LOCUS       sequence-396959-        9799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1868
FEATURES             Location/Qualifiers
     source          1..9799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2737..3325
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2790..2810)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3208..3219)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3397..3630
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3708..3829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3745..3764)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3799..3818
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3869..4004
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4024..4043)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4025..4043)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4053..4070)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4053..4069)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4062..4084)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4211..4666
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4298..4317)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4508..4529
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4692..4796
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4797..4865,4866..5657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5015..5034)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5828..6416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6317..6336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6570..6587
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6704..6725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6740..6745,6746..6763,6764..6770)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6778..6794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6783..6805
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6802..6818
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6802..6818
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6837..6857
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6839..6857
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6885..7111
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7112..7292
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7339..7464
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7957..8190
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8375..8419
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8650..8860
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8672..8868
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8719..8854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8900..9502
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8900..8919)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9396..9416
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9545..9662
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 gagtcttcca atcctactgt tgctgtgcgt ggcagtttgc tcagcctatc cattggatgg
     1261 agctgcaagg ggtgaggaca ccagcatgaa ccttgttcag aaatatctag aaaactacta
     1321 cgacctcaaa aaagatgtga aacagtttgt taggagaaag gacagtggtc ctgttgttaa
     1381 aaaaatccga gaaatgcaga agttccttgg attggaggtg acggggaagc tggactccga
     1441 cactctggag gtgatgcgca agcccaggtg tggagttcct gatgttggtc acttcagaac
     1501 ctttcctggc atcccgaagt ggaggaaaac ccaccttaca tacaggattg tgaattatac
     1561 accagatttg ccaaaagatg ctgttgattc tgctgttgag aaagctctga aagtctggga
     1621 agaggtgact ccactcacat tctccaggct gtatgaagga gaggctgata taatgatctc
     1681 ttttgcagtt agagaacatg gagactttta cccttttgat ggacctggaa atgttttggc
     1741 ccatgcctat gcccctgggc cagggattaa tggagatgcc cactttgatg atgatgaaca
     1801 atggacaaag gatacaacag ggaccaattt atttctcgtt gctgctcatg aaattggcca
     1861 ctccctgggt ctctttcact cagccaacac tgaagctttg atgtacccac tctatcactc
     1921 actcacagac ctgactcggt tccgcctgtc tcaagatgat ataaatggca ttcagtccct
     1981 ctatggacct ccccctgact cccctgagac ccccctggta cccacggaac ctgtccctcc
     2041 agaacctggg acgccagcca actgtgatcc tgctttgtcc tttgatgctg tcagcactct
     2101 gaggggagaa atcctgatct ttaaagacag gcacttttgg cgcaaatccc tcaggaagct
     2161 tgaacctgaa ttgcatttga tctcttcatt ttggccatct cttccttcag gcgtggatgc
     2221 cgcatatgaa gttactagca aggacctcgt tttcattttt aaaggaaatc aattctgggc
     2281 tatcagagga aatgaggtac gagctggata cccaagaggc atccacaccc taggtttccc
     2341 tccaaccgtg aggaaaatcg atgcagccat ttctgataag gaaaagaaca aaacatattt
     2401 ctttgtagag gacaaatact ggagatttga tgagaagaga aattccatgg agccaggctt
     2461 tcccaagcaa atagctgaag actttccagg gattgactca aagattgatg ctgtttttga
     2521 agaatttggg ttcttttatt tctttactgg atcttcacag ttggagtttg acccaaatgc
     2581 aaagaaagtg acacacactt tgaagagtaa cagctggctt aattgttagt aatgaactag
     2641 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagcc
     2701 tcgtttagtt acgaccagaa cacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     2761 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2821 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2881 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2941 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3001 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3061 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3121 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3181 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3241 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3301 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     3361 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3421 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     3481 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3541 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3601 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     3661 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     3721 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3781 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     3841 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3901 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3961 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     4021 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     4081 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4141 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4201 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4261 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4321 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4381 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4441 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4501 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4561 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4621 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     4681 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4741 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4801 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4861 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4921 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4981 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5041 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5101 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5161 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5221 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5281 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5341 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5401 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5461 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5521 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5581 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5641 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5701 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5761 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5941 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6421 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6481 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6541 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6601 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6661 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6721 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6781 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     6841 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     6901 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     6961 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     7021 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     7081 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     7141 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7201 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7261 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7321 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7381 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7441 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7501 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7561 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7621 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7681 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7741 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7801 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     7861 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     7921 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     7981 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     8041 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     8101 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     8161 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     8221 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     8281 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     8341 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     8401 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     8461 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     8521 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     8581 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8641 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     8701 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8761 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8821 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     8881 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8941 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     9001 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     9061 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     9121 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     9181 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     9241 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     9301 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     9361 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     9421 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     9481 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     9541 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9601 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9661 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
     9721 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     9781 cttgaaagga gtgggaatt
//
