LOCUS       sequence-397204-       12352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12352)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2282
FEATURES             Location/Qualifiers
     source          1..12352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8209..8797
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8262..8282)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8680..8691)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8869..9102
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9180..9301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9217..9236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9271..9290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9341..9476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9496..9515)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9497..9515)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9525..9542)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9525..9541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9534..9556)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9683..10138
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9770..9789)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9980..10001
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10164..10268
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10269..10337,10338..11129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11300..11888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11789..11808
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12042..12059
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12176..12197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12212..12217,12218..12235,12236..12242)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12250..12266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12255..12277
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12274..12290
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12274..12290
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12309..12329
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12311..12329
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag agccgggatc ccgcccagcc
     4141 gatgtcgcct ggagaagcga cgcagagtgg cgcccggcct gccgaccgct atggcctatt
     4201 gaagcacagt cgcgagttct tggacttctt ctgggacatt gcgaagcctg agcaggagac
     4261 gcgacttgcg gccacggaga agctgctgga gtatctgcgt ggcaggccga aggggtccga
     4321 gatgaaatat gccctgaagc gtctaatcac gggactcggg gtcgggcgag aaacagcccg
     4381 gccctgctac agtttggccc tggcacagct gttacagtct tttgaagacc tccccttgtg
     4441 cagcatcctg cagcagatac aagaaaaata tgacctgcat caggtgaaga aggcaatgct
     4501 gagacctgct ctctttgcaa acctgtttgg agtgctcgcc ctctttcagt caggtcggct
     4561 ggtgaaggac caggaggcac tgatgaagtc ggtgaagctg ctgcaggccc tggcccagta
     4621 ccaaaaccac ttgcaggagc agccccggaa ggccctggtg gacatcctct ccgaggtctc
     4681 gaaggccaca ttgcaggaga tcctgccgga ggtcctcaaa gccgacttga atataatact
     4741 cagctcccct gaacagctag agctcttcct cctggcccag cagaaggtgc cctccaagct
     4801 caagaagctg gtgggatccg tgaacctatt ctcagatgag aatgtcccca ggctggtgaa
     4861 tgtgctgaag atggccgcct cctctgtgaa gaaggaccgc aagctgcccg ccattgctct
     4921 ggacctgctc cgcctggcac tcaaggaaga caagttccca cggttctgga aggaggtggt
     4981 ggaacaaggg ctgctgaaga tgcagttctg gccagccagc tacctgtgtt tccgcctgct
     5041 gggcgcggcc ctgcccctgc tgaccaagga gcagctgcac ctggtgatgc agggagacgt
     5101 gatccgccat tacggggagc acgtgtgcac tgctaagctc ccaaagcagt tcaagtttgc
     5161 cccagagatg gacgattacg tgggcacctt cctagagggg tgccaggatg accctgagcg
     5221 gcagctggcc gtgctagtgg ccttctcatc tgtcaccaac caaggcctcc ctgtcacgcc
     5281 tactttctgg cgggtcgtgc ggttcctgag ccctccggcc ctgcagggct atgtggcctg
     5341 gctgcgggcc atgtttctcc agccagacct ggactccttg gttgacttca gcaccaacaa
     5401 ccagaagaaa gcccaggatt catcgctcca catgcctgag cgagctgtgt tccggctgag
     5461 gaaatggatc atctttcgat tggtgagcat tgtggacagc ctgcacctgg agatggagga
     5521 ggccttgact gagcaggtgg ccaggttttg tttgttccac tcgttctttg tcacaaagaa
     5581 gcccacatcc cagatccctg agacaaagca cccgttctcc ttccctttgg aaaaccaggc
     5641 ccgagaggct gtcagcagtg ccttcttcag tctgttgcag accctcagca cgcagttcaa
     5701 gcaggcaccg ggccagaccc agggtgggca gccctggacc taccacctgg tgcagttcgc
     5761 agacctcctg ttgaatcaca gccacaacgt gaccaccgtg acacccttca ctgcgcagca
     5821 gcgccaggcc tgggaccgga tgctgcagac tctgaaggag ctggaggccc actccgcaga
     5881 ggccagggct gctgccttcc agcaccttct gctcctcgtg ggcatccacc tcctcaagtc
     5941 ccctgcagag agctgtgacc tgctgggtga catccagacc tgcatcagga aaagtctggg
     6001 agagaagccc cgccggagcc gcaccaagac catcgacccc caggaacccc cgtgggtaga
     6061 ggtgctggtg gagatcttgc tggccctgtt ggcccagccc agccacctca tgcgccaggt
     6121 ggcccggagc gtgtttggcc acatctgctc ccacctgacc ccgcgtgccc tgcagctaat
     6181 tctggatgtg ctgaaccccg agaccagtga ggatgagaat gaccgtgtgg tggtgacgga
     6241 cgattctgat gagcggcggc tgaagggtgc agaggacaag agcgaggaag gtgaggacaa
     6301 cagaagctca gagagtgaag aggagagcga gggggaggag agcgaggagg aggagcgcga
     6361 cggggacgtg gatcagggct tccgggaaca gctgatgacc gtgctgcagg ctgggaaggc
     6421 gctgggtgga gaggacagtg agaacgagga ggagctgggg gatgaggcca tgatggccct
     6481 ggaccagagc ctcgccagcc tctttgccga gcagaagctg cgtatccagg cccggcgaga
     6541 cgagaagaac aagctgcaga aggagaaggc tctgcggcgc gacttccaga tccgggtgct
     6601 ggacctggtg gaggtgctag tgaccaagca gcccgagaat gccctggtcc tggagctgct
     6661 ggagccgctg ctgagcatca tccggcgcag cctgcgcagc agcagctcca aacaggagca
     6721 ggaccttctg cacaagacgg cgcgcatctt cacgcaccac ctgtgccgtg cccggcgcta
     6781 ctgccacgac ttgggtgagc gcgcaggggc cctgcacgcc caggtggagc ggttggtgca
     6841 gcaggctggc cgccagcccg actcccccac cgccctctac cacttcaacg cctctctcta
     6901 cctgctccgg gtcttgaagg gcaacactgc tgagggctgc gtgcatgaga cacaggagaa
     6961 gcagaaagct ggcactgacc ccagccacat gcccacgggc ccgcaggctg ccagctgctt
     7021 ggacttgaac ctggtgaccc gggtgtactc gacagcactg agctccttcc tgaccaagcg
     7081 caacagcccc ctcacagttc ccatgttcct cagcctcttc tcccggcacc cggtgctctg
     7141 tcagagcctg ctccccatcc tggtccagca tatcacgggc ccggtgcggc cccgtcatca
     7201 ggcctgcctg ctgctccaga agaccctgtc catgcgggag gtgaggtcgt gctttgagga
     7261 ccccgagtgg aagcagctga tgggccaggt cctagcaaag gtcaccgaga acttgcgcgt
     7321 gctgggggag gcgcagacca aggcgcagca tcagcaggca ctgtcctccc tggagctgct
     7381 caacgttctc ttcaggacct gcaaacatga gaagctgacc ttggacctga cggtgctcct
     7441 gggtgtgctg caggggcaac agcagagcct acagcagggg gcacactcca ccggctccag
     7501 ccgcctgcac gacctctact ggcaggccat gaaaaccctg ggagtccagc gccccaagtt
     7561 ggagaagaag gatgccaagg agatccccag tgccacccag agccccatca gtaagaagcg
     7621 gaagaaaaag ggattcttgc cagagacgaa gaagcgcaag aaacgcaagt cagaggatgg
     7681 cacgccagcg gaggatggca cacctgcagc caccggcggg agccagcccc ccagcatggg
     7741 caggaagaag aggaacagga caaaggctaa ggtcccagcc caggcaaacg ggacgccaac
     7801 caccaagagt ccagcccctg gcgcccccac ccggagcccc agcacccctg ccaaatcccc
     7861 aaaactgcag aagaaaaacc agaagccgtc ccaggtgaat ggagctcccg ggtcccccac
     7921 ggaacctgca ggccaaaagc agcatcagaa ggctcttccc aaaaaggggg tcttgggcaa
     7981 atcaccactg tccgcgctgg cacggaaaaa ggcaaggctg tctttggtca tcaggagtcc
     8041 cagcctgctt cagagtgggg ccaagaagaa agcacaggtg aggaaggcag ggaagcccta
     8101 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     8161 aaaggacgaa taatggttat acccgtagat gtgacgcgtt aagtcgacaa tcaacctctg
     8221 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8281 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8341 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8401 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8461 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     8521 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     8581 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     8641 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     8701 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     8761 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     8821 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8881 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8941 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9001 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9061 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     9121 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     9181 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     9241 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     9301 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     9361 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9421 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9481 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     9541 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     9601 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     9661 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9721 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9781 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9841 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9901 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9961 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    10021 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    10081 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
    10141 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10201 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10261 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10321 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10381 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10441 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10501 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10561 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10621 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10681 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10741 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10801 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10861 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10921 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10981 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    11041 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    11101 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    11161 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11221 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11281 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11341 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11401 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11461 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11521 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11581 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11641 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11701 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11761 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11821 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11881 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11941 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    12001 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    12061 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    12121 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    12181 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    12241 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    12301 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
