LOCUS       sequence-396954-       12973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of HA-tagged human SLC7A11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12973)
  AUTHORS   Jourdain AA, Begg BE, Mick E, Shah H, Calvo SE, Skinner OS, Sharma 
            R, Blue SM, Yeo GW, Burge CB, Mootha VK
  TITLE     Loss of LUC7L2 and U1 snRNP subunits shifts energy metabolism from 
            glycolysis to OXPHOS.
  JOURNAL   Mol Cell. 2021 May 6;81(9):1905-1919.e12. doi: 
            10.1016/j.molcel.2021.02.033. Epub 2021 Apr 13.
  PUBMED    33852893
REFERENCE   2  (bases 1 to 12973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.02.033"; journalName: "Mol Cell"; date: 
            "2021-05-6- 6"; volume: "81"; issue: "9"; pages: "1905-1919.e12"
COMMENT     Sequence Label: pWPI-SLC7A11-HA
FEATURES             Location/Qualifiers
     source          1..12973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             5038..5064
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5104..5671
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5122..5673
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5266..5283)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5493..5512
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5676..6470
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5730..5749)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6340..6359
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6644..6765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6804..7392
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6857..6877)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7275..7286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7536..7569)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7589..7769
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7794..7812)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7795..7812)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8102..8121)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8335..8354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8454..8476
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8514..8532)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8600..8704
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8705..8773,8774..9565)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8923..8942)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9736..10324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10225..10244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10478..10495
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10565..10585)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10570..10899
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10750..10885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12033..12098
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12228..12248
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12673..12807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttcata tgtacagagg atccatggtc
     3541 agaaagcctg ttgtgtccac catctccaaa ggaggttacc tgcagggaaa tgttaacggg
     3601 aggctgcctt ccctaggcaa caaggagcca cctgggcagg agaaagtgca gctgaagagg
     3661 aaagtcactt tactgagggg agtctccatt atcattggca ccatcattgg agcaggaatc
     3721 ttcatctctc ctaagggcgt gctccagaac acgggcagcg tgggcatgtc tctgaccatc
     3781 tggacggtgt gtggggtcct gtcactattt ggagctttgt cttatgctga attgggaaca
     3841 actataaaga aatctggagg tcattacaca tatattttgg aagtctttgg tccattacca
     3901 gcttttgtac gagtctgggt ggaactcctc ataatacgcc ctgcagctac tgctgtgata
     3961 tccctggcat ttggacgcta cattctggaa ccatttttta ttcaatgtga aatccctgaa
     4021 cttgcgatca agctcattac agctgtgggc ataactgtag tgatggtcct aaatagcatg
     4081 agtgtcagct ggagcgcccg aatccagatt ttcttaacct tttgcaagct cacagcaatt
     4141 ctgataatta tagtccctgg agttatgcag ctaattaaag gtcaaacgca gaactttaaa
     4201 gacgcctttt caggaagaga ttcaagtatt acgcggttgc cactggcttt ttattatgga
     4261 atgtatgcat atgctggctg gttttacctc aactttgtta ctgaagaagt agaaaaccct
     4321 gaaaaaacca ttccccttgc aatatgtata tccatggcaa ttgtcaccat tggctatgtg
     4381 ctgacaaatg tggcctactt tacgaccatt aatgctgagg agctgctgct ttcaaatgca
     4441 gtggcagtga ccttttctga gcggctactg ggaaatttct cattagcagt tccgatcttt
     4501 gttgccctct cctgctttgg ctccatgaac ggtggtgtgt ttgctgtctc caggttattc
     4561 tatgttgcgt ctcgagaggg tcaccttcca gaaatcctct ccatgattca tgtccgcaag
     4621 cacactcctc taccagctgt tattgttttg caccctttga caatgataat gctcttctct
     4681 ggagacctcg acagtctttt gaatttcctc agttttgcca ggtggctttt tattgggctg
     4741 gcagttgctg ggctgattta tcttcgatac aaatgcccag atatgcatcg tcctttcaag
     4801 gtgccactgt tcatcccagc tttgttttcc ttcacatgcc tcttcatggt tgccctttcc
     4861 ctctattcgg acccatttag tacagggatt ggcttcgtca tcactctgac tggagtccct
     4921 gcgtattatc tctttattat atgggacaag aaacccaggt ggtttagaat aatgtcagag
     4981 aaaataacca gaacattaca aataatactg gaagttgtac cagaagaaga taagttatat
     5041 ccatatgatg ttccagatta tgcttaatag tgaactagtc ccgggactac gggctgcagg
     5101 aattccgncc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     5161 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     5221 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     5281 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     5341 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     5401 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     5461 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     5521 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     5581 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga
     5641 cgtggttttc ctttgaaaaa cacgatgata ataccatgat tgaacaagat ggattgcacg
     5701 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5761 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5821 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     5881 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     5941 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6001 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6061 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     6121 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     6181 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     6241 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6301 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6361 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6421 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6481 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6541 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6601 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6661 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6721 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6781 gtcgacctcg agggaattcc gataatcaac ctctggatta caaaatttgt gaaagattga
     6841 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6901 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6961 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7021 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7081 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7141 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     7201 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7261 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7321 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7381 ccgcctcccc gcatcgggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     7441 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7501 caacgaagac aagatgggat caattcacca tgggaataac ttcgtatagc atacattata
     7561 cgaagttatg ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7621 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7681 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7741 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     7801 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     7861 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     7921 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     7981 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     8041 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     8101 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     8161 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     8221 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     8281 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     8341 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     8401 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     8461 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     8521 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     8581 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8641 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8701 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8761 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8821 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8881 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8941 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9001 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9061 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9121 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9181 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9241 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9301 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9361 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9421 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9481 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9541 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9601 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9661 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9721 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9781 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9841 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9901 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9961 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10021 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10081 cttggagcga acaacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10141 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10201 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10261 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10321 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10381 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    10441 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    10501 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    10561 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    10621 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    10681 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    10741 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    10801 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    10861 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
    10921 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
    10981 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
    11041 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
    11101 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
    11161 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
    11221 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
    11281 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
    11341 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
    11401 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
    11461 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
    11521 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
    11581 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
    11641 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
    11701 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
    11761 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
    11821 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
    11881 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
    11941 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
    12001 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
    12061 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
    12121 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
    12181 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
    12241 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
    12301 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
    12361 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
    12421 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
    12481 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
    12541 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
    12601 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    12661 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    12721 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    12781 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    12841 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    12901 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    12961 acaaacttag tag
//
