LOCUS       sequence-397349-       12102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac Vector expressing TEX264-Delta-LIR-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo 
            JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, 
            Harper JW
  TITLE     Combinatorial selective ER-phagy remodels the ER during 
            neurogenesis.
  JOURNAL   Nat Cell Biol. 2024 Mar;26(3):378-392. doi: 
            10.1038/s41556-024-01356-4. Epub 2024 Mar 1.
  PUBMED    38429475
REFERENCE   2  (bases 1 to 12102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01356-4"; journalName: "Nat Cell Biol"; date: 
            "2024-03"; volume: "26"; issue: "3"; pages: "378-392"
COMMENT     Sequence Label: pAC150-TEX264-Delta-LIR-GFP
FEATURES             Location/Qualifiers
     source          1..12102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   1069..1103
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1437..1470
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        1620..1999
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2000..2203
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2154..2174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2200..2224
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3388..4383
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    4419..4553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4456..4475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4510..4529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    4582..4615
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4976..6154
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5207..6145
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6102..6122
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    6215..6239
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(7219..7221,7222..7935)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7219..7221,7222..7932)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7261..7282)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7522..7541)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(7938..7962)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8015..8056
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(8094..8111)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8100..8324
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   9144..9206
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9244..9263)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9245..9263)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9270..9287)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9270..9286)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9279..9301)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9424..9726
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9605..9624
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             10075..10869
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10075..10869
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10129..10148)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10739..10758
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(11119..11910,11911..11979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11742..11761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttttaacc ctagaaagat
     1081 agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc taaatagcgc
     1141 gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc gtgaggcgtg
     1201 cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga gtcaaaatga
     1261 cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt atattgttat
     1321 ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat agatatcaac
     1381 tagaatgcta gacctttcgt cttcaagaat tccgatcata ttcaataacc cttaatataa
     1441 cttcgtataa tgtatgctat acgaagttat taggtctgaa gaggagttta cgtccagcca
     1501 agcttaggat cccgggtacc gatggccggg cctcgaggag cttggcccat tgcatacgtt
     1561 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg
     1621 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     1681 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1741 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1801 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1861 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1921 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1981 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2041 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2101 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2161 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2221 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     2281 cagcctcccc ggctagccgc cgccaccatg aaaaagcctg aactcaccgc gacgtctgtc
     2341 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     2401 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     2461 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     2521 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     2581 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     2641 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     2701 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     2761 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     2821 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     2881 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     2941 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     3001 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     3061 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     3121 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     3181 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     3241 agaagcgcgg ccgtctggac cgatggctgt gtagaagtcg cgtctgcgtt cgaccaggct
     3301 gcgcgttctc gcggccatag caaccgacgt acggcgttgc gccctcgccg gcagcaagaa
     3361 gccacggaag tccgcccgga gcagaaaatg cccacgctac tgcgggttta tatagacggt
     3421 ccccacggga tggggaaaac caccaccacg caactgctgg tggccctggg ttcgcgcgac
     3481 gatatcgtct acgtacccga gccgatgact tactggcggg tgctgggggc ttccgagaca
     3541 atcgcgaaca tctacaccac acaacaccgc ctcgaccagg gtgagatatc ggccggggac
     3601 gcggcggtgg taatgacaag cgcccagata acaatgggca tgccttatgc cgtgaccgac
     3661 gccgttctgg ctcctcatat cgggggggag gctgggagct cacatgcccc gcccccggcc
     3721 ctcaccctca tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc ggccgcgcgg
     3781 taccttatgg gcagcatgac cccccaggcc gtgctggcgt tcgtggccct catcccgccg
     3841 accttgcccg gcaccaacat cgtgcttggg gcccttccgg aggacagaca catcgaccgc
     3901 ctggccaaac gccagcgccc cggcgagcgg ctggacctgg ctatgctggc tgcgattcgc
     3961 cgcgtttacg ggctacttgc caatacggtg cggtatctgc agtgcggcgg gtcgtggcgg
     4021 gaggactggg gacagctttc ggggacggcc gtgccgcccc agggtgccga gccccagagc
     4081 aacgcgggcc cacgacccca tatcggggac acgttattta ccctgtttcg ggcccccgag
     4141 ttgctggccc ccaacggcga cctgtataac gtgtttgcct gggccttgga cgtcttggcc
     4201 aaacgcctcc gttccatgca cgtctttatc ctggattacg accaatcgcc cgccggctgc
     4261 cgggacgccc tgctgcaact tacctccggg atggtccaga cccacgtcac cacccccggc
     4321 tccataccga cgatatgcga cctggcgcgc acgtttgccc gggagatggg ggaggctaac
     4381 tgaagcttcc cgggggtacc gaattcgtcg acagatctaa cttgtttatt gcagcttata
     4441 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4501 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcatcaagc
     4561 ttaggatccg gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt
     4621 ccctcgaaga ggttcactgg cgcgttggat ccccgggtac cgagttggga gctcacgggg
     4681 acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct agggggcagc
     4741 agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc gagccggcag
     4801 cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct ctgaacgctt
     4861 ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc tttaggctga
     4921 aagagagatt tagaatgaca gaactcgatt tcattgcaga ctggcgcgcc cgtgaggctc
     4981 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     5041 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     5101 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5161 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     5221 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     5281 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     5341 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     5401 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     5461 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     5521 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     5581 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     5641 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     5701 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     5761 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     5821 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     5881 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     5941 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     6001 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     6061 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     6121 tcaaagtttt tttcttccat ttcaggtgtc gtgaagaagc ttggtaccga gctcggatcc
     6181 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctg
     6241 cggtacatgt cggacctgct actactgggc ctgattgggg gcctgactct cttactgctg
     6301 ctgacgctgc tggcctttgc cgggtactca gggctactgg ctggggtgga agtgagtgct
     6361 gggtcacccc ccatccgcaa cgtcactgtg gcctacaagt tccacatggg gctctatggt
     6421 gagactgggc ggcttttcac tgagagctgc agcatctctc ccaagctccg ctccatcgct
     6481 gtctactatg acaaccccca catggtgccc cctgataagt gccgatgtgc cgtgggcagc
     6541 atcctgagtg aaggtgagga atcgccctcc cctgagctca tcgacctcta ccagaaattt
     6601 ggcttcaagg tgttctcctt cccggcaccc agccatgtgg tgacagccac cttcccctac
     6661 accaccattc tgtccatctg gctggctacc cgccgtgtcc atcctgcctt ggacacctac
     6721 atcaaggagc ggaagctgtg tgcctatcct cggctggaga tctaccagga agaccagatc
     6781 catttcatgt gcccactggc acggcaggga gacttctatg tgcctgagat gaaggagaca
     6841 gagtggaaat ggcgggggct tgtggaggcc attgacaccc aggtggatgg cacaggagct
     6901 gacacaatga gtgacacgag ttctgtaagc ttggaagtga gccctggcag ccgggagact
     6961 tcagctgcca cactgtcacc tggggcgagc agccgtggct gggatgacgg tgacacccgc
     7021 agcgagcaca gctacagcga gtcaggtgcc agcggctcct ctgctgagga gctggacttg
     7081 gagggcgagg ggcccttagg ggagtcacgg ctggaccctg ggactgagcc cctggggact
     7141 accaagtggc tctgggagcc cactgcccct gagaagggca aggagggagg ctcaggaggc
     7201 tcaggaggct cactcgaggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     7261 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     7321 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     7381 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     7441 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     7501 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     7561 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     7621 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     7681 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     7741 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     7801 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     7861 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     7921 gagctgtaca agtagtgacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg
     7981 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     8041 cggtctcgat tctacgcgta ccggttaggg gcccgtttaa acccgctgat cagcctcgac
     8101 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     8161 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     8221 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     8281 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcggcgc gcctggatcc
     8341 ccgggtaccg agttgggagc tcacggggac agcccccccc caaagccccc agggatgtaa
     8401 ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg
     8461 cgctcccccc gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg
     8521 gcacgggatc gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg
     8581 gggatacggg gaaaaagctt taggctgaaa gagagattta gaatgacagt ctagtgggag
     8641 ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
     8701 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
     8761 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     8821 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct
     8881 ttaggctgaa agagagattt agaatgacag aactcgattt cattgcagac tggccggcca
     8941 ctagtacgcg ccggctcgac atactagtta aaagttttgt tactttatag aagaaatttt
     9001 gagtttttgt ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt
     9061 attagtatgt aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc
     9121 tcgatataca gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac
     9181 gtcacaatat gattatcttt ctagggttaa aagggcgaat tcgcggccgc taaattcaat
     9241 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     9301 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9361 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctatacgta
     9421 cggcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
     9481 cagagtgata ttattgacac gccggggcga cggatggtga tccccctggc cagtgcacgt
     9541 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
     9601 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
     9661 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
     9721 atataaatgt caggcatgag attatcaaaa aggatcttca cctagatcct tttcacgtag
     9781 aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg
     9841 acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga
     9901 tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc
     9961 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc
    10021 tgatggcgca ggggatcaag ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt
    10081 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
    10141 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
    10201 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
    10261 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
    10321 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
    10381 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
    10441 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
    10501 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
    10561 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
    10621 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
    10681 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
    10741 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
    10801 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
    10861 ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
    10921 tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg gaacccctat
    10981 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagattat caaaaaggat
    11041 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    11101 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    11161 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    11221 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    11281 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    11341 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    11401 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    11461 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    11521 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    11581 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    11641 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    11701 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    11761 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    11821 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    11881 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    11941 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    12001 ttgaagcatt tatcagggtt attgtctcat gaccaaaatc ccttaacgtg agttttcgtt
    12061 ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
