LOCUS       sequence-396963-       10201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2110
FEATURES             Location/Qualifiers
     source          1..10201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3139..3727
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3192..3212)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3610..3621)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3799..4032
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4110..4231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4147..4166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4201..4220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4271..4406
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4426..4445)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4427..4445)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4455..4472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4455..4471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4464..4486)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4613..5068
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4700..4719)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4910..4931
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5094..5198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5199..5267,5268..6059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5417..5436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6230..6818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6719..6738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6972..6989
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7106..7127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7142..7147,7148..7165,7166..7172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7180..7196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7185..7207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7204..7220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7204..7220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7239..7259
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7241..7259
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7287..7513
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7514..7694
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7741..7866
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8359..8592
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8777..8821
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9052..9262
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9074..9270
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9121..9256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9302..9904
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9302..9321)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9798..9818
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9947..10064
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtg
     1201 gttggccaag gcctgttggt ccatccagag tgagatgccc tgtatccaag cccaatatgg
     1261 gacaccagca ccgagtccgg gaccccgtga ccacctggca agcgaccccc tgacccctga
     1321 gttcatcaag cccaccatgg acctggccag ccccgaggca gcccccgctg cccccactgc
     1381 cctgcccagc ttcagcacct tcatggacgg ctacacagga gagtttgaca ccttcctcta
     1441 ccagctgcca ggaacagtcc agccatgctc ctcagcctcc tcctcggcct cctccacatc
     1501 ctcgtcctca gccacctccc ctgcctctgc ctccttcaag ttcgaggact tccaggtgta
     1561 cggctgctac cccggccccc tgagcggccc agtggatgag gccctgtcct ccagtggctc
     1621 tgactactat ggcagcccct gctcggcccc gtcgccctcc acgcccagct tccagccgcc
     1681 ccagctctct ccctgggatg gctccttcgg ccacttctcg cccagccaga cttacgaagg
     1741 cctgcgggca tggacagagc agctgcccaa agcctctggg cccccacagc ctccagcctt
     1801 cttttccttc agtcctccca ccggccccag ccccagcctg gcccagagcc ccctgaagtt
     1861 gttcccctca caggccaccc accagctggg ggagggagag agctattcca tgcctacggc
     1921 cttcccaggt ttggcaccca cttctccaca ccttgagggc tcggggatac tggatacacc
     1981 cgtgacctca accaaggccc ggagcggggc cccaggtgga agtgaaggcc gctgtgctgt
     2041 gtgtggggac aacgcttcat gccagcatta tggtgtccgc acatgtgagg gctgcaaggg
     2101 cttcttcaag cgcacagtgc agaaaaacgc caagtacatc tgcctggcta acaaggactg
     2161 ccctgtggac aagaggcggc gaaaccgctg ccagttctgc cgcttccaga agtgcctggc
     2221 ggtgggcatg gtgaaggaag ttgtccgaac agacagcctg aaggggcggc ggggccggct
     2281 accttcaaaa cccaagcagc ccccagatgc ctcccctgcc aatctcctca cttccctggt
     2341 ccgtgcacac ctggactcag ggcccagcac tgccaaactg gactactcca agttccagga
     2401 gctggtgctg ccccactttg ggaaggaaga tgctggggat gtacagcagt tctacgacct
     2461 gctctccggt tctctggagg tcatccgcaa gtgggcggag aagatccctg gctttgctga
     2521 gctgtcaccg gctgaccagg acctgttgct ggagtcggcc ttcctggagc tcttcatcct
     2581 ccgcctggcg tacaggtcta agccaggcga gggcaagctc atcttctgct caggcctggt
     2641 gctacaccgg ctgcagtgtg cccgtggctt cggggactgg attgacagta tcctggcctt
     2701 ctcaaggtcc ctgcacagct tgcttgtcga tgtccctgcc ttcgcctgcc tctctgccct
     2761 tgtcctcatc accgaccggc atgggctgca ggagccgcgg cgggtggagg agctgcagaa
     2821 ccgcatcgcc agctgcctga aggagcacgt ggcagctgtg gcgggcgagc cccagccagc
     2881 cagctgcctg tcacgtctgt tgggcaaact gcccgagctg cggaccctgt gcacccaggg
     2941 cctgcagcgc atcttctacc tcaagctgga ggacttggtg ccccctccac ccatcattga
     3001 caagatcttc atggacacgc tgcccttcta gtaatgaact agtccgtaac ttgaaagtat
     3061 ttcgatttct tggctttata tatcttgtgg aaaggacgag acagctggga catatgcgaa
     3121 tggacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3181 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3241 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3301 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3361 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3421 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3481 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3541 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3601 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3661 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3721 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3781 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3841 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3901 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3961 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4021 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     4081 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4141 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4201 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4261 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4321 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4381 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4441 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4501 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4561 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4621 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4681 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4741 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4801 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4861 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4921 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4981 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5041 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5161 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5221 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5281 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5341 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5401 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5461 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5521 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5581 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5641 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5701 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5761 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5821 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5881 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5941 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6001 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6061 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6121 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6841 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6901 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6961 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7021 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7081 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7141 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7201 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7261 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7321 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7381 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7441 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7501 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7561 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7621 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7681 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7741 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7801 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7861 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7921 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7981 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8041 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8101 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8161 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     8221 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8281 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8341 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8401 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8461 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8521 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8581 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8641 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8701 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8761 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8821 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8881 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8941 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     9001 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     9061 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     9121 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9181 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9241 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     9301 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     9361 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     9421 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     9481 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     9541 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     9601 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     9661 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     9721 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     9781 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9841 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
     9901 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9961 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    10021 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    10081 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
    10141 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
    10201 t
//
