LOCUS       sequence-397134-        7132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Double floxed soma and AIS-localized genetically encoded voltage 
            indicator (GEVI) JEDI-1P in AAV production vector expressed under 
            the mammalian promoter (EF1a).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Lu X, Wang Y, Liu Z, Gou Y, Jaeger D, St-Pierre F
  TITLE     Widefield imaging of rapid pan-cortical voltage dynamics with an 
            indicator evolved for one-photon microscopy.
  JOURNAL   Nat Commun. 2023 Oct 12;14(1):6423. doi: 10.1038/s41467-023-41975-3.
  PUBMED    37828037
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-10-12"; volume: "14(1):6423. doi"; pages: " 
            10.1038/s41467-023-41975-3"
COMMENT     Sequence Label: pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE
FEATURES             Location/Qualifiers
     source          1..7132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   2..131
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        232..1410
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          463..1401
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1358..1378
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(1441..1474)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1525..1558)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3098..3107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3118..3151
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3202..3235
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3260..3848
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3313..3333)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3731..3742)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3880..4356
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4079..4098)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4396..4536
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4396..4525
                     /label=AAV2 ITR
     rep_origin      4611..5066
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4698..4717)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4908..4929
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5083..5102)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5202..5224
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5262..5280)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5348..5452
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5453..5521,5522..6313)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5671..5690)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6484..7072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6973..6992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt ttaaacagag
      181 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
      241 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
      301 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
      361 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
      421 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
      481 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
      541 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
      601 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
      661 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
      721 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
      781 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
      841 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
      901 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
      961 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     1021 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     1081 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     1141 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     1201 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     1261 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     1321 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     1381 agtttttttc ttccatttca ggtgtcgtga ggtaccggat cctctagagt cgactccgga
     1441 ataacttcgt ataggatact ttatacgaag ttatgcagaa tggtagctgg attgtagctg
     1501 ctattagcaa tatgaaacct cttaataact tcgtatagca tacattatac gaagttatgg
     1561 cgcgccctat tacttgtaca ttatcaaaag cgggtcgctt ccggaaaatc ggtcgcgcag
     1621 ctaataaagc tatcaatgct gctcatgctg cgcatatcaa tcacgccttc ggtgcgcgcc
     1681 ggcagcggcg ccaccgggct cggcatgctg ctcatttcca gttcttccgg cggtttgctc
     1741 tgcggcgcca tttctttggt gttcagaatc ggctggctct gacgcgtgga agatccagat
     1801 gaacctgaag aacccttaag ggttaccact tcaagttgtt tcttctgtga agccaggcga
     1861 aatattctta ttaagataac aattttcaga actcgaagaa gagtaaccat gtgcggcatc
     1921 tgatctgtgt tatattccag tttatgcccc agaatgttgc catcttcttt aaaatcggtg
     1981 cctttcagtt caatgcggtt caccagggta tcgccttcaa atttcaccac cgcgcgggtt
     2041 ttatatttgc catcatcttt aaagctaatg gtgcgttcct gcacatagcc ttccggcatc
     2101 gcgcttttaa aaaaatcatg gcgtttcata tgatccggat agcggctaaa gcactgcacg
     2161 ccataggtta aggtggtcac cagggtcggc cacggcaccg gtagtttgcc ggtggtgcaa
     2221 ataaatttca gggtcagttt gccaatggtc gcatcgcctt cgccttcgcc gcgcacgcta
     2281 aatttatggc cgttcacatc gccatccagt tccaccagaa tcggcaccac gccggtaaac
     2341 agttcttcgc cctggctcgc gctgccgccg gtgccgccat acagttcatc catgccgtgt
     2401 gtaatgcccg ctgcggtcac aaattccagc agcaccatgt gatcgcgttt ttcgttcgga
     2461 tctttgctca gcacggtctg ggtgctcaga taatggttat ccggcagcag caccgggcca
     2521 tcgccaatcg gggtgttctg ctgataatga tccgccagct gcacgctgcc atcttccacg
     2581 ttatggcgca cggtaaagtt cgctttaatg ccgtttttct gtttatccgc ggtaatatac
     2641 acgttctcgc tgttaaaggc agcaaggtca gagaaggagt aggtcatatt aattagcaga
     2701 gtgcccacta ctatgactgc atccaaagta accagtttgg accggaaata gttcttgaag
     2761 ccttcaacaa acactctcat gagaacatca acaaggaaga agagtgctat agccagggaa
     2821 acaccttcaa gaatctctct aatgcctctt ttcttctcac tgatggccag atccacaatc
     2881 accactatga tgtctacaat gataagcaca actccaaata ctctgaatcc aaatgacatc
     2941 acaaacgggg aaatttttct cctaatggta ttggaacagc tgtcttgttc attaccctca
     3001 tcacgaccat catcaatctt tatcgtgggc tcatctgctg ttggagagct cgaagtttta
     3061 gtgagctctg acccctgttc atacctcaca gtcgtctcca tggtggcggt agctagcata
     3121 acttcgtata aagtatccta tacgaagtta tttgccttaa cccagaaatt atcactgtta
     3181 ttctttagaa tggtgcaaag aataacttcg tataatgtat gctatacgaa gttatgaatt
     3241 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3301 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3361 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3421 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3481 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3541 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3601 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3661 gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg
     3721 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3781 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3841 ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc
     3901 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     3961 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     4021 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     4081 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     4141 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     4201 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     4261 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     4321 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc
     4381 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     4441 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     4501 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     4561 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     4621 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4681 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4741 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4801 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     4861 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4921 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     4981 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5041 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     5101 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     5161 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     5221 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     5281 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     5341 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5401 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5461 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5521 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5581 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5641 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5701 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5761 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5821 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5881 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5941 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6001 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6061 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     6121 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     6181 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     6241 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     6301 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6361 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6421 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6481 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6541 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6601 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6661 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6721 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6781 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6841 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6901 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6961 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7021 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7081 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tg
//
