LOCUS       sequence-397179-       12106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1307
FEATURES             Location/Qualifiers
     source          1..12106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     RBS             5371..5379
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    7963..8551
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8016..8036)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8434..8445)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8623..8856
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8934..9055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8971..8990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9025..9044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9095..9230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9250..9269)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9251..9269)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9279..9296)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9279..9295)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9288..9310)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9437..9892
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9524..9543)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9734..9755
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9918..10022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10023..10091,10092..10883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10241..10260)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11054..11642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11543..11562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11796..11813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11930..11951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11966..11971,11972..11989,11990..11996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12004..12020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12009..12031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12028..12044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12028..12044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12063..12083
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12065..12083
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgagc aaggaaagac ccaagaggaa
     4141 tatcattcag aagaaatacg atgacagtga tgggattccg tggtcagaag aacgggtggt
     4201 acgtaaagtc ctttatttgt ctctgaagga gttcaagaat tcccagaaga ggcagcatgc
     4261 ggaaggcatt gctgggagcc tgaaaactgt gaatgggctc cttggtaatg accagtctaa
     4321 gggattagga ccagcatcag aacagtcaga gaatgaaaag gacgatgcat cccaagtgtc
     4381 ctccactagc aacgatgtta gttcttcaga ttttgaagaa gggccgtcga ggaaaaggcc
     4441 caggctgcaa gcacaaagga agtttgctca gtctcagccg aatagtccca gcacaactcc
     4501 agtaaagata gtggagccat tgctaccccc tccagctact cagatatcag acctctctaa
     4561 aaggaagcct aagacagaag attttcttac ctttctctgc cttcgaggtt ctcctgcgct
     4621 gcccaacagc atggtgtatt ttggaagctc tcaggatgag gaggaagtcg aggaggaaga
     4681 tgatgagaca gaagacgtca aaacagccac caacaatgct tcatcttcat gccagtcgac
     4741 ccccaggaaa ggaaaaaccc acaaacatgt tcacaacggg catgttttca atggttccag
     4801 caggtcaaca cgggagaagg aacctgttca aaaacacaaa agcaaagagg ccactcccgc
     4861 aaaggagaag cacagcgatc accgggctga cagccgccgg gagcaggctt cagctaacca
     4921 ccccgcagcg gccccctcca cgggttcctc ggccaagggg cttgctgcca cccatcacca
     4981 cccccctctg catcggtcgg ctcaggactt acggaaacag gtttctaagg taaacggagt
     5041 cactcgaatg tcatctctgg gtgcaggtgt aaccagtgcc aaaaagatgc gcgaggtcag
     5101 accttcacca tccaaaactg tgaagtacac tgccacggtg acgaaggggg ctgtcacata
     5161 caccaaagcc aagagagaac tggtcaagga caccaaaccc aatcaccaca agcccagttc
     5221 cgctgtcaac cacacaatct cagggaaaac tgaaagtagc aatgcaaaaa cccgcaaaca
     5281 ggtgctatcc ctcggggggg cgtccaagtc cactgggccc gccgtcaatg gcctcaaggt
     5341 cagtggcagg ttgaacccaa agtcatgcac taaggaggtg ggggggcggc agctgcggga
     5401 gggcctgcag ctgcgggagg ggctgcggaa ctccaagagg agactggaag aggcacacca
     5461 ggcggagaag ccgcagtcgc cccccaagaa gatgaaaggg gcggctggcc ccgccgaagg
     5521 ccctggcaag aaggccccgg ccgagagagg tctgctgaac ggacacgtga agaaggaagt
     5581 gccggagcgc agtctggaga ggaatcggcc gaagcgggcc acggccggga agagcacgcc
     5641 aggcagacaa gcacatggca aggcggacag cgcctcctgt gaaaatcgtt ctacctcgca
     5701 accggagtcc gtgcacaagc cgcaggactc gggcaaggcc gagaagggcg gcggcaaggc
     5761 cgggtgggcg gccatggacg agatccccgt cctcaggccc tccgccaagg agttccacga
     5821 tccgctcatc tacatcgagt cggtccgcgc tcaggtggag aagttcggga tgtgcagggt
     5881 gatcccccct ccggactggc ggcccgagtg caagctcaac gatgagatgc ggtttgtcac
     5941 gcagattcag cacatccaca agctgggccg gcgctggggc cccaacgtgc agcggctggc
     6001 ctgcatcaag aagcacctca aatctcaggg catcaccatg gacgagctcc cgctcatagg
     6061 gggctgtgag ctcgacctgg cctgcttttt ccggctgatt aatgagatgg gcggcatgca
     6121 gcaagtgact gacctcaaaa aatggaacaa actagcagac atgctgcgca tccccagaac
     6181 tgcccaggac cggctggcca agctgcagga ggcctactgc cagtacctac tctcctacga
     6241 ctccctgtcc ccagaggagc accggcggct ggagaaggag gtgctgatgg agaaggagat
     6301 cctggagaag cgcaaggggc cgctggaagg ccacacagag aacgaccacc acaagttcca
     6361 ccctctgccc cgcttcgagc ccaagaatgg gctcatccac ggcgtggccc ccaggaacgg
     6421 cttccgcagc aagctcaagg aggtgggcca ggcccagttg aagactggcc ggcggcgact
     6481 cttcgctcag gaaaaagaag tggtcaagga agaggaggag gacaaaggcg tcctcaatga
     6541 cttccacaag tgcatctata agggaaggtc tgtttctcta acaacttttt atcgaacagc
     6601 gaggaatatc atgagcatgt gtttcagcaa ggagcctgcc ccagccgaaa tcgagcaaga
     6661 gtactggagg ctagtggaag agaaggactg ccacgtggca gtgcactgcg gcaaggtgga
     6721 caccaacact cacggcagtg gattcccagt aggaaaatca gaaccctttt cgaggcatgg
     6781 atggaacctc accgtcctcc ccaataacac agggtccatc ctgcgtcacc tcggtgctgt
     6841 gcctggagtg actattccct ggctaaatat tggcatggtc ttttctacct catgctggtc
     6901 tcgagaccaa aatcaccttc catacattga ctacttacac actggtgctg actgcatttg
     6961 gtattgcatt cctgctgagg aggagaacaa gctggaagat gtggtccaca ccctgctgca
     7021 agccaatggc accccagggc tgcagatgct ggaaagcaac gtcatgatct ccccggaggt
     7081 gctgtgcaaa gaggggatca aggtgcacag gaccgtgcag cagagtggcc agtttgtcgt
     7141 ctgcttcccg ggatcctttg tgtccaaagt gtgctgtggg tacagcgtgt ctgaaaccgt
     7201 gcactttgct accacccagt ggacaagtat gggctttgag accgccaagg aaatgaagcg
     7261 tcgccatata gctaagccat tctccatgga gaagttactc taccagattg cacaagcaga
     7321 agcaaaaaaa gaaaacggtc ccactctcag taccatctca gccctcctgg atgagctcag
     7381 ggatacagag ctgcggcagc gcaggcagct gttcgaggct ggcctccact cctccgcacg
     7441 ctatggcagc cacgatggca gcagcacggt ggcggacggg aagaaaaagc ctcgaaagtg
     7501 gctgcagttg gagacgtcag agaggaggtg tcagatctgc cagcacctgt gctacctgtc
     7561 catggtggta caagagaacg aaaacgtcgt gttctgtctg gagtgtgctc tgcgccacgt
     7621 ggagaaacag aagtcctgcc gagggctgaa gttgatgtac cgctacgatg aggaacagat
     7681 tatcagtctg gtcaatcaga tctgcggcaa agtgtctggt aaaaacggca gcattgagaa
     7741 ctgtctcagt aaacccacac caaaaagagg tccccgcaag agagcgacag tggacgtgcc
     7801 cccctcccgt ctgtcagcct ccagttcatc caaaagtgct tcgagctcat catagtaatg
     7861 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     7921 cgagtcgcta ccctcgtaac ggcttgtacg cgttaagtcg acaatcaacc tctggattac
     7981 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     8041 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     8101 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     8161 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8221 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8281 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8341 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8401 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8461 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8521 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     8581 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     8641 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     8701 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8761 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8821 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     8881 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     8941 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9001 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9061 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9121 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     9241 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     9301 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     9361 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     9421 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9481 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9541 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9601 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9661 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     9721 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     9781 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     9841 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     9901 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9961 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    10021 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    10081 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
    10141 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
    10201 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
    10261 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
    10321 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
    10381 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10441 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10501 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10561 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10621 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10681 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10741 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10801 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10861 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10921 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10981 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    11041 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    11101 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    11161 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    11221 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    11281 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    11341 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    11401 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    11461 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    11521 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11581 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11641 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11701 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11761 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    11821 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    11881 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    11941 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    12001 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    12061 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagc
//
