LOCUS       sequence-397091-        7120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT 
            in AAV production vector expressed under the mammalian promoter 
            (EF1a), FLP recombinase-dependent.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Lu X, Wang Y, Liu Z, Gou Y, Jaeger D, St-Pierre F
  TITLE     Widefield imaging of rapid pan-cortical voltage dynamics with an 
            indicator evolved for one-photon microscopy.
  JOURNAL   Nat Commun. 2023 Oct 12;14(1):6423. doi: 10.1038/s41467-023-41975-3.
  PUBMED    37828037
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-12"; volume: "14(1):6423. doi"; pages: " 
            10.1038/s41467-023-41975-3"
COMMENT     Sequence Label: pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE
FEATURES             Location/Qualifiers
     source          1..7120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        231..1409
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          462..1400
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1357..1377
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1500..1533
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(3187..3220)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    3247..3835
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3300..3320)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3718..3729)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3867..4343
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4066..4085)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4383..4523
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4383..4512
                     /label=AAV2 ITR
     rep_origin      4598..5053
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4685..4704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4895..4916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5070..5089)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5189..5211
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5249..5267)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5335..5439
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5440..5508,5509..6300)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5658..5677)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6471..7059
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6960..6979
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga
      181 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      241 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      301 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      361 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      421 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      481 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      541 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      601 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      661 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      721 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      781 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      841 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
      901 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
      961 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1021 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1081 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1141 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1201 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1261 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1321 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1381 gtttttttct tccatttcag gtgtcgtgag gtaccgaagt tcctattcta tcagaagtat
     1441 aggaacttcg cagaatggta gctggattgt agctgctatt agcaatatga aacctcttag
     1501 aagttcctat tctctagaaa gtataggaac ttcgaattct tacttttatc aaaagcgggt
     1561 cgcttccgga aaatcggtcg cgcagctaat aaagctatca atgctgctca tgctgcgcat
     1621 atcaatcacg ccttcggtgc gcgccggcag cggcgccacc gggctcggca tgctgctcat
     1681 ttccagttct tccggcggtt tgctctgcgg cgccatttct ttggtgttca gaatcggctg
     1741 gctctgacgc gtggaagatc cagatgaacc tgaagaaccc ttaagggtta ccacttcaag
     1801 ttgtttcttc tgtgaagcca ggcgaaatat tcttattaag ataacaattt tcagaactcg
     1861 aagaagagta accatgtgcg gcatctgatc tgtgttatat tccagtttat gccccagaat
     1921 gttgccatct tctttaaaat cggtgccttt cagttcaatg cggttcacca gggtatcgcc
     1981 ttcaaatttc accaccgcgc gggttttata tttgccatca tctttaaagc taatggtgcg
     2041 ttcctgcaca tagccttccg gcatcgcgct tttaaaaaaa tcatggcgtt tcatatgatc
     2101 cggatagcgg ctaaagcact gcacgccata ggttaaggtg gtcaccaggg tcggccacgg
     2161 caccggtagt ttgccggtgg tgcaaataaa tttcagggtc agtttgccaa tggtcgcatc
     2221 gccttcgcct tcgccgcgca cgctaaattt atggccgttc acatcgccat ccagttccac
     2281 cagaatcggc accacgccgg taaacagttc ttcgccctgg ctcgcgctgc cgccggtgcc
     2341 gccatacagt tcatccatgc cgtgtgtaat gcccgctgcg gtcacaaatt ccagcagcac
     2401 catgtgatcg cgtttttcgt tcggatcttt gctcagcacg gtctgggtgc tcagataatg
     2461 gttatccggc agcagcaccg ggccatcgcc aatcggggtg ttctgctgat aatgatccgc
     2521 cagctgcacg ctgccatctt ccacgttatg gcgcacggta aagttcgctt taatgccgtt
     2581 tttctgttta tccgcggtaa tatacacgtt ctcgctgtta aaggcagcaa ggtcagagaa
     2641 ggagtaggtc atattaatta gcagagtgcc cactactatg actgcatcca aagtaaccag
     2701 tttggaccgg aaatagttct tgaagccttc aacaaacact ctcatgagaa catcaacaag
     2761 gaagaagagt gctatagcca gggaaacacc ttcaagaatc tctctaatgc ctcttttctt
     2821 ctcactgatg gccagatcca caatcaccac tatgatgtct acaatgataa gcacaactcc
     2881 aaatactctg aatccaaatg acatcacaaa cggggaaatt tttctcctaa tggtattgga
     2941 acagctgtct tgttcattac cctcatcacg accatcatca atctttatcg tgggctcatc
     3001 tgctgttgga gagctcgaag ttttagtgag ctctgacccc tgttcatacc tcacagtcgt
     3061 ctccatggat cccggctagc tacgtaggcg cgccttaatt aagaagttcc tatacttctg
     3121 atagaatagg aacttcttgc cttaacccag aaattatcac tgttattctt tagaatggtg
     3181 caaagagaag ttcctatact ttctagagaa taggaacttc cagaattcga tatcaagctt
     3241 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3301 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3361 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3421 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3481 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3541 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3601 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3661 ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3721 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3781 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     3841 taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc
     3901 tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt
     3961 gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat
     4021 ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa
     4081 gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga
     4141 ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct
     4201 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact
     4261 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa
     4321 ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc
     4381 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     4441 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4501 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     4561 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     4621 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     4681 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4741 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4801 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4861 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4921 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     4981 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5041 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5101 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5161 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5221 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5281 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5341 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5401 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5461 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5521 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5581 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5641 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5701 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5761 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5821 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5881 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5941 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6001 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6061 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6121 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6181 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6241 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6301 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6361 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6421 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6481 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6541 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6601 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6661 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6721 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6781 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6841 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6901 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6961 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7021 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7081 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
