LOCUS       sequence-397043-       12630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of 3xFLAG-tagged human LUC7L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Jourdain AA, Begg BE, Mick E, Shah H, Calvo SE, Skinner OS, Sharma 
            R, Blue SM, Yeo GW, Burge CB, Mootha VK
  TITLE     Loss of LUC7L2 and U1 snRNP subunits shifts energy metabolism from 
            glycolysis to OXPHOS.
  JOURNAL   Mol Cell. 2021 May 6;81(9):1905-1919.e12. doi: 
            10.1016/j.molcel.2021.02.033. Epub 2021 Apr 13.
  PUBMED    33852893
REFERENCE   2  (bases 1 to 12630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.02.033"; journalName: "Mol Cell"; date: 
            "2021-05-6- 6"; volume: "81"; issue: "9"; pages: "1905-1919.e12"
COMMENT     Sequence Label: pWPI-LUC7L-3xFLAG
FEATURES             Location/Qualifiers
     source          1..12630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4648..4713
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4761..5328
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4779..5330
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4923..4940)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5150..5169
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5333..6127
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5387..5406)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5997..6016
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6301..6422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6461..7049
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6514..6534)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6932..6943)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7193..7226)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7246..7426
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7451..7469)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7452..7469)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7759..7778)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7992..8011)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8111..8133
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8171..8189)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8257..8361
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8362..8430,8431..9222)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8580..8599)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9393..9981
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9882..9901
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10135..10152
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10222..10242)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10227..10556
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10407..10542
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11690..11755
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11885..11905
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12330..12464
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttcata tgtacagagg atccatgtcc
     3541 gcccaggcgc agatgcgggc cctgctggac cagctcatgg gcacggctcg ggacggagac
     3601 gaaaccagac agagggtcaa gtttacagat gaccgtgtct gcaagagtca ccttctggac
     3661 tgctgccccc atgacatcct ggctgggacg cgcatggatt taggagaatg taccaaaatc
     3721 cacgacttgg ccctccgagc agattatgag attgcaagta aagaaagaga cctgtttttt
     3781 gaattagatg caatggatca cttggagtcc tttattgctg aatgtgatcg gagaactgag
     3841 ctcgccaaga agcggctggc agaaacacag gaggaaatca gtgcggaagt ttctgcaaag
     3901 gcagaaaaag tacatgagtt aaatgaagaa ataggaaaac tccttgctaa agccgaacag
     3961 ctaggggctg aaggtaatgt ggatgaatcc cagaagattc ttatggaagt ggaaaaagtt
     4021 cgtgcgaaga aaaaagaagc tgaggaagaa tacagaaatt ccatgcctgc atccagtttt
     4081 cagcagcaaa agctgcgtgt ctgcgaggtc tgttcagcct accttggtct ccatgacaat
     4141 gaccgtcgcc tggcagacca cttcggtggc aagttacact tggggttcat tcagatccga
     4201 gagaagcttg atcagttgag gaaaactgtc gctgaaaagc aggagaagag aaatcaggat
     4261 cgcttgagga ggagagagga gagggaacgg gaggagcgtc tgagcaggag gtcgggatca
     4321 agaaccagag atcgcaggag gtcacgctcc cgggatcggc gtcggaggcg gtcaagatct
     4381 acctcccgag agcgacggaa attgtcccgg tcccggtccc gagatagaca tcggcgccac
     4441 cgcagccgtt cccggagcca cagccgggga catcgtcggg cttcccggga ccgaagtgcg
     4501 aaatacaagt tctccagaga gcgggcatcc agagaggagt cctgggagag cgggcggagc
     4561 gagcgagggc ccccggactg gaggcttgag agctccaacg ggaagatggc ttcacggagg
     4621 tcagaagaga aggaggccgg cgagatcgat tataaagatc atgatggcga ttataaagat
     4681 catgatattg attataaaga tgatgatgat aaataatagt gagcggccgc actagtcccg
     4741 ggactacggg ctgcaggaat tccgcccccc cccccctaac gttactggcc gaagccgctt
     4801 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     4861 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     4921 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     4981 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     5041 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     5101 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     5161 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     5221 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc
     5281 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata ccatgattga
     5341 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     5401 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5461 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     5521 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5581 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5641 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5701 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5761 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5821 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5881 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5941 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6001 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6061 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6121 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     6181 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6241 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     6301 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6361 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6421 tatcatgtct gtataccgtc gacctcgagg gaattccgat aatcaacctc tggattacaa
     6481 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6541 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6601 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6661 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6721 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6781 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6841 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6901 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6961 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7021 tcggatctcc ctttgggccg cctccccgca tcgggaattc gagctcggta cctttaagac
     7081 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7141 aagggctaat tcactcccaa cgaagacaag atgggatcaa ttcaccatgg gaataacttc
     7201 gtatagcata cattatacga agttatgctg ctttttgctt gtactgggtc tctctggtta
     7261 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7321 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7381 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
     7441 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
     7501 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
     7561 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
     7621 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
     7681 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     7741 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     7801 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     7861 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     7921 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
     7981 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     8041 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     8101 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     8161 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     8221 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     8281 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     8341 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     8401 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     8461 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     8521 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     8581 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     8641 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8701 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8761 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8821 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8881 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8941 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     9001 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     9061 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     9121 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     9181 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     9241 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     9301 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9361 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9421 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     9481 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     9541 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9601 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9661 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9721 tcgtgcacac agcccagctt ggagcgaaca acctacaccg aactgagata cctacagcgt
     9781 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9841 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9901 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9961 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    10021 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    10081 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    10141 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    10201 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
    10261 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
    10321 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    10381 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    10441 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    10501 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    10561 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
    10621 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
    10681 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
    10741 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
    10801 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
    10861 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
    10921 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
    10981 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
    11041 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
    11101 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
    11161 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
    11221 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
    11281 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
    11341 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
    11401 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
    11461 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
    11521 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
    11581 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
    11641 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
    11701 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
    11761 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
    11821 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
    11881 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
    11941 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
    12001 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
    12061 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
    12121 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
    12181 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
    12241 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    12301 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    12361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    12421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    12481 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    12541 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    12601 tttgttaaat atgtactaca aacttagtag
//
