LOCUS       sequence-397303-       11572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2452
FEATURES             Location/Qualifiers
     source          1..11572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7429..8017
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7482..7502)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7900..7911)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8089..8322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8400..8521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8437..8456)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8491..8510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8561..8696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8716..8735)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8717..8735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8745..8762)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8745..8761)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8754..8776)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8903..9358
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8990..9009)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9200..9221
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9384..9488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9489..9557,9558..10349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9707..9726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10520..11108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11009..11028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11262..11279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11396..11417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11432..11437,11438..11455,11456..11462)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11470..11486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11475..11497
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11494..11510
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11494..11510
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11529..11549
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11531..11549
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggtc tctagcgacc ggcccgtgtc
     4141 actggaggac gaggtctccc atagtatgaa ggagatgatt ggaggctgtt gcgtttgctc
     4201 agacgagaga ggctgggccg agaacccgct ggtttattgc gacgggcacg gctgcagcgt
     4261 cgcggtgcat caagcttgct atggcattgt tcaagtaccc actggaccgt ggttttgcag
     4321 gaaatgtgaa tctcaggaga gagcagccag agtgagatgt gaactttgtc cccataagga
     4381 tggagcttta aaaagaacag ataatggggg ttgggcccat gtggtttgtg ccctgtatat
     4441 tccagaggta caatttgcca atgtttccac aatggaacca attgttttac agtctgttcc
     4501 gcatgatcgt tataataaga cttgctacat ttgtgatgaa caaggaagag aaagcaaagc
     4561 agccactggt gcttgcatga catgtaataa acatggatgt cgacaggctt tccatgtaac
     4621 atgcgctcag tttgccggac tgctttgtga agaagaaggt aatggtgccg ataatgtcca
     4681 atactgtggc tactgtaaat accattttag taagctgaaa aagagcaaac ggggatctaa
     4741 taggtcatat gatcaaagtt taagtgattc ttcctctcac tctcaggata aacatcatga
     4801 gaaagagaaa aaaaaatata aagagaagga caaacacaaa cagaaacaca agaagcagcc
     4861 agaaccatca cctgcattgg ttccatcctt gactgttact acagaaaaaa cttatacaag
     4921 cactagcaac aactctatat ctggatcatt gaagcgcttg gaagatacta ctgcacgatt
     4981 tacaaatgca aatttccagg aagtctctgc acacacctct agtggaaaag atgtttcaga
     5041 gactagaggg tcagagggca aagggaagaa atcttcagct cacagctcag gtcaaagggg
     5101 aagaaagcct ggtggtggaa gaaatccagg aacaactgtg tcagcagcta gcccttttcc
     5161 tcaaggcagt ttttcaggaa ctccaggcag tgtaaagtca tcttctggaa gttcagtgca
     5221 gtctccccag gatttcctga gctttacaga ctcagatctg cgtaatgaca gttactctca
     5281 ctcccaacag tcatcagcaa ccaaagatgt acataaagga gagtctggaa gccaggaagg
     5341 gggggtaaat agttttagta ccttaattgg cctcccttca acctcagctg ttacttcaca
     5401 gcctaaaagc tttgaaaatt cacctggaga tttgggtaat tccagccttc ctacagcagg
     5461 atataagcgg gctcaaactt ctggcataga agaagaaact gtaaaggaaa agaaaaggaa
     5521 aggaaataaa caaagtaagc atgggcctgg cagacccaaa ggaaacaaaa atcaagagaa
     5581 tgtttctcat ctctcagttt cttctgcttc accaacatca tctgtagcat cagctgcagg
     5641 aagcataaca agctctagtc tgcagaaatc tcctacattg ctcaggaatg gaagtttaca
     5701 gagcctcagt gttggctcat ctccagttgg ttcagaaatt tccatgcagt atcggcatga
     5761 tggagcttgc ccaacaacta cgttctcaga gttgctgaat gcaatacaca acggtattta
     5821 taacagcaat gatgtagcag tatcgtttcc aaatgtagta tctggctcgg gatctagtac
     5881 tcctgtctcc agctctcact tacctcagca gtcttctggg catttgcaac aagtaggagc
     5941 gctctctccc tcagctgtgt catctgcagc ccctgctgtt gctacaactc aggcaaatac
     6001 tctatctgga tcttctctca gtcaggcacc atctcatatg tatggcaata gatcaaattc
     6061 atcaatggca gctcttatag ctcagtctga aaacaatcaa acagatcaag atcttggaga
     6121 caatagccgc aacctagttg gcagaggaag ctcaccccga ggaagtctct cgccacgatc
     6181 ccctgtaagc agcttacaga ttcgctatga tcaaccaggc aacagcagtt tggaaaatct
     6241 gcctccagta gcagccagca tagaacagct tttggagagg cagtggagtg aaggacagca
     6301 atttttacta gaacagggta ctcctagtga cattttagga atgctgaagt cattacacca
     6361 acttcaagtt gaaaaccgaa gattagagga acaaattaaa aacttgactg ccaaaaagga
     6421 acggcttcag ttattgaatg cacagctttc agtgcctttt ccaacaataa cagcaaatcc
     6481 tagtccgtct catcaaatac acacattttc agcacagact gctcctacta ctgattcctt
     6541 gaacagcagt aagagccctc atataggaaa cagcttttta cctgataatt ctcttcctgt
     6601 attaaatcag gacttaacct ccagtggaca aagtaccagc agctcatcag ctctttctac
     6661 cccacctcct gctgggcaga gtccggctca acaaggctca ggagtgagtg gagttcagca
     6721 ggtcaatggc gtgacagtgg gggcactagc tagtggaatg cagcctgtaa cttccaccat
     6781 tcctgccgtg tctgcagtgg gtggaataat tggagctttg ccaggtaacc aactggcaat
     6841 taatggcatt gtaggagctt taaatggggt tatgcagact cctgtcacaa tgtcccagaa
     6901 ccctacccct ctcacccaca caaccgtacc acctaatgca acacatccaa tgccagctac
     6961 actgactaac agtgcctcag gactaggatt actttctgac cagcaacgac aaatacttat
     7021 tcatcaacag cagtttcagc agttgttaaa ttctcaacag ctcacaccag aacaacatca
     7081 agcctttttg tatcagttaa tgcaacatca ccaccagcag caccaccaac ctgaacttca
     7141 gcagctgcag atccctggac caacacaaat acccataaac aaccttcttg caggtacaca
     7201 ggcaccccca cttcacacag ctaccaccaa cccatttctc accatccatg gagataatgc
     7261 aagtcagaaa gtagcaagac ttagtgataa aactgggcct gtagctcaag agaaaagtta
     7321 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     7381 aaaggacgaa gcctcctcat cttacggtac tccacgcgtt aagtcgacaa tcaacctctg
     7441 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     7501 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     7561 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     7621 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     7681 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     7741 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7801 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7861 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7921 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7981 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     8041 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8101 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8161 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8221 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8281 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     8341 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     8401 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8461 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8521 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     8581 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8641 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8701 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8761 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8821 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8881 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8941 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9001 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9061 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9121 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9181 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9241 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9301 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9361 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9421 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9481 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9541 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9601 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9661 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9721 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9781 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9841 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9901 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9961 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10021 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10081 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10141 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10201 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10261 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10321 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10381 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10441 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11161 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11221 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11281 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11341 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11401 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11461 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    11521 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gc
//
