LOCUS       sequence-397084-       15976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of CyCAST proteins with crRNA targeting 
            CyPSP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15976)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 15976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pMSIP116: pHelper(CyCAST)_PSP1
FEATURES             Location/Qualifiers
     source          1..15976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        290..308
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    309..333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      5957..6004
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6150..6168
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6169..6193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        12459..12477
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    12478..12502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             12611..12655
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      12707..12754
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(12875..13666)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(13667..13758)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13806..14544)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(14754..15836)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     15798..15817
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(15837..15914)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(15960..15976,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggtttcca agcctgaatc ccggtctacg ggactgaaac gagaagtcat
      121 ttaataaggc cactgttaaa cgtaggtttc caagcctgaa tcccggtcta cgggactgaa
      181 acgatccgaa ttcgagctcg gcgcgcctgc aggtcgacaa gcttgcggcc gcataatgct
      241 taagtcgaac agaaagtaat cgtattgtac acggccgcat aatcgaaatt aatacgactc
      301 actatagggg aattgtgagc ggataacaat tccccatctt agtatattag ttaagtataa
      361 gaaggagata tacatatggc taccctactc caaaccctcc tgttacgaac gttgtcaccc
      421 gaggcggatc cagttctcta tgtctacatt gagacggtac tacctgcaat ggaacgggaa
      481 tttggcttga ttccagccct tggtggctcg gaggaagcac actttcatct gctgaggcga
      541 cggggaaaca aatatgcggc ggaaaattcc aagcggtacg ccagtcgctc tgatcaaagc
      601 ctgctggttc atgtgctcaa tgccttgctg accgcctgga atttgttgcc attcctaagc
      661 tccacatttg aactatcaga tgatgagaaa cggctgctct gcctggggct gaccctacac
      721 gattacaaca agtggtgcca gggtgaggag gaagattcgc cgattgcatg cgaagtagca
      781 gagattcttg agctttgtca aacattggga gagcggttga actttgacgg attctgggca
      841 gagtggcggg agtatttgac agagattgcc taccttgccc aaaacaccca gggaaaagtt
      901 ggaacaaatc tacccaaagc gaattggggc agcttcaaaa ttctagatac ccgtcgtctg
      961 gattcgcccc tacgatggct actcgctttt ggcgatgtcg cagtccatct cagcgatcca
     1021 tctgacatcg caatgaaaac ggggggagat cgcctgcggg aacatctgcg ctttctaggt
     1081 attcgtcgca agctggtcta ccatcgcctg cgcaactgta cagggctatt aaccaacgga
     1141 attcataatg cggtaatgca ttttgctaga gaactggatt ggcaacccat cctcttcttt
     1201 gctcagggtg ttgtttatct cgctcctcag ggtgctggcg ttccggatcg cttagaactt
     1261 caggaatttc tctgggagca ggtcagcaac cgattagcca ccagcatgtt gcggggagag
     1321 gttggtttca aacgggatgg caagggactc aaggtggctc cccaaaccct ggaactgttc
     1381 tcccctgctc agttgattcg gaacttgccc ggtgttgtcc aatcttatgt cggaaacgct
     1441 aaatctcctg cgactccaaa acgacttgag aagctggaac tcagttcaac agagcgagag
     1501 tttttggcta aaggtgcaga tttaagagca gatcgcattg ccgagtttat ctttctggcg
     1561 caaaaagagt ttttcggaga taaaccagaa tttatcactt ggatgcttga aactctgggg
     1621 gttcaaactg ctatcacttc ggagcagacg cagcttcaat ccggtggtgt gaactgggga
     1681 tggtatcacg cagcagccta ttacgtcgcg agcaatgcca cgctggattt ggaagagatt
     1741 tcggagaaac ttcaggaatt tgccgagcag ttggcaacct gggcggagga aaacaatttc
     1801 ttcccagagc atcgcagccc aacccatgac atctttttca actatctgac tcaatacctg
     1861 gaagttcagg ggtgggagca gcaggagcag tcctttcagc ttgaactggc aggctatgtt
     1921 ggcgcaaaga ctaaaactgc cagacaaccg atttgctccc tcagttccgg tgaatttgct
     1981 tcggaagacc agatggattc tgttgtcctg ttcaaaccgc agcagtacag caacaagaat
     2041 cctctcggtg gacggcagat caagcggggg atttccaaaa tctggtccct ggaaatgctg
     2101 ctcaggcagg ctctttggtc tgcgccagca ggcaaactag aggaacaaca acccattttc
     2161 ctttacatct tcccggcata cgtttactcg ccccaggttg ctgctgctgt ccggttactg
     2221 gtgaatgatc tgaagcgtgt caacctctgg gaagtccgta aacactggtt ggagaatggc
     2281 atggatgctc aggcgttgcg atcgtttccc tggctagtcg gtgacaaaaa cgattcgggt
     2341 aactctgctc catccagata ctctggtgaa gacttgcctt tcatggcaat gaactacacc
     2401 accaccagag gcaaaacgat aactgatgcc tgggttgaac ctgctttcct gacactggtg
     2461 ctgcctttgt tgctgggtgt gaaagttgtc gccaccagta gccagattcc actctatgaa
     2521 agcgacaacg acttttgcga atctgtcaag ctggatggac ccgccgattt ctgggctttg
     2581 ctgcggctac caacttccct gcatctccag gattggattg gggggcgtgt tcaaaattta
     2641 gcagaaggct tgaaccgctt gatggttgcc tactgcctgc atctggacag tcgtagcgct
     2701 ccaccagatg cgcgctggag agccttaaac ggcagtgtcc gagagatgac aacaaatgtt
     2761 ctaaacattt tttcacttgc agaggaggga ttacgtcgtg atggctctaa acgcgatgca
     2821 acagttgaag aaattaaccg ctattggaaa tacgcccagc tgtgggcaga gggagactct
     2881 gatatgcaaa acaagttgaa gattaccaaa cgcttagtcg atgagtaccg ccagttttac
     2941 agagccagtg cgaacgaatc cagccatacg gttttgctgc cattttctaa ggcactggaa
     3001 cacattcttt cggtaccaaa tgattgggac gatgaggaaa ttattgttca aggggcagga
     3061 cagttacagg ccgcgttaga tcggcaggaa gtctacaaac gtcccctcat gcttgacaaa
     3121 gagattccct ttgaaattcg tcggatgaag gagtttgagg caattcaaac cttcatgacc
     3181 acctgcgtca ttgacctatt tggacaaatg tgtaaaggcg atcgcgcgct cctgcaggaa
     3241 catcgcaacc gccttaaatc aggggtcgaa tttgcctatc gtcagttagc cctgcaagag
     3301 aaacaagcca aagccaacca aaaatctgga gaacaatcat gagcattctt gaaaccttaa
     3361 agccagagtt ccacacagcc ttccctcgcc tcgcctctgg caaatatgtc catttcttca
     3421 tggtgcgtca cagccagtcg ttccccgtct ttcaaaccga tggcgttttg aacactgccc
     3481 gtactcaggc tgggttaaat gcaggcgatg aaaaatccag acaggcaatc agtcgcctgg
     3541 tcatgttcaa acggaaacag gtgacaccag aacgactcac aggacgggaa ctgctgcgat
     3601 cgctgggcat taacggagta gttgaagggg aagccaattt ctgtgaatac aacggcgaaa
     3661 gatcctgtaa aaaatgccct gattgcattc tctatggttt tgcgattggg gacagtggct
     3721 cggaacgttc caaagtctac tctgattcag ccttttccct gagtccttat gaagtttccc
     3781 atcgcagttt taccttcaat gctccctttg aaggtggaac catgagtgag aagggtgaaa
     3841 tgcggagtgc gatcaacgaa ctggatcatg ttttgccgga agttgctttt ccttgcgttg
     3901 aaacactacg tgaccccacg ctggaaggct tcatttatgt aatgggcaac ctgatccgga
     3961 ctcggcgcta tggagcgcag gagtcgcgta cagggacaat ggcgaatcac attattggcg
     4021 ttaccctatg tgatggcgag atcttcagca atttgcactt tacccaagcc ctctacgatg
     4081 ccctgaaggg tgatttgagt gcgccaatta gtgatttatg cagagaggcg gcgatcgtgg
     4141 cagaaaactt gctaaatcag gaacctgtgc ggaagatcca agttattttt ggagagcagt
     4201 tgaacgaact cctggctgag gtagcgggaa tctgccagga tgaaactcgc ttgcgaaaca
     4261 ctgtgacagc cttgtaccag caatcacaag cctatgccga gacctatggt gctgctgcta
     4321 agaaaggcaa aggcaaatct taaggtggct gccaatggtt atttatcgat gcaatctcac
     4381 gttgcacgac aacgtattct tcgccactcg cgagatgggg attctctacg aaacagagaa
     4441 gtatctacac aactgggcac tcagctttgc tctgtttgaa gccacccaca tccccaaacc
     4501 ctatcgcttg cagggtgcga tcgctcaacg acccggttat ctggaagctg acaacgagca
     4561 aaacctttta gttctgaatc acgcaggcat ctatgttttc cctgctctac cacttcagtg
     4621 gtcctaccaa gtcaatacct tcaaagcggc tcaagtgcct tactatggca aatccaaaca
     4681 gttcggggat aagggagctg atcgcaacta cccgattaac tacggacggg caaaagaact
     4741 ggcagttggc agcacctacc gaacgtatat tcttgcccct gaaccaatcc agatccccca
     4801 ctggatacgg ttgggtaagt gggcagccaa gattcaggtg gacgcaagac ccatacctaa
     4861 ctgtggaatc cgacaacggt caggagaata cacctgcgac catccactaa atccgatcga
     4921 cctgtccggt tcaacccgat tgctgttata caaccgcatt gtcatgccac ccgctagtct
     4981 cgtcagtcag tcgcgagttt ctggggacta ctgggaaatt tctaaaccat ccaattggac
     5041 tgaatttccg gactggtcag acctgaaaga catacccaat caagtttgct tacccagcgg
     5101 agtagcctat ggagccagta ctgccattgc cgcatcttaa gtccaccgct ctgcatatcc
     5161 tggtaattca tttaggcgtt gccgaaagcg gagccttgcc tgttgaatcc ggtcgagccg
     5221 tccatgctca agttttggac tggttccgca tggcggatcc ggcaatttct gaggcaattc
     5281 acaaaagcca ggaatccccg ctcagtctct caggcctggt cgggaaacgc cgtaaagtag
     5341 gggtgcgggc aggagatgaa ttttacttcc gaattgggtt gttagatggg agtttgattg
     5401 aaccactgct gagtggtttg gagaagtggg gcgacaagcc cttttctctg gcaaagtgtc
     5461 cctttgtttt ccgcagtatc aatatgttgc caggaactga cccctgggtt ggttcttccg
     5521 attatgcctt gcttacccaa accccggcta ctttggataa cctagctctc aagctcctgt
     5581 cgccaacaag cttcaagtcg aatgccgggc aaggaatcca accctttcct ttgccagagt
     5641 cagttttcgg aaatttacat cgtcgctgga atgtctttgc accaaaggaa ttgcagtttc
     5701 caaaaattca atggagtggg ctggtctctg attatgacct caaaactcaa aagttaagga
     5761 tggaaaactc ggttgagatc ggcgcagtgg gctgggtgcg ctatcggttt ccagatccag
     5821 aacaagcgag aattgcaacc attctggctc actttgcctt ttttgctgga gttgggcgta
     5881 agacagcaat ggggatggga caggtggtgt tggacaaaaa aacgtagcct aggctgctgc
     5941 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     6001 tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
     6061 accggtaaac cagcaataga cataagcggc tatttaacga ccctcgggat ctcgacgctc
     6121 tcccttatgc gactcctgca ttaggaaatt aatacgactc actatagggg aattgtgagc
     6181 ggataacaat tcccctgtag aaataatttt gtttaacttt aataaggaga tataccatgc
     6241 ttgatgaaaa aagcttcgac aactggtgcc aatccgttgg tctgagccag gcagcacgtg
     6301 aagttgtaga gcgtgtgcga tcctcttcac cctcccgtcg cgtacagagc ggtagaggaa
     6361 acgtctccgg caattaccct agccgcaaaa tgggcgtcac aattcagttt gagtcccatc
     6421 gaaatgagct tgcccgcatc tacgaactgg agcatgaccc cggcgttctg gaatattacg
     6481 atcaacctcc caaaatcgaa cttgattatt tgtcaaaaaa tgggaggcgt gttcgtactc
     6541 aatacacacc tgatttcttt gttatccgta atgactcagc aggttgggag gagtgtaaaa
     6601 cagaggatga ccttaagcaa ttagctgagg acagccctaa ccgttaccaa cgagctgagg
     6661 acaatcgctg gcgatgtcca cctgcagaag cctatgctct tccccttgag ttgtattttc
     6721 aggtacggtc tagtgctgaa attaactgga ttttccagca aaactttgta tggctagaag
     6781 actaccttgg tactgaatca cttactatgg atgagtcggt tgcagatgca atcatagcca
     6841 cagtcagagc aaacgagggt atcacactac tcgaactgat tcaacaatta caaggcacag
     6901 atgctgatgc tatctacatc ctgattgcaa ccgaacaaat ttatgttgat ttgatggctg
     6961 aacggttagc gaacagcgat cgggcacggg tattttccag tccagaaatt gctcaagcct
     7021 ttcaactcat tacgctggtc gaagcacctc tggctaacag ttctctccat atagttcagg
     7081 tgggcgttgg cacaaccata cactgggatg gtgaagtctg ggagattgtt aatacaggct
     7141 ctaccatgac aggattgttg cgatcggacg gtaagtttat cgagttgccc aattctgcaa
     7201 ttgatgaatt gattcagaag ggcaagattc aatcaatttc agcacaacaa gcccctgata
     7261 gcaaagcaga agttgaagaa atgcttcgct gtgctcgtcc agccgacatt aaagaagcca
     7321 accgtcgcta cgaaatcatt cagccttacc tggaaaagga tgcaccagct tgtccaactc
     7381 gaacaattcg gcgctggcgt aatcagtatt ggcaggcaga aaagctttac gggaatggct
     7441 atgttggttt actaccaaat catcagaaga aagggaatta tttagttaag gttaatgaag
     7501 taactcaagc ttttatgctt gagtttattg agaagcatta cgaaaatctc aaacagcgta
     7561 gtaagcttag agtttatcag gcttttgtgg aggcatgtga aagccacgaa ccgaaactaa
     7621 accctccaag ccgtactaca ttcacactag cgattcagaa acgagcagga catcagcaaa
     7681 ccttgaaacg tgaaggacga agagctgcca ttcagaagga ggagttttac tgggaactgg
     7741 agctaactac tcctcgacac ggcgatcgcc ccttcgagat cgttcatatt gatcacactc
     7801 aactcgatat tgagcttatc agttctttga actccctggc ccaatgcaac cctacatctg
     7861 ttgatgccac ttacaatttg ggtcgtcctt gggcaacgtt catggttgat gcttacagcc
     7921 gccgtttgct agcagtttac ataacctatg aagaacccag ctatcgttcc tgcatgatgg
     7981 tgctccgtat ttgcgtacag cgatttaagc gttttcctca gacgatcgtg gtagataatg
     8041 gtgcagaatt tcacagccat tactttgaac aactactggc aacatatcaa tgcacaaaga
     8101 agcacaggcc acctgcaaaa gctcgctttg gtgcagttgt tgaacgatta tttggcactg
     8161 caaataagca attaattcat gagttgcagg gaaataccca aattactcgc aagcatcgcc
     8221 aagttactca atctgttcat ccccaaaaat tggctctttg gacgttaggc gatctctacg
     8281 aatcactatg tgaatgggct tacaaagttt atgctgaccg ccagcaccct gcgctagggc
     8341 aaagccctaa tgctgcattc gctcaagggc tggcgttggg aggtagtcgc atccatcgcc
     8401 gagtagagta tgaccaagtc ttccgtatct tgacattgcc cgcaccggaa agaggacaac
     8461 gcaaagttca gccaggacaa gggataaaac ttagcaatat ctactactgg tcaaatgcct
     8521 ttcgagaccc agaaattgaa caatcgatgg ttgaagttcg ttatgatccc tttgatgcca
     8581 gcattgccta tgcactggtg cgaaaacaat gggtccggtg tatttcctcc tactatcaac
     8641 agtttcaaaa tcgttcagaa aaggaaattc gtttaataac tgctgagttg attaagcaaa
     8701 agcgtgacca gggaagagat acaaatgtta cggataaaga attggcgcag tttttaagct
     8761 ctacagaaat gaaagaaggc aaattcttgg agcaacgctt gcggactaca gaaaacaggg
     8821 tagttattca gaatagtaca gctacagaag acgatccccc catcactaat tctcagaatt
     8881 cttacgagcc atcgcttatg ctgccaagtg aggatgagtt ggatgatcag gaagactggt
     8941 taaataccgg caaagctgag gcagaattag aattctatgg agagtactag cgatgcaacc
     9001 tcaaataagc ttcccaaaag agttactgct tcaaccaatt cctgatcgaa ttgcctattt
     9061 caaagattac acaatggcac atccgaagct tatagaagca gaccggaagt taatgcatgc
     9121 tattcgtgaa cctggaggag ctgctcttat ctttgtcttt ggtcctaccg gagttggaaa
     9181 gtctactcta ttgaaacgaa ttactcaaaa gctgcttaaa gaagcattgt ctttgatgga
     9241 gactgacaaa ggctatattc ctattgcggg ggtagagata aaaactccag aggatagtaa
     9301 ctttgattgg aacgattttt atgttcgtgc tcttatggca cttaatgagc caatgatcga
     9361 gaaaaaaatc aaccgtacag gtgcaaaact aaaattacgg ttggcacttg aatctgccct
     9421 tcgtaatcgc cgtcctgatg ctttttacat agacgaagct caaaattttg gtaaggtagc
     9481 cagtgggcga aaattgcaag atcaaacaga ttgcattaaa tctcttgcaa atctcgctca
     9541 aactcggttt atcttatgtg ggatttatga acttttacca ctacgaaatc taagtgggca
     9601 gctatgccgc cgtagccata gtattcactt tccacgttat tcggcagatt ctgagcagga
     9661 tctaaaggcc tttaaaagtg cgttattaac actacaaaat caactccctc ttcccgaacc
     9721 acctgattta gtggcgcatt gggaattttg ttatgagaga actatcggct gtattggtat
     9781 tttgaaagat ttgctccttc ggactctaag cgcgatttta gtacgggatg gagacaaagt
     9841 tttgacattg aagcccaagg atttagaaca acgtgcatgg tctttagaag aatgtttggt
     9901 tatgctgaga gaggcaaagg aaggagagca acaacttact gacaaatcgg gcttacatga
     9961 ggaattacgg acagctttag ggtttaatgc agctacgtcc aagcagtctg ccaaatcatc
    10021 caagtcaaca tcaagcaaaa aagcaggcag tgttggaaag ccttcacctc gtcgtcgttc
    10081 agttggaata gatcaagatg ccagctaacg atctagaaat ctatgaatct tggaatttag
    10141 atctcccttt actacctaat cgaagtaggc tctaccactt gaaaccgatt gcaattggag
    10201 atgcttacgt tgaaggatta atcagctatg tttgccgttt ggctgaagcc cactgtgttt
    10261 ctccaggagt ccttactaaa caggaaatat tgcccagtct aagaaaagtt tataatgtgg
    10321 gttcaagaga agttcatgaa gtacgaagag atgaaagttt agtttctatt tcctcctttc
    10381 ctaagccaga aagcaataaa aatcctaatg aaccaggctt ctgggcatgg caatatattg
    10441 aaggattgca gcctttaact cttagagaaa accttgaagc attaactatt ccaaattgga
    10501 aaaagcaaat tccacaggtg acaggaattg aggttgcgcg tgagttgaga gcttggtgcc
    10561 ctgaatgctt tcaacaatgg cgcaagacta atcagataat ttatgaaccg atgctttggt
    10621 cgatcgcttt agttaccgta tgtccttatc attaccttcc gctacaaact cgctgtcctt
    10681 attgtaaaaa aactcaacgg cctatcacag gaaaaatgca ggtagctcgc tgttccgaat
    10741 gtttagagtg gcttggcatt caaccaagcg aagattcaga gttagaaatc aaaattaaat
    10801 ctgaagtcga atggcaccat tgggttgcgg agaaagtaat ggagattttg gtatttaaag
    10861 attatacttt aactgcaaat gctataacaa tagcagctcc aattacgaaa gaaaaatatt
    10921 ttcaactaga atcgagctta ataaattgtc caattaagta tgctcaacag cagttcttaa
    10981 agctcttctt caattaaatt tataaaggta tggctatggc gactgaagta ccttttactt
    11041 ttagcacgtg ggatttatct aaacccctta ccccagctcg tagttctctc tttagcttga
    11101 aaccgattgg ggttggaacg gctgcagtag aaagcctcac tggatatatt gcacgtttgg
    11161 ctgaagctca ctgtatctgg ccaggagatc ttcttgcagc tgaatttagt tcttcatatt
    11221 cctattctta cctgcatagc atgaggggcc gtattgctgc tttgaatggt aatagccaaa
    11281 tggctcagga tgctgtactt aagcttcaag agcttacctt acaggagaat ttatccgctt
    11341 taacaatgtt aggctgggct aatattttac ccgcaaaagg gttgttaaat cggactcgaa
    11401 cttggtgtcc ggtttgtttt gaggcgtggc gtttcatcaa aagtcctata tatgagcctc
    11461 tattatggta tttaacctcg gtcaaatatt gtccaattca tcagcaacgc cttttatctc
    11521 aatgtccatt ctgcgggtgc cagcagcctc ctctagactg gaaaactcgg ccgggctatt
    11581 gttcaaggtg ccaggtgtgg ctgggttcac caccaggtgt aacttccagt aattcgcttt
    11641 ctaatacaga aataaggtgg gagagttggg ttgcccagag ctgcggaaat ttgttagcct
    11701 atagtcctcg tttacacaag atatccgtta gcgttcaggt cgcagcatcc ctttctaaat
    11761 gtatcaacct ttatgcagag ggcaatattg cggagtttgc ccgtcaaatg agcttgccaa
    11821 aaacttctgt ttggctgtgg cagtctggtc aggtactccc cgagttatcc aggcttttaa
    11881 gcatctgcta tcacttcaat ctgtcgctct tagactttct aattaataca ccagttctta
    11941 agataaggag agacttaatt ccagaaaaga gaactaatga gtttcgccag cctcgaagag
    12001 atccctcaac catatctcaa ttgcaaccta ttctgggatt aattcttttc gagcagccac
    12061 ctccctcttt agcagcagta gcgtgtcgct taggttgtag ctcaagatcc ctgcgccata
    12121 attttcctga tctctgtggc tctatttctg cacgttacct tcaacaccgt gatgaagttt
    12181 ataaagaaaa attgaatcaa ttctgtcaag acttgcgcca agttgtactt caactttatt
    12241 cggcaggcat tgatccgact cggagccaag ttgctcgccg tctgaggaaa cccgcttatt
    12301 ttagagaacc cgaagtcgag gcaatgcttg agtctgttcg acgagaatta ggcttgcaca
    12361 gcaattctca gcatgagact aattctcaca aaaacgccca gttttaactt aagtcgaaca
    12421 gaaagtaatc gtattgtaca cggccgcata atcgaaatta atacgactca ctatagggga
    12481 attgtgagcg gataacaatt ccccatctta gtatattagt taagtataag aaggagatat
    12541 acatatggca gatctcaatt ggatatcggc cggccacgcg atcgctgacg tcggtaccct
    12601 cgagtctggt aaagaaaccg ctgctgcgaa atttgaacgc cagcacatgg actcgtctac
    12661 tagcgcagct taattaacct aggctgctgc caccgctgag caataactag cataacccct
    12721 tggggcctct aaacgggtct tgaggggttt tttgctgaaa cctcaggcat ttgagaagca
    12781 cacggtcaca ctgcttccgg tagtgccctg aaccgacgac cgggtcatcg tggccggatc
    12841 ttgcggcccc tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct
    12901 cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt
    12961 cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca
    13021 ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc
    13081 tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg
    13141 gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg
    13201 gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg
    13261 tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt
    13321 cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga
    13381 tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg
    13441 aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta
    13501 cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg
    13561 agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta
    13621 cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatactc ttcctttttc
    13681 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    13741 tttagaaaaa taaacaaata gctagctcac tcggtcgcta cgctccgggc gtgagactgc
    13801 ggcgggcgct gcggacacat acaaagttac ccacagattc cgtggataag caggggacta
    13861 acatgtgagg caaaacagca gggccgcgcc ggtggcgttt ttccataggc tccgccctcc
    13921 tgccagagtt cacataaaca gacgcttttc cggtgcatct gtgggagccg tgaggctcaa
    13981 ccatgaatct gacagtacgg gcgaaacccg acaggactta aagatcccca ccgtttccgg
    14041 cgggtcgctc cctcttgcgc tctcctgttc cgaccctgcc gtttaccgga tacctgttcc
    14101 gcctttctcc cttacgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc
    14161 tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac
    14221 tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cggaaaagca cggtaaaacg
    14281 ccactggcag cagccattgg taactgggag ttcgcagagg atttgtttag ctaaacacgc
    14341 ggttgctctt gaagtgtgcg ccaaagtccg gctacactgg aaggacagat ttggttgctg
    14401 tgctctgcga aagccagtta ccacggttaa gcagttcccc aactgactta accttcgatc
    14461 aaaccacctc cccaggtggt tttttcgttt acagggcaaa agattacgcg cagaaaaaaa
    14521 ggatctcaag aagatccttt gatcttttct actgaaccgc tctagatttc agtgcaattt
    14581 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
    14641 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
    14701 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
    14761 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    14821 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
    14881 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
    14941 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
    15001 ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg cagcccggac
    15061 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
    15121 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
    15181 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
    15241 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
    15301 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
    15361 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
    15421 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
    15481 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
    15541 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
    15601 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
    15661 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
    15721 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
    15781 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
    15841 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
    15901 cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaaatt
    15961 aatacgactc actata
//
