LOCUS       sequence-397180-       11869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1645
FEATURES             Location/Qualifiers
     source          1..11869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7726..8314
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7779..7799)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8197..8208)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8386..8619
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8697..8818
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8734..8753)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8788..8807
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8858..8993
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9013..9032)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9014..9032)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9042..9059)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9042..9058)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9051..9073)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9200..9655
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9287..9306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9497..9518
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9681..9785
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9786..9854,9855..10646)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10004..10023)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10817..11405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11306..11325
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11559..11576
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11693..11714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11729..11734,11735..11752,11753..11759)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11767..11783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11772..11794
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11791..11807
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11791..11807
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11826..11846
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11828..11846
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgatc ggagatggtt gtgacctcgg
     4141 cctcggcgag gaggaagggg gcacgggcct gccataccct tgccagttct gcgacaagtc
     4201 cttcatccgc ttgagctact tgaagaggca cgagcagatc cacagcgaca agctgccgtt
     4261 caagtgcacc tactgcagcc gcctcttcaa gcacaagagg agccgtgacc ggcacatcaa
     4321 gctgcatacg ggcgacaaga agtatcactg ccacgagtgc gaggcagcct tctcccgcag
     4381 cgaccacctc aagatccacc tgaagaccca cagctccagc aagcccttca agtgcactgt
     4441 gtgcaagcgc ggcttctcct ccaccagctc gctgcagagc cacatgcagg cccacaaaaa
     4501 gaacaaggag catctggcca agtcggagaa ggaagccaag aaggacgact tcatgtgcga
     4561 ctactgcgag gacaccttca gccagacgga ggagctggag aagcacgtgc tcacccgcca
     4621 cccgcagctg tccgagaagg cggacctgca gtgcattcac tgccctgagg tcttcgtcga
     4681 cgagaacaca ctgctcgccc atatccacca agcccacgcc aaccagaaac acaagtgccc
     4741 catgtgccct gagcagttct cctcagtgga aggtgtctac tgccacctgg acagccaccg
     4801 gcagcccgac tccagcaacc acagtgtcag tcccgaccct gtactgggca gcgtggcctc
     4861 catgagcagc gccacacccg actccagcgc ctctgtggag cgtggctcca ccccggactc
     4921 caccttgaag ccgctgcggg ggcagaagaa gatgcgggat gacgggcagg gctggaccaa
     4981 ggtggtctat agctgcccct attgttccaa gcgggacttt aacagcctgg ccgtgctgga
     5041 gatccacctg aagaccatcc acgcggacaa gccccagcag agccacacat gtcagatctg
     5101 cctggactcc atgcccaccc tctacaacct caacgagcac gttcgcaagc tgcacaagaa
     5161 ccatgcctac cctgtgatgc agtttggcaa catctctgcc ttccactgca actactgccc
     5221 cgagatgttc gccgacatca atagcctgca ggagcacatc cgcgtctccc actgcggccc
     5281 caacgccaac ccctctgacg gtaataatgc tttcttctgc aaccagtgct ccatgggttt
     5341 ccttactgag tcctccctca ccgagcacat ccagcaggcc cactgcagtg tgggcagtgc
     5401 caaactagag tctccggtgg tgcagcccac gcagtccttc atggaggtct attcctgccc
     5461 ctactgcacc aactccccca tctttggctc catcctgaaa ctcaccaagc acatcaagga
     5521 gaaccacaag aacattccac tggcccacag caagaagtcc aaggccgagc agagcccagt
     5581 ctcgtccgat gtggaggtgt cttccccgaa gcggcagcgg ctctcagcaa gcgccaactc
     5641 catctccaat ggggagtatc cttgcaatca atgcgacctc aagttctcca actttgagag
     5701 cttccagacc cacctgaagc tgcacctgga gctgctgctg cggaagcaag cgtgccccca
     5761 gtgcaaagag gactttgact cccaggagtc cctcctgcag cacctgacag tgcattacat
     5821 gaccacgtcg acccactatg tgtgcgagag ctgcgacaag caattttcct cggtggatga
     5881 cctgcagaag cacctgctgg acatgcacac ctttgtgttg taccactgca ccctgtgtca
     5941 ggaggtcttc gactccaagg tgtccatcca ggtgcacctg gcggtgaagc acagcaatga
     6001 gaagaagatg taccgctgca cggcctgcaa ctgggacttc cgcaaggagg ctgacctgca
     6061 ggtgcacgtc aaacacagcc acctgggcaa cccggccaag gctcacaagt gcatcttctg
     6121 tggggagacc ttcagcaccg aggtggagct gcagtgccac atcaccacac acagcaagaa
     6181 gtataactgt aagttctgca gcaaggcctt ccacgccatc atcctgctgg agaagcacct
     6241 gcgggagaag cactgtgtgt ttgatgctgc gaccgagaac ggcacggcca atggggtacc
     6301 cccaatggcc accaagaaag ctgagcctgc tgacctgcag ggcatgctgc ttaagaaccc
     6361 tgaggcacct aacagccatg aggccagcga ggatgacgtg gacgcgtcgg agcccatgta
     6421 cggctgtgac atctgtgggg cggcctacac catggaggtg ctgctgcaga atcaccggct
     6481 gcgggaccac aatatccggc cgggcgagga tgatggctca cgcaagaagg ctgagtttat
     6541 caagggcagt cacaagtgca acgtttgttc acggactttc ttctcggaga acgggctacg
     6601 ggagcacctg cagacgcacc ggggccctgc caagcactac atgtgtccca tctgtggtga
     6661 gcgcttccct tcgctgctga cgctcaccga acacaaggtg acccacagca agagcctgga
     6721 cacgggcacc tgtcgcatct gcaagatgcc cctgcagagc gaggaggagt ttattgagca
     6781 ctgccagatg caccctgacc tgcgcaactc actcacgggc ttccgctgtg tggtctgcat
     6841 gcagacagtc acttccacgc ttgagctcaa gatccatggc accttccaca tgcagaagct
     6901 ggcgggcagc tcagcggcgt cctcccccaa tggccagggg ctgcagaagc tctacaagtg
     6961 cgccctgtgc ctcaaggagt tccgcagcaa gcaggacctg gtgaagcttg acgtcaatgg
     7021 gctgccctac ggcctctgcg ccggctgcat ggcccgcagc gccaacggac aggtgggtgg
     7081 cctggccccg cccgagcccg ccgaccggcc ctgtgccggc ctccgttgcc ccgagtgcag
     7141 tgtcaagttt gagagtgccg aagacctgga gagccacatg caggtggacc accgtgacct
     7201 cacgccggag accagtgggc cccggaaagg cacccagaca tcgccagtgc cccggaaaaa
     7261 gacataccag tgcatcaagt gccagatgac cttcgagaac gagagagaga tccaaatcca
     7321 cgttgccaac cacatgattg aggaaggcat caaccacgag tgtaagctgt gcaaccagat
     7381 gttcgactcc ccggccaagc tcctctgtca cctcattgag cacagcttcg agggcatggg
     7441 cggcaccttc aaatgccccg tgtgtttcac agtcttcgtc caggccaaca agttgcagca
     7501 gcacatcttt gccgtgcacg ggcaggagga caagatctac gactgctcac agtgccctca
     7561 gaagttcttc ttccagaccg agctgcagaa ccacacgatg agccagcacg cacagtagta
     7621 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     7681 ggacgatgtc gtgtggaccg accagcttgg acgcgttaag tcgacaatca acctctggat
     7741 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     7801 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     7861 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7921 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7981 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8041 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8101 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     8161 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8221 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     8281 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     8341 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     8401 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8461 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8521 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8581 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     8641 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     8701 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8761 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8821 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8881 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8941 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     9001 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     9061 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9121 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9181 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9241 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     9301 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9361 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9421 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9481 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9541 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9601 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     9661 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     9721 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     9781 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9841 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9901 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9961 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    10021 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    10081 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    10141 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    10201 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    10261 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    10321 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    10381 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    10441 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    10501 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    10561 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    10621 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    10681 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10741 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10801 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10861 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10921 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10981 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    11041 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    11101 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    11161 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    11221 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    11281 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    11341 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    11401 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    11461 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    11521 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    11581 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    11641 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    11701 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    11761 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    11821 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
