LOCUS       sequence-397382-       10431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for human SFTPC promoter (2kb)-driven expression 
            of EGFP and EF1a-driven TagRFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Lim K, Rutherford EN, Sun D, Van den Boomen DJH, Edgar JR, Bang JH, 
            Matesic LE, Lee JH, Lehner PJ, Marciniak SJ, Rawlins EL, Dickens JA
  TITLE     A novel human fetal lung-derived alveolar organoid model reveals 
            mechanisms of surfactant protein C maturation relevant to 
            interstitial lung disease.
  JOURNAL   bioRxiv [Preprint]. 2023 Sep 4:2023.08.30.555522. doi: 
            10.1101/2023.08.30.555522.
  PUBMED    37693487
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Sep 4:2023.08.30.555522. doi: 
            10.1101/2023.08.30.555522."
COMMENT     Sequence Label: pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP
FEATURES             Location/Qualifiers
     source          1..10431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2218..2442
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2431..2448
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2470..3183)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      3180..3189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             3224..3492
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(3505..3716)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      6088..6097
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6094..6096,6097..6099,6100..6813)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6094..6096,6097..6099,6100..6810)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6139..6160)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6400..6419)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6747..6768
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6821..7409
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6874..6894)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7292..7303)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7484..7717
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7836..7854)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7922..8026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8027..8095,8096..8887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8245..8264)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9058..9646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9547..9566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9800..9817
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10057..10078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10093..10098,10099..10116,10117..10123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10131..10147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10136..10158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10155..10171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10155..10171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10221..10240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt
     2281 cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt
     2341 cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg
     2401 gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct gatcagcgag
     2461 ctctagagct caattaagtt tgtgccccag tttgctaggg aggtcgcagt atctggccac
     2521 agccacctcg tgctgctcga cgtaggtctc tttgtcggcc tccttgattc tttccagtct
     2581 gtggtccaca tagtagacgc cgggcatctt gaggttctta gcgggtttct tggatctgta
     2641 tgtggtcttg aagttgcaga tcaggtggcc cccgcccacg agcttcaggg ccatgtcgct
     2701 tctgccttcc aggccgccgt cagcggggta cagcatctcg gtgttggcct cccagccgag
     2761 tgttttcttc tgcatcacag ggccgttgga tgggaagttc acccctctga tcttgacgtt
     2821 gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca cgcccccgtc
     2881 ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct taaagaagtc
     2941 ggggatgccc tgggtgtggt tgatgaaggt tctgctgccg tacatgaagc tggtagccag
     3001 gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca tggtctgggt
     3061 gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtggtggt tgttcacggt
     3121 gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcttcgc ccttagacac
     3181 catggtggcg ttggctgcag gtgtaggcgc cggtcacagc ttggatctgt aacggcgcag
     3241 aacagaaaac gaaacaaaga cgtagagttg agcaagcagg gtcaggcaaa gcgtggagag
     3301 ccggctgagt ctaggtaggc tccaagggag cgccggacaa aggcccggtc tcgacctgag
     3361 ctttaaactt acctagacgg cggacgcagt tcaggaggca ccacaggcgg gaggcggcag
     3421 aacgcgactc aaccggcgtg gatggcggcc tcaggtaggg cggcgggcgc gtgaaggaga
     3481 gatgcgagcc cctcgaagct tcagctgtgt tctggcggca aacccgttgc gaaaaagaac
     3541 gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag
     3601 ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg cacccgttca
     3661 attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg
     3721 acgggcaccg gagcgatcgc agatccttga cttaaatgcg gtatttctcg agcatctgtg
     3781 cgcatatggt attgctagct cacagtccgg tcgactctag aggatcgatc cccgggtacc
     3841 gagctcgaat tagcggccac agagcttgtg acagctacag cctaagggca acaggcaggg
     3901 gagaccaagg accagaaaga gcagaggctt tttcaaagaa agagatccct ctcccagcac
     3961 ccagcgatgg cggcaaaccc cacccacagt gcctgctaag aacaagtccc acgtgagaac
     4021 aacatggccc cccgagatgc ccacagggac cccgagatgc ctgcagtgct tggctctccc
     4081 gctggccagc tgcccacctg gctcaggccc agtactcgtg agtcagccga tcaatcccaa
     4141 tgttgccagg atgatggggg cgggagtaag ggccctgggg gagggcaggg gtgggcactg
     4201 caggcgagct gtctcccaca tctggcacct gcacacagct gaggccgagc tgagaggatg
     4261 cttctgtggg ctccccctcc tcccggcacc cctcccctcc tttcactgtc ctaggacact
     4321 ctctggctgc tggagtctta ggcaaatatt taaaggggca gcaagggggt gaggagggtg
     4381 gtgggagcaa acacttccct ccctttcttc ctccctgcgc ttctcagggg ctctcagttc
     4441 agatgccatg ctgttatgca accttggggc tgaaggccct ccagatggag agggggacag
     4501 gggaacctgc cagctcatga ccgaagggca gggcccaggt gggagggggc tggggcaggg
     4561 gacaggaact ggggtggcat gttaaaggac aggaggctgg ttgggcatgg tggctcacac
     4621 ctgtaatcct agcactttag gaggccgaga tcacttgagc ccaggagttc aagaccagcc
     4681 tgggcaacat ggtgaaaact catctctata aaacaagcaa aaattagctg ggcacaatgg
     4741 catgcactgg tagtcccagc tacttgggat gctgaggtgt gaggatcacc ggagtccagg
     4801 aggtcaacgc tgcagtgagc agtgatctcg ctactgcatg ccagcctggg tgataaagtg
     4861 agaccctgtc tcacaacaaa acaaaacaaa acaaaacaaa aggataggag gttaagggag
     4921 cgagcccagg cctggactct gccacagtca cctgagttta gaggtgaagg gactttagag
     4981 accacctggc ccaaggagtg aaagggaacc tgagagaaag ggtgagccag cccaaagtca
     5041 ttggcagatt tgacctcgta aatacataga gatggctttg ggaaggcact aggaaagaca
     5101 gagaaaagag aaggagacag tcctcaaagc tgatcgtatt tgggtgagta ttattctcag
     5161 ggcaaattta ggatcagagg atgcagaaag gggagtctag aggggtagag tgtagaccac
     5221 agggtgagtg agctgattcg aggatgggga gactgggagc ccaccagtga ccagagccag
     5281 ccctgttcag ggctgtccgg gcagaagaaa gcagtgtcag acctggaatc tgccatcagc
     5341 acagcctgca attgacagac aagcccagag caaagaagga agcactgcac atgagtaaga
     5401 gcttgccacc agtggggaca gagtttccag aattaggaaa ataatcactg ggggcaagtt
     5461 tgaggttggt accagatatg tgggaggagg caaggtaagg gaaagagtac ttgaagttgg
     5521 aactggtcct tgcagggaaa tgcacattta tgaaaccccg aaaactgatg tcaaagcacc
     5581 tcctgccttg ggcagagtcc tctcagagtc tacaggtgct gcctccagaa ccctcttcct
     5641 ggagcgcatc cctatgtatc tagaaattct gctgggaaat atgatggtca gacccttggc
     5701 cacctgaaag gttcagggtg gtagaagaaa aaggaaagcc acagggcagc aggggcaggt
     5761 gccagcaagg aaggcaggca cgccaggaag acacccatgg tgagaagtgc agatggcccg
     5821 agggcaagtt tgctcaactc acccaggttt gctcttgctg gggccaagag gactcatgtg
     5881 ccagggccaa gggcccttgg gggctctcac agggggctta tctgggcttc ggttctggag
     5941 ggccaggaac aaacaggctt caaagccaag ggcttggctg gcacacaggg ggcttggtcc
     6001 ttcacctctg tcccctctcc ctacggacac atataagacc ctggtcacac ctgggagagg
     6061 aggagaggag agcatagcac ctgcagggcc gccatggtga gcaagggcga ggagctgttc
     6121 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     6181 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     6241 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     6301 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     6361 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     6421 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     6481 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     6541 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     6601 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     6661 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     6721 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     6781 atcactctcg gcatggacga gctgtacaag taaggatcct aatcaacctc tggattacaa
     6841 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6901 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6961 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7021 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7081 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7141 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7201 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     7261 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7321 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7381 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
     7441 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7501 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7561 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7621 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7681 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7741 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
     7801 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     7861 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7921 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7981 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     8041 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     8101 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8161 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8221 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     8281 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8341 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8401 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8461 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8521 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8581 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8641 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8701 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8761 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8821 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8881 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8941 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     9001 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     9061 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9121 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9181 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9241 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9301 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9361 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9421 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9481 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9541 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9601 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9661 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9721 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9781 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9841 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
     9901 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9961 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
    10021 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    10081 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    10141 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
    10201 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10261 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    10321 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    10381 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
