LOCUS       sequence-397087-       15194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of CyCAST proteins (except TniQ) with crRNA 
            targeting CyPSP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15194)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 15194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pMSIP267: pHelper(CyCAST)_PSP1_Delta-TniQ
FEATURES             Location/Qualifiers
     source          1..15194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        290..308
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    309..333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      5957..6004
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6150..6168
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6169..6193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        11677..11695
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    11696..11720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             11829..11873
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      11925..11972
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(12093..12884)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(12885..12976)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13024..13762)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(13972..15054)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     15016..15035
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(15055..15132)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(15178..15194,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atggtttcca agcctgaatc ccggtctacg ggactgaaac gagaagtcat
      121 ttaataaggc cactgttaaa cgtaggtttc caagcctgaa tcccggtcta cgggactgaa
      181 acgatccgaa ttcgagctcg gcgcgcctgc aggtcgacaa gcttgcggcc gcataatgct
      241 taagtcgaac agaaagtaat cgtattgtac acggccgcat aatcgaaatt aatacgactc
      301 actatagggg aattgtgagc ggataacaat tccccatctt agtatattag ttaagtataa
      361 gaaggagata tacatatggc taccctactc caaaccctcc tgttacgaac gttgtcaccc
      421 gaggcggatc cagttctcta tgtctacatt gagacggtac tacctgcaat ggaacgggaa
      481 tttggcttga ttccagccct tggtggctcg gaggaagcac actttcatct gctgaggcga
      541 cggggaaaca aatatgcggc ggaaaattcc aagcggtacg ccagtcgctc tgatcaaagc
      601 ctgctggttc atgtgctcaa tgccttgctg accgcctgga atttgttgcc attcctaagc
      661 tccacatttg aactatcaga tgatgagaaa cggctgctct gcctggggct gaccctacac
      721 gattacaaca agtggtgcca gggtgaggag gaagattcgc cgattgcatg cgaagtagca
      781 gagattcttg agctttgtca aacattggga gagcggttga actttgacgg attctgggca
      841 gagtggcggg agtatttgac agagattgcc taccttgccc aaaacaccca gggaaaagtt
      901 ggaacaaatc tacccaaagc gaattggggc agcttcaaaa ttctagatac ccgtcgtctg
      961 gattcgcccc tacgatggct actcgctttt ggcgatgtcg cagtccatct cagcgatcca
     1021 tctgacatcg caatgaaaac ggggggagat cgcctgcggg aacatctgcg ctttctaggt
     1081 attcgtcgca agctggtcta ccatcgcctg cgcaactgta cagggctatt aaccaacgga
     1141 attcataatg cggtaatgca ttttgctaga gaactggatt ggcaacccat cctcttcttt
     1201 gctcagggtg ttgtttatct cgctcctcag ggtgctggcg ttccggatcg cttagaactt
     1261 caggaatttc tctgggagca ggtcagcaac cgattagcca ccagcatgtt gcggggagag
     1321 gttggtttca aacgggatgg caagggactc aaggtggctc cccaaaccct ggaactgttc
     1381 tcccctgctc agttgattcg gaacttgccc ggtgttgtcc aatcttatgt cggaaacgct
     1441 aaatctcctg cgactccaaa acgacttgag aagctggaac tcagttcaac agagcgagag
     1501 tttttggcta aaggtgcaga tttaagagca gatcgcattg ccgagtttat ctttctggcg
     1561 caaaaagagt ttttcggaga taaaccagaa tttatcactt ggatgcttga aactctgggg
     1621 gttcaaactg ctatcacttc ggagcagacg cagcttcaat ccggtggtgt gaactgggga
     1681 tggtatcacg cagcagccta ttacgtcgcg agcaatgcca cgctggattt ggaagagatt
     1741 tcggagaaac ttcaggaatt tgccgagcag ttggcaacct gggcggagga aaacaatttc
     1801 ttcccagagc atcgcagccc aacccatgac atctttttca actatctgac tcaatacctg
     1861 gaagttcagg ggtgggagca gcaggagcag tcctttcagc ttgaactggc aggctatgtt
     1921 ggcgcaaaga ctaaaactgc cagacaaccg atttgctccc tcagttccgg tgaatttgct
     1981 tcggaagacc agatggattc tgttgtcctg ttcaaaccgc agcagtacag caacaagaat
     2041 cctctcggtg gacggcagat caagcggggg atttccaaaa tctggtccct ggaaatgctg
     2101 ctcaggcagg ctctttggtc tgcgccagca ggcaaactag aggaacaaca acccattttc
     2161 ctttacatct tcccggcata cgtttactcg ccccaggttg ctgctgctgt ccggttactg
     2221 gtgaatgatc tgaagcgtgt caacctctgg gaagtccgta aacactggtt ggagaatggc
     2281 atggatgctc aggcgttgcg atcgtttccc tggctagtcg gtgacaaaaa cgattcgggt
     2341 aactctgctc catccagata ctctggtgaa gacttgcctt tcatggcaat gaactacacc
     2401 accaccagag gcaaaacgat aactgatgcc tgggttgaac ctgctttcct gacactggtg
     2461 ctgcctttgt tgctgggtgt gaaagttgtc gccaccagta gccagattcc actctatgaa
     2521 agcgacaacg acttttgcga atctgtcaag ctggatggac ccgccgattt ctgggctttg
     2581 ctgcggctac caacttccct gcatctccag gattggattg gggggcgtgt tcaaaattta
     2641 gcagaaggct tgaaccgctt gatggttgcc tactgcctgc atctggacag tcgtagcgct
     2701 ccaccagatg cgcgctggag agccttaaac ggcagtgtcc gagagatgac aacaaatgtt
     2761 ctaaacattt tttcacttgc agaggaggga ttacgtcgtg atggctctaa acgcgatgca
     2821 acagttgaag aaattaaccg ctattggaaa tacgcccagc tgtgggcaga gggagactct
     2881 gatatgcaaa acaagttgaa gattaccaaa cgcttagtcg atgagtaccg ccagttttac
     2941 agagccagtg cgaacgaatc cagccatacg gttttgctgc cattttctaa ggcactggaa
     3001 cacattcttt cggtaccaaa tgattgggac gatgaggaaa ttattgttca aggggcagga
     3061 cagttacagg ccgcgttaga tcggcaggaa gtctacaaac gtcccctcat gcttgacaaa
     3121 gagattccct ttgaaattcg tcggatgaag gagtttgagg caattcaaac cttcatgacc
     3181 acctgcgtca ttgacctatt tggacaaatg tgtaaaggcg atcgcgcgct cctgcaggaa
     3241 catcgcaacc gccttaaatc aggggtcgaa tttgcctatc gtcagttagc cctgcaagag
     3301 aaacaagcca aagccaacca aaaatctgga gaacaatcat gagcattctt gaaaccttaa
     3361 agccagagtt ccacacagcc ttccctcgcc tcgcctctgg caaatatgtc catttcttca
     3421 tggtgcgtca cagccagtcg ttccccgtct ttcaaaccga tggcgttttg aacactgccc
     3481 gtactcaggc tgggttaaat gcaggcgatg aaaaatccag acaggcaatc agtcgcctgg
     3541 tcatgttcaa acggaaacag gtgacaccag aacgactcac aggacgggaa ctgctgcgat
     3601 cgctgggcat taacggagta gttgaagggg aagccaattt ctgtgaatac aacggcgaaa
     3661 gatcctgtaa aaaatgccct gattgcattc tctatggttt tgcgattggg gacagtggct
     3721 cggaacgttc caaagtctac tctgattcag ccttttccct gagtccttat gaagtttccc
     3781 atcgcagttt taccttcaat gctccctttg aaggtggaac catgagtgag aagggtgaaa
     3841 tgcggagtgc gatcaacgaa ctggatcatg ttttgccgga agttgctttt ccttgcgttg
     3901 aaacactacg tgaccccacg ctggaaggct tcatttatgt aatgggcaac ctgatccgga
     3961 ctcggcgcta tggagcgcag gagtcgcgta cagggacaat ggcgaatcac attattggcg
     4021 ttaccctatg tgatggcgag atcttcagca atttgcactt tacccaagcc ctctacgatg
     4081 ccctgaaggg tgatttgagt gcgccaatta gtgatttatg cagagaggcg gcgatcgtgg
     4141 cagaaaactt gctaaatcag gaacctgtgc ggaagatcca agttattttt ggagagcagt
     4201 tgaacgaact cctggctgag gtagcgggaa tctgccagga tgaaactcgc ttgcgaaaca
     4261 ctgtgacagc cttgtaccag caatcacaag cctatgccga gacctatggt gctgctgcta
     4321 agaaaggcaa aggcaaatct taaggtggct gccaatggtt atttatcgat gcaatctcac
     4381 gttgcacgac aacgtattct tcgccactcg cgagatgggg attctctacg aaacagagaa
     4441 gtatctacac aactgggcac tcagctttgc tctgtttgaa gccacccaca tccccaaacc
     4501 ctatcgcttg cagggtgcga tcgctcaacg acccggttat ctggaagctg acaacgagca
     4561 aaacctttta gttctgaatc acgcaggcat ctatgttttc cctgctctac cacttcagtg
     4621 gtcctaccaa gtcaatacct tcaaagcggc tcaagtgcct tactatggca aatccaaaca
     4681 gttcggggat aagggagctg atcgcaacta cccgattaac tacggacggg caaaagaact
     4741 ggcagttggc agcacctacc gaacgtatat tcttgcccct gaaccaatcc agatccccca
     4801 ctggatacgg ttgggtaagt gggcagccaa gattcaggtg gacgcaagac ccatacctaa
     4861 ctgtggaatc cgacaacggt caggagaata cacctgcgac catccactaa atccgatcga
     4921 cctgtccggt tcaacccgat tgctgttata caaccgcatt gtcatgccac ccgctagtct
     4981 cgtcagtcag tcgcgagttt ctggggacta ctgggaaatt tctaaaccat ccaattggac
     5041 tgaatttccg gactggtcag acctgaaaga catacccaat caagtttgct tacccagcgg
     5101 agtagcctat ggagccagta ctgccattgc cgcatcttaa gtccaccgct ctgcatatcc
     5161 tggtaattca tttaggcgtt gccgaaagcg gagccttgcc tgttgaatcc ggtcgagccg
     5221 tccatgctca agttttggac tggttccgca tggcggatcc ggcaatttct gaggcaattc
     5281 acaaaagcca ggaatccccg ctcagtctct caggcctggt cgggaaacgc cgtaaagtag
     5341 gggtgcgggc aggagatgaa ttttacttcc gaattgggtt gttagatggg agtttgattg
     5401 aaccactgct gagtggtttg gagaagtggg gcgacaagcc cttttctctg gcaaagtgtc
     5461 cctttgtttt ccgcagtatc aatatgttgc caggaactga cccctgggtt ggttcttccg
     5521 attatgcctt gcttacccaa accccggcta ctttggataa cctagctctc aagctcctgt
     5581 cgccaacaag cttcaagtcg aatgccgggc aaggaatcca accctttcct ttgccagagt
     5641 cagttttcgg aaatttacat cgtcgctgga atgtctttgc accaaaggaa ttgcagtttc
     5701 caaaaattca atggagtggg ctggtctctg attatgacct caaaactcaa aagttaagga
     5761 tggaaaactc ggttgagatc ggcgcagtgg gctgggtgcg ctatcggttt ccagatccag
     5821 aacaagcgag aattgcaacc attctggctc actttgcctt ttttgctgga gttgggcgta
     5881 agacagcaat ggggatggga caggtggtgt tggacaaaaa aacgtagcct aggctgctgc
     5941 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     6001 tttgctgaaa cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
     6061 accggtaaac cagcaataga cataagcggc tatttaacga ccctcgggat ctcgacgctc
     6121 tcccttatgc gactcctgca ttaggaaatt aatacgactc actatagggg aattgtgagc
     6181 ggataacaat tcccctgtag aaataatttt gtttaacttt aataaggaga tataccatgc
     6241 ttgatgaaaa aagcttcgac aactggtgcc aatccgttgg tctgagccag gcagcacgtg
     6301 aagttgtaga gcgtgtgcga tcctcttcac cctcccgtcg cgtacagagc ggtagaggaa
     6361 acgtctccgg caattaccct agccgcaaaa tgggcgtcac aattcagttt gagtcccatc
     6421 gaaatgagct tgcccgcatc tacgaactgg agcatgaccc cggcgttctg gaatattacg
     6481 atcaacctcc caaaatcgaa cttgattatt tgtcaaaaaa tgggaggcgt gttcgtactc
     6541 aatacacacc tgatttcttt gttatccgta atgactcagc aggttgggag gagtgtaaaa
     6601 cagaggatga ccttaagcaa ttagctgagg acagccctaa ccgttaccaa cgagctgagg
     6661 acaatcgctg gcgatgtcca cctgcagaag cctatgctct tccccttgag ttgtattttc
     6721 aggtacggtc tagtgctgaa attaactgga ttttccagca aaactttgta tggctagaag
     6781 actaccttgg tactgaatca cttactatgg atgagtcggt tgcagatgca atcatagcca
     6841 cagtcagagc aaacgagggt atcacactac tcgaactgat tcaacaatta caaggcacag
     6901 atgctgatgc tatctacatc ctgattgcaa ccgaacaaat ttatgttgat ttgatggctg
     6961 aacggttagc gaacagcgat cgggcacggg tattttccag tccagaaatt gctcaagcct
     7021 ttcaactcat tacgctggtc gaagcacctc tggctaacag ttctctccat atagttcagg
     7081 tgggcgttgg cacaaccata cactgggatg gtgaagtctg ggagattgtt aatacaggct
     7141 ctaccatgac aggattgttg cgatcggacg gtaagtttat cgagttgccc aattctgcaa
     7201 ttgatgaatt gattcagaag ggcaagattc aatcaatttc agcacaacaa gcccctgata
     7261 gcaaagcaga agttgaagaa atgcttcgct gtgctcgtcc agccgacatt aaagaagcca
     7321 accgtcgcta cgaaatcatt cagccttacc tggaaaagga tgcaccagct tgtccaactc
     7381 gaacaattcg gcgctggcgt aatcagtatt ggcaggcaga aaagctttac gggaatggct
     7441 atgttggttt actaccaaat catcagaaga aagggaatta tttagttaag gttaatgaag
     7501 taactcaagc ttttatgctt gagtttattg agaagcatta cgaaaatctc aaacagcgta
     7561 gtaagcttag agtttatcag gcttttgtgg aggcatgtga aagccacgaa ccgaaactaa
     7621 accctccaag ccgtactaca ttcacactag cgattcagaa acgagcagga catcagcaaa
     7681 ccttgaaacg tgaaggacga agagctgcca ttcagaagga ggagttttac tgggaactgg
     7741 agctaactac tcctcgacac ggcgatcgcc ccttcgagat cgttcatatt gatcacactc
     7801 aactcgatat tgagcttatc agttctttga actccctggc ccaatgcaac cctacatctg
     7861 ttgatgccac ttacaatttg ggtcgtcctt gggcaacgtt catggttgat gcttacagcc
     7921 gccgtttgct agcagtttac ataacctatg aagaacccag ctatcgttcc tgcatgatgg
     7981 tgctccgtat ttgcgtacag cgatttaagc gttttcctca gacgatcgtg gtagataatg
     8041 gtgcagaatt tcacagccat tactttgaac aactactggc aacatatcaa tgcacaaaga
     8101 agcacaggcc acctgcaaaa gctcgctttg gtgcagttgt tgaacgatta tttggcactg
     8161 caaataagca attaattcat gagttgcagg gaaataccca aattactcgc aagcatcgcc
     8221 aagttactca atctgttcat ccccaaaaat tggctctttg gacgttaggc gatctctacg
     8281 aatcactatg tgaatgggct tacaaagttt atgctgaccg ccagcaccct gcgctagggc
     8341 aaagccctaa tgctgcattc gctcaagggc tggcgttggg aggtagtcgc atccatcgcc
     8401 gagtagagta tgaccaagtc ttccgtatct tgacattgcc cgcaccggaa agaggacaac
     8461 gcaaagttca gccaggacaa gggataaaac ttagcaatat ctactactgg tcaaatgcct
     8521 ttcgagaccc agaaattgaa caatcgatgg ttgaagttcg ttatgatccc tttgatgcca
     8581 gcattgccta tgcactggtg cgaaaacaat gggtccggtg tatttcctcc tactatcaac
     8641 agtttcaaaa tcgttcagaa aaggaaattc gtttaataac tgctgagttg attaagcaaa
     8701 agcgtgacca gggaagagat acaaatgtta cggataaaga attggcgcag tttttaagct
     8761 ctacagaaat gaaagaaggc aaattcttgg agcaacgctt gcggactaca gaaaacaggg
     8821 tagttattca gaatagtaca gctacagaag acgatccccc catcactaat tctcagaatt
     8881 cttacgagcc atcgcttatg ctgccaagtg aggatgagtt ggatgatcag gaagactggt
     8941 taaataccgg caaagctgag gcagaattag aattctatgg agagtactag cgatgcaacc
     9001 tcaaataagc ttcccaaaag agttactgct tcaaccaatt cctgatcgaa ttgcctattt
     9061 caaagattac acaatggcac atccgaagct tatagaagca gaccggaagt taatgcatgc
     9121 tattcgtgaa cctggaggag ctgctcttat ctttgtcttt ggtcctaccg gagttggaaa
     9181 gtctactcta ttgaaacgaa ttactcaaaa gctgcttaaa gaagcattgt ctttgatgga
     9241 gactgacaaa ggctatattc ctattgcggg ggtagagata aaaactccag aggatagtaa
     9301 ctttgattgg aacgattttt atgttcgtgc tcttatggca cttaatgagc caatgatcga
     9361 gaaaaaaatc aaccgtacag gtgcaaaact aaaattacgg ttggcacttg aatctgccct
     9421 tcgtaatcgc cgtcctgatg ctttttacat agacgaagct caaaattttg gtaaggtagc
     9481 cagtgggcga aaattgcaag atcaaacaga ttgcattaaa tctcttgcaa atctcgctca
     9541 aactcggttt atcttatgtg ggatttatga acttttacca ctacgaaatc taagtgggca
     9601 gctatgccgc cgtagccata gtattcactt tccacgttat tcggcagatt ctgagcagga
     9661 tctaaaggcc tttaaaagtg cgttattaac actacaaaat caactccctc ttcccgaacc
     9721 acctgattta gtggcgcatt gggaattttg ttatgagaga actatcggct gtattggtat
     9781 tttgaaagat ttgctccttc ggactctaag cgcgatttta gtacgggatg gagacaaagt
     9841 tttgacattg aagcccaagg atttagaaca acgtgcatgg tctttagaag aatgtttggt
     9901 tatgctgaga gaggcaaagg aaggagagca acaacttact gacaaatcgg gcttacatga
     9961 ggaattacgg acagctttag ggtttaatgc agctacgtcc aagcagtctg ccaaatcatc
    10021 caagtcaaca tcaagcaaaa aagcaggcag tgttggaaag ccttcacctc gtcgtcgttc
    10081 agttggaata gatcaagatg ccagctaata gcagctccaa ttacgaaaga aaaatatttt
    10141 caactagaat cgagcttaat aaattgtcca attaagtatg ctcaacagca gttcttaaag
    10201 ctcttcttca attaaattta taaaggtatg gctatggcga ctgaagtacc ttttactttt
    10261 agcacgtggg atttatctaa accccttacc ccagctcgta gttctctctt tagcttgaaa
    10321 ccgattgggg ttggaacggc tgcagtagaa agcctcactg gatatattgc acgtttggct
    10381 gaagctcact gtatctggcc aggagatctt cttgcagctg aatttagttc ttcatattcc
    10441 tattcttacc tgcatagcat gaggggccgt attgctgctt tgaatggtaa tagccaaatg
    10501 gctcaggatg ctgtacttaa gcttcaagag cttaccttac aggagaattt atccgcttta
    10561 acaatgttag gctgggctaa tattttaccc gcaaaagggt tgttaaatcg gactcgaact
    10621 tggtgtccgg tttgttttga ggcgtggcgt ttcatcaaaa gtcctatata tgagcctcta
    10681 ttatggtatt taacctcggt caaatattgt ccaattcatc agcaacgcct tttatctcaa
    10741 tgtccattct gcgggtgcca gcagcctcct ctagactgga aaactcggcc gggctattgt
    10801 tcaaggtgcc aggtgtggct gggttcacca ccaggtgtaa cttccagtaa ttcgctttct
    10861 aatacagaaa taaggtggga gagttgggtt gcccagagct gcggaaattt gttagcctat
    10921 agtcctcgtt tacacaagat atccgttagc gttcaggtcg cagcatccct ttctaaatgt
    10981 atcaaccttt atgcagaggg caatattgcg gagtttgccc gtcaaatgag cttgccaaaa
    11041 acttctgttt ggctgtggca gtctggtcag gtactccccg agttatccag gcttttaagc
    11101 atctgctatc acttcaatct gtcgctctta gactttctaa ttaatacacc agttcttaag
    11161 ataaggagag acttaattcc agaaaagaga actaatgagt ttcgccagcc tcgaagagat
    11221 ccctcaacca tatctcaatt gcaacctatt ctgggattaa ttcttttcga gcagccacct
    11281 ccctctttag cagcagtagc gtgtcgctta ggttgtagct caagatccct gcgccataat
    11341 tttcctgatc tctgtggctc tatttctgca cgttaccttc aacaccgtga tgaagtttat
    11401 aaagaaaaat tgaatcaatt ctgtcaagac ttgcgccaag ttgtacttca actttattcg
    11461 gcaggcattg atccgactcg gagccaagtt gctcgccgtc tgaggaaacc cgcttatttt
    11521 agagaacccg aagtcgaggc aatgcttgag tctgttcgac gagaattagg cttgcacagc
    11581 aattctcagc atgagactaa ttctcacaaa aacgcccagt tttaacttaa gtcgaacaga
    11641 aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact ataggggaat
    11701 tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa ggagatatac
    11761 atatggcaga tctcaattgg atatcggccg gccacgcgat cgctgacgtc ggtaccctcg
    11821 agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca gcacatggac tcgtctacta
    11881 gcgcagctta attaacctag gctgctgcca ccgctgagca ataactagca taaccccttg
    11941 gggcctctaa acgggtcttg aggggttttt tgctgaaacc tcaggcattt gagaagcaca
    12001 cggtcacact gcttccggta gtgccctgaa ccgacgaccg ggtcatcgtg gccggatctt
    12061 gcggcccctc ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg
    12121 cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct
    12181 tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg
    12241 cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg
    12301 taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc
    12361 gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga
    12421 tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc
    12481 agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca
    12541 ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg
    12601 tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa
    12661 gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg
    12721 gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag
    12781 ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc
    12841 tctgatagtt gagtcgatac ttcggcgatc accgcttccc tcatactctt cctttttcaa
    12901 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    12961 tagaaaaata aacaaatagc tagctcactc ggtcgctacg ctccgggcgt gagactgcgg
    13021 cgggcgctgc ggacacatac aaagttaccc acagattccg tggataagca ggggactaac
    13081 atgtgaggca aaacagcagg gccgcgccgg tggcgttttt ccataggctc cgccctcctg
    13141 ccagagttca cataaacaga cgcttttccg gtgcatctgt gggagccgtg aggctcaacc
    13201 atgaatctga cagtacgggc gaaacccgac aggacttaaa gatccccacc gtttccggcg
    13261 ggtcgctccc tcttgcgctc tcctgttccg accctgccgt ttaccggata cctgttccgc
    13321 ctttctccct tacgggaagt gtggcgcttt ctcatagctc acacactggt atctcggctc
    13381 ggtgtaggtc gttcgctcca agctgggctg taagcaagaa ctccccgttc agcccgactg
    13441 ctgcgcctta tccggtaact gttcacttga gtccaacccg gaaaagcacg gtaaaacgcc
    13501 actggcagca gccattggta actgggagtt cgcagaggat ttgtttagct aaacacgcgg
    13561 ttgctcttga agtgtgcgcc aaagtccggc tacactggaa ggacagattt ggttgctgtg
    13621 ctctgcgaaa gccagttacc acggttaagc agttccccaa ctgacttaac cttcgatcaa
    13681 accacctccc caggtggttt tttcgtttac agggcaaaag attacgcgca gaaaaaaagg
    13741 atctcaagaa gatcctttga tcttttctac tgaaccgctc tagatttcag tgcaatttat
    13801 ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgtta
    13861 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
    13921 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
    13981 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    14041 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
    14101 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
    14161 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
    14221 gtcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc
    14281 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
    14341 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
    14401 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
    14461 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
    14521 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
    14581 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
    14641 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
    14701 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
    14761 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
    14821 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
    14881 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
    14941 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
    15001 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
    15061 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
    15121 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaaattaa
    15181 tacgactcac tata
//
