LOCUS       sequence-397574-       12842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac Vector expressing FAM134C-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12842)
  AUTHORS   Hoyer MJ, Capitanio C, Smith IR, Paoli JC, Bieber A, Jiang Y, Paulo 
            JA, Gonzalez-Lozano MA, Baumeister W, Wilfling F, Schulman BA, 
            Harper JW
  TITLE     Combinatorial selective ER-phagy remodels the ER during 
            neurogenesis.
  JOURNAL   Nat Cell Biol. 2024 Mar;26(3):378-392. doi: 
            10.1038/s41556-024-01356-4. Epub 2024 Mar 1.
  PUBMED    38429475
REFERENCE   2  (bases 1 to 12842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01356-4"; journalName: "Nat Cell Biol"; date: 
            "2024-03"; volume: "26"; issue: "3"; pages: "378-392"
COMMENT     Sequence Label: pAC150-FAM134C-GFP
FEATURES             Location/Qualifiers
     source          1..12842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   299..333
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    667..700
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        850..1229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1230..1433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1384..1404
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1430..1454
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2618..3613
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    3649..3783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3686..3705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3740..3759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3812..3845
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4487..5665
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4718..5656
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5613..5633
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5726..5750
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(7189..7191,7192..7905)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7189..7191,7192..7902)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7231..7252)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7492..7511)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(7908..7932)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7985..8026
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(8064..8081)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8070..8294
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   9114..9176
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9214..9233)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9215..9233)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9240..9257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9240..9256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9249..9271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9394..9696
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9575..9594
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             10045..10839
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10045..10839
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10099..10118)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10709..10728
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(11089..11880,11881..11949))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11712..11731
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12073..12661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12562..12581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12815..12832
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttttaacc
      301 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
      361 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
      421 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
      481 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
      541 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
      601 agatatcaac tagaatgcta gacctttcgt cttcaagaat tccgatcata ttcaataacc
      661 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctgaa gaggagttta
      721 cgtccagcca agcttaggat cccgggtacc gatggccggg cctcgaggag cttggcccat
      781 tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac
      841 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      901 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      961 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     1021 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1081 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     1141 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1201 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     1261 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     1321 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     1381 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     1441 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     1501 gggaccgatc cagcctcccc ggctagccgc cgccaccatg aaaaagcctg aactcaccgc
     1561 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1621 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1681 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1741 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     1801 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1861 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     1921 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1981 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     2041 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     2101 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     2161 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     2221 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     2281 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     2341 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     2401 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     2461 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtcg cgtctgcgtt
     2521 cgaccaggct gcgcgttctc gcggccatag caaccgacgt acggcgttgc gccctcgccg
     2581 gcagcaagaa gccacggaag tccgcccgga gcagaaaatg cccacgctac tgcgggttta
     2641 tatagacggt ccccacggga tggggaaaac caccaccacg caactgctgg tggccctggg
     2701 ttcgcgcgac gatatcgtct acgtacccga gccgatgact tactggcggg tgctgggggc
     2761 ttccgagaca atcgcgaaca tctacaccac acaacaccgc ctcgaccagg gtgagatatc
     2821 ggccggggac gcggcggtgg taatgacaag cgcccagata acaatgggca tgccttatgc
     2881 cgtgaccgac gccgttctgg ctcctcatat cgggggggag gctgggagct cacatgcccc
     2941 gcccccggcc ctcaccctca tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc
     3001 ggccgcgcgg taccttatgg gcagcatgac cccccaggcc gtgctggcgt tcgtggccct
     3061 catcccgccg accttgcccg gcaccaacat cgtgcttggg gcccttccgg aggacagaca
     3121 catcgaccgc ctggccaaac gccagcgccc cggcgagcgg ctggacctgg ctatgctggc
     3181 tgcgattcgc cgcgtttacg ggctacttgc caatacggtg cggtatctgc agtgcggcgg
     3241 gtcgtggcgg gaggactggg gacagctttc ggggacggcc gtgccgcccc agggtgccga
     3301 gccccagagc aacgcgggcc cacgacccca tatcggggac acgttattta ccctgtttcg
     3361 ggcccccgag ttgctggccc ccaacggcga cctgtataac gtgtttgcct gggccttgga
     3421 cgtcttggcc aaacgcctcc gttccatgca cgtctttatc ctggattacg accaatcgcc
     3481 cgccggctgc cgggacgccc tgctgcaact tacctccggg atggtccaga cccacgtcac
     3541 cacccccggc tccataccga cgatatgcga cctggcgcgc acgtttgccc gggagatggg
     3601 ggaggctaac tgaagcttcc cgggggtacc gaattcgtcg acagatctaa cttgtttatt
     3661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     3781 atcatcaagc ttaggatccg gaacccttaa tataacttcg tataatgtat gctatacgaa
     3841 gttattaggt ccctcgaaga ggttcactgg cgcgttggat ccccgggtac cgagttggga
     3901 gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
     3961 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
     4021 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     4081 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
     4141 tttaggctga aagagagatt tagaatgaca gtctagtggg agctcacggg gacagccccc
     4201 ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg
     4261 cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg
     4321 acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct
     4381 ctttgagcct gcagacacct ggggggatac ggggaaaaag ctttaggctg aaagagagat
     4441 ttagaatgac agaactcgat ttcattgcag actggcgcgc ccgtgaggct ccggtgcccg
     4501 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     4561 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     4621 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     4681 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     4741 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     4801 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     4861 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     4921 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     4981 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     5041 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     5101 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     5161 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     5221 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     5281 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     5341 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     5401 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     5461 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     5521 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     5581 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     5641 ttttcttcca tttcaggtgt cgtgaagaag cttggtaccg agctcggatc cactagtcca
     5701 gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagcaggct gcggtacatg
     5761 gctgaggccg aaggggttcc cacgacccca ggcccggctt cggggtcgac tttcaggggc
     5821 cgccgagatg tgtcaggctc ctgggagcgg gaccagcagg ttgaggcggc gcagcgggcc
     5881 ctggtggagg tgctggggcc ttacgagcct ctgctgagtc gggtgcaggc agccctggtg
     5941 tgggagcggc cagctaggag cgctctgtgg tgcctggggc tgaacgcggc tttctggttt
     6001 tttgccctga catctcttcg tcttgtgttt ttacttgcat ttggcttgat gatcattgtg
     6061 tgtattgatc aatggaagaa caaaatctgg cctgaaataa aagtgccaag acccgacgca
     6121 ttagacaatg agagctgggg ctttgtgcac cctcggttgc tcagcgtgcc cgagctctgc
     6181 caccatgtag ctgaagtctg ggttagtggg accattttca taaggaatgt tttgcttttc
     6241 aaaaagcaaa acccaggcaa gttctgcttg ctgagctgtg ggatactgac ctttttggct
     6301 gtcttgggcc gctacgtccc tgggcttctg ctgtcctact tgatgcttgt cactgtcatg
     6361 atgtggcccc ttgctgtgta ccaccgactg tgggatcgag catatgtgcg gctgaagcca
     6421 gctctgcagc ggctagactt cagtgtccgt ggctacatga tgtccaagca gagagagaga
     6481 caattacgcc gcagagctct ccacccagaa cgagccatgg acaaccacag tgacagcgaa
     6541 gaggagcttg ctgccttctg tcctcagctg gacgattcta ctgttgccag ggaattggcc
     6601 atcacagact ctgagcactc agacgctgaa gtctcctgta cagacaatgg cacattcaat
     6661 ctttcaaggg gccaaacacc tctaacggaa ggctctgaag acctagatgg tcacagtgat
     6721 ccagaggaat cctttgccag agaccttcca gacttccctt ccattaatat ggatcctgct
     6781 ggcctggatg atgaggacga cactagcatt ggcatgccca gcttgatgta ccgttctccg
     6841 ccaggggctg aggagcccca ggccccacct gccagccggg acgaggctgc gctgccggag
     6901 ctcctgcttg gtgctcttcc tgtaggatcc aacctcacca gcaaccttgc cagcctggtc
     6961 tcccagggta tgattcagct ggccttgtca ggggcctccc aaccaggccc ttctggagca
     7021 cctgcccaga gagcaacgag aggcttcctc cggtccccca gttcagacct ggacactgat
     7081 gctgaggggg atgactttga acttctggac cagtcggagc tgagtcagct ggaccctgcc
     7141 agttctagga gccacggagg ctcaggaggc tcaggaggct cactcgaggt gagcaagggc
     7201 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     7261 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     7321 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     7381 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     7441 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     7501 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     7561 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     7621 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     7681 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     7741 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     7801 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     7861 accgccgccg ggatcactct cggcatggac gagctgtaca agtagtgacc cagctttctt
     7921 gtacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     7981 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttaggg
     8041 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     8101 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     8161 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     8221 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     8281 ggtgggctct atggcggcgc gcctggatcc ccgggtaccg agttgggagc tcacggggac
     8341 agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag
     8401 cgagccgccc ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg
     8461 tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct
     8521 cgctgctctt tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa
     8581 gagagattta gaatgacagt ctagtgggag ctcacgggga cagccccccc ccaaagcccc
     8641 cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg
     8701 ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca
     8761 cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc
     8821 agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag
     8881 aactcgattt cattgcagac tggccggcca ctagtacgcg ccggctcgac atactagtta
     8941 aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa taaataaata
     9001 aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat ataataaaac
     9061 ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa acacatgcgt
     9121 caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt ctagggttaa
     9181 aagggcgaat tcgcggccgc taaattcaat tcgccctata gtgagtcgta ttacaattca
     9241 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     9301 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     9361 ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac ctataaaaga
     9421 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga
     9481 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     9541 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     9601 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     9661 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa
     9721 aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc
     9781 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     9841 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     9901 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     9961 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa
    10021 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
    10081 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
    10141 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
    10201 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
    10261 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
    10321 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
    10381 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
    10441 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
    10501 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
    10561 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
    10621 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
    10681 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
    10741 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
    10801 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc
    10861 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac
    10921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    10981 gtatccgctc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    11041 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    11101 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    11161 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11221 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11281 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11341 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11401 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11461 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11521 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11581 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11641 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11701 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11761 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11821 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11881 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11941 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    12001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    12061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    12121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    12181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    12241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    12301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    12361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    12421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    12481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    12541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    12601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    12661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    12721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    12781 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    12841 ag
//
