LOCUS       sequence-397285-       11305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2668
FEATURES             Location/Qualifiers
     source          1..11305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7162..7750
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7215..7235)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7633..7644)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7822..8055
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8133..8254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8170..8189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8224..8243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8294..8429
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8449..8468)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8450..8468)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8478..8495)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8478..8494)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8487..8509)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8636..9091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8723..8742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8933..8954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9117..9221
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9222..9290,9291..10082)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9440..9459)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10253..10841
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10742..10761
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10995..11012
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11129..11150
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11165..11170,11171..11188,11189..11195)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11203..11219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11208..11230
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11227..11243
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11227..11243
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11262..11282
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11264..11282
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgagt gtatgtactc taagtgtacc
     4141 agtttcttca ctctctcctg gcagaagatg caacactttt agtgattctg ggattctggg
     4201 atgtgttcct attaattcta atacagatga agaagatgtg gtagaggaaa agatggtagc
     4261 agaaggagtg aataaagagg caaaacagcc tgctaaaaag aaaagaaaga agggtttgcg
     4321 aattaagggg aaaaggcgtc gaaaaaaatt gattcttgcc aaaaagttta gtaaggattt
     4381 gggatctggg aggcctgttg cagatgcccc tgctttgtta gcttccaatg accctgagca
     4441 ggatgaagaa agtctttttg agagcaatat agaaaaacag atctatctac ctagtactag
     4501 agccaagacc tccattgtgt ggcacttctt tcatgttgac ccccagtaca cctggcgggc
     4561 catttgtaac ctctgtgaga aaagcgtcag caggggtaaa ccaggtagcc atcttggtac
     4621 atctactctt caacgacatc tgcaagcaag gcattcacct cattggacca gggccaacaa
     4681 gtttggagtt gctagtggag aggaggactt taccttggat gtgtctttat ctccctcttc
     4741 tggaagcaat ggaagctttg aatatattcc tactgatcca ttagatgata atagaatggg
     4801 caagaagcat gataaatcag catctgatgc cctaagggca gaaagaggga gatttctcat
     4861 caaaagtaac attgtcaagc atgctttaat tcctggaact agagccaaga catctgccgt
     4921 ttggaatttt ttttacactg atcctcagca catctcaaga gctgtgtgta atatatgtaa
     4981 aagaagtgtg agccggggta ggccagggtc ccacttaggg acttcaacac ttcaacgaca
     5041 cctgcaagcc acacatccta tccattgggc tgttgccaac aaagacagtg gtgctgttgc
     5101 aaatggatta gatgaagctg agactgagag aagtgatctc ttgagtgata ccttgcatgg
     5161 agaaaagtct acaggcagcc aagatttaac agctgaggac cttagtgact ctgattcaga
     5221 tgaacctatg ttagaggttg aaaacagatc tgaaagtcct attcccgttg cagagcaagg
     5281 cactctgatg cgtgcgcagg agagagaaac aacatgttgt ggaaatccag tctcaagtca
     5341 cataagtcag gcaattatcc aaatgattgt ggaggatatg catccttaca actatttctc
     5401 aaccccagcc tttcagaggt tcatgcagat tgtggcccct gattataggt tgccatcaga
     5461 gacttacttt ttcactaagg ctgtacctca gttatatgat tgtgtcagag aaaaaatttt
     5521 cttaacttta gagaatgttc aaagccaaaa gatacacctg actgttgaca tatggaccca
     5581 tgacccatcc actgactatt ttattgtgac tgtacactgg gtttctttgg aaactgcgtc
     5641 ttttctcaat aatggcagga tccccgattt tagaaagtgg gcagtgcttt gtgttacagg
     5701 tttggctaaa gactgtttga taaccaatat tttacaagaa ttaaatgacc agattggtct
     5761 gtggctttct ccaaatttcc ttatccctag cttcattgtt tctgacaatt cctctaatgt
     5821 ggtacatgca atcaaagatg gtggttttac ccatgtgcca tgcttcctgc attgtttaaa
     5881 tatggtcatt caggactttt tctgcgagca caaaagcatt gagaatatgt tagtggctgc
     5941 caggaaaact tgtcatcatt ttagtcattc ggtcaaggcc cgtcagatac tgcaagagtt
     6001 ccaaaatgat caccaacttc catggaagaa tttgaagcag gacgaaactg gccattggat
     6061 ttctactttt tatatgttaa aatggctctt ggagcattgc tactcagttc accatagtct
     6121 tggtagagcc agtggagttg tgctcacctc ccttcagtgg actctaatga cttatgtttg
     6181 tgatattctt aagccatttg aggaggctac ccagaaagtg agcgtgaaga ccgcaggatt
     6241 gaatcaggtg ctacccctaa tccatcatct actcctttcc ctgcagaaac tcagagaaga
     6301 ttttcaagtc agaggtatta ctcaggccct caatctggtg gatagtttat ctctgaaact
     6361 tgaaacagat accctactaa gtgccatgct taaatccaag ccctgtatct tggctacttt
     6421 gttagatcct tgctttaaaa acagtttgga agactttttt cctcaaggtg ctgatttaga
     6481 aacttataag cagttccttg cagaagaggt ctgtaattat atggaatctt caccagagat
     6541 ctgccaaatt ccaacttcag aagcttcttg tccctcagtt acagtgggag ctgattcatt
     6601 tacctcatct ctaaaagaag gcacctccag ttcaggttct gttgatagct cagctgtaga
     6661 caatgttgcc cttggaagca aaagcttcat gttcccttct gctgtagcag ttgtggatga
     6721 gtacttcaaa gagaagtatt cagagttctc aggaggtgat gaccctttaa tttactggca
     6781 gaggaagata agcatatggc cggctttgac ccaggttgcc atccagtatc taagttgccc
     6841 catgtgtagt tggcaatctg aatgtatctt tactaaaaat agccactttc atccaaaaca
     6901 gatcatgagc ctggactttg acaatataga acaactgatg tttctgaaaa tgaacttgaa
     6961 aaatgttaac tacgattatt ctacgttggt tctgagctgg gatcctgagc agaatgaagt
     7021 tgttcaaagc agtgaaaaag aaatactgcc ttagtaatga actagtccgt aacttgaaag
     7081 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaatggtcat cacgatctta
     7141 aataggacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     7201 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7261 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7321 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7381 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7441 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7501 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7561 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7621 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7681 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7741 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     7801 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     7861 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7921 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7981 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8041 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     8101 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8161 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8221 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8281 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8341 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8401 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     8461 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8521 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8581 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     8641 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8701 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8761 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8821 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8881 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8941 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     9001 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9061 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     9121 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     9181 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     9241 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     9301 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     9361 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     9421 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     9481 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     9541 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     9601 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     9661 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9721 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9781 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9841 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9901 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9961 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
    10021 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
    10081 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
    10141 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
    10201 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
    10261 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
    10321 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
    10381 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
    10441 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
    10501 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
    10561 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
    10621 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
    10681 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10741 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10801 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10861 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10921 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10981 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    11041 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    11101 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    11161 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    11221 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    11281 gggaacaaaa gctggagctg caagc
//
