LOCUS       sequence-397481-        9760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2423
FEATURES             Location/Qualifiers
     source          1..9760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..207
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      58..193
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             239..841
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(239..258)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     735..755
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    884..1001
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1139..2317
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1370..2308
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2265..2285
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2328..2337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3857..4445
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3910..3930)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4328..4339)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4517..4750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4828..4949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4865..4884)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4919..4938
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4968..5103
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5123..5142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5124..5142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5152..5169)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5152..5168)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5161..5183)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5310..5765
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5397..5416)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5607..5628
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5791..5895
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5896..5964,5965..6756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6114..6133)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6927..7515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7416..7435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7669..7686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7803..7824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7839..7844,7845..7862,7863..7869)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7877..7893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7882..7904
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7901..7917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7901..7917
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7936..7956
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7938..7956
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7984..8210
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8211..8391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8438..8563
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9056..9289
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9474..9518
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(9749..9760,1..199)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
ORIGIN
        1 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
       61 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
      121 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
      181 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
      241 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
      301 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
      361 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
      421 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
      481 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
      541 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
      601 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
      661 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
      721 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
      781 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
      841 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
      901 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      961 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     1021 gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta
     1081 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg
     1141 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     1201 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     1261 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     1321 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     1381 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     1441 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     1501 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     1561 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     1621 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     1681 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     1741 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     1801 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     1861 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     1921 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     1981 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     2041 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     2101 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     2161 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     2221 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     2281 ggttcaaagt ttttttcttc catttcaggt gtcgtgaggc tagcaccgcc accatggacc
     2341 ccgcaggggc agcagacccc tcagtgcctc ccaatccttt gactcacctg agcctgcagg
     2401 acagatcaga gatgcagctg cagagcgaag ccgacaggcg gagcctcccg ggcacttgga
     2461 ccaggtcatc cccagagcac accaccattc tgaggggagg cgtgcgcagg tgcctgcagc
     2521 aacagtgtga acagactgtg cggatcctgc atgccaaggt ggcccagaaa tcatacggaa
     2581 atgagaagcg gttcttctgc cccccgccct gtgtctacct ctcggggcct ggctggaggg
     2641 tgaagccagg gcaggatcaa gctcaccagg cgggggaaac ggggcccacg gtctgcggtt
     2701 acatgggact ggacagcgcg tccggcagcg ccactgagac gcagaagctg aatttcgagc
     2761 agcagccgga ctccagggaa ttcggctgcg ccaagaccct gtacatctca gatgcagaca
     2821 agaggaagca ctttcggctg gtgctgcggc tggtgctgcg cgggggccgg gagctgggta
     2881 ccttccacag ccgccttatc aaggtcatct cgaagccctc gcagaagaag cagtcgctga
     2941 aaaacaccga tctgtgcata tcctccggct caaaggtctc cctcttcaac cgcctgcgct
     3001 ctcagacggt ctccacacgc tacctctctg tggaggatgg ggcctttgtg gccagtgcac
     3061 gacagtgggc tgccttcacg ctccacctgg ctgatgggca ctctgcccaa ggagacttcc
     3121 caccgcgaga gggctacgtt cgctatggct ccctggtgca gctcgtctgc acggtcaccg
     3181 gcatcacact acctcccatg atcatccgta aagtagcaaa acagtgtgcg ctccttgatg
     3241 tggatgagcc catctcccag ctgcacaagt gtgcattcca gtttccaggc agtcccccag
     3301 gagggggtgg cacctactta tgccttgcca cagagaaggt ggtgcaattt caggcctctc
     3361 cctgccccaa ggaggcgaac agggctctgc ttaacgacag ctcttgctgg accatcatcg
     3421 gcaccgagtc ggtggaattt tccttcagca ccagcctggc gtgtaccctg gagccggtca
     3481 ctccggtgcc tctcatcagc accctagagc tgagcggcgg gggcgacgtg gccacgctgg
     3541 agctccacgg agagaacttc cacgcggggc tcaaggtgtg gtttggggac gtggaggcag
     3601 aaaccatgta caggtacggg gtggagcccg cggtccctgg tgtgcgtggt gccggacgtg
     3661 gcggccttct gcagcgactg gcgctggctg cgcgctccca tcacaatccc catgagcctg
     3721 gtgcgcgccg acgggctctt ctaccctagt aatgaactag tccgtaactt gaaagtattt
     3781 cgatttcttg gctttatata tcttgtggaa aggacgaccg cacagagtag acttcctacc
     3841 gacgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3901 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3961 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4021 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4081 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4141 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4201 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4261 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4321 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4381 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4441 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4501 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     4561 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4621 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     4681 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     4741 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     4801 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     4861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4921 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     4981 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5041 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5101 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     5161 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5221 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5281 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     5341 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5401 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5461 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5521 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5581 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5641 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5701 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     5761 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5821 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5881 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5941 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6001 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6061 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6121 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6181 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6241 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6301 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6361 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6421 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6481 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6541 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6601 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6661 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6721 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6781 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6841 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6901 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6961 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7021 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     7081 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7141 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7201 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     7261 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     7321 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     7381 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     7441 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     7501 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     7561 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     7621 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     7681 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     7741 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7801 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7861 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7921 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     7981 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     8041 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     8101 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     8161 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     8221 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8281 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8341 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     8401 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     8461 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     8521 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     8581 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     8641 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     8701 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     8761 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     8821 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     8881 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     8941 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     9001 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     9061 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     9121 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     9181 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     9241 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     9301 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     9361 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     9421 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     9481 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     9541 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     9601 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     9661 ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac
     9721 caggtgtgga aagtccccag gctccccagc aggcagaagt
//
