LOCUS       sequence-397128-        6898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Double floxed genetically encoded voltage indicator (GEVI) JEDI-1P 
            in AAV production vector expressed under the mammalian promoter 
            (EF1a).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Lu X, Wang Y, Liu Z, Gou Y, Jaeger D, St-Pierre F
  TITLE     Widefield imaging of rapid pan-cortical voltage dynamics with an 
            indicator evolved for one-photon microscopy.
  JOURNAL   Nat Commun. 2023 Oct 12;14(1):6423. doi: 10.1038/s41467-023-41975-3.
  PUBMED    37828037
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-12"; volume: "14(1):6423. doi"; pages: " 
            10.1038/s41467-023-41975-3"
COMMENT     Sequence Label: pAAV-EF1a-DIO-JEDI-1P-WPRE
FEATURES             Location/Qualifiers
     source          1..6898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   2..131
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        232..1410
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          463..1401
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1358..1378
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(1441..1474)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1525..1558)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2864..2873
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    2884..2917
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    2968..3001
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3026..3614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3079..3099)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3497..3508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3646..4122
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3845..3864)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4162..4302
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4162..4291
                     /label=AAV2 ITR
     rep_origin      4377..4832
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4464..4483)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4674..4695
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4849..4868)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4968..4990
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5028..5046)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5114..5218
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5219..5287,5288..6079)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5437..5456)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6250..6838
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6739..6758
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt ttaaacagag
      181 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
      241 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      301 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      361 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      421 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      481 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      541 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      601 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      661 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      721 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      781 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
      841 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
      901 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
      961 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     1021 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     1081 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     1141 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     1201 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     1261 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     1321 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     1381 agtttttttc ttccatttca ggtgtcgtga ggtaccggat cctctagagt cgactccgga
     1441 ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg attgtagctg
     1501 ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac gaagttatgg
     1561 cgcgccctat tacttgtaca tcattaggtt accacttcaa gttgtttctt ctgtgaagcc
     1621 aggcgaaata ttcttattaa gataacaatt ttcagaactc gaagaagagt aaccatgtgc
     1681 ggcatctgat ctgtgttata ttccagttta tgccccagaa tgttgccatc ttctttaaaa
     1741 tcggtgcctt tcagttcaat gcggttcacc agggtatcgc cttcaaattt caccaccgcg
     1801 cgggttttat atttgccatc atctttaaag ctaatggtgc gttcctgcac atagccttcc
     1861 ggcatcgcgc ttttaaaaaa atcatggcgt ttcatatgat ccggatagcg gctaaagcac
     1921 tgcacgccat aggttaaggt ggtcaccagg gtcggccacg gcaccggtag tttgccggtg
     1981 gtgcaaataa atttcagggt cagtttgcca atggtcgcat cgccttcgcc ttcgccgcgc
     2041 acgctaaatt tatggccgtt cacatcgcca tccagttcca ccagaatcgg caccacgccg
     2101 gtaaacagtt cttcgccctg gctcgcgctg ccgccggtgc cgccatacag ttcatccatg
     2161 ccgtgtgtaa tgcccgctgc ggtcacaaat tccagcagca ccatgtgatc gcgtttttcg
     2221 ttcggatctt tgctcagcac ggtctgggtg ctcagataat ggttatccgg cagcagcacc
     2281 gggccatcgc caatcggggt gttctgctga taatgatccg ccagctgcac gctgccatct
     2341 tccacgttat ggcgcacggt aaagttcgct ttaatgccgt ttttctgttt atccgcggta
     2401 atatacacgt tctcgctgtt aaaggcagca aggtcagaga aggagtaggt catattaatt
     2461 agcagagtgc ccactactat gactgcatcc aaagtaacca gtttggaccg gaaatagttc
     2521 ttgaagcctt caacaaacac tctcatgaga acatcaacaa ggaagaagag tgctatagcc
     2581 agggaaacac cttcaagaat ctctctaatg cctcttttct tctcactgat ggccagatcc
     2641 acaatcacca ctatgatgtc tacaatgata agcacaactc caaatactct gaatccaaat
     2701 gacatcacaa acggggaaat ttttctccta atggtattgg aacagctgtc ttgttcatta
     2761 ccctcatcac gaccatcatc aatctttatc gtgggctcat ctgctgttgg agagctcgaa
     2821 gttttagtga gctctgaccc ctgttcatac ctcacagtcg tctccatggt ggcggtagct
     2881 agcataactt cgtataaagt atcctatacg aagttatttg ccttaaccca gaaattatca
     2941 ctgttattct ttagaatggt gcaaagaata acttcgtata atgtatgcta tacgaagtta
     3001 tgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     3061 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3121 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3181 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3241 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3301 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3361 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3421 atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc
     3481 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3541 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3601 ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg gcatccctgt
     3661 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     3721 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     3781 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     3841 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     3901 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     3961 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     4021 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     4081 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc
     4141 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     4201 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4261 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
     4321 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     4381 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4441 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4501 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4561 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     4621 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4681 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
     4741 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4801 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     4861 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     4921 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     4981 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5041 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5101 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5161 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5221 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5281 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5341 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5401 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5461 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5521 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5581 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5641 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     5701 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     5761 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     5821 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     5881 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     5941 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6001 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6061 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6121 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6181 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6241 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6301 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6361 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6421 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6481 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6541 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6601 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6661 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6721 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6781 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6841 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatg
//
