LOCUS       sequence-397227-       11014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2501
FEATURES             Location/Qualifiers
     source          1..11014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6871..7459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6924..6944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7342..7353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7531..7764
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7842..7963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7879..7898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7933..7952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8003..8138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8158..8177)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8159..8177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8187..8204)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8187..8203)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8196..8218)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8345..8800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8432..8451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8642..8663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8826..8930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8931..8999,9000..9791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9149..9168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9962..10550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10451..10470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10704..10721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10838..10859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10874..10879,10880..10897,10898..10904)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10912..10928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10917..10939
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10936..10952
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10936..10952
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10971..10991
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10973..10991
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag cctgcacccg cccgatctcc
     4141 gaggccccag caggaccccg cccggcccca ggagcccacc atgcctcccc ccgagacccc
     4201 ctctgaaggc cgccagccca gccccagccc cagccctaca gagcgagccc ccgcttcgga
     4261 ggaggagttc cagtttctgc gctgccagca atgccaggcg gaagccaagt gcccgaagct
     4321 gctgccttgt ctgcacacgc tgtgctcagg atgcctggag gcgtcgggca tgcagtgccc
     4381 catctgccag gcgccctggc ccctaggtgc agacacaccc gccctggata acgtcttttt
     4441 cgagagtctg cagcggcgcc tgtcggtgta ccggcagatt gtggatgcgc aggctgtgtg
     4501 cacccgctgc aaagagtcgg ccgacttctg gtgctttgag tgcgagcagc tcctctgcgc
     4561 caagtgcttc gaggcacacc agtggttcct caagcacgag gcccggcccc tagcagagct
     4621 gcgcaaccag tcggtgcgtg agttcctgga cggcacccgc aagaccaaca acatcttctg
     4681 ctccaacccc aaccaccgca cccctacgct gaccagcatc tactgccgag gatgttccaa
     4741 gccgctgtgc tgctcgtgcg cgctccttga cagcagccac agtgagctca agtgcgacat
     4801 cagcgcagag atccagcagc gacaggagga gctggacgcc atgacgcagg cgctgcagga
     4861 gcaggatagt gcctttggcg cggttcacgc gcagatgcac gcggccgtcg gccagctggg
     4921 ccgcgcgcgt gccgagaccg aggagctgat ccgcgagcgc gtgcgccagg tggtagctca
     4981 cgtgcgggct caggagcgcg agctgctgga ggctgtggac gcgcggtacc agcgcgacta
     5041 cgaggagatg gccagtcggc tgggccgcct ggatgctgtg ctgcagcgca tccgcacggg
     5101 cagcgcgctg gtgcagagga tgaagtgcta cgcctcggac caggaggtgc tggacatgca
     5161 cggtttcctg cgccaggcgc tctgccgcct gcgccaggag gagccccaga gcctgcaagc
     5221 tgccgtgcgc accgatggct tcgacgagtt caaggtgcgc ctgcaggacc tcagctcttg
     5281 catcacccag gggaaagatg cagctgtatc caagaaagcc agcccagagg ctgccagcac
     5341 tcccagggac cctattgacg ttgacctgcc cgaggaggca gagagagtga aggcccaggt
     5401 tcaggccctg gggctggctg aagcccagcc tatggctgtg gtacagtcag tgcccggggc
     5461 acaccccgtg ccagtgtacg ccttctccat caaaggccct tcctatggag aggatgtctc
     5521 caatacaacg acagcccaga agaggaagtg cagccagacc cagtgcccca ggaaggtcat
     5581 caagatggag tctgaggagg ggaaggaggc aaggttggct cggagctccc cggagcagcc
     5641 caggcccagc acctccaagg cagtctcacc accccacctg gatggaccgc ctagccccag
     5701 gagccccgtc ataggaagtg aggtcttcct gcccaacagc aaccacgtgg ccagtggcgc
     5761 cggggaggca gaggaacgcg ttgtggtgat cagcagctcg gaagactcag atgccgaaaa
     5821 ctcgtcctcc cgagagctgg atgacagcag cagtgagtcc agtgacctcc agctggaagg
     5881 ccccagcacc ctcagggtcc tggacgagaa ccttgctgac ccccaagcag aagacagacc
     5941 tctggttttc tttgacctca agattgacaa tgaaacccag aagattagcc agctggctgc
     6001 ggtgaaccgg gaaagcaagt tccgcgtggt catccagcct gaagccttct tcagcatcta
     6061 ctccaaggcc gtgtccctgg aggtggggct gcagcacttc ctcagctttc tgagctccat
     6121 gcgccgccct atcttggcct gctacaagct gtgggggcct ggcctcccaa acttcttccg
     6181 ggccctggag gacattaaca ggctgtggga attccaggag gccatctcgg gcttcctggc
     6241 tgccctgcct ctcatccggg agcgtgtgcc cggggccagc agcttcaaac tcaagaacct
     6301 ggcccagacc tacctggcga gaaacatgag cgagcgcagc gccatggctg ccgtgctggc
     6361 catgcgtgac ctgtgccgcc tcctcgaggt ctccccgggc ccccagctgg cccagcatgt
     6421 ctaccccttc agtagcctgc agtgctttgc ctccctgcag cccctggtgc aggcagctgt
     6481 gctgccccgg gctgaggccc gcctcctggc cctacacaac gtgagcttca tggagctgct
     6541 gagtgcacac cgccgtgacc ggcagggggg cctgaagaag tacagccgct atctaagcct
     6601 gcagaccacc acgttgcccc ctgcccagcc tgctttcaac ctgcaggctc tgggcaccta
     6661 ctttgaaggc ctgttggagg gtccggcgct ggcacgggca gaaggagtct ccaccccact
     6721 tgctggccgt ggcttggcag agagggcctc ccagcagagc tagtaatgaa ctagtccgta
     6781 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg acctggagat
     6841 aggttaagca cgttaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     6901 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6961 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7021 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7081 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7141 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7201 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7261 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7321 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7381 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7441 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     7501 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7561 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7741 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     7801 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     7861 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7921 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7981 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8041 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     8101 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     8161 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     8221 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     8281 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     8341 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8401 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8461 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8521 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8581 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8641 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8701 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8761 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     8821 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8881 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8941 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9001 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     9061 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     9121 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     9181 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9241 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9301 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9361 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9421 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9481 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9541 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9601 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9661 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9721 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9781 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9841 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9901 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9961 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10021 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    10081 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    10141 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    10201 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    10261 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    10321 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    10381 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10441 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10501 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10561 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10621 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10681 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    10741 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    10801 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    10861 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    10921 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    10981 ctcactaaag ggaacaaaag ctggagctgc aagc
//
