LOCUS       sequence-397097-        2982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-tagged NBR1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Kurusu R, Fujimoto Y, Morishita H, Noshiro D, Takada S, Yamano K, 
            Tanaka H, Arai R, Kageyama S, Funakoshi T, Komatsu-Hirota S, Taka H,
            Kazuno S, Miura Y, Koike M, Wakai T, Waguri S, Noda NN, Komatsu M
  TITLE     Integrated proteomics identifies p62-dependent selective autophagy 
            of the supramolecular vault complex.
  JOURNAL   Dev Cell. 2023 May 9:S1534-5807(23)00191-0. doi: 
            10.1016/j.devcel.2023.04.015.
  PUBMED    37192622
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2023 May 9:S1534-5807(23)00191-0. doi: 
            10.1016/j.devcel.2023.04.015."
COMMENT     Sequence Label: pMRX-IRES-puro-3xFLAG-NBR1
FEATURES             Location/Qualifiers
     source          1..2982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaagg aattcggatc tatggaacca caggttactc taaatgtgac ttttaaaaat
      121 gaaattcaaa gctttctggt ttctgatcca gaaaatacaa cttgggctga tatcgaagct
      181 atggtaaaag tttcatttga tctgaatact attcaaataa aatacctgga tgaggaaaat
      241 gaagaggtat ccatcaacag tcaaggagaa tatgaagaag cgcttaagat ggcagttaaa
      301 cagggaaacc aactgcagat gcaagtccac gaagggcacc atgtcgttga tgaagcccca
      361 cccccagttg taggagcaaa acgactagct gccagggcag ggaagaagcc acttgcacat
      421 tactcttcac tggtgagagt cttgggatca gacatgaaga ccccagagga tcctgcagtg
      481 cagtcgtttc cacttgttcc atgtgacaca gaccagcctc aagacaagcc cccagactgg
      541 ttcacaagct acctggagac gttcagagaa caagtggtta acgaaacggt tgagaagctt
      601 gaacagaaat tacatgaaaa gcttgtcctc cagaacccat ccttgggttc ttgtccctca
      661 gaagtctcaa tgcctacttc agaagaaaca ttgtttttgc cagaaaacca gttcagctgg
      721 catattgctt gcaacaactg ccaaagaagg attgttggtg tccgctacca gtgtagccta
      781 tgcccatcct acaatatctg tgaagattgt gaagcagggc catatggcca tgacactaac
      841 cacgtcctgc tgaagttgcg gagacctgtt gtgggctcct ctgaaccgtt ctgtcactca
      901 aagtactcta ctcctcgtct tcctgctgct ctggaacaag tcaggctcca gaaacaggtt
      961 gataagaact ttcttaaagc agaaaagcaa aggttgcgag ctgagaagaa acaacgtaaa
     1021 gcagaggtca aggaacttaa aaagcagctt aaactccata ggaaaattca cctgtggaat
     1081 tcaatccatg gactccagag ccccaagtct cctttaggcc gacctgagag cttgctccag
     1141 tctaataccc tgatgctccc tttgcagccc tgtacctccg ttatgccaat gctcagtgca
     1201 gcatttgtgg atgagaattt gcctgatggg actcaccttc agccaggaac caagtttatc
     1261 aaacactgga ggatgaaaaa tacaggaaat gtaaagtgga gtgcagacac aaagctcaag
     1321 ttcatgtggg gaaacctgac tttggcttcc acagaaaaga aggatgtttt ggttccctgc
     1381 ctcaaggccg gccatgtggg agttgtatct gtggagttca ttgccccagc cttggaggga
     1441 acgtatactt cccattggcg tctttctcac aaaggccagc aatttgggcc tcgggtctgg
     1501 tgcagtatca tagtagatcc tttcccctcc gaagagagcc ctgataacat tgaaaagggc
     1561 atgatcagct caagcaaaac tgatgatctc acctgccagc aagaggaaac ttttcttctg
     1621 gctaaagaag aaagacagct tggtgaagtg actgagcaga cagaagggac agcagcctgc
     1681 atcccacaga aggcaaaaaa tgttgccagt gagagggagc tctacatccc atctgtggat
     1741 cttctgactg cccaggacct gctgtccttt gagctgttgg atataaacat tgttcaagag
     1801 ttggagagag tgccccacaa cacccctgtg gatgtgactc cctgcatgtc tcctctgcca
     1861 catgacagtc ctttaataga gaagccaggc ttggggcaga tagaggaaga gaatgaaggg
     1921 gcaggattta aagcacttcc tgattctatg gtgtcagtaa agaggaaggc tgagaacatt
     1981 gcttctgtgg aggaagcaga agaagacctg agtgggaccc agtttgtgtg tgagacagta
     2041 atccgatccc ttaccttgga tgctgcccca gaccacaacc ctccttgcag acagaagtcc
     2101 ttgcagatga catttgcctt gcctgaagga ccacttggaa atgagaagga ggagattatc
     2161 catatcgctg aggaagaagc tgtcatggag gaggaggagg atgaggagga tgaggaggag
     2221 gaggatgagc tcaaagatga agttcaaagt cagtcctctg cttcctcaga ggattacatc
     2281 atcatcctgc ctgagtgctt tgataccagc cgccccctgg gggattctat gtacagctct
     2341 gcgctctcac agccaggcct ggagcgaggt gctgaaggca agcctggggt tgaggctggg
     2401 caggaaccag ctgaggctgg ggaaagactc cctggagggg agaaccagcc acaggagcac
     2461 agcataagtg acatcctcac gacctcacag actctggaaa cagtgcccct aatcccagag
     2521 gtagtggagc ttccaccgtc actgcccagg agctctcctt gtgtacatca tcatggttcc
     2581 ccaggagtgg atttaccagt taccatacca gaagtttctt cagtccctga tcagatcaga
     2641 ggagagccca gaggctcatc aggacttgta aacagcagac agaagagcta tgaccactca
     2701 aggcaccatc atgggagcag cattgctgga ggactggtga agggggcttt gtctgttgct
     2761 gcctctgcat acaaggccct gtttgctggg ccaccagtca ctgcacagcc aataatttct
     2821 gaagatcaga cagcagccct gatggcccat ctctttgaaa tgggattctg tgacaggcag
     2881 ctgaacctac ggctgctgaa gaaacacaat tacaatatcc tgcaggttgt gacagaactt
     2941 cttcagttaa acaacaacga ctggtacagc caacgctatt ga
//
