatcaa
     6301 aatttgattt aaaattaggg gtcaaaccca atgacaaagc aatgagattc ccaaataagc
     6361 catttttctt ctcaccgggg agctgagcaa tgagattttc taatcgtctt gatttactca
     6421 atcgtgcaga aagaatcgct ttagcatcta ctccacttgc gttaataggg ttttcttcaa
     6481 ataattgatt gtaggtttgt accaactgga taaatagttt gtccacatca ctattatcag
     6541 gatttaaatc tccctcaatc aaaaaatgac cacgaaactt aatcatatgc gctaaggcca
     6601 aatagattaa gcgcaaatcc gctttatcag tagaatctac caattttttt cgcagatgat
     6661 agatagttgg atatttctca tgataagcaa cttcatctac tatatttcca aaaataggat
     6721 gacgttcatg cttcttgtct tcttccacca aaaaagactc ttcaagtcga tgaaagaaac
     6781 tatcatctac tttcgccatc tcatttgaaa aaatctcctg tagataacaa atacgattct
     6841 tccgacgtgt ataccttcta cgagctgtcc gtttgagacg agtcgcttcc gctgtctctc
     6901 cactgtcaaa taaaagagcc cctataagat tttttttgat actgtggc
//
