LOCUS       sequence-397541-        8467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AjTn6022 proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Faure G, Saito M, Benler S, Peng I, Wolf YI, Strecker J, Altae-Tran 
            H, Neumann E, Li D, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     Modularity and diversity of target selectors in Tn7 transposons.
  JOURNAL   Mol Cell. 2023 Jun 15;83(12):2122-2136.e10. doi: 
            10.1016/j.molcel.2023.05.013. Epub 2023 Jun 1.
  PUBMED    37267947
REFERENCE   2  (bases 1 to 8467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.05.013"; journalName: "Mol Cell"; date: 
            "2023-06-15- 15"; volume: "83"; issue: "12"; pages: "2122-2136.e10"
COMMENT     Sequence Label: pSCT147: pHelper(AjTn6022)
FEATURES             Location/Qualifiers
     source          1..8467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      6371..6418
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6589..7380)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(7381..7472)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7520..8258)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 tttacacttt atgcttccgg ctcgtatgtt ggtcgacgtg gagatatacc atgtttaact
       61 ttaataagga gatatacatg aaaagaatca gtgatgaata ttccccagtt aggaaatccc
      121 aaactgtata tgggagtatt tcagtaaatt atgcttttag aggggaaaag acgatttggt
      181 gtgaatctac tcttgagcgt gattttttga ttaggcaaga attcaatagc aatgtcatag
      241 atatcgtaag ccagcctatt accatcccat atataacaga gttgggtaat gaatccactt
      301 atactcctga tttcctcgta cagttttcca gtgcaaattg tggtgatttt gaggaaattc
      361 cctgtccact gttaatagaa ataaaaccta gaaagaaact tatagaagat tgggataaat
      421 taaagccaaa atttaaggcg gctcatactt ttgctaaaga aaaaggttgg aaattcaaga
      481 ttataaatga atcaaggtta tatgatcagt attgggaaaa tattaatttt ttgaaaagat
      541 ttagacgttc tcatgtggat gtctatgatg aacaaacttt aattgagact ttagatagtc
      601 ttggtcaatc gactattaat caattaccgg cacatatttt taaggataaa actaatattc
      661 ttcgaggtat tcatcaagta tgggcattgg tcgcaaaacg aaaaatcatg tgtgatttat
      721 attgcccctt aacagcagag acggtgattt ggacaaatca agatgatgtg cttacaagga
      781 gatttttaaa tggttcagac tgatactgta agtattgacc gaagtcggat catactcaaa
      841 ccaaatgtaa ttgttaagta tgaagggcaa ccgtataaga ttgctaatgt tttgaatgcc
      901 aacgatatcg tgatttctag tttagattct gtgcgttgtt tacaagtaag tgcagattct
      961 ctacaagtat ttgaagctga aaataaccaa actaaagata taaataaagg tgattgggat
     1021 attacgcatg aagcgtggaa aatagcttta catcgttatg aaatcatcaa gccgttgatt
     1081 aagtattcaa caaccgaatt agtcgagcaa cgagcaagtg aatatggcat taaccgttcc
     1141 accttatgga aatggttaaa agattttaga gaaaataatt ctatcttagt gttagttcct
     1201 aaaaagagag gttggacgac caagaagtct aggttgtctc ctcaaattat gaatattatt
     1261 aaccaaggta ttcaagagga atatctcaat gccagaaagc ctagtattgc aaaaactata
     1321 gattttatta aagcagaatg ttctcgcctt aaactagaag caccccatga caattcaatt
     1381 cgtagaaaaa tagaagcatt aaacgattat atggtgatta aggctcgtca tggttctaaa
     1441 gcagcacaag ataagtatgc atctgctgct ggtgagtttc ctaatgctga ttatcccttg
     1501 gcatatgtac aggtagacca cactccacta gatattgaaa ttgttgatga tgaatttaga
     1561 gaggcgattg gacgaccatt tctaacatta gctattgata taaatactcg aatggtggtc
     1621 ggttattact tgtccttaga agcccccagt gcaacatctg ttgccatgtg tattgcttcc
     1681 agtattttgt caaaaaaacg gaagctaatt gaattggata tagatgctga ttggcaagtt
     1741 gaaggtctta tggattctat acatacagat aatggtcctg actttagaac taaccatatc
     1801 agtcgagctt gcttaaaata tgggattcat tgggagtttc gtccgattgg tggggcaagg
     1861 tttggtggac atatcgaacg tttgattggt gttgttaaca ttgaaatgca catcttggat
     1921 ggtacaacct tctcgaatat ccaagaacgt ggaacctata attcagcagg tcatgcctgt
     1981 atgacactaa aagaactaga atattatatt gtttattgga tcactaaagt ttatcaccaa
     2041 aagaaacact ccaaactcgg tatgtcacct ttacagaagt ggcaagatgg tgtttgggga
     2101 acaaaaacta gtgttggaac aggtttaaaa gagagagttt cggatgaaga tacattattt
     2161 attgatttcc ttcccgaatt tgaatcaaca attcaacgtg taggtgtaca gaaagacaat
     2221 ctattttatt ttgctgattg tttacgacaa tgggttaatt gtgttgatcc cgaagatgta
     2281 aataaaaaga gaaaaaagaa atttttgttt aaacgtgatc caagggatat ttctcttatt
     2341 tggttttatg aacctctgtc caatacatat tttaaagtgc ctactgctaa gcgagaaata
     2401 ccaccaatta gcttatttga atacaaacaa gtgcagaaat atcttcgtgg cgagcaacat
     2461 gataatcaaa atcaggatga gatttatcag gcaattcttc atttacgaga gcaattgaaa
     2521 caatcccgta gtctgactcg gaagcaacgg agagtaaatc aacgtaaaaa ggaaaatgca
     2581 aaagcaacac atcatttatc tgtaaaaaat gaacctgaaa aaccatctat tgtcgaaaac
     2641 agtgttcagc cgactaatga tttatggagt attccattga cggcatttga agatttaagg
     2701 ggataataat gcaaaaatat gaacatcttg cagatgatgt acttgaagtc atgtctctaa
     2761 gtgatagtga tcgtatcaat attttatttt ctgatcgttg gattggttat aaaaaagcgg
     2821 tcactatttt ggatacttta actgatctgt tgaatcgccc taggaagctt cgcccagaat
     2881 gtttattgat tgtgggtgat tctaatatgg gtaaaacgac cattatccac gaattcacaa
     2941 agcagcatta caccaaaaca gtgaatgaca tagatatgga gctgctgtct gtaaaaaaac
     3001 ctgttttaac catattagcc cctgctaaag cgaatgtaaa agaattctat atcaatatat
     3061 tgaatcattt ctttgtccca tttcgtccta ctgatcctga agcaaagtta cggaatcaag
     3121 ctgtccatct aatgcggaag tatgaaacaa aaatgttgat tattgatgaa atccataatt
     3181 gtttaacagg ttctaccaaa cttctttccg aagtgatgaa taccttaaag aatctaagta
     3241 atgatctaag tctgaatatc gttggcgtcg gtactcgtga agcagttacg attctacata
     3301 ccgatgctca atatgcaagc cgttttgatg tagcaatgtt gcctaaatgg gaactgaatc
     3361 aggattttct aagactatta ttaagttatg tacgattatt gcctttaaag aaacgttctg
     3421 atctagcatc tcaaagactt gccaccctta tatttgaaat ttcaggtggg aattttggtg
     3481 acttaaacag attgttgatt gagtgtgcta aagaggcaat cgtaaaaggt aatgaagaaa
     3541 ttaccgaaga aattgttaat aaatttaaat ggcttaaacc aaccgagggt atgaggaata
     3601 ttagaaatat aaatcttaac atggtttagg tattctactt ttcataggaa aatagccatg
     3661 caccaagtag aacaacgttg gtatattcgt acccctattc aagagggtga agtattttcc
     3721 tcttggctca tacgttctgc tttggattta ggctgctcac cattaacttt agtgaatctt
     3781 ttgtgggaaa aatggagagg attaacgata gatattgatc gtgggttaaa tgaagaaaaa
     3841 ttagacatat tgttaaatca ttgttttgtg gaacagcatg aagttcagaa aactatgtta
     3901 agcagttatt tccctgcttt gattccaaaa aattataaca aaaatcagcg tattccttgg
     3961 atattggctc taggtattcg aaatcgttca aatgtgtcag gtcgtcaaat ttgtacgcaa
     4021 tgtctaagtt ctacccatac accaccatat ttgcgtatca agtggcgtat ggggtggcat
     4081 agtggctgtg atattcataa aaccatatta atagatcatt gtcctcaatg cagaatcaca
     4141 ttacaaccta taaaaattga tatagagcat ggatcattag caatttgtac ttcatgctat
     4201 gctgatttaa gtacactcat tcaacgtcca gtagaaacgg atgcattaag atttcagcat
     4261 caagctgatc aggtcttaga gtcacaattt gggcgatata accaaaagga tatttcatca
     4321 atagagtggt ttgagattgc tcgtatatgg ttaagtttta ttcgatcccc tttaaatatg
     4381 aagagtccta accttttgca aatgtttgaa agtcttaata ttgatttaaa tatagattat
     4441 cctattacac cagtagcctt tgaatttctg tctgttcagg agcgtgaaaa attattatca
     4501 attttaaata aaatcatggc attaccttgt gatttatttg ttgatcgctc tttagaatat
     4561 catgtaagtc attcttattt ttgggataaa agaaaaaaac tgcctacgca gcttcaacta
     4621 atgaaagata aaatgttgaa gccaattaag attattttgc cgaaagataa cgccatagcc
     4681 atgagtaaac ccaaatctaa aaaaagtgta caacggaagt ggttaaggct attgagaaag
     4741 ctacagaaag gggggaacag gtgactttag ctcaattttc ccataaagaa ggctgtagtg
     4801 gttgtgacag ggctgatata gataaattca aaaaaagata taagggcgat aagtattgtt
     4861 caacttgtta tgcccgaatt tttaaaaaaa gagcttgtcc aaaatgtggt gaatatgcca
     4921 gattgccaaa aaatgatgat caagcaattt gtaatgaatg catcaaaaaa cagccttgta
     4981 ttcgctgtaa tcaaactaag aagccgatag ggacactcac cgaatatggt gtggtttgta
     5041 atagctgttc tgtttatttt cgcccaattg agcgttgtga gaggtgtgat acaccatcac
     5101 aaaaattgac acatatttca cgatttggtg atgatttaag ggtatgtccc aaatgctcta
     5161 ctagagatta tgaaacatgt ccctcttgtc ataaatatcg cttattagag caagatgata
     5221 caggagctaa gatttgtaaa aaatgtagga ataacgccaa aaagaaatgc aagcaatgtg
     5281 atgtcttgat accagcaggc tgtcctgatc tatgtaacaa ctgttactgg cataagaatc
     5341 tatgggagaa agctagacga aatattaaag cattccaatc ccaacactta caggctcaat
     5401 atgagcaata tttaatttgg ttagaagatg aggttggtgc aaataaagca gcactgtata
     5461 tcaataaaca tacccatttt tttatcaaaa ctgaagaatt atggcttgat gcaattccta
     5521 cagcagaaca gttattggct gttttaagaa ccagtggact tagaaaattt gagttggttg
     5581 catcttggct agatgaagcc catcacatta aggttgcatt agaggataaa gacttttgct
     5641 ctcaacagga tcagatagaa aaactgattt caagccttcc tcacccctct actgcttatg
     5701 atgtggttat atcttataaa gatgagcttg atataaaaat gaaagatgga aagaccagta
     5761 tccggtccat aaaactggca ataaagcctg cggttgctct tatgcactat gtttgtgcat
     5821 cgggagctac attacctaat ttaaaccata taaaagccta tcttatcgac ttttctggtc
     5881 aggctgctgc actaacaggg ttcattaatt ttttgaataa aaattttgat acatctatag
     5941 actatttggc ttttaaaaaa tcaaaaaatt tcaatgaaaa acgaaaaaat aaagtagaaa
     6001 aagaaattgt ccaatgggtg gataagcctt tggagaataa ggaagatgta ttaaattggg
     6061 tgaagaatgg gctaagatat tttcataatg tatcttatgt ggagtctcta aaagtcaaat
     6121 ttgagatgat tacagaagct gatgatggat atgaaatttt attgcagaat cattcttatt
     6181 ggttgccaaa aaacacagga gatttaaaaa gatagttttt ttgtgattat actgtgctat
     6241 ttcaagctaa tttattgtac aagttggttt gtttatctaa ttcttctaaa agaactgtcc
     6301 ataacaatat ggggcaggct gctgccaccg ctgagcaata acctaggctg ctgccaccgc
     6361 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     6421 gaaacctcag gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt
     6481 aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc
     6541 atcgtggccg gatcttgcgg cccctcggct tgaacgaatt gttagacatt atttgccgac
     6601 taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga
     6661 ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg
     6721 atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt
     6781 gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc
     6841 agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc
     6901 ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg
     6961 ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat
     7021 acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata
     7081 acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc
     7141 gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt
     7201 ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc
     7261 gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc
     7321 gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg cttccctcat
     7381 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7441 catatttgaa tgtatttaga aaaataaaca aatagctagc tcactcggtc gctacgctcc
     7501 gggcgtgaga ctgcggcggg cgctgcggac acatacaaag ttacccacag attccgtgga
     7561 taagcagggg actaacatgt gaggcaaaac agcagggccg cgccggtggc gtttttccat
     7621 aggctccgcc ctcctgccag agttcacata aacagacgct tttccggtgc atctgtggga
     7681 gccgtgaggc tcaaccatga atctgacagt acgggcgaaa cccgacagga cttaaagatc
     7741 cccaccgttt ccggcgggtc gctccctctt gcgctctcct gttccgaccc tgccgtttac
     7801 cggatacctg ttccgccttt ctcccttacg ggaagtgtgg cgctttctca tagctcacac
     7861 actggtatct cggctcggtg taggtcgttc gctccaagct gggctgtaag caagaactcc
     7921 ccgttcagcc cgactgctgc gccttatccg gtaactgttc acttgagtcc aacccggaaa
     7981 agcacggtaa aacgccactg gcagcagcca ttggtaactg ggagttcgca gaggatttgt
     8041 ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag tccggctaca ctggaaggac
     8101 agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt ccccaactga
     8161 cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta
     8221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgctctaga
     8281 tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt
     8341 tgtaattctc atgttagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     8401 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     8461 gttgcgc
//
