LOCUS       sequence-397039-        6728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An adeno-associated viral vector expressing murine Gas6 fused Myc 
            and DDK epitopes from a synapsin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Owlett LD, Karaahmet B, Le L, Belcher EK, Dionisio-Santos D, 
            Olschowka JA, Elliott MR, O'Banion MK
  TITLE     Gas6 induces inflammation and reduces plaque burden but worsens 
            behavior in a sex-dependent manner in the APP/PS1 model of 
            Alzheimer's disease.
  JOURNAL   J Neuroinflammation. 2022 Feb 7;19(1):38. doi: 
            10.1186/s12974-022-02397-y.
  PUBMED    35130912
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neuroinflammation."; date: "2022-02-7"; volume: "19(1):38. doi"; 
            pages: " 10.1186/s12974-022-02397-y"
COMMENT     Sequence Label: pOTTC1663 - pAAV SYN1 mGas6-Myc-DDK
FEATURES             Location/Qualifiers
     source          1..6728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             2700..2729
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2748..2771
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2791..2807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2791..2807)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    2855..3443
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2908..2928)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3326..3337)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3475..3951
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3674..3693)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3991..4131
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3991..4120
                     /label=AAV2 ITR
     rep_origin      4206..4661
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4293..4312)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4503..4524
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4678..4697)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4797..4819
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4857..4875)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4943..5047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5048..5116,5117..5908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5266..5285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6079..6667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6568..6587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccggatc cgaagttatc gctagcacca
      661 tgccgccacc gcccgggccc gccgccgccc tgggcactgc gcttctgctg ctcctgctgg
      721 cttccgagtc ttctcacact gtgctgttgc gggcgcgtga ggcggcgcag tttctgcggc
      781 ccaggcagcg ccgcgcctac caagtcttcg aggaggccaa gcagggccac ctggaacggg
      841 agtgcgtgga ggaggtgtgc agcaaagagg aggccagaga ggtgttcgag aacgaccccg
      901 agacggagta tttctatcca cgatatcaag agtgcatgag aaaatatggc aggcctgaag
      961 aaaaaaaccc agatttcgcc aaatgtgttc agaacttgcc tgaccagtgc accccaaacc
     1021 cttgtgataa gaagggtact catatctgcc aagacctcat gggcaacttc ttctgcgtgt
     1081 gcacagatgg ctggggaggc cggctctgtg acaaagatgt caatgagtgt gtccagaaga
     1141 atgggggctg cagccaggtc tgccacaaca aaccaggaag cttccaatgt gcctgccata
     1201 gtggcttctc gcttgcatca gacggccaga cctgccaaga tatcgatgaa tgcacagact
     1261 cagacacctg tggggacgcg cgatgcaaga acttgccagg ctcctactct tgcctctgcg
     1321 atgagggata tacatacagc tccaaggaga agacctgcca agatgtggac gagtgccagc
     1381 aggatcgctg tgagcagacc tgtgtcaact ccccaggcag ctatacctgc cactgtgatg
     1441 ggcgaggggg cctaaaacta tccccagaca tggatacttg tgaggacatc ttaccatgtg
     1501 tgcccttcag catggccaag agcgtgaagt ccttgtacct gggccgcatg ttcagcggga
     1561 cccccgtgat tagactacgc ttcaagaggc ttcagcctac caggctgctg gctgaatttg
     1621 acttccgcac ttttgaccct gaaggagtcc tcttcttcgc tggaggccgt tcagacagca
     1681 cctggattgt cctgggccta agagctgggc ggcttgagct gcagcttcgg tacaatggcg
     1741 ttgggcgcat caccagcagc gggccaacca tcaaccacgg catgtggcaa actatctccg
     1801 tggaagagct ggaacgtaac cttgtcatca aggtcaacaa agatgctgta atgaagatcg
     1861 cggtagctgg ggagctgttt cagctggaga ggggcctcta tcacctgaat ctcaccgtgg
     1921 gcggcattcc cttcaaggag agtgagctcg tccagccgat taaccctcgc ctggatgggt
     1981 gcatgaggag ttggaactgg ctgaacgggg aagacagcgc catccaggag acagtcaagg
     2041 caaacacaaa aatgcagtgc ttctctgtga cagaaagggg ctccttcttc ccggggaatg
     2101 gatttgctac ctacaggctc aactacaccc gaacatcgct ggatgtcggc acggaaacca
     2161 cctgggaagt taaagttgtg gctcggatcc gccctgccac ggacacgggg gtgctgctgg
     2221 cgctggtggg ggacgacgat gtcgtcccca tctctgtggc cctagtcgac taccactcta
     2281 caaagaagct caagaagcag ttggtggtcc tggcagttga ggatgttgcc ctggcactga
     2341 tggaaatcaa ggtgtgcgac agccaggaac acacggtcac tgtctccctg cgggagggtg
     2401 aggccaccct agaagtggat ggcacaaagg gccagagtga agtgagcact gcccagctgc
     2461 aggagcgact ggacacactt aagacacatc tgcaaggctc tgtgcacacc tatgttggag
     2521 gcctgccaga agtatcggtg atttctgcac ccgtcactgc gttctaccgc ggatgcatga
     2581 ctctggaggt aaacgggaaa atcctggacc tggatacggc ctcgtacaag cacagtgaca
     2641 tcacctccca ctcctgcccg cctgtggagc atgccacccc cacgcgtacg cggccgctcg
     2701 agcagaaact catctcagaa gaggatctgg cagcaaatga tatcctggat tacaaggatg
     2761 acgacgataa ggtttaaacg gccggccgcg gtcatagctg tttcctgaac agatggcgcg
     2821 ccataacttc gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     2881 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2941 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3001 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3061 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3121 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3181 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3241 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg
     3301 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3361 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3421 ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat ctacgggtgg
     3481 catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac tccagtgccc
     3541 accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt gtccttctat
     3601 aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     3661 agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact
     3721 gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
     3781 ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
     3841 accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc accttggcct
     3901 cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct tctgattttg
     3961 taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     4021 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4081 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     4141 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4201 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4261 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
     4321 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4381 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     4441 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4501 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
     4561 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
     4621 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     4681 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     4741 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4801 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     4861 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4921 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4981 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5041 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5101 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5161 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5221 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5281 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5341 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5401 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5461 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5521 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5581 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5641 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5701 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5761 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5821 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5881 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5941 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6001 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6061 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6121 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6181 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6241 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6301 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6361 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6421 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6481 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6541 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6601 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6661 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6721 tcacatgt
//
