LOCUS       sequence-397168-       12169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0224
FEATURES             Location/Qualifiers
     source          1..12169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8026..8614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8079..8099)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8497..8508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8686..8919
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8997..9118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9034..9053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9088..9107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9158..9293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9313..9332)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9314..9332)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9342..9359)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9342..9358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9351..9373)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9500..9955
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9587..9606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9797..9818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9981..10085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10086..10154,10155..10946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10304..10323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11117..11705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11606..11625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11859..11876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11993..12014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12029..12034,12035..12052,12053..12059)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12067..12083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12072..12094
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12091..12107
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12091..12107
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12126..12146
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12128..12146
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgggg gatatgaaaa ccccagattt
     4141 tgatgacctt ctggctgcct ttgacatccc agaccccacc agccttgatg ccaaggaggc
     4201 catccagaca cccagtgagg agaatgagag tcccctcaaa cctccaggca tatgtatgga
     4261 tgaaagtgtg tccttgtctc actcaggatc agcccccgat gtgccggccg tgagtgtcat
     4321 tgtcaagaac accagccgcc aggagtcatt tgaagcggag aaagaccaca ttactcccag
     4381 tctcctacac aatggattcc ggggctcaga tctgcctcca gatccccaca actgtgggaa
     4441 atttgattct acttttatga atggagacag tgccaggagt ttccctggca aactggagcc
     4501 tcccaagtca gagccattac ccaccttcaa ccagttcagt ccaatctcca gcccagaacc
     4561 tgaggatccc atcaaagata acggatttgg gataaagccc aaacactctg acagttattt
     4621 cccaccccct cttgggtgcg gggctgtggg aggcccagtc ctggaggctc tggctaagtt
     4681 tccggttcca gagctgcata tgtttgatca tttttgtaag aaagaaccca agccagaacc
     4741 cctgcccttg gggagccagc aggaacacga gcaaagtggg cagaacacag tggaacctca
     4801 caaggatccg gatgccactc gattcttcgg ggaagctttg gagttcaaca gccatcctag
     4861 caacagtatt ggagagtcca aggggcttgc ccgggagctt ggtacctgct catcagtccc
     4921 ccctaggcag cgtctaaagc cagctcattc caagctgtcc tcttgtgtgg cagccttggt
     4981 ggccttgcag gccaaaagag tggctagtgt cactaaggag gatcagcctg gccacacaaa
     5041 ggatctctca gggcccacta aagagagttc taaaggtagc cccaaaatgc ccaagtcacc
     5101 aaagagtccc cggagccctc tggaggccac tagaaaaagt atcaagccat cggacagccc
     5161 tcgtagcatc tgcagtgaca gcagcagcaa aggctcaccg tctgtggctg ccagctcccc
     5221 accagcaatt cccaaagtga gaatcaaaac cattaagaca tcatcagggg aaatcaaacg
     5281 gactgtcaca aggatcctgc cagatcctga tgatccaagt aagtcccctg ttgggtcacc
     5341 tctagggagc gccattgcag aggcccccag cgagatgcca ggggatgagg tgcctgtgga
     5401 agagcacttt cctgaggcag gcacaaattc agggagcccc cagggggcca ggaaagggga
     5461 cgagagcatg acaaaggcca gtgactcgtc atctcccagc tgcagttctg ggccccgggt
     5521 cccaaagggg gctgccccag gctcacagac aggcaagaag caacagagca cagcactgca
     5581 ggcatccacc ctggcccctg ccaacctcct gcccaaagcc gtgcacttgg ccaacctgaa
     5641 cctcgtcccc cacagtgttg ctgcatcagt gacagccaag tcttcagtgc aaagacggag
     5701 ccagccacag cttacacaaa tgtcggtgcc cctggtccac caggtgaaaa aggctgcccc
     5761 actgattgta gaggtcttca acaaggtcct tcacagctcc aaccccgtgc ccctctatgc
     5821 gccaaatctc agcccgcctg cggacagcag gatccacgtg ccggccagtg ggtactgctg
     5881 cctggagtgt ggagacgcat ttgccttaga gaagagcctg agccagcact atggccggcg
     5941 gagcgtccac attgaggtac tgtgcacact gtgctccaag acgctgctct tcttcaacaa
     6001 gtgcagcctg ctccggcacg cccgtgacca caagagcaag gggctcgtca tgcagtgttc
     6061 ccagctgctg gtgaagccta tctctgcgga ccaaatgttc gtgtcggccc ctgtgaactc
     6121 cacggcacca gcagccccag ccccttcatc ctctcccaaa catggcctca cttcgggcag
     6181 tgccagtccc cctcctccag ccttgccact ctacccagac cctgtgaggc tcatccggta
     6241 ctcaatcaag tgtcttgaat gtcacaagca gatgcgggac tacatggtcc tggctgcaca
     6301 tttccagagg acaacagagg agacagaggg gctgacctgc caggtatgcc aaatgctgct
     6361 gcccaaccag tgcagtttct gtgcccacca gcggattcat gcacacaagt ccccctactg
     6421 ctgcccggag tgtggggtcc tctgccgctc tgcctacttc cagacccatg taaaggagaa
     6481 ttgcctgcac tatgcccgca aggtgggcta caggtgcatc cactgtggtg tcgtccacct
     6541 gaccttggcc ttgctgaaaa gccacatcca ggagcgacac tgccaggttt tccacaaatg
     6601 tgcattctgc cccatggcct tcaagactgc cagcagcact gcagaccaca gtgccaccca
     6661 gcaccccacc cagccccaca gaccctccca gctcatttat aagtgctcct gtgaaatggt
     6721 cttcaacaag aagaggcaca ttcagcagca tttttaccag aatgtcagca agacgcaggt
     6781 gggcgtcttc aagtgccctg agtgcccact cttgttcgtg cagaagccgg agttgatgca
     6841 acacgtcaag agcacccacg gtgttccccg aaatgtggac gagctgtcaa gcctccagtc
     6901 ttcagcggac acatcctcaa gccgccctgg ctctcgagtt cccactgagc caccagccac
     6961 tagtgtggct gctcggagca gctccctgcc ttctggccgc tggggtaggc ctgaagccca
     7021 ccgcagggtg gaagccaggc cgcggctgag gaacactggc tggacctgcc aggagtgcca
     7081 ggagtgggtt ccagatcggg agagctacgt gtcccacatg aaaaagagcc acggtcggac
     7141 attgaagcgg tacccatgcc ggcagtgtga acagtccttc cacaccccca acagcctgcg
     7201 caaacacatc cgcaacaacc atgacacagt aaagaagttc tacacctgcg ggtactgcac
     7261 agaggacagc cccagctttc ctcggccctc ccttctggag agccacatca gccttatgca
     7321 tggcatcaga aaccctgatt tgagccagac gtccaaagtg aaacctccgg gtggacattc
     7381 ccctcaggtg aaccatctga aaagaccagt cagtggagtg ggggacgctc caggcaccag
     7441 caatggcgca actgtctctt ccaccaaaag gcacaagtcc ctttttcagt gcgcgaaatg
     7501 tagttttgcc acagactcgg ggctcgagtt tcagagccac atacctcagc accaggtgga
     7561 cagctccaca gcccaatgtc tcctctgtgg tttgtgctac acctctgcca gctccctcag
     7621 ccgccacctc ttcattgtcc acaaggtgag agaccaggag gaggaggagg aagaggaggc
     7681 ggcggcagcg gagatggcag tggaggtggc agagccagag gagggctccg gggaggaggt
     7741 gcccatggag actagagaga atggactgga agaatgtgcc ggtgagcctt tgtcagctga
     7801 cccagaggcg aggagattgc tgggcccggc ccctgaggac gatggtggcc acaatgatca
     7861 cagtcaacca caggcctctc aggaccagga cagccacaca ctgtcccctc aggtgtagta
     7921 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     7981 ggacgacccg ggtccagtga gagcttgcag acgcgttaag tcgacaatca acctctggat
     8041 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8101 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8161 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8221 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8281 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8341 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8401 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8461 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8521 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8581 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     8641 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8701 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8761 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8821 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8881 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     8941 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     9001 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9061 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9121 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9181 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9241 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     9301 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     9361 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9421 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9481 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9541 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9601 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9661 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9721 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9781 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9841 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9901 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     9961 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10021 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10081 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10141 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10201 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    10261 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    10321 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    10381 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10441 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10501 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10561 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    10621 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10681 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10741 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10801 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10861 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10921 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10981 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11041 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    11281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11761 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11821 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11881 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11941 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    12001 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    12061 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    12121 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
