LOCUS       sequence-397007-       15803 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transfer plasmid for lentiviral vector production expressing Hs 
            ATP10B G671R/N865K mutants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Wouters R, Beletchi I, Van den Haute C, Baekelandt V, Martin S, 
            Eggermont J, Vangheluwe P
  TITLE     The lipid flippase ATP10B enables cellular lipid uptake under stress
            conditions.
  JOURNAL   Biochim Biophys Acta Mol Cell Res. 2024 Feb;1871(2):119652. doi: 
            10.1016/j.bbamcr.2023.119652. Epub 2023 Dec 11.
  PUBMED    38086447
REFERENCE   2  (bases 1 to 15803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta Mol Cell Res."; date: "2024-02"; volume: 
            "1871(2):119652. doi"; pages: " 10.1016/j.bbamcr.2023.119652. Epub 
            2023 Dec 11"
COMMENT     Sequence Label: pLenti HsATP10B_G671R/N865K
FEATURES             Location/Qualifiers
     source          1..15803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1191..1210)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1424..1443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1543..1565
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1603..1621)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1689..1793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1794..1862,1863..2654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2012..2031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2825..3413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3314..3333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3567..3584
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3654..3674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3659..3988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3839..3974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5122..5187
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5317..5337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5762..5896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5799..5818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5853..5872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6065..6698
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6745..6870
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7363..7596
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7781..7825
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8118..8235
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8294..8597
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8598..8801
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8752..8772
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8798..8822
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      8890..8899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9931..9942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    13354..13906
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(13498..13515)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     13725..13744
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             13906..14508
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     14405..14425
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    14528..15116
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(14581..14601)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(14999..15010)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             15286..15466
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 acacaagagg aaactgagag ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc
       61 gttagttacg actctatgat cttctgacac aaattccatt tactcctcac cctatgactc
      121 agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct tttatatagt
      181 aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt caagaagaag
      241 agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta acttggttag
      301 aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga ctatacaata
      361 taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt aaatattatt
      421 gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag aaatgtgtat
      481 cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt gttattaaca
      541 caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt taaaattaga
      601 aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta catacgttct
      661 tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt tgttccaaag
      721 actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt acttttagtg
      781 atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca tttcacattg
      841 cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat ctcagaaaca
      901 catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt cagccttcct
      961 catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa
     1021 ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac agtataacaa
     1081 ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga tcctctacgc
     1141 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     1201 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     1261 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     1321 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     1381 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     1441 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1501 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1561 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1621 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1741 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1801 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1861 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1921 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1981 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2041 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2101 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2161 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2221 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2281 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2341 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2401 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2461 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2521 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2581 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2641 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2701 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2761 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2821 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2881 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2941 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3001 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3061 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3121 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3181 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3241 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3301 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3361 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3421 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3481 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3541 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3601 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     4021 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     4081 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     4141 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     4201 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     4261 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     4321 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     4381 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     4441 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     4501 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     4561 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     4621 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     4681 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     4741 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     4801 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     4861 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     4921 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     4981 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     5041 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     5101 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     5161 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     5221 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     5281 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     5341 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     5401 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     5461 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     5521 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     5581 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     5641 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5701 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5761 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     5941 cccataccct attaccactg ccaattacct agtggtttca tttactctaa acctgtgatt
     6001 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     6061 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     6121 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     6181 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     6241 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     6301 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     6361 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     6421 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     6481 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     6541 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6601 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6661 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6721 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6781 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     6841 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6901 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6961 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     7021 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     7081 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     7141 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     7201 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     7261 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7321 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7381 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7441 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7501 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7561 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7621 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7681 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7741 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7801 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7861 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7921 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     7981 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     8041 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     8101 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     8161 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     8221 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gggcgataag
     8281 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     8341 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     8401 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     8461 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     8521 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     8581 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     8641 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     8701 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     8761 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     8821 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagagga
     8881 tctgctagcg ccaccatggc cctctcagtg gactcatcgt ggcatcggtg gcagtggaga
     8941 gtcagagatg gcttccccca ttgtccatcg gaaaccacac cgctgctctc tccagagaaa
     9001 gggagacaga gctacaactt gacacagcag cgggtcgtgt tccccaacaa cagcatattc
     9061 catcaagatt gggaagaggt ctccaggaga taccctggca acagaacctg cacaaccaaa
     9121 tacaccctct tcaccttcct gccccggaat ctctttgagc aatttcatag atgggctaac
     9181 ctctatttcc tgttcctggt gattttgaac tggatgccct ccatggaagt cttccacaga
     9241 gaaatcacca tgttaccatt ggccattgtc ctgttcgtca tcatgatcaa ggatggcatg
     9301 gaggacttca agagacaccg ctttgataaa gcaataaact gctccaacat tcgaatttat
     9361 gaaagaaaag agcagaccta tgtgcagaag tgctggaagg atgtgcgcgt gggagacttc
     9421 atccaaatga aatgcaatga gattgtccca gcagacatac tcctcctttt ttcctctgac
     9481 cccaatggga tatgccatct ggaaactgcc agcttggatg gagagacaaa cctcaagcaa
     9541 agatgtgtcg tgaagggctt ctcacagcag gaggtacagt tcgaaccaga gcttttccac
     9601 aataccatcg tgtgtgagaa acccaacaac cacctcaaca aatttaaggg ttatatggag
     9661 catcctgacc agaccaggac tggctttggc tgtgagagtc ttctgcttcg aggctgcacc
     9721 atcagaaaca ccgagatggc tgttggcatt gtcatctatg caggccatga gacgaaagcc
     9781 atgctgaaca acagtggccc ccggtacaaa cgcagcaaga ttgagcggcg catgaatata
     9841 gacatcttct tctgcattgg gatcctcatc ctcatgtgcc ttattggagc tgtaggtcac
     9901 agcatctgga atgggacctt tgaagaacac cctcccttcg atgtgccaga tgccaatggc
     9961 agcttccttc ccagtgccct tgggggcttc tacatgttcc tcacaatgat catcctgctc
    10021 caggtgctga tccccatctc tttgtatgtc tccattgagc tggtgaagct cgggcaagtg
    10081 ttcttcttga gcaatgacct tgacctgtat gatgaagaga ccgatttatc cattcaatgt
    10141 cgagccctca acatcgcaga ggacttgggc cagatccagt acatcttctc cgataagacg
    10201 gggaccctga cagagaacaa gatggtgttc cgacgttgca ccatcatggg cagcgagtat
    10261 tctcaccaag aaaatgctaa gcgactggag accccaaagg agctggactc agatggtgaa
    10321 gagtggaccc aataccaatg cctgtccttc tcggctagat gggcccagga tccagcaact
    10381 atgagaagcc aaaaaggtgc tcagcctctg aggaggagcc agagtgcccg ggtgcccatc
    10441 cagggccact accggcaaag gtctatgggg caccgtgaaa gctcacagcc tcctgtggcc
    10501 ttcagcagct ccatagaaaa agatgtaact ccagataaaa acctactgac caaggttcga
    10561 gatgctgccc tgtggttgga gaccttgtca gacagcagac ctgccaaggc ttccctctcc
    10621 accacctcct ccattgctga tttcttcctt gccttaacca tctgcaactc tgtcatggtg
    10681 tccacaacca ccgagcccag gcagagggtc accatcaaac cctcaagcaa ggctctgggg
    10741 acgtccctgg agaagattca gcagctcttc cagaagttga agctattgag cctcagccag
    10801 tcattctcat ccactgcacc ctctgacaca gacctcgggg agagcttagg ggccaacgtg
    10861 gccaccacag actcggatga gagagatgat gcatctgtgt gcagtagagg tgactccact
    10921 gatgacggtg gctacaggag cagcatgtgg gaccagggcg acatcctgga gtctgggtca
    10981 ggcacttcct tggaggaggc attggaggcc ccagccacag acctggccag gcctgagttc
    11041 tgttacgagg ctgagagccc tgatgaggcc gccctggtgc acgctgccca tgcctacagc
    11101 ttcacactag tgtcccggac acctgagcag gtgactgtgc gcctgcccca gggcacctgc
    11161 ctcaccttca gcctcctctg caccctgggc tttgactctg tcaggaagag aatgtctgtg
    11221 gttgtgaggc acccactgac tggcgagatt gttgtctaca ccaagggtgc tgactcggtc
    11281 atcatggacc tgctggaaga cccagcctgc gtacctgaca ttaatatgga aaagaagctg
    11341 agaaaaatcc gagcccggac ccaaaagcat ctagacttgt atgcaagaga tggcctgcgc
    11401 acactatgca ttgccaagaa ggttgtaagc gaagaggact tccggagatg ggccagtttc
    11461 cggcgtgagg ctgaggcatc cctcgacaaa cgagatgagc ttctcatgga aactgcacag
    11521 catctggaga atcaactcac cttacttgga gccactggga tcgaagaccg gctgcaggaa
    11581 ggagttccag atacgattgc cactctgcgg gaggctggga tccagctctg ggtcctgact
    11641 ggagataagc aggagacagc ggtcaacatt gcccattcct gcagactgtt aaatcagacc
    11701 gacactgttt ataccatcaa tacagagaat caggagacct gtgaatccat cctcaattgt
    11761 gcattggaag agctaaagca atttcgtgaa ctacagaagc cagaccgcaa gctctttgga
    11821 ttccgcttac cttccaagac accatccatc acctcagaag ctgtggttcc agaagctgga
    11881 ttggtcatcg atgggaagac attgaatgcc atcttccagg gaaagctaga gaagaagttt
    11941 ctggaattga cccagtattg tcggtccgtc ctgtgctgcc gctccacgcc actccagaag
    12001 agtatgatag tcaagctggt gcgagacaag ttgcgcgtca tgaccctttc cataggtgat
    12061 ggagcaaatg atgtaagcat gattcaagct gctgatattg gaattggaat atctggacag
    12121 gaaggcatgc aggctgtcat gtccagcgac tttgccatca cccgctttaa gcatctcaag
    12181 aagttgctgc tcgtgcatgg ccactggtgt tactcgcgcc tggccaggat ggtggtgtac
    12241 tacctctaca agaacgtgtg ctacgtcaac ctgctcttct ggtatcagtt cttctgtggt
    12301 ttctccagct ccaccatgat tgattactgg cagatgatat tcttcaatct cttctttacc
    12361 tccttgcctc ctcttgtctt tggagtcctt gacaaagaca tctctgcaga aacactcctg
    12421 gcattgcctg agctatacaa gagtggccag aactctgagt gctataacct gtcgactttc
    12481 tggatttcta tggtggatgc attctaccag agcctcatct gtttctttat cccttacctg
    12541 gcctataagg gctctgatat agatgtcttt acctttggga caccaatcaa caccatctcc
    12601 ctcaccacaa tccttttgca ccaggcaatg gaaatgaaga catggaccat tttccacgga
    12661 gtcgtgctcc tcggcagctt cctgatgtac tttctggtat ccctcctgta caatgccacc
    12721 tgcgtcatct gcaacagccc caccaatccc tattgggtga tggaaggcca gctctcaaac
    12781 cccactttct acctcgtctg ctttctcaca ccagttgttg ctcttctccc aagatacttt
    12841 ttcctgtctc tgcaaggaac ttgtgggaag tctctaatct caaaagctca gaaaattgac
    12901 aaactccccc cagacaaaag aaacctggaa atccagagtt ggagaagcag acagaggcct
    12961 gcccctgtcc ccgaagtggc tcgaccaact caccacccag tgtcatctat cacaggacag
    13021 gacttcagtg ccagcacccc aaagagctct aaccctccca agaggaagca tgtggaagag
    13081 tcagtactcc acgaacagag atgtggcacg gagtgcatga gggatgactc atgctcaggg
    13141 gactcctcag ctcaactctc atccggggag cacctgctgg gacctaacag gataatggcc
    13201 tactcaagag gacagactga tatgtgccgg tgctcaaaga ggagcagcca tcgccgatcc
    13261 cagagttcac tgaccatata gtacctcgag cactagtccc cgggaccgcg gcacgcgtgc
    13321 tctagcccaa ttccgccccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
    13381 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
    13441 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
    13501 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
    13561 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
    13621 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
    13681 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
    13741 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
    13801 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
    13861 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggg caccgagtac
    13921 aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc
    13981 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag
    14041 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    14101 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    14161 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    14221 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    14281 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    14341 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
    14401 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    14461 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagg cgcgttgaat
    14521 tcccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
    14581 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
    14641 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
    14701 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
    14761 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
    14821 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
    14881 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
    14941 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
    15001 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
    15061 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
    15121 ggaattctgt cgactggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
    15181 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaaa
    15241 tcgtcgagag atgctgcata taagcagctg ctttttgctt gtactgggtc tctctggtta
    15301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    15361 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    15421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
    15481 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
    15541 tgacattata atagatttag caggaattga actaggagtg gagcacacag gcaaagctgc
    15601 agaagtactt ggaagaagcc accagagata ctcacgattc tgcacatacc tggctaatcc
    15661 cagatcctaa ggattacatt aagtttacta acatttatat aatgatttat agtttaaagt
    15721 ataaacttat ctaatttact attctgacag atattaatta atcctcaaat atcataagag
    15781 atgattacta ttatccccat tta
//
