LOCUS       sequence-397566-       12114 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  insertional mutagenesis vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Pazour GJ, Sineshchekov OA, Witman GB
  TITLE     Mutational analysis of the phototransduction pathway of 
            Chlamydomonas reinhardtii.
  JOURNAL   J Cell Biol. 1995 Oct;131(2):427-40. doi: 10.1083/jcb.131.2.427.
  PUBMED    7593169
REFERENCE   2  (bases 1 to 12114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb.131.2";
            journalName: "J Cell Biol"; date: "1995-10"; volume: "131"; issue: 
            "2"; pages: "427-40"
COMMENT     Sequence Label: pGP505
FEATURES             Location/Qualifiers
     source          1..12114
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(167..186)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      980..1568
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1469..1488
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1722..1739
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1856..1877
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1892..1897,1898..1915,1916..1922)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1930..1946
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1935..1957
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1954..1970
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1954..1970
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    7018..7029
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    7069..7080
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(10992..11009)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10992..11008)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11001..11023)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11221..11676
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11308..11327)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11518..11539
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11693..11712)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11812..11834
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11872..11890)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11958..12062
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12063..12114,1..17,18..809)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
       61 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      121 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      181 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      241 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      301 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      361 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      421 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      481 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      541 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      601 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      661 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      721 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      781 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      841 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
      901 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      961 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1021 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1081 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1141 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1201 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1261 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1321 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1381 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1441 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1501 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1561 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1621 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1681 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1741 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1801 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     1861 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     1921 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
     1981 attctttcgg acttttgaaa gtgatggtgg tgggggaagg attcgaacct tcgaagtcga
     2041 tgacggcaga tttagagtct gctcccttat cgggaagcgg ggcgcatcat atcaaatgac
     2101 gcgccgctgt aaagtgttac gttgagaaag aattctcttc aaccaacccg tttcacttgt
     2161 cgctgtaaaa gcaacattag tgtttgctac ttgcgacttc tatagcggct gtgcgaccga
     2221 aaacgaggga gcggccgttc gggtgtgacc gttagtgggc cggtcgctct gaagtcgctt
     2281 ctttggtcgc tgagagctcc accattatca aggacatgcg ctaaactgct atctactcgc
     2341 gaggtacaca ggtgctttga ccgcgcctgt gtccccgcgc agtgccccgc gcgtttcagt
     2401 cgcgcactcc ctagtccgac tcttgcccag cgccccctct tcccggccac agtcgaggta
     2461 aactcctctc gcagcccgac ttacagccaa gggctcccat tcgcggtctg ccatctggcc
     2521 gcccaactgc tgttgcggcc tggccgccct ggggcactag cagctctcca tggtccttgc
     2581 ggccctggct cgactggtcg cccctgggag tctaagcccc cttgttgccc cacgccgtgc
     2641 cagcccatgg cagcacctgc ccttgcctgc cctgctgcac aaagtgatgg acacaatgca
     2701 agtcgcttgc ttaatttaca actgtgcggc agggggtgtc tatatgtagc tttacctgca
     2761 cctcctgcac cttcgccggt tggcaccgat ggcgtgctgc catacacagg tcgcactctc
     2821 acgcgccaaa acccccttat gattatgtat cgcattgcat caaacaagct ctcaccaaaa
     2881 cgggctaacg ccccggccct ctgcttcatg catggcaccc accctcctga ccaacccacg
     2941 ccctaacgtg ggttcagccc acggagggtc gtacttctcc ccacgcgcta aacgctgtca
     3001 ggcactttcc tgacataata taataaccgc atggaaagcc tccctatgtc acatgaaatg
     3061 ctgcgtcatc aatacatcat ctcaacggcg gtgcctgact ccaaaactag cacgtgcaca
     3121 cactctctcg ggcactctgc tgtgccacta ctgctatccc ctgctgcagt caatcagtaa
     3181 tatccttgcc atgcttcctg ccgtttgcag gcttgcacaa ctcatttgcc cactgggaaa
     3241 ctgacctaca cattctatct tttaactgcc tactgttgcc aaactcgttt gtcccctcag
     3301 cgcaggtttt aaactgccaa cgcgtcccag tgcgccgcag ccaagccggg cgcactctgt
     3361 ctgaccctgc ggtcctgcct agctctcaca cacaaccaca cagacgcaca cttccgtcgt
     3421 caaactgcac aaaaggccgc tgcggccgtg gcctgtgcca gcccttatcc ggctcacaca
     3481 cgctcccacg accgctgccc ctgcccctgc cctggcatcc gcccagatgc ctggattcgg
     3541 atccaatcct gctggtcgca tgatccaacc gtggccacaa tctctagcga tctagaactg
     3601 gatctggcgg tccttgctgt agcccatggc ctccaggaac ggcacgcagc agtgctcgat
     3661 catgccgtgc ggcccgcaca tgaggctgag gcagtcctcg cccgtgctgg cgaagaggtg
     3721 ctccgtgaac atgtccttgc tcacacgccc cacgtcgtac ttccaggtcg ccgcgtcctt
     3781 gggctgtgac accgtgtacc tgaggatggc gggagggggt gggggagggg ttcggtgttg
     3841 gttggttcgt tgacggttgg ctcaggctgg gacggaaggc gaagccaaag ctgcggctgc
     3901 gcgcgccgta cgtgtggccc ccgggtgcgc cgctcaccac agccggaagc gctcggggtg
     3961 gttgtttgcg agctcgtcca gctcctcgcg cagcagaatg tcgtcctcgt gtgtgttggc
     4021 gaacagcagc gccagcctgc aggtgggaag ggggttgtgg ggtgtgtgtg ttggtgtgtt
     4081 ggtgtcagag tgggtgtgcg tgatggatga tggacacagg tgtgggcgcg ggtgtatgtg
     4141 ccggggacgt gcgtgtggcc ctttagtccc tggctgtagc catgcccatc cactgccttg
     4201 ctatgctatg gcactgtgat acggcggcag caaagcgtgt catcagtcag ctaacagcca
     4261 acggcaatga ggccgcttcc tctcccgctt ccccccgccg ccgccgctgc tcacttggtg
     4321 ttgtcctcgg ggtcgcgcag tgcggccttg atgaccgcgt agcagggcgt gatgcccgtg
     4381 ccgcccgcaa caaacgacat gtgactggcg tgcttggtca cctgcaggga ggtaggggtg
     4441 tgtgtgtgtt agctatctag cgtgtgtgac aatgtgccgt aagaagctca ccacaatgtg
     4501 tgtgcattta aatgtgcttg tgtggtaggc ttactcattc tcgcggctac aggaccgccc
     4561 gttcctgttg cgcgcacctt gccgttgagc gtgtagcttc cgcggccgtt gtacacgaag
     4621 tgccccagcg gccccttgaa ctccaggcag tcgccgatgg cgagcgagtc gaaatgctgg
     4681 ctcatcttgc cgccctcggg gtaggacgcg tgctcgccct taaagtacac ctgtgggggg
     4741 ggggggggsg gggtgtgtgg ggtcaggtgg ggattgtggg gtgtggggca tggcgcggtc
     4801 agggggcaca cacggtacaa aagatgaatg acgcaccaga aaacacctac acacacatgc
     4861 acacacgtcc ccacccccac acacaggcgc acacacgtcc ctcacacata cacacacaca
     4921 catacacgca ccttgatgag catgtccagc cggcccttct cctcgtcccc gctgatgggc
     4981 gtgtaggcgc gcatcacgtt ctcgccgccc acctgcgcgt acacgaacac gtgcttgccc
     5041 accggcaggc ctgcgcatcg ggggagggga gggggtggga ggtgcagtga gtggtggcca
     5101 gtgagtggtg ccgcggacgc gcaaccgaca cgaagtcagc ccgctccttc cccttctgct
     5161 ggctgctctt gttcttgccc ccttccccca cacaaatcaa ggcacacact cagcagggca
     5221 gccaagacgt gcgtactacc gtatcattag tatcataacg cgcgcgacac cacccaccga
     5281 tgcggtgctc cggcgagggc aggccgaacc ggaatatgcg cgtgttgcgg ttgagctcaa
     5341 tccgctccac cagcggtagc ttcaccttgg cgcgacccgt cagcacaacc agagggtcgg
     5401 cgggcggcgc cgccgccgcg gtgccgttgg cagttgccgt gccgttgccg ttggcggttg
     5461 ccgtgccgtt ggcggttgct ggttttgatg ccacgaggtc accgatgtag tactgggcca
     5521 gcatggcctt ggctttggag ctgtgtgtgg gggatgtggg ggcgcaggtg gggagatggg
     5581 gtgagatggg atggggacgg tggtgtgcgt ggtgtgtatg ggacggtggg ttggatgcca
     5641 cgccatgcgc ggacccctta tacacgcctg ccgctgatcc tagccccgca aaacgtcccc
     5701 tctctcgtac acgcccccaa aacaccaatc acacacgcac aaaacacatg cacacacacg
     5761 ttctcgcccc ctacccacct gtggatggcg ttgaactcgt ctgtggcgtc cgcgcccgca
     5821 gtgatcagga tggactcggc gccacccggg tggtcattca ggtagggcgt ggcgtcgtac
     5881 acctggagag ggagcggagg gcggggaggg gggggggtga aaggggttag aaagctgggg
     5941 tccagggtgg ttcagggttt cgtgtgcacg tcagcacggg ttggggtgca gctgctgggc
     6001 acgcacgtac ccacaaccac gcctctcgcc cttcctacaa cctcaaccac aaacccctag
     6061 ccccagacca gccaccagac cagccaccac gccatccgct ctcctcaccc ggccctcgtg
     6121 cacgaaccag cagctctcct cgctcgcgtg ctccgccacc tcctccagcg tgtactgctt
     6181 ggggccgcca ttcgccagca gcgccgccgg aggcggcggc ggcggtgccg ccgccgcgcc
     6241 tccgccgtcg cgcgccgccg ccacggcctg cgccaccagg ttgtcctcct cgcgccagcc
     6301 catgttgccc ttgtcgccca gctccacggg ggccgggtgc tggaagcgca ggcccatgac
     6361 gcccgtggct gggtccacct ccgggtggat cttgatgctg cgcgtgttac gtgggggaca
     6421 ttcatgatta gtttagtggg atggggtggg gaggggcggg ccgacgtgtg gaaccagggc
     6481 tgtcacgcag gtgttcgggg aaatgcgatg agcggcgctg cgatgctgaa cattgcacaa
     6541 aagggcgcct ctcgccgcct ccctcatcgc cgcctccccc ccttccacac acgcacacac
     6601 acacgctcac cggaagtagc agttgttcat catgcccatc aggttccagg tgatcaccgc
     6661 cggctgcgtg ttcatggtct catcccaggc gcggcacagc acctccttgg cggacaggaa
     6721 gtcaaacgtg ttcacctgtg tttttttggg ggggggggca gagcgaagca gtgggtccgt
     6781 ggggctggag ggacgcaaag gcccatcccg ccatcccaac cgacatcgcc catttcgccc
     6841 acaggcgccc actcctcctc gctccggcgc ctcacctcca gcgtccagtg cacccagcac
     6901 cagtgcttgc cgtactcgtt gggctcctcg aagcgctgga tgtcgcccag gcgccaggtc
     6961 ttgccgtcgt ccagcgagac ctcgcagcgg atgatcttgc ggccgccgcc tgcacatgat
     7021 gaggatgacg acacagacca acgccagcaa ataacttaaa tatacttcgt catcctcatc
     7081 ttcagtcctg ccgttactta actagccgct cacacgtgtc ccgttccaca cgtcctaaat
     7141 cgcatgtacg gtatgatgcc tgccagtgcc acgaccaaac ggcccgaaac caacgacgtt
     7201 ggctctctca ctcgctcgct aggcagctcg ctgcccccgc ttgctgtcat tatcgctgtt
     7261 gttgctgctc accggcgtag gcgtaaccct tgatggtgta gggccggttg gcgtccagcg
     7321 ggaccagctc gtcgtgccag ggccgagcca tcgcgctgtt gatgttgagg tcgttgatga
     7381 tgaactcggg cttgtaccac cagcctgcgc gcgtgtacac ggcgagtttc aggacgggga
     7441 cttcggaacg gggatgacac gggttcgaat gatggcggtc acatgcacac acacaagatt
     7501 tctgcggaaa tctttccctg tttcctaccc tgtgctctcc aatacacaca cgccacggtc
     7561 ctcccccccg cgctcaccct ccttcttggc cagctcctcg tccacgtggc tgggcagcac
     7621 gcggttgtcc atgaagtggt agaagttctg tgtgtgtagg ggtgtgtgtt ttgggggggg
     7681 gatggagagg gagtggcggc acaggttaca agggtatcgt ggtagggccg aagcgtgtaa
     7741 gcatagttac gccccacaag ctttgatgcc aggctgggag ctacatgcca gcccatcggc
     7801 ctgaatcaat tcgcccgcac acacccagac aactacatac acacacgttg tgctcgctgg
     7861 cttgatggct tcgcattgtt agccttaaaa gcattctcga aacccacacg cccacgcacc
     7921 tgcgactccg tgtccatgac ggtgatctcg ctcagccact tgaccatgcg gccgccgatg
     7981 aagcccggga tgatgatgcg caccgggaag ccgtggtcgg gcgtcagcca cctgcgcagg
     8041 cagcaggcag caggcgggga ggggcgggga ggagcgggga gggagggagg gagggggtga
     8101 ggaagctgag cagcacggca cgacggctat tctgtgcggc aatcttgcgc atccgtcgcc
     8161 atcgccattc ggacgagctc caggcctccg acccgtgcga cccagtgccc agcctagcgc
     8221 caacctcccg ctgccctccc cgcgcccccg ccccccgaca cacgcgcaca caaacacaca
     8281 cagcctagtg tttgccttgt gcgctcgaac acacacgcac acgcacagcc tattctttgc
     8341 ctttcgctct ttcacacacg cacacacaga gacacacaca cacacacaca cacacacaca
     8401 cacacatacg cacacgcaca cacctgtggt tctgcttgta tgcgatgata acgtccgagg
     8461 cggggtccat ggccttggcg tacgtcagag aggtgccgta cgagccgtcc tcgccacgtg
     8521 gcagctcgcc cttggggccg cggaagcaca cgaagcgggc gccctcggcc ggcgtctgca
     8581 gcagcaggcg tgggaatggg gagaagcaag gcgggtggcg gggatcaggg aaatgtgctt
     8641 gcgttcccac acaggcatac atgcactcac accacgccac atcaagcccc tgtcttgcaa
     8701 ttcctcgccc ccgccatcac caccacacgc gctctgcttc cctcctcccc tccttccttc
     8761 cctcccatcc ctccctgcca tccctcccca tccctcgtcc ctctctcgtt cccccctctt
     8821 cccacggtta tacgcggagc tcgcgctgca tttctgggtt gggtgacgac tccttgactc
     8881 ttgggactta ttgggctcgg gcacatcacc cattcacccc cccactggtt tggtttagtt
     8941 tgggctggta tttacaaccc cctgtcagct atacacacac acacacacac accttgatgc
     9001 cggcgtgctg caacaggtcg cgcagccgca cgcccgtcca gtaggtggtg ctggtggcac
     9061 aagggcccca gttgaagcca atgctcttct tcagcatgtt ctcctccttg cggcggttgc
     9121 cagcgcacac cagcgtcacc gggaaggtga cggagggcag cgccaccagc tcgtccatgg
     9181 tcaaagtcaa cggcttgttg accaggccgt tgatctcgat gcggtgctca tcccagcgga
     9241 tgcgcggcgc ggcgccgtgg ttgcgcacga agtgtacggc cggcggcgtg atgaaaccgt
     9301 actgttggtt tgtgtagggt tggttggttt gggggattgg aggggaagag gagagcggga
     9361 gatggtcagt gaggacgagg ctgtagctgg actgtgggtt gacgcgtctc cacgtacgcc
     9421 cagcctgccg tcacccgtta accccgcccc tcgccccagg ccccaggcct caaacccgtg
     9481 ccacccccac cccacgccca accaccgccg cataattctc cctaaattat caacccagcc
     9541 agcttcgagc catggctgtc agttccctcc tctgctcttt gttcactaac ctaacctcaa
     9601 gctcgcctcc cccctgcaaa tccccctcac ctgcatgagc acggacatgg gcggctcgca
     9661 gttgagcggg tgccggccgg tgaggcgcag gatgcggggg tcgcgccgca cccagttgtc
     9721 gggcgtgtcc tggtcgcgct tgtccacagc tgagggaaga acgtgctgcg accagtcggc
     9781 atcctcgggc gagagcggag gctcatacgg agcgccgagc tcgaccgctt gcgatacgat
     9841 agcgtcgggc gcctgcacct tgccgctggg cacgagtgcc acgggctgct cggcaacggt
     9901 catactggca ggattcggct gagggtcgta aagctaatca agtgaagctt cgtgtatggc
     9961 tttggctaca aagcagccct tggccaacaa tgcaaacgca gacggactct cgagcccggc
    10021 tgtattagtg tggcctttgc tttcaacgct gtgctatata gcgacaagtg aagtggagca
    10081 ccaagcagga ggcgccacga agagagacgc aagttcaacg tggttatcga cctaactatt
    10141 gctgcacacg tgcacggtcg cacaagacta tcgattggtc ggttcctgta tcgtcttgct
    10201 tgcgacccga agccctaatg cgcgcacaag agcccggggc cgggagcgcc ccccgacaaa
    10261 agttcgtacg gcggggtcgt ttgaaccctt cccttgaacc ttcaagccac aatgcagcgt
    10321 cctatggaag cgaatgcatt ttcatgcgcc tcggtctcca gctcagaata gcgcgagagg
    10381 cggacgcccc aaacaacccg gggccatcga gctccaagtc gcgcggggtc actcgcaccc
    10441 taccatatag tccaacgctt cccaagggct gaccccccgt tgcgccgaac agcgctgagc
    10501 aatatagccg gtgcttattg cacagggcag cgagacattt ctggcgcgcg gccgcttttc
    10561 tagccacccc tccagggtgt ctgaaggaac gcccctccca cccacccacc ccccaccctg
    10621 agccccgtcc actatcgcgt acatgctcga taagggaaac tcatagatta cccggacggg
    10681 gatgtgaggg cgccgcgtca ggacttttgg catgaggcat cagaggcagc agaggagcgg
    10741 gggctggcgg ggcacctgta catgtaccca ttcactgtga ttcgtgggcc gggattctag
    10801 ccaccacact cgggacaatg cttctaatgc ttctcaccac cgtctgctct agcttgtacc
    10861 tggaattata cgtaccgtta cccagccata cagagcccac gccctcgacg tctcgcctcg
    10921 agcctgatgc cccttcaacc gcaagcctgg cgtgcatatc tagagtcgac ctgcaggcat
    10981 gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
    11041 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
    11101 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
    11161 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
    11221 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    11281 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    11341 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    11401 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
    11461 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    11521 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
    11581 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    11641 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
    11701 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
    11761 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
    11821 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
    11881 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
    11941 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    12001 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    12061 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca tttt
//
