LOCUS       sequence-39735-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Cooper EM, Hudson AW, Amos J, Wagstaff J, Howley PM
  TITLE     Biochemical analysis of Angelman syndrome-associated mutations in 
            the E3 ubiquitin ligase E6-associated protein.
  JOURNAL   J Biol Chem. 2004 Sep 24;279(39):41208-17. Epub 2004 Jul 19.
  PUBMED    15263005
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-09-24"; volume: "279(39)"; pages: "41208-17. 
            Epub 2004 Jul 19"
COMMENT     Sequence Label: p4973 pcDNA4-E6AP III C21Y
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    111..273
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             286..303
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             307..339
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(343..351,352..366)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tttagcagag ctctctggct actagagaac ccactgcctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt agcgcagagg
      121 cttggggcag ccgagcggca gccaggcccc ggcccgggcc tcggttccag aagggagagg
      181 agcccgccaa ggcgcgcaag agagcgggct gcctcgcagt ccgagccgga gagggagcgc
      241 gagccgcgcc ggccccggac ggcctccgaa accatggggg gttctcatca tcatcatcat
      301 catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac
      361 gataaggtac caggatccac catggccaca gcttgtaaaa gatcaggaga acctcagtct
      421 gacgacattg aagctagccg aatgaagcga gcagctgcaa agcatctaat agaacgctac
      481 taccaccagt taactgaggg ctatggaaat gaagcctgca cgaatgagtt ttgtgcttcc
      541 tgtccaactt ttcttcgtat ggataataat gcagcagcta ttaaagccct cgagctttat
      601 aagattaatg caaaactctg tgatcctcat ccctccaaga aaggagcaag ctcagcttac
      661 cttgagaact cg
//
