LOCUS       sequence-397049-       12468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-expression of 3xFLAG-tagged human UPP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Skinner OS, Blanco-Fernandez J, Goodman RP, Kawakami A, Shen H, 
            Kemeny LV, Joesch-Cohen L, Rees MG, Roth JA, Fisher DE, Mootha VK, 
            Jourdain AA
  TITLE     Salvage of ribose from uridine or RNA supports glycolysis in 
            nutrient-limited conditions.
  JOURNAL   Nat Metab. 2023 May;5(5):765-776. doi: 10.1038/s42255-023-00774-2. 
            Epub 2023 May 17.
  PUBMED    37198474
REFERENCE   2  (bases 1 to 12468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-023-00774-2"; journalName: "Nat Metab"; date: 
            "2023-05"; volume: "5"; issue: "5"; pages: "765-776"
COMMENT     Sequence Label: pWPI-UPP2-3xFLAG
FEATURES             Location/Qualifiers
     source          1..12468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4486..4551
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4599..5166
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4617..5168
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4761..4778)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4988..5007
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5171..5965
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5225..5244)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5835..5854
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6139..6260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6299..6887
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6352..6372)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6770..6781)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7031..7064)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7084..7264
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7289..7307)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7290..7307)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7597..7616)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7830..7849)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7949..7971
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8009..8027)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8095..8199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8200..8268,8269..9060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8418..8437)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9231..9819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9720..9739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9973..9990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10060..10080)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10065..10394
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10245..10380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11528..11593
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11723..11743
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12168..12302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttcata tgtacagagg atccatggcc
     3541 agcgtgatcc ccgccagcaa caggagcatg aggagcgaca ggaacaccta cgtgggcaag
     3601 aggttcgtgc acgtgaagaa cccctacctg gacctgatgg acgaggacat cctgtaccac
     3661 ctggacctgg gcaccaagac ccacaacctg cccgccatgt tcggcgacgt gaagttcgtg
     3721 tgcgtgggcg gcagccccaa caggatgaag gccttcgccc tgttcatgca caaggagctg
     3781 ggcttcgagg aggccgagga ggacatcaag gacatctgcg ccggcaccga caggtactgc
     3841 atgtacaaga ccggccccgt gctggccatc agccacggca tgggcatccc cagcatcagc
     3901 atcatgctgc acgagctgat caagctgctg caccacgcca ggtgctgcga cgtgaccatc
     3961 atcaggatcg gcaccagcgg cggcatcggc atcgcccccg gcaccgtggt gatcaccgac
     4021 atcgccgtgg acagcttctt caagcccagg ttcgagcagg tgatcctgga caacatcgtg
     4081 accaggagca ccgagctgga caaggagctg agcgaggagc tgttcaactg cagcaaggag
     4141 atccccaact tccccaccct ggtgggccac accatgtgca cctacgactt ctacgagggc
     4201 cagggcaggc tggacggcgc cctgtgcagc ttcagcaggg agaagaagct ggactacctg
     4261 aagagggcct tcaaggccgg cgtgaggaac atcgagatgg agagcaccgt gttcgccgcc
     4321 atgtgcggcc tgtgcggcct gaaggccgcc gtggtgtgcg tgaccctgct ggacaggctg
     4381 gactgcgacc agatcaacct gccccacgac gtgctggtgg agtaccagca gaggccccag
     4441 ctgctgatca gcaacttcat caggaggagg ctgggcctgt gcgacgatta taaagatcat
     4501 gatggcgatt ataaagatca tgatattgat tataaagatg atgatgataa ataatagtga
     4561 gcggccgcac tagtcccggg actacgggct gcaggaattc cgnccccccc cccctaacgt
     4621 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     4681 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4741 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4801 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4861 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4921 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4981 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     5041 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     5101 gttaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     5161 tgataatacc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5221 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5281 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5341 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5401 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5461 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5521 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5581 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5641 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5701 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5761 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5821 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5881 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5941 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6001 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6061 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6121 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6181 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6241 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctcgaggga attccgataa
     6301 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6361 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6421 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6481 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6541 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6601 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6661 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     6721 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6781 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6841 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gggaattcga
     6901 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6961 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat gggatcaatt
     7021 caccatggga ataacttcgt atagcataca ttatacgaag ttatgctgct ttttgcttgt
     7081 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7141 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7201 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7261 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
     7321 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
     7381 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
     7441 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
     7501 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
     7561 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     7621 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     7681 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     7741 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     7801 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7861 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     7921 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     7981 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     8041 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     8101 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8161 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8221 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8281 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8341 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8401 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8461 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8521 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8581 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8641 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8701 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     8761 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8821 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8881 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8941 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9001 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9061 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9121 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9181 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9241 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9301 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9361 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     9421 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9481 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9541 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacaac ctacaccgaa
     9601 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9661 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9721 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9781 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9841 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9901 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9961 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    10021 cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt
    10081 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    10141 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    10201 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
    10261 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    10321 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    10381 taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca
    10441 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
    10501 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
    10561 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
    10621 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
    10681 aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac
    10741 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
    10801 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
    10861 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg
    10921 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
    10981 cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
    11041 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc
    11101 gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
    11161 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
    11221 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac
    11281 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc
    11341 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac
    11401 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt
    11461 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
    11521 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
    11581 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
    11641 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
    11701 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
    11761 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
    11821 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
    11881 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
    11941 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
    12001 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
    12061 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
    12121 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    12181 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    12241 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    12301 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    12361 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    12421 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtag
//
