LOCUS       sequence-39736-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Cooper EM, Hudson AW, Amos J, Wagstaff J, Howley PM
  TITLE     Biochemical analysis of Angelman syndrome-associated mutations in 
            the E3 ubiquitin ligase E6-associated protein.
  JOURNAL   J Biol Chem. 2004 Sep 24;279(39):41208-17. Epub 2004 Jul 19.
  PUBMED    15263005
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-09-24"; volume: "279(39)"; pages: "41208-17. 
            Epub 2004 Jul 19"
COMMENT     Sequence Label: p4978 pcDNA4-E6AP III    E550L
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    106..268
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             281..298
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             302..334
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(338..346,347..361)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tagcgactct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg tttaaactta agcttagcgc agaggcttgg
      121 ggcagccgag cggcagccag gccccggccc gggcctcggt tccagaaggg agaggagccc
      181 gccaaggcgc gcaagagagc gggctgcctc gcagtccgag ccggagaggg agcgcgagcc
      241 gcgccggccc cggacggcct ccgaaaccat ggggggttct catcatcatc atcatcatgg
      301 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
      361 ggtaccagga tccaccatgg ccacagcttg taaaagatca ggagaacctc agtctgacga
      421 cattgaagct agccgaatga agcgagcagc tgcaaagcat ctaatagaac gctactacca
      481 ccagttaact gagggctgtg gaaatgaagc ctgcacgaat gagttttgtg cttcctgtcc
      541 aacttttctt cgtatggata ataatgcagc agctattaaa gccctcgagc tttataagat
      601 taatgcaaaa ctctgtgatc ctcatccctc caagaaagga gcaagctcag cttaccttga
      661 gaactcgaaa ggtgccccca aca
//
