LOCUS       sequence-397041-       11444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express mCherry-BRCA1 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Li X, Chung CI, Yang J, Chaudhuri S, Munster PN, Shu X
  TITLE     ATM-SPARK: A GFP phase separation-based activity reporter of ATM.
  JOURNAL   Sci Adv. 2023 Mar;9(9):eade3760. doi: 10.1126/sciadv.ade3760. Epub 
            2023 Mar 1.
  PUBMED    36857446
REFERENCE   2  (bases 1 to 11444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-03"; volume: "9(9):eade3760. doi"; pages: " 
            10.1126/sciadv.ade3760. Epub 2023 Mar 1"
COMMENT     Sequence Label: pcDNA-BRCA1-mCherry
FEATURES             Location/Qualifiers
     source          1..11444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             990..1697
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1135..1153)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1403..1422
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    1707..1731
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7330..7354)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7396..7413)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7402..7626
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7759..7778)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7910..7957
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7965..8987
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9117..9238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9154..9173)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9208..9227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9287..9303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9287..9303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9300..9322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9311..9327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9335..9341,9342..9359,9360..9365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9380..9401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9518..9535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9689..10277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9769..9788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10448..11239,11240..11308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11071..11090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11309..11413)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttggtacca tggtgagcaa gggcgaggag gataacatgg
     1021 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1081 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1141 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1201 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1261 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1321 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1381 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1441 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1501 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1561 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     1621 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     1681 tggacgagct gtacaagggc gcgcccacaa gtttgtacaa aaaagcaggc ttcaccatgg
     1741 atttatctgc tcttcgcgtt gaagaagtac aaaatgtcat taatgctatg cagaaaatct
     1801 tagagtgtcc catctgtctg gagttgatca aggaacctgt ctccacaaag tgtgaccaca
     1861 tattttgcaa attttgcatg ctgaaacttc tcaaccagaa gaaagggcct tcacagtgtc
     1921 ctttatgtaa gaatgatata accaaaagga gcctacaaga aagtacgaga tttagtcaac
     1981 ttgttgaaga gctattgaaa atcatttgtg cttttcagct tgacacaggt ttggagtatg
     2041 caaacagcta taattttgca aaaaaggaaa ataactctcc tgaacatcta aaagatgaag
     2101 tttctatcat ccaaagtatg ggctacagaa accgtgccaa aagacttcta cagagtgaac
     2161 ccgaaaatcc ttccttgcag gaaaccagtc tcagtgtcca actctctaac cttggaactg
     2221 tgagaactct gaggacaaag cagcggatac aacctcaaaa gacgtctgtc tacattgaat
     2281 tgggatctga ttcttctgaa gataccgtta ataaggcaac ttattgcagt gtgggagatc
     2341 aagaattgtt acaaatcacc cctcaaggaa ccagggatga aatcagtttg gattctgcaa
     2401 aaaaggctgc ttgtgaattt tctgagacgg atgtaacaaa tactgaacat catcaaccca
     2461 gtaataatga tttgaacacc actgagaagc gtgcagctga gaggcatcca gaaaagtatc
     2521 agggtagttc tgtttcaaac ttgcatgtgg agccatgtgg cacaaatact catgccagct
     2581 cattacagca tgagaacagc agtttattac tcactaaaga cagaatgaat gtagaaaagg
     2641 ctgaattctg taataaaagc aaacagcctg gcttagcaag gagccaacat aacagatggg
     2701 ctggaagtaa ggaaacatgt aatgataggc ggactcccag cacagaaaaa aaggtagatc
     2761 tgaatgctga tcccctgtgt gagagaaaag aatggaataa gcagaaactg ccatgctcag
     2821 agaatcctag agatactgaa gatgttcctt ggataacact aaatagcagc attcagaaag
     2881 ttaatgagtg gttttccaga agtgatgaac tgttaggttc tgatgactca catgatgggg
     2941 agtctgaatc aaatgccaaa gtagctgatg tattggacgt tctaaatgag gtagatgaat
     3001 attctggttc ttcagagaaa atagacttac tggccagtga tcctcatgag gctttaatat
     3061 gtaaaagtga aagagttcac tccaaatcag tagagagtaa tattgaagac aaaatatttg
     3121 ggaaaaccta tcggaagaag gcaagcctcc ccaacttaag ccatgtaact gaaaatctaa
     3181 ttataggagc atttgttact gagccacaga taatacaaga gcgtcccctc acaaataaat
     3241 taaagcgtaa aaggagacct acatcaggcc ttcatcctga ggattttatc aagaaagcag
     3301 atttggcagt tcaaaagact cctgaaatga taaatcaggg aactaaccaa acggagcaga
     3361 atggtcaagt gatgaatatt actaatagtg gtcatgagaa taaaacaaaa ggtgattcta
     3421 ttcagaatga gaaaaatcct aacccaatag aatcactcga aaaagaatct gctttcaaaa
     3481 cgaaagctga acctataagc agcagtataa gcaatatgga actcgaatta aatatccaca
     3541 attcaaaagc acctaaaaag aataggctga ggaggaagtc ttctaccagg catattcatg
     3601 cgcttgaact agtagtcagt agaaatctaa gcccacctaa ttgtactgaa ttgcaaattg
     3661 atagttgttc tagcagtgaa gagataaaga aaaaaaagta caaccaaatg ccagtcaggc
     3721 acagcagaaa cctacaactc atggaaggta aagaacctgc aactggagcc aagaagagta
     3781 acaagccaaa tgaacagaca agtaaaagac atgacagtga tactttccca gagctgaagt
     3841 taacaaatgc acctggttct tttactaagt gttcaaatac cagtgaactt aaagaatttg
     3901 tcaatcctag ccttccaaga gaagaaaaag aagagaaact agaaacagtt aaagtgtcta
     3961 ataatgctga agaccccaaa gatctcatgt taagtggaga aagggttttg caaactgaaa
     4021 gatctgtaga gagtagcagt atttcactgg tacctggtac tgattatggc actcaggaaa
     4081 gtatctcgtt actggaagtt agcactctag ggaaggcaaa aacagaacca aataaatgtg
     4141 tgagtcagtg tgcagcattt gaaaacccca agggactaat tcatggttgt tccaaagata
     4201 atagaaatga cacagaaggc tttaagtatc cattgggaca tgaagttaac cacagtcggg
     4261 aaacaagcat agaaatggaa gaaagtgaac ttgatgctca gtatttgcag aatacattca
     4321 aggtttcaaa gcgccagtca tttgctctgt tttcaaatcc aggaaatgca gaagaggaat
     4381 gtgcaacatt ctctgcccac tctgggtcct taaagaaaca aagtccaaaa gtcacttttg
     4441 aatgtgaaca aaaggaagaa aatcaaggaa agaatgagtc taatatcaag cctgtacaga
     4501 cagttaatat cactgcaggc tttcctgtgg ttggtcagaa agataagcca gttgataatg
     4561 ccaaatgtag tatcaaagga ggctctaggt tttgtctatc atctcagttc agaggcaacg
     4621 aaactggact cattactcca aataaacatg gacttttaca aaacccatat cgtataccac
     4681 cactttttcc catcaagtca tttgttaaaa ctaaatgtaa gaaaaatctg ctagaggaaa
     4741 actttgagga acattcaatg tcacctgaaa gagaaatggg aaatgagaac attccaagta
     4801 cagtgagcac aattagccgt aataacatta gagaaaatgt ttttaaagga gccagctcaa
     4861 gcaatattaa tgaagtaggt tccagtacta atgaagtggg ctccagtatt aatgaaatag
     4921 gttccagtga tgaaaacatt caagcagaac taggtagaaa cagagggcca aaattgaatg
     4981 ctatgcttag attaggggtt ttgcaacctg aggtctataa acaaagtctt cctggaagta
     5041 attgtaagca tcctgaaata aaaaagcaag aatatgaaga agtagttcag actgttaata
     5101 cagatttctc tccatatctg atttcagata acttagaaca gcctatggga agtagtcata
     5161 catctcaggt ttgttctgag acacctgatg acctgttaga tgatggtgaa ataaaggaag
     5221 atactagttt tgctgaaaat gacattaagg aaagttctgc tgtttttagc aaaagcgtcc
     5281 agagaggaga gcttagcagg agtcctagcc ctttcaccca tacacatttg gctcagggtt
     5341 accgaagagg ggccaagaaa ttagagtcct cagaagagaa cttatctagt gaggatgaag
     5401 agcttccctg cttccaacac ttgttatttg gtaaagtaaa caatatacct tctcagtcta
     5461 ctaggcatag caccgttgct accgagtgtc tgtctaagaa cacagaggag aatttattat
     5521 cattgaagaa tagcttaaat gactgcagta accaggtaat attggcaaag gcatctcagg
     5581 aacatcacct tagtgaggaa acaaaatgtt ctgctagctt gttttcttca cagtgcagtg
     5641 aattggaaga cttgactgca aatacaaaca cccaggatcc tttcttgatt ggttcttcca
     5701 aacaaatgag gcatcagtct gaaagccagg gagttggtct gagtgacaag gaattggttt
     5761 cagatgatga agaaagagga acgggcttgg aagaaaataa tcaagaagag caaagcatgg
     5821 attcaaactt aggtgaagca gcatctgggt gtgagagtga aacaagcgtc tctgaagact
     5881 gctcagggct atcctctcag agtgacattt taaccactca gcagagggat accatgcaac
     5941 ataacctgat aaagctccag caggaaatgg ctgaactaga agctgtgtta gaacagcatg
     6001 ggagccagcc ttctaacagc taccctagta taatcagtga ctcttctgcc cttgaggacc
     6061 tgcgaaatcc agaacaaagc acatcagaaa aagcagtatt aacttcacag aaaagtagtg
     6121 aataccctat aagccagaat ccagaaggcc tttctgctga caagtttgag gtgtctgcag
     6181 atagttctac cagtaaaaat aaagaaccag gagtggaaag gtcatcccct tctaaatgcc
     6241 catcattaga tgataggtgg tacatgcaca gttgctctgg gagtcttcag aatagaaact
     6301 acccatctca agaggagctc attaaggttg ttgatgtgga ggagcaacag ctggaagagt
     6361 ctgggccaca cgatttgacg gaaacatctt acttgccaag gcaagatcta gagggaaccc
     6421 cttacctgga atctggaatc agcctcttct ctgatgaccc tgaatctgat ccttctgaag
     6481 acagagcccc agagtcagct cgtgttggca acataccatc ttcaacctct gcattgaaag
     6541 ttccccaatt gaaagttgca gaatctgccc agggtccagc tgctgctcat actactgata
     6601 ctgctgggta taatgcaatg gaagaaagtg tgagcaggga gaagccagaa ttgacagctt
     6661 caacagaaag ggtcaacaaa agaatgtcca tggtggtgag tggcctgacc ccagaagaat
     6721 ttatgctcgt gtacaagttt gccagaaaac accacatcac tttaactaat ctaattactg
     6781 aagagactac tcatgttgtt atgaaaacag atgctgagtt tgtgtgtgaa cggacactga
     6841 aatattttct aggaattgcg ggaggaaaat gggtagttag ctatttctgg gtgacccagt
     6901 ctattaaaga aagaaaaatg ctgaatgagc atgattttga agtcagagga gatgtggtca
     6961 atggaagaaa ccaccaaggt ccaaagcgag caagagaatc ccaggacaga aagatcttca
     7021 gggggctaga aatctgttgc tatgggccct tcaccaacat gcccacagat caactggaat
     7081 ggatggtaca gctgtgtggt gcttctgtgg tgaaggagct ttcatcattc acccttggca
     7141 caggtgtcca cccaattgtg gttgtgcagc cagatgcctg gacagaggac aatggcttcc
     7201 atgcaattgg gcagatgtgt gaggcacctg tggtgacccg agagtgggtg ttggacagtg
     7261 tagcactcta ccagtgccag gagctggaca cctacctgat accccagatc ccccacagcc
     7321 actactgaga cccagctttc ttgtacaaag tggtgtgact cgagtctaga gggcccgttt
     7381 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     7441 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     7501 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     7561 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     7621 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     7681 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7741 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7801 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7861 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag aagttcctat
     7921 tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag cctgaactca
     7981 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
     8041 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     8101 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     8161 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggagttc agcgagagcc
     8221 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     8281 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     8341 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     8401 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     8461 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     8521 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     8581 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     8641 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     8701 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     8761 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     8821 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     8881 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     8941 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtactacgag
     9001 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     9061 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     9121 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9181 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9241 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     9301 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9361 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9421 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9481 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9541 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9601 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9661 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9721 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9781 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9841 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9901 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9961 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10021 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10081 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    10141 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10201 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10261 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10321 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10381 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10441 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10501 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10561 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10621 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10681 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10741 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10801 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    10861 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    10921 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    10981 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    11041 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    11101 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    11161 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11221 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11281 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11341 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11401 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
