LOCUS       sequence-397292-       10780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2535
FEATURES             Location/Qualifiers
     source          1..10780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6637..7225
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6690..6710)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7108..7119)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7297..7530
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7608..7729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7645..7664)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7699..7718
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7769..7904
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7924..7943)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7925..7943)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7953..7970)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7953..7969)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7962..7984)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8111..8566
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8198..8217)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8408..8429
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8592..8696
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8697..8765,8766..9557)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8915..8934)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9728..10316
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10217..10236
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10470..10487
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10604..10625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10640..10645,10646..10663,10664..10670)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10678..10694
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10683..10705
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10702..10718
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10702..10718
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10737..10757
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10739..10757
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggga agaatgtctt ccaagcaagc
     4141 cacctctcca tttgcctgtg cagctgatgg agaggatgca atgacccagg atttaacctc
     4201 aagggaaaag gaagagggca gtgatcaaca tgtggcctcc catctgcctc tgcaccccat
     4261 aatgcacaac aaacctcact ctgaggagct accaacactt gtcagtacca ttcaacaaga
     4321 tgctgactgg gacagcgttc tgtcatctca gcaaagaatg gaatcagaga ataataagtt
     4381 atgttcccta tattccttcc gaaatacctc tacctcacca cataagcctg acgaagggag
     4441 tcgggaccgt gagataatga ccagtgttac ttttggaacc ccagagcgcc gcaaagggag
     4501 tcttgccgat gtggtggaca cactgaaaca gaagaagctt gaggaaatga ctcggactga
     4561 acaagaggat tcctcctgca tggaaaaact actttcaaaa gattggaagg aaaaaatgga
     4621 aagactaaat accagtgaac ttcttggaga aattaaaggt acacctgaga gcctggcaga
     4681 aaaagaacgg cagctctcca ccatgattac ccagctgatc agtttacggg agcagctact
     4741 ggcagcgcat gatgaacaga aaaaactggc agcgtcacaa attgagaaac aacggcagca
     4801 aatggacctt gctcgccaac agcaagaaca gattgcgaga caacagcagc aacttctgca
     4861 acagcagcac aaaattaatc tcctgcagca acagatccag gttcagggtc acatgcctcc
     4921 gctcatgatc ccaatttttc cacatgacca gcggactctg gcagcagctg ctgctgccca
     4981 acagggattc ctcttccccc ctggaataac atacaaacca ggtgataact accccgtaca
     5041 gttcattcca tcaacaatgg cagctgctgc tgcttctgga ctcagccctt tacagctcca
     5101 gcagctctat gccgctcagc tggccagcat gcaggtgtca cctggagcaa agatgccatc
     5161 aactccacag ccaccaaaca cagcagggac ggtctcacct actgggataa aaaatgaaaa
     5221 gagagggacc agccctgtaa ctcaagttaa ggatgaagca gcagcacagc ctctgaatct
     5281 ctcatcccga cccaagacag cagagcctgt aaagtcccca acgtctccca cccagaacct
     5341 cttcccagcc agcaaaacca gccctgtcaa tctgccaaac aaaagcagca tccctagccc
     5401 cattggagga agcctgggaa gaggatcctc tttaggtaaa tggaaaagtc aacaccagga
     5461 agagacttac gaattagata tcctatctag tctcaactcc cctgcccttt ttggggatca
     5521 ggatacagtg atgaaagcca ttcaggaggc gcggaagatg cgagagcaga tccagcggga
     5581 gcaacagcag caacagccac atggtgttga cgggaaactg tcctccataa ataatatggg
     5641 gctgaacagc tgcaggaatg aaaaggaaag aacgcgcttt gagaatttgg ggccccagtt
     5701 aacgggaaag tcaaatgaag atggaaaact gggcccaggt gtcatcgacc ttactcggcc
     5761 agaagatgca gagggaagta aagcaatgaa tggctctgca gctaaactac agcagtatta
     5821 ttgttggcca acaggaggtg ccactgtggc tgaagcacga gtctacaggg acgcccgcgg
     5881 ccgtgccagc agcgagccac acattaagcg accaatgaat gcattcatgg tttgggcaaa
     5941 ggatgagagg agaaaaatcc ttcaggcctt ccccgacatg cataactcca acattagcaa
     6001 aatcttagga tctcgctgga aatcaatgtc caaccaggag aagcaacctt attatgaaga
     6061 gcaggcccgg ctaagcaaga tccacttaga gaagtaccca aactataaat acaaaccccg
     6121 accgaaacgc acctgcattg ttgatggcaa aaagcttcgg attggggagt ataagcaact
     6181 gatgaggtct cggagacagg agatgaggca gttctttact gtggggcaac agcctcagat
     6241 tccaatcacc acaggaacag gtgttgtgta tcctggtgct atcactatgg caactaccac
     6301 accatcgcct cagatgacat ctgactgctc tagcacctcg gccagcccgg agcccagcct
     6361 cccggtcatc cagagcactt atggtatgaa gacagatggc ggaagcctag ctggaaatga
     6421 aatgatcaat ggagaggatg aaatggaaat gtatgatgac tatgaagatg accccaaatc
     6481 agactatagc agtgaaaatg aagccccgga ggctgtcagt gccaactagt aatgaactag
     6541 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaccc
     6601 agagcctctc cacagataga cacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     6661 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6721 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6781 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6841 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6901 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6961 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7021 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7081 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7141 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7201 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     7261 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7321 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7381 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7441 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7501 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     7561 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     7621 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7681 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     7741 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7801 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7861 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     7921 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     7981 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     8041 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     8101 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     8161 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     8221 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8281 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8341 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8401 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     8461 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     8521 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     8581 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8641 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8701 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8761 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8821 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8881 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8941 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9001 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     9061 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     9121 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     9181 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     9241 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9301 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9361 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9421 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9481 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9541 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9601 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9661 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9721 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9781 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9841 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9901 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9961 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    10021 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    10081 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    10141 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10201 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10261 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10321 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10381 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10441 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10501 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    10561 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    10621 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    10681 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    10741 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
//
