LOCUS       sequence-397048-       12447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of 3xFLAG-tagged human UPP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12447)
  AUTHORS   Skinner OS, Blanco-Fernandez J, Goodman RP, Kawakami A, Shen H, 
            Kemeny LV, Joesch-Cohen L, Rees MG, Roth JA, Fisher DE, Mootha VK, 
            Jourdain AA
  TITLE     Salvage of ribose from uridine or RNA supports glycolysis in 
            nutrient-limited conditions.
  JOURNAL   Nat Metab. 2023 May;5(5):765-776. doi: 10.1038/s42255-023-00774-2. 
            Epub 2023 May 17.
  PUBMED    37198474
REFERENCE   2  (bases 1 to 12447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-023-00774-2"; journalName: "Nat Metab"; date: 
            "2023-05"; volume: "5"; issue: "5"; pages: "765-776"
COMMENT     Sequence Label: pWPI-UPP1-3xFLAG
FEATURES             Location/Qualifiers
     source          1..12447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4465..4530
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4578..5145
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4596..5147
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4740..4757)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4967..4986
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5150..5944
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5204..5223)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5814..5833
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6118..6239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6278..6866
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6331..6351)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6749..6760)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7010..7043)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7063..7243
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7268..7286)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7269..7286)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7576..7595)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7809..7828)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7928..7950
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7988..8006)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8074..8178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8179..8247,8248..9039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8397..8416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9210..9798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9699..9718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9952..9969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10039..10059)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10044..10373
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10224..10359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11507..11572
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11702..11722
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12147..12281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttcata tgtacagagg atccatggcc
     3541 gccaccggcg ccaacgccga gaaggccgag agccacaacg actgccccgt gaggctgctg
     3601 aaccccaaca tcgccaagat gaaggaggac atcctgtacc acttcaacct gaccaccagc
     3661 aggcacaact tccccgccct gttcggcgac gtgaagttcg tgtgcgtggg cggcagcccc
     3721 agcaggatga aggccttcat caggtgcgtg ggcgccgagc tgggcctgga ctgccccggc
     3781 agggactacc ccaacatctg cgccggcacc gacaggtacg ccatgtacaa ggtgggcccc
     3841 gtgctgagcg tgagccacgg catgggcatc cccagcatca gcatcatgct gcacgagctg
     3901 atcaagctgc tgtactacgc caggtgcagc aacgtgacca tcatcaggat cggcaccagc
     3961 ggcggcatcg gcctggagcc cggcaccgtg gtgatcaccg agcaggccgt ggacacctgc
     4021 ttcaaggccg agttcgagca gatcgtgctg ggcaagaggg tgatcaggaa gaccgacctg
     4081 aacaagaagc tggtgcagga gctgctgctg tgcagcgccg agctgagcga gttcaccacc
     4141 gtggtgggca acaccatgtg caccctggac ttctacgagg gccagggcag gctggacggc
     4201 gccctgtgca gctacaccga gaaggacaag caggcctacc tggaggccgc ctacgccgcc
     4261 ggcgtgagga acatcgagat ggagagcagc gtgttcgccg ccatgtgcag cgcctgcggc
     4321 ctgcaggccg ccgtggtgtg cgtgaccctg ctgaacaggc tggagggcga ccagatcagc
     4381 agccccagga acgtgctgag cgagtaccag cagaggcccc agaggctggt gagctacttc
     4441 atcaagaaga agctgagcaa ggccgattat aaagatcatg atggcgatta taaagatcat
     4501 gatattgatt ataaagatga tgatgataaa taatagtgag cggccgcact agtcccggga
     4561 ctacgggctg caggaattcc gccccccccc ccctaacgtt actggccgaa gccgcttgga
     4621 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     4681 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     4741 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     4801 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     4861 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     4921 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     4981 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     5041 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc
     5101 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatacca tgattgaaca
     5161 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5221 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5281 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     5341 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5401 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5461 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5521 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5581 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5641 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5701 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5761 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5821 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5881 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5941 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     6001 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     6061 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     6121 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6181 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6241 catgtctgta taccgtcgac ctcgagggaa ttccgataat caacctctgg attacaaaat
     6301 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6361 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6421 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6481 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6541 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6601 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6661 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     6721 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6781 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6841 gatctccctt tgggccgcct ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa
     6901 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6961 ggctaattca ctcccaacga agacaagatg ggatcaattc accatgggaa taacttcgta
     7021 tagcatacat tatacgaagt tatgctgctt tttgcttgta ctgggtctct ctggttagac
     7081 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7141 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7201 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc
     7261 cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat
     7321 tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga
     7381 agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct
     7441 tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg
     7501 tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     7561 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     7621 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     7681 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
     7741 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc
     7801 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     7861 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     7921 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     7981 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     8041 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8101 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8161 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     8221 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     8281 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     8341 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8401 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     8461 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8521 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     8581 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     8641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     8701 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     8761 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8881 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     9361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9541 tgcacacagc ccagcttgga gcgaacaacc tacaccgaac tgagatacct acagcgtgag
     9601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9841 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9901 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     9961 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10021 attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
    10081 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
    10141 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    10201 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    10261 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    10321 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
    10381 gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
    10441 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
    10501 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
    10561 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
    10621 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
    10681 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
    10741 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
    10801 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
    10861 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
    10921 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
    10981 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
    11041 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
    11101 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
    11161 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
    11221 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
    11281 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
    11341 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
    11401 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
    11461 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
    11521 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
    11581 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
    11641 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
    11701 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
    11761 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
    11821 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
    11881 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
    11941 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
    12001 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
    12061 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    12121 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    12181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    12241 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    12301 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    12361 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    12421 gttaaatatg tactacaaac ttagtag
//
