LOCUS       sequence-396962-       10183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2070
FEATURES             Location/Qualifiers
     source          1..10183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3121..3709
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3174..3194)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3592..3603)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3781..4014
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4092..4213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4129..4148)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4183..4202
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4253..4388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4408..4427)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4409..4427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4437..4454)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4437..4453)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4446..4468)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4595..5050
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4682..4701)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4892..4913
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5076..5180
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5181..5249,5250..6041)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5399..5418)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6212..6800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6701..6720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6954..6971
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7088..7109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7124..7129,7130..7147,7148..7154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7162..7178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7167..7189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7186..7202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7186..7202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7221..7241
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7223..7241
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7269..7495
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7496..7676
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7723..7848
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8341..8574
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8759..8803
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9034..9244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9056..9252
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9103..9238
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9284..9886
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9284..9303)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9780..9800
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9929..10046
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 ccgaagaccc cggcacagca tatatagcag tgacgaggat gatgaggact ttgagatgtg
     1261 tgaccatgac tatgatgggc tgcttcccaa gtctggaaag cgtcacttgg ggaaaacaag
     1321 gtggacccgg gaagaggatg aaaaactgaa gaagctggtg gaacagaatg gaacagatga
     1381 ctggaaagtt attgccaatt atctcccgaa tcgaacagat gtgcagtgcc agcaccgatg
     1441 gcagaaagta ctaaaccctg agctcatcaa gggtccttgg accaaagaag aagatcagag
     1501 agtgatagag cttgtacaga aatacggtcc gaaacgttgg tctgttattg ccaagcactt
     1561 aaaggggaga attggaaaac aatgtaggga gaggtggcat aaccacttga atccagaagt
     1621 taagaaaacc tcctggacag aagaggaaga cagaattatt taccaggcac acaagagact
     1681 ggggaacaga tgggcagaaa tcgcaaagct actgcctgga cgaactgata atgctatcaa
     1741 gaaccactgg aattctacaa tgcgtcggaa ggtcgaacag gaaggttatc tgcaggagtc
     1801 ttcaaaagcc agccagccag cagtggccac aagcttccag aagaacagtc atttgatggg
     1861 ttttgctcag gctccgccta cagctcaact ccctgccact ggccagccca ctgttaacaa
     1921 cgactattcc tattaccaca tttctgaagc acaaaatgtc tccagtcatg ttccataccc
     1981 tgtagcgtta catgtaaata tagtcaatgt ccctcagcca gctgccgcag ccattcagac
     2041 acagaaccac acatgcagct accccgggtg gcacagcacc accattgccg accacaccag
     2101 acctcatgga gacagtgcac ctgtttcctg tttgggagaa caccactcca ctccatctct
     2161 gccagcggat cctggctccc tacctgaaga aagcgcctcg ccagcaaggt gcatgatcgt
     2221 ccaccagggc accattctgg ataatgttaa gaacctctta gaatttgcag aaacactcca
     2281 atttatagat tctttcttaa acacttccag taaccatgaa aactcagact tggaaatgcc
     2341 ttctttaact tccacccccc tcattggtca caaattgact gttacaacac catttcatag
     2401 agaccagact gtgaaaactc aaaaggaaaa tactgttttt agaaccccag ctatcaaaag
     2461 gtcaatctta gaaagctctc caagaactcc tacaccattc aaacatgcac ttgcagctca
     2521 agaaattaaa tacggtcccc tgaagatgct acctcagaca ccctctcatc tagtagaaga
     2581 tctgcaggat gtgatcaaac aggaatctga tgaatctgga attgttgctg agtttcaaga
     2641 aaatggacca cccttactga agaaaatcaa acaagaggtg gaatctccaa ctgataaatc
     2701 aggaaacttc ttctgctcac accactggga aggggacagt ctgaataccc aactgttcac
     2761 gcagacctcg cctgtggcag atgcaccgaa tattcttaca agctccgttt taatggcacc
     2821 agcatcagaa gatgaagaca atgttctcaa agcatttaca gtacctaaaa acaggtccct
     2881 ggcgagcccc ttgcagcctt gtagcagtac ctgggaacct gcatcctgtg gaaagatgga
     2941 ggagcagatg acatcttcca gtcaagctcg taaatacgtg aatgcattct cagcccggac
     3001 gctggtcatg tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     3061 tatatcttgt ggaaaggacg agctcccact cctcgatggt cgcaaacgcg ttaagtcgac
     3121 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3181 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3241 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3301 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3361 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3421 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3481 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3541 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3601 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3661 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     3721 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     3781 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     3841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     4021 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     4081 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4141 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4201 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     4261 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4321 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4381 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     4441 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     4501 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     4561 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4621 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4681 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4741 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4801 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4861 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4921 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4981 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5041 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5101 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5161 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5221 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5281 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5341 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5401 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5461 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5521 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5581 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5641 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5701 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5761 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5821 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5881 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5941 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6001 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6061 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6121 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6601 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6841 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6901 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6961 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7021 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7081 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7141 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     7201 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     7261 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     7321 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     7381 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     7441 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     7501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     7681 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     7741 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     7801 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     7861 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     7921 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     7981 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     8041 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     8101 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     8161 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     8221 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     8281 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     8341 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     8401 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     8461 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     8521 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     8581 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     8641 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     8701 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     8761 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     8821 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     8881 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     8941 atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct caattagtca
     9001 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt
     9061 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9121 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9181 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9241 ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc
     9301 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     9361 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     9421 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     9481 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     9541 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     9601 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     9661 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     9721 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     9781 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     9841 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct
     9901 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     9961 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    10021 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
    10081 ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct
    10141 ttgcagctaa tggaccttct aggtcttgaa aggagtggga att
//
