LOCUS       sequence-397591-        9470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses GFP-TurboID in the mammalian cytosol, lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Qin W, Cheah JS, Xu C, Messing J, Freibaum BD, Boeynaems S, Taylor 
            JP, Udeshi ND, Carr SA, Ting AY
  TITLE     Dynamic mapping of proteome trafficking within and between living 
            cells by TransitID.
  JOURNAL   Cell. 2023 Jun 23:S0092-8674(23)00596-2. doi: 
            10.1016/j.cell.2023.05.044.
  PUBMED    37385249
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jun 23:S0092-8674(23)00596-2. doi: 10.1016/j.cell.2023.05.044."
COMMENT     Sequence Label: Flag-GFP-TurboID-NES_pLX304
FEATURES             Location/Qualifiers
     source          1..9470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3279..3674
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3734..3851
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3968..4271
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4272..4475
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4426..4446
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4498..4522
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4543..4566
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(4573..4575,4576..4578,4579..5289)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4573..4575,4576..4578,4579..5289)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4879..4898)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5226..5247
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5308..6264
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6271..6297
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     protein_bind    complement(6323..6347)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6380..6968
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6433..6453)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6851..6862)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7040..7273
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7351..7472
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7388..7407)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7442..7461
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7512..7647
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7667..7686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7668..7686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7696..7713)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7696..7712)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7705..7727)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7854..8309
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7941..7960)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8151..8172
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8335..8439
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8440..8508,8509..9300)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag
     3301 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     3361 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     3421 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     3481 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     3541 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     3601 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     3661 atgtgtggga gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag
     3721 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     3781 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     3841 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
     3901 gggcccgggg ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     3961 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4021 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4081 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4141 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4201 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4261 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     4321 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     4381 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     4441 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactgtc gggatcaaca
     4501 agtttgtaca aaaaagcagg cttcgaagga gatagaacca tggactacaa ggacgacgat
     4561 gacaagggat ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     4621 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     4681 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     4741 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     4801 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4861 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4921 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4981 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     5041 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     5101 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     5161 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     5221 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     5281 ctgtacaagg ggggaggcgg gggtagtaaa gacaatactg tgcctctgaa gctgatcgct
     5341 ctcctggcta atggcgagtt ccatagtggc gaacagctgg gagaaaccct gggcatgtcc
     5401 agggccgcta tcaacaagca cattcagact ctgcgcgact ggggcgtgga cgtgttcacc
     5461 gtgcccggaa agggctactc tctgcccgag cctatcccgc tgctgaacgc taaacagatt
     5521 ctgggacagc tggacggcgg gagcgtggca gtcctgcctg tggtcgactc caccaatcag
     5581 tacctgctgg atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag
     5641 caggcaggga gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac
     5701 ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc
     5761 ggaattgtca tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg
     5821 cccaatgacc tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga
     5881 ataacaggcg atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc
     5941 gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg
     6001 gacaggaata ctctggccgc tacgctgatc cgagagctgc gggcagccct ggaactgttc
     6061 gagcaggaag gcctggctcc atatctgcca cggtgggaga agctggataa cttcatcaat
     6121 agacccgtga agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat
     6181 aaacagggag ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa
     6241 atcagtctgc ggtctgccga aaagctgcag ctgcctcccc tggagcgcct gaccctggac
     6301 taatctagag ggcccgttta agacccagct ttcttgtaca aagtggtgct agcgctaacc
     6361 ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6421 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6481 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6541 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6601 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6661 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6721 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6781 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6841 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6901 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6961 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7021 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7081 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7141 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7201 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7261 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     7321 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     7381 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7441 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     7501 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7561 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7621 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     7681 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     7741 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     7801 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     7861 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7921 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     7981 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8041 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8101 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8161 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8221 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8281 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     8341 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8401 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8461 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8521 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8581 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8641 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     8701 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8761 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8821 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8881 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8941 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9001 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9061 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9121 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9181 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9241 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9301 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9361 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9421 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
