LOCUS       sequence-397155-       11815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2443
FEATURES             Location/Qualifiers
     source          1..11815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7672..8260
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7725..7745)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8143..8154)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8332..8565
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8643..8764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8680..8699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8734..8753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8804..8939
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8959..8978)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8960..8978)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8988..9005)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8988..9004)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8997..9019)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9146..9601
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9233..9252)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9443..9464
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9627..9731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9732..9800,9801..10592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9950..9969)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10763..11351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11252..11271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11505..11522
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11639..11660
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11675..11680,11681..11698,11699..11705)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11713..11729
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11718..11740
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11737..11753
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11737..11753
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11772..11792
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11774..11792
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgggg cagcagcctg gaaaagtact
     4141 tggggaccaa agaaggccaa gcttgcctgc cctgcatttt atcaaaggag cagggaagaa
     4201 ggaatcatcg aggcatgggg gtccacactg caatgttttt gtggaacatg aagcccttca
     4261 gcggccagta gcatctgact ttgagcctca gggtctgagt gaagccgctc gttggaactc
     4321 caaggaaaac cttctcgctg gacccagtga aaatgacccc aaccttttcg ttgcactgta
     4381 tgattttgtg gccagtggag ataacactct aagcataact aaaggtgaaa agctccgggt
     4441 cttaggctat aatcacaatg gggaatggtg tgaagcccaa accaaaaatg gccaaggctg
     4501 ggtcccaagc aactacatca cgccagtcaa cagtctggag aaacactcct ggtaccatgg
     4561 gcctgtgtcc cgcaatgccg ctgagtatct gctgagcagc gggatcaatg gcagcttctt
     4621 ggtgcgtgag agtgagagca gtcctggcca gaggtccatc tcgctgagat acgaagggag
     4681 ggtgtaccat tacaggatca acactgcttc tgatggcaag ctctacgtct cctccgagag
     4741 ccgcttcaac accctggccg agttggttca tcatcattca acggtggccg acgggctcat
     4801 caccacgctc cattatccag ccccaaagcg caacaagccc actgtctatg gtgtgtcccc
     4861 caactacgac aagtgggaga tggaacgcac ggacatcacc atgaagcaca agctgggcgg
     4921 gggccagtac ggggaggtgt acgagggcgt gtggaagaaa tacagcctga cggtggccgt
     4981 gaagaccttg aaggaggaca ccatggaggt ggaagagttc ttgaaagaag ctgcagtcat
     5041 gaaagagatc aaacacccta acctggtgca gctccttggg gtctgcaccc gggagccccc
     5101 gttctatatc atcactgagt tcatgaccta cgggaacctc ctggactacc tgagggagtg
     5161 caaccggcag gaggtgaacg ccgtggtgct gctgtacatg gccactcaga tctcgtcagc
     5221 catggagtac ctggagaaga aaaacttcat ccacagagat cttgctgccc gaaactgcct
     5281 ggtaggggag aaccacttgg tgaaggtagc tgattttggc ctgagcaggt tgatgacagg
     5341 ggacacctac acagcccatg ctggagccaa gttccccatc aaatggactg cacccgagag
     5401 cctggcctac aacaagttct ccatcaagtc cgacgtctgg gcatttggag tattgctttg
     5461 ggaaattgct acctatggca tgtcccctta cccgggaatt gacctgtccc aggtgtatga
     5521 gctgctagag aaggactacc gcatggagcg cccagaaggc tgcccagaga aggtctatga
     5581 actcatgcga gcatgttggc agtggaatcc ctctgaccgg ccctcctttg ctgaaatcca
     5641 ccaagccttt gaaacaatgt tccaggaatc cagtatctca gacgaagtgg aaaaggagct
     5701 ggggaaacaa ggcgtccgtg gggctgtgag taccttgctg caggccccag agctgcccac
     5761 caagacgagg acctccagga gagctgcaga gcacagagac accactgacg tgcctgagat
     5821 gcctcactcc aagggccagg gagagagcga tcctctggac catgagcctg ccgtgtctcc
     5881 attgctccct cgaaaagagc gaggtccccc ggagggcggc ctgaatgaag atgagcgcct
     5941 tctccccaaa gacaaaaaga ccaacttgtt cagcgccttg atcaagaaga agaagaagac
     6001 agccccaacc cctcccaaac gcagcagctc cttccgggag atggacggcc agccggagcg
     6061 cagaggggcc ggcgaggaag agggccgaga catcagcaac ggggcactgg ctttcacccc
     6121 cttggacaca gctgacccag ccaagtcccc aaagcccagc aatggggctg gggtccccaa
     6181 tggagccctc cgggagtccg ggggctcagg cttccggtct ccccacctgt ggaagaagtc
     6241 cagcacgctg accagcagcc gcctagccac cggcgaggag gagggcggtg gcagctccag
     6301 caagcgcttc ctgcgctctt gctccgcctc ctgcgttccc catggggcca aggacacgga
     6361 gtggaggtca gtcacgctgc ctcgggactt gcagtccacg ggaagacagt ttgactcgtc
     6421 cacatttgga gggcacaaaa gtgagaagcc ggctctgcct cggaagaggg caggggagaa
     6481 caggtctgac caggtgaccc gaggcacagt aacgcctccc cccaggctgg tgaaaaagaa
     6541 tgaggaagct gctgatgagg tcttcaaaga catcatggag tccagcccgg gctccagccc
     6601 gcccaacctg actccaaaac ccctccggcg gcaggtcacc gtggcccctg cctcgggcct
     6661 cccccacaag gaagaagctg gaaagggcag tgccttaggg acccctgctg cagctgagcc
     6721 agtgaccccc accagcaaag caggctcagg tgcaccaggg ggcaccagca agggccccgc
     6781 cgaggagtcc agagtgagga ggcacaagca ctcctctgag tcgccaggga gggacaaggg
     6841 gaaattgtcc aggctcaaac ctgccccgcc gcccccacca gcagcctctg cagggaaggc
     6901 tggaggaaag ccctcgcaga gcccgagcca ggaggcggcc ggggaggcag tcctgggcgc
     6961 aaagacaaaa gccacgagtc tggttgatgc tgtgaacagt gacgctgcca agcccagcca
     7021 gccgggagag ggcctcaaaa agcccgtgct cccggccact ccaaagccac agtccgccaa
     7081 gccgtcgggg acccccatca gcccagcccc cgttccctcc acgttgccat cagcatcctc
     7141 ggccctggca ggggaccagc cgtcttccac cgccttcatc cctctcatat caacccgagt
     7201 gtctcttcgg aaaacccgcc agcctccaga gcggatcgcc agcggcgcca tcaccaaggg
     7261 cgtggtcctg gacagcaccg aggcgctgtg cctcgccatc tctaggaact ccgagcagat
     7321 ggccagccac agcgcagtgc tggaggccgg caaaaacctc tacacgttct gcgtgagcta
     7381 tgtggattcc atccagcaaa tgaggaacaa gtttgccttc cgagaggcca tcaacaaact
     7441 ggagaataat ctccgggagc ttcagatctg cccggcgaca gcaggcagtg gtccagcggc
     7501 cactcaggac ttcagcaagc tcctcagttc ggtgaaggaa atcagtgaca tagtgcagag
     7561 gtagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     7621 tggaaaggac gatcggatat gtctttgtgc catctaacgc gttaagtcga caatcaacct
     7681 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7741 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7801 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7861 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7921 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7981 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8041 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     8101 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8161 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     8221 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     8281 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8341 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8401 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8461 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8521 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     8581 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     8641 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8701 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8761 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     8821 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8881 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8941 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     9001 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     9061 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     9121 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9181 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     9241 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9301 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9361 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9421 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9481 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     9541 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     9601 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9661 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9721 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9781 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9841 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9901 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9961 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10021 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10081 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    10141 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    10201 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10261 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10321 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10381 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10441 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10501 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10561 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10621 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10681 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10741 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10801 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10861 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10921 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10981 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11041 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    11101 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    11161 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11221 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11281 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11341 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11401 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    11461 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    11521 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    11581 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    11641 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    11701 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    11761 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
