LOCUS       sequence-397075-       14079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a transgene encoding RUBY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14079)
  TITLE     Curtin lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pARSCL504
FEATURES             Location/Qualifiers
     source          1..14079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..59
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             61..4011
                     /label=RUBY
     CDS             1561..1617
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2452..2508
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    4052..4226
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(4244..4261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4244..4260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4253..4275)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4463..4487
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5788..6417
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6846..7919
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7985..8179
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8415..8437)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8523..8663
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8578..8597
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8678..8695)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8849..9437)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8929..8948)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9524..10318)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10743..10767
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10845..11019
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11026..11577)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(11622..12298)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    12489..12510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12525..12530,12531..12548,12549..12555)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12563..12579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12568..12590
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12587..12603
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12587..12603
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        13719..14064
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 acaacaatta ccaacaacaa caaacaacaa acaacattac aattactatt tacaattaca
       61 atggatcatg cgaccctcgc catgatcctc gcgatctggt tcatcagctt ccacttcatc
      121 aagctgctgt tctcccagca gaccaccaag ctgcttccgc caggaccaaa gccgcttccg
      181 atcatcggca acatccttga ggtgggcaag aagccgcatc ggtccttcgc caacctcgcc
      241 aagattcacg gcccactcat ttccctcaga ctcggctctg tgaccaccat cgttgtgtcc
      301 tctgccgacg tggccaaaga gatgttcctc aagaaggatc acccgctctc caaccgcacg
      361 atcccgaata gtgttacagc cggcgaccac cacaagctca ccatgtcttg gctcccggtg
      421 tctccgaagt ggcgcaactt ccgcaagatt accgccgtgc atctgctctc cccacagaga
      481 ctcgatgcct gccagacatt caggcacgcc aaggtgcagc agctctacga gtacgttcaa
      541 gagtgcgccc agaaaggcca ggccgtggat attggcaagg ccgcctttac gaccagcctc
      601 aacctcctca gcaagctgtt cttcagcgtc gagctggcgc accacaagtc ccataccagc
      661 caagagttca aagagctgat ctggaacatc atggaagata taggcaagcc gaactacgcc
      721 gactacttcc cgattctcgg ctgcgttgac ccatctggca ttagaagaag gctcgcctgc
      781 tccttcgaca agctgatcgc cgtgttccag ggcatcatct gcgagagact cgccccagat
      841 tcctccacca caactaccac caccaccgac gacgtgctcg atgtgctcct ccagctgttc
      901 aagcagaacg agctgacgat gggcgagatc aaccacctcc tcgtggacat cttcgacgcc
      961 ggcaccgata ccacatcctc cacattcgag tgggtgatga ccgagctgat ccgcaatcca
     1021 gagatgatgg aaaaggccca agaggaaatc aagcaggtcc tcggcaagga caagcagatc
     1081 caagagtccg acatcatcaa cctgccgtac ctccaggcga tcatcaaaga gacactccgc
     1141 ctccatccgc cgaccgtgtt cttgctccca agaaaggccg acaccgatgt cgagctgtac
     1201 ggctacatcg tgccgaagga tgcccagatc ctcgtgaacc tctgggccat tggcagggac
     1261 ccaaacgcct ggcagaacgc cgatattttc agcccagagc gcttcatcgg ctgcgagatc
     1321 gatgttaagg gccgcgattt cggcctcctt ccatttggcg ctggccgcag aatttgccca
     1381 ggcatgaatc tcgccatcag gatgctcacc ctcatgctcg ccacactcct ccagttcttc
     1441 aactggaagc tcgaaggcga catctccccg aaggacctcg acatggacga gaagttcggc
     1501 attgcgctcc aaaagaccaa gccgctcaag ctcatcccga ttccgcgcta cggtagcgga
     1561 gctaccaatt ttagcctcct taagcaggct ggtgatgtag aagagaaccc cgggcctatg
     1621 aagatgatga acggcgagga cgccaacgac cagatgatca aagagtcctt cttcatcacc
     1681 cacggcaacc cgatcctcac cgtcgaggat acacatccgc tcaggccgtt cttcgagaca
     1741 tggcgcgaga agattttctc caagaagccg aaggccatcc tcatcatctc cggccactgg
     1801 gagacagtga agccaaccgt gaacgccgtg cacatcaacg acaccatcca cgacttcgac
     1861 gactacccag ccgccatgta ccagttcaag tacccagctc caggcgagcc agagcttgcg
     1921 agaaaggtgg aagagatcct caagaagtcc gggttcgaga cagccgagac agaccaaaag
     1981 aggggccttg atcacggcgc ctgggttcca ctcatgctca tgtatccaga ggcggacatc
     2041 ccggtgtgcc agctctcagt tcagccacat ctcgacggca cctaccacta caatctcggc
     2101 agagccctcg cgccgctcaa gaatgatggc gtgctcatta ttggctccgg cagcgccaca
     2161 catccactcg atgagacacc gcactacttc gatggtgttg ccccttgggc cgctgccttc
     2221 gattcttggc ttaggaaggc cctcatcaac ggccgcttcg aggaagtgaa catctacgag
     2281 agcaaggccc cgaactggaa gctcgcccat ccatttccag agcacttcta cccgctccac
     2341 gttgtgctcg gcgctgctgg tgaaaagtgg aaggccgagc tgatccactc ctcctgggat
     2401 catggcacac tttgccacgg ctcctacaag ttcacctccg ccggatccgg agcaaccaac
     2461 tttagcctgc tcaagcaagc aggagatgtt gaggaaaatc ctggccccat gaccgccatc
     2521 aagatgaaca ccaacggcga gggcgagaca cagcacatcc tcatgatccc gttcatggcg
     2581 cagggccacc tcaggccatt tctcgaactc gccatgttcc tctacaagcg ctcccacgtg
     2641 atcatcaccc tgctcacaac tccgctcaat gccggcttcc tcaggcacct ccttcaccac
     2701 cattcctact cctccagcgg catcaggatc gtcgagctgc cattcaactc caccaaccac
     2761 ggactcccac cgggcatcga gaacaccgat aagctcacac tcccgctcgt ggtgtccctc
     2821 ttccattcca ccatcagcct cgatccgcac ctccgcgatt acatctccag gcatttcagc
     2881 ccagccaggc caccactctg cgtgatccat gatgtgttcc tcggctgggt tgaccaggtg
     2941 gccaaggatg tgggctctac aggcgtggtg ttcacaacag gcggcgctta tggcacatcc
     3001 gcctacgtgt ccatctggaa cgatctcccg caccagaact actccgacga ccaagagttc
     3061 ccgctgccag gcttcccaga gaaccataag ttccgcaggt cccagctcca tcggttcctc
     3121 agatatgccg acggctccga cgattggtcc aagtatttcc agccgcagct ccgccagtcc
     3181 atgaagtctt ttggctggct ctgcaactcc gtggaagaga tcgagacact cggcttctcc
     3241 atcctccgca actacaccaa gctgccgatc tggggcatcg gcccacttat tgcttcccca
     3301 gtgcagcact cctcctccga caacaattca acaggcgccg agttcgtgca gtggctcagc
     3361 ctcaaagagc cggactccgt cctctacatc tccttcggct cccagaacac gatcagcccg
     3421 acgcagatga tggaactcgc tgctggcctt gagtcctccg agaagccatt cctctgggtg
     3481 atcagagccc cgttcggctt cgacatcaac gaagagatgc gcccagagtg gctgccagag
     3541 ggctttgagg aacgcatgaa ggtgaagaaa cagggcaagc tcgtgtacaa gctcggcccg
     3601 cagcttgaga tcctcaacca tgaatccatc ggcggctttc tcacccactg cggatggaac
     3661 agcatccttg agtctcttcg cgagggcgtt ccgatgcttg gatggccact tgctgccgag
     3721 caggcctaca acctcaagta cctcgaagat gagatgggcg tcgcggttga gcttgctaga
     3781 ggcctcgaag gcgagatctc caaagagaag gtcaagcgca tcgtcgagat gatccttgag
     3841 cgcaacgagg gctccaaagg ctgggagatg aagaatcgcg ccgtggaaat gggcaaaaag
     3901 ctcaaggacg ccgtgaacga ggaaaaagag ctgaagggct cctccgtgaa ggcgatcgac
     3961 gatttcctcg acgccgtcat gcaggccaaa cttgagccaa gcctccagtg atagtgagct
     4021 tctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc
     4081 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
     4141 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     4201 caaaatccag tactaaaatc cagatcgctg agctcagtgt ggcactggcc gtcgttttac
     4261 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4321 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     4381 gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc
     4441 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     4501 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     4561 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     4621 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     4681 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     4741 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     4801 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     4861 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     4921 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     4981 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     5041 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     5101 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     5161 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     5221 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     5281 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     5341 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     5401 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     5461 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     5521 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     5581 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     5641 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     5701 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     5761 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     5821 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     5881 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     5941 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     6001 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     6061 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     6121 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     6181 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     6241 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     6301 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     6361 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     6421 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     6481 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     6541 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     6601 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     6661 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     6721 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     6781 gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     6841 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
     6901 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
     6961 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
     7021 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
     7081 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
     7141 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
     7201 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
     7261 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
     7321 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
     7381 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
     7441 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
     7501 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
     7561 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
     7621 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
     7681 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     7741 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     7801 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     7861 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     7921 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct
     7981 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     8041 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     8101 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     8161 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     8221 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     8281 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     8341 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
     8401 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     8461 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     8521 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     8581 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     8641 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8701 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8761 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8821 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8881 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8941 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9001 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9061 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     9121 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     9181 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     9241 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     9301 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9361 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9421 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9481 acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     9541 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     9601 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     9661 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     9721 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     9781 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     9841 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     9901 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     9961 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
    10021 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
    10081 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
    10141 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
    10201 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
    10261 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
    10321 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
    10381 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
    10441 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
    10501 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
    10561 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
    10621 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
    10681 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
    10741 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
    10801 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
    10861 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
    10921 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
    10981 cccttatctg ggaactactc acacattatt atggagaaac tcgagtcaaa tctcggtgac
    11041 gggcaggacc ggacggggcg gtaccggcag gctgaagtcc agctgccaga aacccacgtc
    11101 atgccagttc ccgtgcttga agccggccgc ccgcagcatg ccgcgggggg catatccgag
    11161 cgcctcgtgc atgcgcacgc tcgggtcgtt gggcagcccg atgacagcga ccacgctctt
    11221 gaagccctgt gcctccaggg acttcagcag atgggtgtag agcgtggagc ccagtcccgt
    11281 ccgctggtgg cgggggctga cgtacacggt cgactcggcc gtccagtcgt aggcgttgcg
    11341 tgccttccag gggcccgcgt aggcgatgcc ggcgacctcg ccgtccacct cggcgacgag
    11401 ccagggatag cgctcccgca gacggacgag gtcgtccgtc cactcctgcg gttcctgcgg
    11461 ctcggtacgg aagttgaccg tgcttgtctc gatgtagtgg ttgacgatgg tgcagaccgc
    11521 cggcatgtcc gcctcggtgg cacggcggat gtcggccggg cgtcgttctg ggctcatggt
    11581 agactcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt cctctccaaa
    11641 tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca
    11701 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
    11761 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
    11821 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
    11881 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
    11941 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
    12001 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttcac atcaatccac
    12061 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
    12121 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
    12181 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
    12241 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
    12301 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
    12361 ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcctctccc cgcgcgttgg
    12421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    12481 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    12541 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    12601 gacatgatta cgaattcgag ctcggtaccc ggggatcggc gcgcgcggcc gcacagagat
    12661 ttaaatagct ccggtgacgg acggcgcgac tagttttacg tacgttaatt aacccggggc
    12721 gcgccggagg aattccaatc ccacaaaaat ctgagcttaa cagcacagtt gctcctctca
    12781 gagcagaatc gggtattcaa caccctcata tcaactacta cgttgtgtat aacggtccac
    12841 atgccggtat atacgatgac tggggttgta caaaggcggc aacaaacggc gttcccggag
    12901 ttgcacacaa gaaatttgcc actattacag aggcaagagc agcagctgac gcgtacacaa
    12961 caagtcagca aacagacagg ttgaacttca tccccaaagg agaagctcaa ctcaagccca
    13021 agagctttgc taaggcccta acaagcccac caaagcaaaa agcccactgg ctcacgctag
    13081 gaaccaaaag gcccagcagt gatccagccc caaaagagat ctcctttgcc ccggagatta
    13141 caatggacga tttcctctat ctttacgatc taggaaggaa gttcgaaggt gaaggtgacg
    13201 acactatgtt caccactgat aatgagaagg ttagcctctt caatttcaga aagaatgctg
    13261 acccacagat ggttagagag gcctacgcag caagtctcat caagacgatc tacccgagta
    13321 acaatctcca ggagatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca
    13381 ggactaattg catcaagaac acagagaaag acatatttct caagatcaga agtactattc
    13441 cagtatggac gattcaaggc ttgcttcata aaccaaggca agtaatagag attggagtct
    13501 ctaaaaaggt agttcctact gaatctaagg ccatgcatgg agtctaagat tcaaatcgag
    13561 gatctaacag aactcgccgt caagactggc gaacagttca tacagagtct tttacgactc
    13621 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acactctggt ctactccaaa
    13681 aatgtcaaag atacagtctc agaagatcaa agggctattg agacttttca acaaaggata
    13741 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
    13801 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
    13861 caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
    13921 gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga catctccact
    13981 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
    14041 agttcatttc atttggagag gacacgctac tgtattttt
//
